• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.10a
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• pp.106-106
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• 2003

• Journal of Environmental Health Sciences
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• v.5 no.1
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• pp.40-45
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• 1978

We have selected 36 schools of a total of night high schools for boys and girls in seoul and measured intensity of illumination of the classroom, The corridors and the stairs that students study and live, with priority given to an illumination, a primary factor of environmental sanitation of school following is the result. 1. The maximum average intensity of illumination of the classroom is 93.2 Lux, and the minimum average intensity of illumination 39.5 Lux. Mean$\pm$S, is 59.03$\pm$22.8 Lux 2. The maximum average intensity of illumination of the corridor is 39.2 Lux, and the minimum average intensity of illumination 11.1 Lux. 3. The maximum average intensity of illumination of the stair is 11.58 Lux, and the minimum average intensity, of illumination 4.92 Lux, mean$\pm$S.D is 7.88$\pm$10.0Lux. 4. Schools with tile illumination facilities more than 50 Lux are 63.8% and less than 50 Lux are 36.2%. 5. Schools with 9-11 facilities of a source of light per classroom by a fluorescent lamp are the most as 30.50%. 6. As for the corridor, schools with the illumination equipment less than 10 Lux are 27.8%, are more than 10 Lux 72.2% 7. As for the stairs schools with the illumination equipment less than 10 Lux are 77.8%, and more than 10 Lux 10 Lux 22.2%.

• 한국가시화정보학회:학술대회논문집
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• 2004.11a
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• pp.86-89
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• 2004

One most feasible way to measure the concentration field in the micro-channel is using micro-LIF(Laser Induced Fluorescence) method. However, an accurate concentration field at a given cross plane in a micro-channel has not been successfully achieved so far due to various limitations in the light illumination and fluorescence signal detection. The present study demonstrates a novel method to provide an ultra thin laser sheet beam having five(5) microns thickness by use of a micro focus laser line generator. The laser sheet beam illuminates an exact plane of concentration measurement field to increase the signal to noise ratio and considerably reduce the depth uncertainty. Nile Blue A was used as fluorescent dye for the present LIF measurement. The enhancement of the fluorescent intensity signals was performed by a solvent mixture of water $(95\%)$ and ethanol (EtOH)/methanol (MeOH) $(5\%)$ mixture. To reduce the rms errors resulted from the CCD electronic noise and other sources, an expansion of grid size was attempted from $1\times1$ to 3(3 or 5(5 pixel data windows and the pertinent signal-to-noise level has been noticeably increased accordingly.

• Analytical Science and Technology
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• v.24 no.3
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• pp.231-235
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• 2011

A spectrofluorimetric method for the determination of EDTA in real samples such as mayonnaise, powder detergent and cleansing cream with tiron (4,5-dihydroxy-1,3-benzenedisulfonic acid) as a fluorimetric reporter was developed. When tiron is chelated with Cu(II), the fluorescent intensity is decreased by a quenching effect. However, when Cu(II)-tiron chelate reacts with EDTA, fluorescent intensity is increased as tiron is released. Several experimental conditions such as pH of the sample solution, the amount of Cu(II), the amount of tiron, heating temperature and heating time were optimized. Fe(III) interfered more seriously than any other ions, interference of Fe(III) could be disregarded, because Fe(III) was scarcely contained in selected real samples. The linear range of EDTA was from $8.0{\times}106{-8}\;M$ to $2.0{\times}10^{-6}\;M$. With this proposed method, the detection limit of Fe(III) was $5.2{\times}10^{-8}\;M$. Recovery yields of 92.7~99.3% were obtained. Based on experimental results, it is proposed that this technique can be applied to the practical determination of EDTA.

• Applied Chemistry for Engineering
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• v.7 no.2
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• pp.252-260
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• 1996

In this article, we investigate into the structural changes of liposome to design its functional membranes by the synthesis of two types of liposomes, DPPC liposome and DTAB of hydrocarbon substance/DPPC liposome. The changes of membrane structures are evaluated by the CF fluorescent intensity measured above and below the phase transition temperature of the membrane, $t_c=41^{\circ}C$. CF fluorescent intensities are enhanced by the CF leakage from DPPC liposome at $45^{\circ}C$, while no changes are observed at $20^{\circ}C$. Under the same conditions, it is observed that the intensity enhanced by CF leakage from DPPC/DTAB liposome is larger than that of DPPC liposome alone, which suggests that DPPC/DTAB liposome has irregular arrangement. Under the presence of $Ca^{2+}$, Quin 2 fluorescent intensity in either DPPC liposome or DPPC/DTAB liposome is significantly increasing at $45^{\circ}C$, while almost none of the changes are observed at $20^{\circ}C$. The fluorescent intensity of DPPC liposome turns out to be larger than that of DPPC/DTAB liposome, which suggests that the DPPC/DTAB liposome is structurally more stable than the DPPC liposome. Additionally, when the analysis is done to observe changes in the shapes of membrane surfaces with ANS fluorescent, ANS fluorescent under DPPC or DPPC/DTAB liposome shows each of different appearances at $45^{\circ}C$ and $20^{\circ}C$ respectively. This result indicates that its respective membrane fluidity is changing above and below of the designated temperatures in phase transition. As to the magnitude of change of its membrane fluidity, DPPC liposome is much larger than DPPC/DTAB liposome. As far as the temperature in phase transition measured by DSC are concerned, it is $41^{\circ}C$ and $32^{\circ}C$ for DPPC and DPPC/DTAB liposome respectively, which suggests that DPPC/DTAB liposome has an irregular molecular arrangement in its structure. That is, it is summed up that DPPC/DTAB turns out to be structurally stable, even so, its structure is irregularly arranged.

• Korean Journal of Microbiology
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• v.47 no.1
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• pp.14-21
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• 2011

Riboflavin synthase catalyzes the formation of one molecule of each riboflavin and 5-amino-6-ribitylamino-2,4-pyrimidinedione by the transfer of a 4-carbon moiety between two molecules of the substrates, 6,7-dimetyl-8-ribityllumazine. The most remarkable feature is the sequence similarity between the N-terminal half (1-97) and the C-terminal half domain (99-213). To investigate the structure and fluorescent characteristics of the N-terminal half of riboflavin synthase (N-RS) in Escherichia coli, more than 10 mutant genes coding for the mutated N-terminal domain of riboflavin synthase were generated by polymerase chain reaction. The genes coding for the proteins were inserted into pQE vector designed for easy purification of protein by 6X-His tagging system, expressed, and the proteins were purified. Almost all mutated N-terminal domain of riboflavin synthases bind to 6,7-dimethyl-8-ribityllumazine and riboflavin as fluorescent ligands. However, N-RS C47D and N-RS ET66,67DQ mutant proteins show colorless, indicating that fluorescent ligands were dissociated during purification. In addition, most mutated proteins show low fluorescent intensity comparing to N-RS wild type, whereas N-RS C48S posses stronger fluorescent intensity than that of wild type protein. Based on this result, N-RS C48S can be used as the tool for high throughput screening system for searching for the compound with inhibitory effect for the riboflavin synthase.

• Journal of Biomedical Engineering Research
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• v.36 no.1
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• pp.16-21
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• 2015

Indocyanine green(ICG) and 5-aminolevulinic acid(5-ALA) have been widely used to mark blood vessels or tumors. However, fluorescent dye detection systems were designed to use one type of dyes only. In this study, we proposed a detection system capable of detecting Indocyanine green and 5-aminolevulinic acid. Multiple filters and light sources are integrated into a single system. In this study, we performed analysis of fluorescent dyes and configured a detection system. During the analysis, it was found that Indocyanine green and 5-aminolevulinic acid have the maximum intensity at $40{\mu}M$. We designed light source for fluorescent dyes and conducted compatibility test using a commercial surgical microscope. The fluorescent dye detection system was configured based on the experimental results. The developed system successfully detects Indocyanine green and 5-aminolevulinic acid. Therefore, more efficient surgical operations can be achieved using both fluorescent dyes at the same time. We expect that the developed system can increase the survival rate of patients.

• Journal of Pharmaceutical Investigation
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• v.18 no.3
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• pp.113-123
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• 1988

A series of water-soluble polyparacyclophanes bearing two diphenylmethane or two diphenyl ether skeletons were investigated to develop useful host compounds by using 1-anilinonaphthalene-6-sulfonate (ANS) and 2-p-toluidinylnaphthalene-6-sulfonate (TNS) as fluorescent hydrophobic substrates in aqueous solution. It was noteworthy that remarkable fluorescent enhancements and blue shifts of ANS and TNS were observed only in the presence of 1,6,20,25-tetraaza[6.1.6.1] paracyclophane (CPM 44) and 1,6,21,27-tetraaza [7.1.7.1] paracyclophane (CPM 55) for diphenylmethane skeleton, and 1,7,21,27-tetraaza-14,34-dioxa [7.1.7.1] paracyclophane (CPE 55) and 1,8,22,29-tetraaza-15,36-dioxa [8.1.8.1] paracyclophane (CPE 66) for diphenyl ether skeleton, comparing with ${\alpha}-\;and\;{\beta}-cyclodextrins$. However, their acyclic analogues such as 4,4'-dimethylaminodiphenylmethane and 4,4'-dimethylaminodiphenyl ether, and paracyclophanes whose cavities were smaller showed only small effects under the same conditions. These facts suggested that hosts and substrates were in an intimate contact which would not occur without larger structures, and thus that guest molecules were strongly incorporated in the hydrophobic cavities of these larger paracyclophanes. The effects of pH on the fluorescent intensity of ANS-CPM 44, ANS-CPM 55, ANS-CPE 55, ANS-CPE 66, TNS-CPM 44, TNS-CPM 55, TNS-CPE 55 and TNS-CPE 66 systems were not significant below pH 2.0, but their fluorescent intensities were markedly reduced with increasing ionic strength.

• Korean Journal of Organic Agriculture
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• v.23 no.3
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• pp.549-565
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• 2015

This study was conducted to investigate the growth characteristics and functional materials of baby vegetables as affected by different LEDs and luminous intensity at Anseongsi, Gyeonggi Province, in 2014. Test crops were beet, chicory, spinach, red leaf lettuce, crown daisy and red mustard purchased from the seed company of Dongbu Hannong and Jinheung. Growth characteristics were measured and the content of functional materials was analyzed 40 days after seeding at plug plate. Treatment of Red+Blue (4:1) at $150{\mu}mol\;m^{-2}s^{-1}$ luminous intensity showed the highest number of leaves in five baby vegetables of beet, chicory, red leaf lettuce, crown daisy and red mustard. The highest shoot length of chicory, spinach, red leaf lettuce, crown daisy and red mustard was obtained from the treatment of Red+Blue (4:1) at $150{\mu}mol\;m^{-2}s^{-1}$ luminous intensity. Fresh weight and dry weight of all six baby vegetables were the highest in treatment of Red+Blue (4:1) at $150{\mu}mol\;m^{-2}s^{-1}$ luminous intensity. Content of chlorophyll a and chlorophyll b of spinach, red leaf lettuce and red mustard showed the highest in Fluorescent lamp at $150{\mu}mol\;m^{-2}s^{-1}$ luminous intensity whereas other crops did not show definite trend under different LEDs lights and luminous intensity. The highest total content of anthocyanins and polyphenol were obtained from the treatment of Red+Blue (4:1) at $150{\mu}mol\;m^{-2}s^{-1}$ luminous intensity in all six baby vegetables. Free radical scavenging activity was highest in all six vegetable crops at $150{\mu}mol\;m^{-2}s^{-1}$ luminous intensity, but it was not different significantly between LEDs. As a result, the growth and the content of functional material of baby vegetables are generally to be increased in Red+Blue (4:1) at $150{\mu}mol\;m^{-2}s^{-1}$ luminous intensity. Mixed light of Red+Blue is thought to give good effect on the growth and the content of functional material in baby vegetable crops. Because there are many differences in regard of LED lights, crop varieties, cultivation and experimental methods in their impact on the growth and functional materials of baby vegetables among researchers, it is considered that a more precise studies are needed for the crop responses to LED light and luminous intensity.

• Bulletin of the Korean Chemical Society
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• v.34 no.7
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• pp.1985-1989
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• 2013

A simple colorimetric and fluorescent anion sensor 1 based on salicylimine showed a high selectivity and sensitivity for detection of cyanide in aqueous solution. The receptor 1 showed high selectivity toward $CN^-$ ions in a 1:1 stoichiometric manner, which induces a fast color change from colorless to orange and a dramatic enhancement in fluorescence intensity selectively for cyanide anions over other anions. Such selectivity resulted from the nucleophilic addition of $CN^-$ to the carbon atom of an electron-deficient imine group. The sensitivity of the fluorescence-based assay (0.06 ${\mu}M$) is below the 1.9 ${\mu}M$ suggested by the World Health Organization (WHO) as the maximum allowable cyanide concentration in drinking water, capable of being a practical system for the monitoring of $CN^-$ concentrations in aqueous samples.