• Asian-Australasian Journal of Animal Sciences
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• v.26 no.9
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• pp.1255-1261
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• 2013

Somatic cell nuclear transfer (SCNT) is a useful tool for animal cloning, but the efficiency of producing viable offspring by SCNT is very low. To improve this efficiency in the production of cloned pigs, it is critical to understand the interactions between uterine function and cloned embryos during implantation. Lysophosphatidic acid (LPA) is a lipid mediator that plays an important role in the establishment of pregnancy in pigs; however, LPA production in the uterine endometrium of pigs carrying SCNT-cloned conceptuses has not been determined. Therefore, we investigated expression of ENPP2, an LPA-generating enzyme, in the uterine endometrium of gilts with conceptuses derived from SCNT during the implantation period. Uterine endometrial tissue and uterine flushing were obtained from gilts carrying SCNT-derived conceptuses and from gilts carrying conceptuses resulting from natural mating on d 12 of pregnancy. Our results demonstrated no difference in the level of ENPP2 mRNA expression in the uterine endometrium between gilts carrying SCNT-derived conceptuses and gilts carrying naturally-conceived conceptuses, but secretion of ENPP2 protein into the uterine lumen did decrease significantly in pigs with SCNT-derived conceptuses. These results indicate that expression and secretion of ENPP2, which are critical for appropriate LPA production and successful pregnancy, are dysregulated in the uterine endometrium of pigs carrying SCNT-derived conceptuses.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 1996.11a
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• pp.39-49
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• 1996

Hundreds of contaminated sites have been generated due to the past mismagement of toxic substances, the lack of adequate environmental controls and ignorance of the potential environmental impacts of general activities in Canada. The general public, industry and governments have been addressing the contaminated sites with a number of cleanup responses. Environmental protection and remediation have become top priorities for the public and private sectors alike in Canada. Between the late 1980s and the early 1990s, the Canadian Environmental Protection Act and Canada's Green Plan were followed by provincial and territorial laws and policies to regulate contaminated sites. The National Contaminated Site Remediation Program(NCSRP) was initiated in 1989. It has been administered through bilateral agreements between the federal and participating provincial/territorial governments. They have committed a total of $250 million toward orphan site cleanup and technology development/demonstration over a five year period. The federal government has committed an additional $25 million to assess contaminated sites on federal crown land. Over 40 orphan high-risk contaminated sites, over 230 federal sites and over 35 technology development/demonstration projects have been addressed. The Canadian Council of Ministers of the Environment has developed a series of guidance documents to ensure a consistent and successful implementation of the Program. The management/regulation scheme of contaminated sites generally consists of： 1) identifying and investigating sites, 2) determining site contamination, 3) recognizing responsibility and liability, 4) assessing priority for remediation, 5) activation, evaluation and implementing remediation options, and 6) documenting remediation completion. The NCSRP supported the successful development/demonstration of a wide range of innovative remedial technologies. They are related to stabilization/solidification, thermal washing/flushing, advanced oxidation, sonics, and groundwater contaminated with hydrocarbons, PAHS, PCBs, heavy metals, and other hazardous pollutants in a variety of site environments.

• Korean Journal of Ecology and Environment
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• v.42 no.4
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• pp.452-464
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• 2009

The objectives of this study were to evaluate spatial and temporal variability of water quality in 10 reservoirs and identify the key nutrients (N, P) influencing chlorophyll-a (CHL) along with analysis of empirical models and zonal patterns of total phosphorus (TP) and CHL. We analyzed total nitrogen (TN), TP, CHL, water clarity (Secchi depth, SD), and evaluated potential limiting nutrient using ambient N:P ratios and previous criteria of ambient nutrients. Water clarity and CHL varied largely depending on the seasonal monsoon and type of reservoir, but trophic state was diagnosed as eutrophy, base on mean CHL in most reservoirs. The peak of TP did not match the contents of CHL due to rapid flushing during the high run-off period. In the reservoir of DR, regression coefficient in the $P_r$ was 0.510 but was 0.159 in the $M_o$, while the TP-CHL relation in the YR increased during the monsoon compared to the premonsoon. The regression coefficient in the $P_r$ was not statistically significant but the value of $M_o$ was 0.250. TP showed similar longitudinal zonal gradients among the reservoirs of DR, YR and JR. Empirical models of TP-CHL, based on overall data, showed that CHL was determined by phosphorus($R^2=0.244$, p=0.0019). Regression analysis of CHL-SD showed a stronger linear fit ($R^2=0.638$, p<0.001) than the TP-CHL model.

• Journal of Soil and Groundwater Environment
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• v.18 no.6
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• pp.8-17
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• 2013

The contamination of chlorinated ethenes at an industrial complex, Wonju, Korea, was examined based on sixteen rounds of groundwater quality data collected from 2009 to 2013. Remediation technologies such as soil vapor extraction, soil flushing, biostimulation, and pumping-and-treatment have been applied to eliminate the contaminant sources of trichloroethylene (TCE) and to prevent the migration of TCE plume from remediation target zones. At each remediation target zone, temporal monitoring data before and after the application of remediation techniques showed that the aqueous concentrations of TCE plume present at and around the main source areas decreased significantly as a result of remediation technologies. However, the TCE concentration of the plumes at the downstream area remained unchanged in response to the remediation action, but it showed a great fluctuation according to seasonal recharge variation during the monitoring period. Therefore, variations in the contaminant flux across three transects were analyzed. Prior to the remediation action, the concentration and mass discharges of TCE at the transects were affected by seasonal recharge variation and residual DNAPLs sources. After the remediation, the effect of remediation took place clearly at the transects. By tracing a time-series of plume evolution, a greater variation in the TCE concentrations was detected at the plumes near the source zones compared to the relatively stable plumes in the downstream. The difference in the temporal profiles of TCE concentrations between the plumes in the source zone and those in the downstream could have resulted from remedial actions taken at the source zones. This study demonstrates that long term monitoring data are useful in assessing the effectiveness of remediation practices.

• Journal of Korean Society on Water Environment
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• v.21 no.3
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• pp.277-283
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• 2005

This paper aimed to compare the daily water quality as well as the hydrological data gathered for the past two years (2000 to 2001) between the two influent rivers of Paldang reservoir. The analysis also has been carried out to draw out the factors that affect the water quality at the dam site, where the main drinking water drawing point is located. The relationship between total amount of monthly rainfall and monthly inflow showed $r^2=0.74$ (p<0.05). The highest peak of inflow of influent rivers recorded in August and September (in the year of 2000) and July and August (2001). Average inflows of influent rivers in 2000 and 2001 are calculated at 209.0, 161.5 CMS (Bughangang), 268.6, 148.2 CMS(Namhangang), and 7.8, 5.0 CMS (Gyeongancheon). The formula which was driven from the relationship between inflow and COD load of influent rivers, explained that COD concentration in general increased with the inflow. But during the rainy seasons (July, August, and September), COD concentration decreased according to the increase of inflow. The daily rainfall and COD concentration(or load) during the rainy season (August and September in the year of 2000, July and August in 2001) indicated that the peak of COD load correspond with the rainfall, which decreased sharply after 3 or 4 days. The reason was thought that the high COD load was diluted rapidly by the rain flow. Water temperature, pH and conductivity measured at dam site decreased obviously when the inflow sharply increased. Peak period of total phosphorus concentration coincided with that of inflow. In rainy season, chlorophyll-a concentration decreased obviously as the inflow increased. The reason can be ascribed to the flushing effect caused by the operation of floodgate.

• The Korean Journal of Pain
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• v.32 no.3
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• pp.178-186
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• 2019

Background: Epidural steroid injections (ESIs) have been widely used in managing spinal pain. Dexamethasone has recently emerged as a useful drug in this setting, relative to particulate steroids, although the associated systemic effects have not been fully elucidated. This study aimed to investigate the incidences and types of systemic effects after fluoroscopically guided ESI with dexamethasone. Methods: This retrospective study included 888 ESIs with dexamethasone (fluoroscopically guided at the cervical and lumbosacral levels) performed on 825 patients during January to June 2017. Data regarding systemic effects were collected via telephone interviews using a standardized questionnaire at 2 weeks after the procedure. Data on patient demographic, clinical, and procedural characteristics were collected and analyzed to identify factors that were associated with systemic effects. All statistical analyses were performed using the chi-squared test. Results: Among the 825 patients, 40 patients (4.8%) experienced systemic effects during the 2-week follow-up period. The most common systemic effect was facial flushing (12 patients, 1.5%), which was followed by urticaria (7 patients, 0.8%) and insomnia (7 patients, 0.8%). A history of spine surgery was significantly associated with the occurrence of systemic effects (P = 0.036). Systemic effects were significantly more common for injections at the cervical level than at the lumbar level (P = 0.019). Conclusions: Approximately 4.8% of the patients who underwent ESI with dexamethasone experienced minor and transient systemic effects. These effects were more common in patients who had undergone a previous spine surgery or received a cervical ESI.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.28 no.1
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• pp.9-14
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• 2022

Packages of different atmospheres (air (control), 100% CO₂, vacuum, and vacuum + O₂ absorber) were prepared for 0.4 kg rice cake (ddukgukdduk) using gas-barrier plastic film and stored at 10℃ for 11 days. The stored products were evaluated in their packages atmosphere, total aerobic bacteria, yeast and molds, texture and sensory quality during storage period. In the air package, the O₂ concentration decreased from initial 21% to 16% on storage 4 days and the CO₂ concentration increased to 23% on storage of 11 days, which resulted from the growth of microorganisms. CO₂ concentration decreased from initial 98% to 36% on storage 11 days in the 100% CO₂ package. It is reasoned that CO₂ was dissolved into the product reducing the volume of the package. Vacuum and vacuum +O₂ absorber package maintained shrunk vacuum condition until 11 days of storage. Total aerobic bacteria count increased significantly in the control package (6.41 log (cfu/g) after 11 days) compared to the 100% CO₂ package (4.96 log (cfu/g) after 11 days). Yeast and molds were 6.66 in control package, 3.43 in 100% CO₂ package, 4.66 in vacuum package, and 3.78 in vacuum + O₂ absorber package after 11 days. There was no significant difference between control and the other treatments for the texture of the stored products. Sensory quality was the worst in control package on the storage of 8 days. All treatment groups except control improved the quality preservation, but vacuum and vacuum + O₂ absorber packages suffered from cracking of the product. Thus 100% CO₂ flushing is suggested as a desired packaging condition.

• Membrane Journal
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• v.19 no.1
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• pp.34-46
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• 2009

Effect of backflushing on the membrane fouling for polyethylene capillary membranes was examined by measuring the flux of $Al_2O_3$ colloidal suspensions through the cross flow microfiltration. In the comparison of with and without the application of backflushing, the hydraulic resistance to permeate flow of the suspension was less with backfluslng, but the Increasing rate in permeate resistance was higher. Regardless of backflushing, the cake filtration was dominant at the initial period of filtration with backflushing, being followed by the pore blocking. And at steady state, the fouling mechanism is almost governed by the cake filtration model. On the contrary, the pore blocking preceded the cake filtration in the initial stage of the original membrane before backflushing. And irrespective of backflushing, the ratio of cake filtration to total fouling increased, compared with that fur before backflushing. For the membrane with $0.24{\mu}m$ pore size, the permeate resistance was higher than that of $0.34{\mu}m$ pore size membrane. but the ratio of cake filtration was smaller than that of large pore membrane. In comparing the ratio of each fouling component to the total fouling for the case with backflushing pore blocking was 7.8% and cake filtration was 92.2%. for the case without backflushing, total fouling was composed of 9.6% pore blocking and 90.4% cake filtration.

• The Korean Journal of Pain
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• v.4 no.2
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• pp.111-121
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• 1991

It has been well known that the splanchnic nerve block is effective for patients who suffer from intractable upper abdominal pain. However, it is unclear whether the effect of the splanchnic nerve block depends on varied alcoholic concentration. In this study, an attempt was made to use absolute ethanol on patients who recieved a splanchnic nerve block at Severance Hospital during the period from September l990 to April l991. The results are as follows; 1) Among the 33 patients, including 22 males and 1l females, the fifties and sixties were the major age groups. 2) Stomach cancer was the most common underlying disease(13 cases), with pancreatic can- cer next(9 cases). 3) The main locations of pain were the upper abdomen, epigastrium, and entire abdomen in decreasing order. 4) There were 17 cases who had had chemotherapy, and 1l cases of whom had had surgery before the splanchnic nerve block. 5) The volume of alcohol used was 12 ml bilaterally. 6) Among the 33 patients, 15.2% required a second block within two weeks of the first block. One case required a third block. 7) The most common complications of splanchnic nerve block were hypotension(33.3%), occasional transient sharp burning pain, flushing of face, pain on injection site, nausea, vomiting, dyspnea, chest discomfort and diarrhea. 8) The supplemental block most commonly used was a continuous epidural block. It was used both as a diagnostic block and to afford relief from pain before the splanchnic nerve block was done. 9) The interval between the receiving the absolute ethanol block and discharge was within 2 weeks in l5 cases. But, in the patients with poor general health, the interval between the splanchnic nerve block and discharge prolonged. The above results suggest that bilateral splanchnic nerve block done with absolute ethanol after an effective test block with 1% lidocaine under C-arm fluroscopic control is satisfactory and reliable. Still, 26.6% of the patients received a repeat block within 2 weeks. Insufficient spread of ethanol due to its small volume seems to be a major factor in the repeat block. Minimizing the incidence of repeat block remains a problem to be solved.

• Journal of Embryo Transfer
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• v.10 no.1
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• pp.73-82
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• 1995

large scale production of cloned embryos requires the technology of multiple generation nuclear transplantation(NT) using NT embryos as the subsequent donor nuclei. The purposes of this study were producing the second generation cloned rabbit embryos, and also to determine the electrofusion rate and in vitro developmental potential comparatively in the cloned embryos of the first and second NT generation. The embryos of 16-cell stage were collected from the mated does by flushing oviducts with Dulbecco's phosphate buffered saline(D-PBS) containing 10% fetal calf serum(FCS) at 47 hours after hCG injection In the first generation NT, the nuclear donor embryos were synchronized in the phase of Gi /S transition of 32-cell stage. The first generation NT embryos which were developed to 8-cell were synchronized in Gi /S transition phase of the following 16-cell stage and used as donor nuclei for second generation Synchronization of the cell cycle of blastomeres was induced, first, using an inhibitor of microtuble polymerization, colcemid for 10 hours to arrest blastomeres in M phase, and secondly, using a DNA synthesis inhibitor, aphidicolin for 1.5 to 2 hours to arrest them in Gi /S transition boundary. The recipient cytoplasms were obtained by removing the nucleus and the first polar body from the oocytes collected at 14 hours after hCG injection. The separated donor blastomeres were injected into the enucleated recipient oocytes by micromanipulation and were electrofused by electrical stimulation of three pulses for 60 $\mu$sec at 1.25 kV /cm in 0.28 M rnannitol solution The fused oocytes were co-cultured with a monolayer of rabbit oviductal epithelial cells in M-199 solution containing 10% FCS for 120 hours at 39$^{\circ}C$ in a 5% $CO_2$ incubator. Following in vitro culture of the first and second generation cloned embryos to blastocyst stage, they were stained with Hoechst 33342 dye for counting the number of blastomeres by fluorescence microscopy. The results obtained were summarized as follows: 1. The electrofusion rate was found to be similar as 79.4 and 91.5% in the first and second generation NT rabbit embryos, respectively. 2. The in vitro developmental potential to blastocyst stage of the second generation NT embryos (23.3%) was found significantly(p<0.05) lower, compared with that of the first generation NT embryos (56.8%). 3. The mean blastomeres counts of embryos developed to blastosyst stage following in vitro culture for 120 hours and also their daily cell cycles during the culture period were decreased significantly (p<0.05) to 104.3 cells and 1.33 cylces in the second NT generation, compoared with 210.4 cells and 1.54 cycles in the first NT generation, respectively.