In rumen in vitro experiments, although nitrogen gas (N2) flushing has been widely used, its effects on rumen fermentation characteristics are not clearly determined. The present study is the first to evaluate the effects of N2 flushing on rumen fermentation characteristics in in vitro batch culture system by comparing with new applicable non-metabolizable gas: argon (Ar). The rumen fluid was taken from two Korean native heifers followed by incubation for 3, 9, 12, and 24 h with N2 or Ar flushing. As a result, in all incubation time, N2 flushing resulted in higher total gas production than Ar flushing (p < 0.01). Additionally, in N2 flushing group, ammonia nitrogen was increased (p < 0.01). However, volatile fatty acids profiles and pH were not affected by the flushing gases (p > 0.05). In conclusion, the present study demonstrated that N2 flushing can influence the rumen nitrogen metabolism via increased ammonia nitrogen concentration and Ar flushing can be used as a new alternative flushing gas.
Journal of the Korean Society of Marine Environment & Safety
/
v.23
no.5
/
pp.541-548
/
2017
A theoretical study on gas-liquid two-phase flow flushing systemnitrogen gas to the oil used for existing flushing equipment was conducted on the basis of ISO code so as to improve performance of existing high-temperature oil flushing equipment used in ocean plant facility drying field. For study, we analyzed process simulation results mixed fluid mixing ratio, temperature, Reynolds number and liquid hold up affectcleaning performance after designing oil-nitrogen gas mixture flushing system process. As a result, as the volume flow rate of mixed fluid increases with the tube diameter the volume fraction of the gas phase constant, the liquid fraction difference value at the inlet and outlet of horizontal hydraulic piping increases. It was found that the phase distribution between oil and nitrogen gas bubbles varies depending on the position the pipe lengthdirection. This change in phase distribution is expected to have a significant impact on the clean performance of an oil-nitrogen gas mixture flushing system.
The effects of pH, saturated oxygen, and back-flushing media were investigated in hybrid process of tubular ceramic microfiltration and $TiO_2$ photocatalyst-loaded PES (polyethersulfone) beads for advanced drinking water treatment, and compared results of water, nitrogen, or oxygen back-flushing in the viewpoints of membrane fouling resistance ($R_f$), permeate flux (J) and total treated water ($V_T$). $R_f$ decreased, and J and $V_T$ increased as decreasing pH. Turbidity treatment efficiencies were similar at water or nitrogen back-flushing independent of pH, but DOM (dissolved organic matter) treatment efficiency did not have a trend at water back-flushing. $R_f$ at NBF (no back-flushing) with SO (saturated oxygen) was the lower than that at NBF without SO. Also, the DOM treatment efficiency at NBF with SO was the lower than that at NBF without SO. It happened because OH radicals produced by reaction of SO and photocatalyst could dilute with water inside the module. The DOM treatment efficiency of gas back-flushing showed the larger than that of water back-flushing at back-flushig period 10 min. It proved that the adsorption or photo-oxidation of PES beads could be activated by the more effective bead-cleaning of gas back-flushing than water back-flushing.
Shallow coal resources are increasingly depleted, the mining has entered the deep stage. Due to "High stress, high gas, strong adsorption and low permeability" of coal seam, the gas drainage has become more difficult and the probability of coal and gas outburst accident increases. Based on the flow solid coupling theory of coal seam gas, the coupling model about stress and gas seepage of coal seam was set up by solid module and Darcy module in Comsol Multiphysics. The gas extraction effects were researched after applying hydraulic technology to increase permeability. The results showed that the effective influence radius increases with the expanded borehole radius and drainage time, decreases with initial gas pressure. The relationship between the effective influence radius and various factors presents in the form: $y=a+{\frac{b}{\left(1+{(\frac{x}{x_0})^p}\right)}}$. The effective influence radius with multiple boreholes is obviously larger than that of the single hole. According to the actual coal seam and gas geological conditions, appropriate layout way was selected to achieve the best effect. The field application results are consistent with the simulation results. It is found that the horizontal stress plays a very important role in coal seam drainage effect. The stress distribution change around the drilling hole will lead to the changes in porosity of coal seam, further resulting in permeability evolution and finally gas pressure distribution varies.
Proceedings of the Korean Society of Soil and Groundwater Environment Conference
/
2003.04a
/
pp.418-421
/
2003
Surfactant enhanced in-situ soil flushing was peformed to remediate the soil and groundwater at an oil contaminated site, and the effluent solution was treated by the chemical treatment process including DAF(Dissolved Air Flotation). A section from the contaminated site(4.5m$\times$4.5m$\times$6.0m) was selected for the research, which was composed of heterogeneous sandy and silt-sandy soils with average Hydraulic conductivity of 2.0$\times$10$^{-4}$ cm/sec. Two percent of sorbitan monooleate(POE 20) and 0.07% of iso-prophyl alcohol were mixed for the surfactant solution and 3 pore volumes of surfactant solution were injected to remove oil from the contaminant section. Four injection wells and two extraction wells were built in the section to flush surfactant solution. Water samples taken from extraction wells and the storage tank were analyzed by GC(gas-chromatography) for TPH concentration with different time. Five pore volumes of solution were extracted while TPH concentration in soil and groundwater at the section were below the Waste Water Discharge Limit(WWDL). Total 18.5kg of oil (TPH) was removed from the section. The concentration of heavy metals in the effluent solution also increased with the increase of TPH concentration, suggesting that the surfactant enhanced in-situ flushing be available to remove not only oil but heavy metals from contaminated sites. Results suggest that in-situ soil flushing and chemical treatment process including DAF could be a successful process to remediate contaminated sites distributed in Korea.
Lee, Hye Lim;An, Duck Soon;Jung, Yong Bae;Lee, Dong Sun
KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
/
v.22
no.3
/
pp.79-84
/
2016
A kimchi container actively controlling $CO_2$ concentration by timely flushing of $CO_2$ gas was structured and tested in its capability and effectiveness because high $CO_2$ concentration enhances the sensory flavor of the product. The inlet and outlet valves of $CO_2$ gas were programmed to open and close allowing synchronous vent/$CO_2$ flush according to the requirements of its dissolution in the contained kimchi. During the chilled storage, the headspace of container could be maintained at desired high $CO_2$ concentration providing the preferred kimchi in sensory quality compared to control of the conventional container. However, there was no significant difference between the high $CO_2$ container and control (container simply closed with air) in kimchi quality attributes of pH, titratable acidity, total viable bacterial count, Lactobacillus sp. count and Leuconostoc sp. count. The flow rate and time interval of $CO_2$ flushing need to be adjusted considering the kimchi amount, headspace volume and ripening time. The designed system has potential to be applied in refrigerator appliances in homes and food service industry.
The risk of cross-contamination in dental clinic is very high. Those who are engaged in dental clinic are exposed to various microorganisms in saliva and blood of patient. Potential possibility of cross-contamination of patient to patient, patient to dentist, dentist to laboratory technician always exist, which is important in the view of public health. It is well known that microorganisms may cause cross-contamination by suck-back of microorganisms into the water supply line or air supply line of dental unit and sprayed back into the next patient's oral cavity. The majority of microorganisms coming from dental unit are water microorganisms from the main water supply which have colonized the tube within the units and multiplied in the relatively warm and stagnant conditions. The purpose of this study is to measure the extent of microbial contamination of dental unit and ultrasonic scaler, to evaluate that dental unit water supply is suitable for drinking water, and to assess the effect of flushing on reduction of microbial contamination of dental unit and ultrasonic scaler. In the first experiment, water samples(50ml) from 20 dental units and 10 ultrasonic scalers in Seoul National Univ. Hosp. were tested for the presence of coliform. The samples were filtered by membrane filtration technique.(Microfil system, Millipore Co. U. S. A.) The filter was then placed onto MacConkey agar plate and the plates with filter on it were incubated aerobically at $37^{\circ}C$ for 5 days. The colors and shapes of colonies were examined if those were coliform. To verify the presence of coliform, the colonies were inoculated into phenol red lactose broth and incubated aerobically at $37^{\circ}C$ for 2 days. The fomation of gas was observed. In the second experiment, water samples from 20 handpieces, 10 ultrasonic scalers and 30 A/W syringes after 0, 2, 4, 6 min. flushing respectively were taken. $200{\mu}l$ water samples were spreaded on Brain Heart Infusion agar plate and the plates were incubated aerobically at $37^{\circ}C$ for 5 days. The number of colony was counted. The results obtained were summarized as follows 1. The water from dental unit and ultrasonic scaler was not suitable for drinking water. 2. No coliform was founded in dental unit and ultrasonic scaler water supply. 3. The number of colony of dental unit and ultrasonic scaler was highest in the group of o min. flushing(p<0.05). 4. There was no statistically significant difference in the extent of microbial contamination among handpiece, ultrasonic scaler and A/W syringe (p>0.05). 5. The number of colony was lowest in the group of 4 min. flushing, but there was no statistically significant difference among 2, 4, 6 min. flushing groups.(p>0.05) 6. It is recommended to flush dental unit water line for 4 min. after use on each patient.
Surfactant enhanced in-situ soil flushing was performed to remediate the soil and groundwater at an oil contaminated site, where had been used as a military vehicle repair area for 40 years. A section from the contaminated site (4.5 m $\times$ 4.5 m $\times$ 6.0 m) was selected for the research, which was composed of heterogeneous sandy and silt-sandy soils with average $K_d$ of 2.0$\times$$10^{-4}$cm/sec. Two percent of sorbitan monooleate (POE 20) and 0.07% of iso-prophyl alcohol were mixed for the surfactant solution and 3 pore volumes of surfactant solution were injected to remove oil from the contaminated section. Four injection wells and two extraction wells were built in the section to flush surfactant solution. Water samples taken from extraction wells and the storage tank were analyzed on a gas-chromatography (GC) for TPH concentration in the effluent with different time. Five pore volumes of solution were extracted while TPH concentration in soil and groundwater at the section were below the Waste Water Discharge Limit (WWDL). The effluent TPH concentration from wells with only water flushing was below 10 ppm. However, the effluent concentration using surfactant solution flushing increased to 1751 ppm, which was more than 170 times compared with the concentration with only water flushing. Total 18.5 kg of oil (TPH) was removed from the soil and groundwater at the section. The concentration of heavy metals in the effluent solution also increased with the increase of TPH concentration, suggesting that the surfactant enhanced in-situ flushing be available to remove not only oil but heavy metals from contaminated sites. The removal efficiency of surfactant enhanced in-situ flushing was investigated at the real contaminated site in Korea. Results suggest that in-situ soil flushing could be a successful process to remediate contaminated sites distributed in Korea.
Active air flushing mini-pumps were installed in a rigid polypropylene container ($32cm{\time}23cm{\time}18cm$) containing 900 g of fresh green peppers for effectively controlling its $O_2$ concentration on real time basis to preserve the product quality. The performance of the constructed system was compared to that of the modified atmosphere (MA) container system with gas diffusion tube controlled in close/open cycles responding to real time $O_2$ concentration at 10 and $20^{\circ}C$. In the control logic, the $O_2$ concentration was programmed to be located exactly at 13% or stay in the range of 13-15%. The active air flushing system could control the $O_2$ concentration in the desired level or range at both temperatures, while the passive diffusion system could work only under the low temperature condition of $10^{\circ}C$. At higher temperature of $20^{\circ}C$, the passive diffusion system could not manage the produce respiration increased more highly than the gas transfer through the diffusion tube, resulting in too low $O_2$ concentration and too high $CO_2$ concentration which would be injurious to the green pepper. When tested at $20^{\circ}C$, the MA container system could preserve the green pepper better than the perforated air package in terms of weight loss, ascorbic acid and chlorophyll contents and firmness.
Journal of the Korean Society of Hazard Mitigation
/
v.1
no.3
s.3
/
pp.83-92
/
2001
Field investigations for subsurface soil and groundwater at a gas station showed that the site was severely contaminated and even petroleum compounds as free liquid state were observed. Pilot-scale soil flushing and soil vapor extraction process(SVE) were applied to evaluate the effectiveness of pollutants removal. Surfactant solution, Tween 80, was used to enhance the solubility of petroleum compounds and resulted in about 10 times increase on TPH(Total Petroleum Hydrocarbon) concentration. As for SVE method, maximum concentration of TPH and BTEX reached within 24 hours of extraction and then continuously decreased. Considerations on the groundwater level and the kinetic limitation for volatilization of contaminants have to be taken into account for the effective application of SVE process.
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