• Title/Summary/Keyword: Fluorescent sensors

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Molecular System Design for the Acetylcholine Fluorescent Chemosensor

  • Kah, Kwang-Nak
    • Journal of Sensor Science and Technology
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    • v.6 no.6
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    • pp.508-513
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    • 1997
  • We exploited a new molecular system - acetylcholine (neurotransmitter) detection system as a building block for the perfect molecular information system (sensing membrane of the chemical sensor) - using water soluble calix[n]arene-p-sulfonates which are useful even in aqueous (water/methanol) neutral solution. This achievement is due to several outstanding properties of these calix[n]arene derivatives such as low $pK_{a}$ values, cation-interactions, and high water-solubility, etc.

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Design and Synthesis of Metallopeptide Sensors: Tuning Selectivity with Ligand Variation

  • Kim, Joung-Min;Joshi, Bishnu Prasad;Lee, Keun-Hyeung
    • Bulletin of the Korean Chemical Society
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    • v.31 no.9
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    • pp.2537-2541
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    • 2010
  • We chose a fluorescent pentapeptide sensor (-CPGHE) containing a dansyl fluorophore as a model peptide and investigated whether the selectivity and sensitivity of the peptides for heavy and transition metal ions could be tuned by changing amino acid sequence. In this process, we developed a selective peptide sensor, Cp1-d (-HHPGE, $K_d\;=\;670\;nM$) for detection of $Zn^{2+}$ in 100% aqueous solution and a selective and sensitive peptide sensor, Cp1-e (-CCHPGE, $K_d\;=\;24\;nM$) for detection of $Cd^{2+}$ in 100% aqueous solution. Overall results indicate that the selectivity and sensitivity of the metallopeptide sensors to specific heavy and transition metal ions can be tuned by changing amino acid sequence.

Energy and Safety Efficient LED Street-light Control System Based on Ubiquitous Sensor Network (USN 기반의 에너지 및 안전성 효율적인 LED 가로등 제어 시스템)

  • Cho, Myeon-gyun;Kim, Shik;Yang, Woo Suk
    • IEMEK Journal of Embedded Systems and Applications
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    • v.6 no.1
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    • pp.25-31
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    • 2011
  • LEDs are becoming the most suitable candidate replacing traditional fluorescent street-light lamps because of its energy efficiency and high brightness. Furthermore, most countries are urging to pursue energy savings in conjunction with IT and sensor network. In order to conserve energy of LED lamp and ensure the safety of pedestrian, we propose a new smart control method for LED light system based on USN using compound sensors, such as illuminance, motion, temperature and humidity sensor. An elaborate simulation shows that the proposed system with a smart control based switching can reduce the energy by 40%, compared to the previous street-light system with a fixed time based switching.

Synthesis and Characterization of 2, 6-Di-(4'-Methyl Phenyl) Pyrylium Fluoroborate and Perchlorate in Single Step Salts Using 4'-Methyl Acetophenone

  • Wie, Jin-Hyeong;Hong, Young-Min;Kim, Hyun-Ook;Kim, Kyung-Hoon;Cho, Sung-Il
    • Journal of Sensor Science and Technology
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    • v.21 no.1
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    • pp.13-20
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    • 2012
  • Due to its high conductivity, pyrylium has been frequently used in electron transfer reactions or in the synthesis of various organic materials. It has also been used as a sensor material. Traditionally, the compounds have been synthesized using various methods; mostly in a multiple steps. In this study, two pyrylium salts, 2, 6-di-(4'-methylphenyl) pyrylium fluoroborate and perchlorate were synthesized. The synthesis of these products was confirmed by 1H-NMR, LC/TOF-MS and FT-IR analyses while their photo-properties were analyzed using UV/VIS spectrophotometry. In addition, the electron transfer capacities of the salts were analyzed with a conductivity meter, it was found that their electron conductivities were high. When the synthesized compounds were dissolved in acetone, a green fluorescent material was observed to form. The fluorescent material can be used as a sensitizer in the electrical industry.

Microfluidic immunoassay using superparamagnetic nanoparticles in an enhanced magnetic field gradient (강화된 자기장 구배 하에서 나노자성입자를 이용한 미세유체 기반의 면역 측정)

  • Hahn, Young-Ki;Kang, Joo-H.;Kim, Kyu-Sung;Park, Je-Kyun
    • Journal of Sensor Science and Technology
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    • v.15 no.3
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    • pp.158-163
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    • 2006
  • This paper reports a novel immunoassay method using superparamagnetic nanoparticles and an enhanced magnetic field gradient for the detection of protein in a microfluidic device. We use superparamagnetic nanoparticles as a label and fluorescent polystyrene beads as a solid support. Based on this platform, magnetic force-based microfluidic immunoassay is successfully applied to analyze the concentration of IgG as model analytes. In addition, we present ferromagnetic microstructure connected with a permanent magnet to increase magnetic flux density gradient (dB/dx, ${\sim}10^{4}$ T/m), which makes limit of detection reduced. The detection limit is reduced to about 1 pg/mL.

Ultra-thin Film Assembly of a Novel Biomaterial Containing Protein and Functionalized Polymer for Sensor Application

  • Lim, Jeong-Ok;Sohn, Byung-Ki;Huh, Jeung-Soo
    • Journal of Sensor Science and Technology
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    • v.4 no.4
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    • pp.81-87
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    • 1995
  • A novel biomaterial capable of incorporating biotinylated biomolecule has been synthesized. Our strategy is to biotinylate one-dimensional electroactive polymers and use a bridging streptavidin protein on Langmuir-Blodgett (LB) organized films. These copolymers are derivatized with long alkyl chains and biotin moieties to bind, respectively, to the hydrophobic surface and the biotinylated species, through the biotin and streptavidin complexation. We utilize the polymer assembly approach to attach a signal transducing biomolecule biotinylated phycoerythrin (B-PE) into this novel biomaterial by binding the unoccupied biotin binding sites on the bound streptavidin (4 sites total). The pressure-area isotherm of the protein injected monolayer showed area expansion. A characteristic fluorescent emission peak at 576nm was detected from the monolayer transferred onto a solid substrate. These observations demonstrated the promise of the organized thin polymer assemblies for their application to the sensor system.

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Reducing the Effects of Noise Light in a Visible Light Communication System Using Two Color LEDs (가시광통신 시스템에서 2색 LED를 이용한 잡음광의 영향 감소)

  • Lee, Seong-Ho
    • Journal of Sensor Science and Technology
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    • v.21 no.6
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    • pp.429-433
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    • 2012
  • In this paper, we reduced the optical noise interference in a visible light communication system using two color LEDs. In the transmitter, the original and the inverted signals of the transmitted data modulated a red LED and a blue LED, respectively. In the receiver, a differential detector which is composed of two photodetectors and an optical red filter detected the mixed signal radiated from the two LEDs. In an environment that the optical noise from a fluorescent lamp exists, the signal-to-noise ratio in this system was improved by about 20dB compared to that in the conventional system which uses a single LED and a single photodetector.

Minireview on Nuclear Spin Polarization in Optically-Pumped Diamond Nitrogen Vacancy Centers

  • Jeong, Keunhong
    • Journal of the Korean Magnetic Resonance Society
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    • v.20 no.4
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    • pp.114-120
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    • 2016
  • Nitrogen vacancy-centered diamond has recently emerged as a promising material for various applications due to its special optical and magnetic properties. In particular, its applications as a fluorescent biomarker with small toxicity, magnetic field and electric field sensors have been a topic of great interest. Recent review (R. Schirhagl et al 2014) introduced those applications using single NV-center in nanodiamond. In this minireview, I introduce the rapidly emerging DNP (Dynamic Nuclear Polarization) field using optically-pumped NV center in diamonds. Additionally, the possibility of exploiting the optically-pumped NV center for polarization transfer source, which will produce a profound impact on room temperature DNP, will be discussed.

Synthesis and Fluorescence Properties of New Rhodamine 6G Derivarives Containing Hydroxy Coumarin Moiety (새로운 로다민 6G 하이드록시 쿠마린 유도체의 합성과 형광특성)

  • Park, Seong Ho;Chang, Seung Hyun
    • Journal of Environmental Science International
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    • v.25 no.9
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    • pp.1283-1288
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    • 2016
  • In this study, we synthesized fluorescent sensors from rhodamine 6G derivatives and hydroxy coumarin. The synthetic routes to the rhodamine 6G derivatives containing hydroxy coumarin are shown in Fig. 1. Two derivatives were synthesized through Schiff base reactions. The structures of the new compounds were confirmed by melting point, $^1H$-NMR, and GC-MS analyses. The compounds were found to selectively bind to tin ($Sn^{2+}$) ion by fluorescence titration using various metal cations. Longer carbon chains gave more sensitivity. $Sn^{2+}$ ions exhibited the strongest fluorescence among the nime ions. The binding analysis using Job plots suggested that compounds form 1:1 complexes with the $Sn^{2+}$ ions.

Research on the Indices for Demonstrating Cell Conditions

  • Kim, Ik-Hyun;Pan, Sung-Bum
    • Journal of Sensor Science and Technology
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    • v.21 no.5
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    • pp.324-328
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    • 2012
  • In the past a few decades, various kinds of cells have been examined in laboratories all over the world, and their interesting results have been expressed through various methods in journal publications. For a representative example, the increment or reduction of cell numbers during a bio-related experimental process has been demonstrated using the hazard ratio in survival analysis or in the form of a graph. In addition, the condition of cells such as their normality or abnormality would be indicated by the images of the cell nuclei or membranes treated with proper fluorescent labeling. However, the above methods seem to not be quantitative but rather qualitative assessments, which might be difficult to provide people with the eidetic understanding through parameters or numerical data. With adequate suggestions on any indices enabling the explanation for cell conditions, some analyses may be underestimated due to the lack of objectiveness caused by merely linguistic evaluation for the cell conditions, not numerally scientific interpretation. Therefore, in this study, we would suggest some indices enabling quantitative analysis on the cellular conditions.