• Title/Summary/Keyword: Fluorescent intensity

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Theoretical Design for the Production of Quinoa (Chenopodium quinoa Willd.) in a Closed Plant Factory

  • Bae, Jong Hyang;Austin, Jirapa;Jeon, Yoon-A;Cha, Mi-Kyung;Cho, Young-Yeol
    • Horticultural Science & Technology
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    • v.34 no.6
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    • pp.840-844
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    • 2016
  • Quinoa (Chenopodium quinoa Willd.) is a grain crop with high nutritional value. The leaves and sprouts of quinoa can also be consumed either raw or cooked, providing considerably nutritional value as well as high antioxidant and anticancer activities. This study was carried out to obtain basic data to assist in the practical design of a plant factory with artificial lighting for the cultivation of quinoa as a leafy vegetable. We estimated the energy content of the quinoa and the electrical energy required to produce this crop. The yield was 1,000 plants per day, with a planting density and light intensity of $0.015m^2$ ($15{\times}10cm$) and $200{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, respectively. The total number of plants, cultivation area, and electricity consumption were estimated to be 25,000, $375m^2$, and $93,750{\mu}mol{\cdot}s^{-1}$, respectively. White fluorescent lamps were used at a power of 20.4 kW from 1,857 fluorescent lamps (FL, 55 W), and the cost for electricity was approximately 1,820 dollars (exchange rate of $1 = 1,200 won) per month. For a daily harvest of 1,000 plants per day in a closed plant factory, the estimated light installation cost, total installation cost, and total production cost would be 15,473, 46,421, and 55,704 dollars, respectively. The calculated production cost per plant, including labor costs, would be 27 cents for the 25-day cultivation period, with a marketable ratio of 80%. Considering the annual total expenses, income, and depreciation costs, the selling price per plant was estimated to be approximately 56 cents.

Effects of the Spectral Quality and Intensity of Light-Emitting Diodes on Growth and Biochemical Composition of Chlorella vulgaris (발광다이오드 광량 및 파장에 따른 Chlorella vulgaris의 생장 및 생화학적 조성 변화 연구)

  • Ji Seung Han;Peijin Li;Tae-Jin Choi;Seok Jin Oh
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.56 no.6
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    • pp.878-888
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    • 2023
  • Growth responses of Chlorella vulgaris exposed to different light intensities and wavelengths of light-emitting diodes (LEDs) were investigated. C. vulgaris was cultured under red LED (650 nm), blue LED (450 nm), green LED (520 nm), and fluorescent lamps (three wavelengths, control). The maximum growth rates (µmax) of C. vulgaris were highest under the blue LED, followed by the red LED, green LED, and fluorescent lamps. The low compensation photon flux density (I0) and low half-saturation constants (Ks) were observed in C. vulgaris cultured under the red LED, indicating that high C. vulgaris growth is closely related to the low light intensity of the red LED suggesting that the red LED can be useful for the biomass production of C. vulgaris. Furthermore, it was observed that under the blue LED during the stationary phase, there was an increase in useful bioactive substances, such as proteins and lipids, which are beneficial for biomass production. In conclusion, the red LED is an economical light source that can enhance cell density, and the blue LED is effective in promoting valuable intracellular substances.

Comparison of Conditions for Cell Death-Inducing Agents Using a High Throughput-Compatible Nuclear Staining Assay (핵 염색을 이용한 세포사멸 유도물질 스크리닝의 조건 비교)

  • Lee, Sang-Han
    • Journal of Life Science
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    • v.18 no.9
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    • pp.1312-1315
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    • 2008
  • High throughput-drug screening plays a pivotal role for early stage of drug discovery process. In the course of assay development for screening of cell death-inducing agents, a protocol that is simple, time-saving, and high throughput-compatible was designed which was confirmed that the protocol can be worked by a HTS-compatible machine. In 96-well format, PC-3 cancer cells (1${\times}10^{4}$ cells/ml) were cultured for 24 hr. After 24 h-incubation with various medicinal plants extracts, the cells were then stained with DAPI for 30 min. The fluorescence intensity of the stained cells was measured semi-automatically with a multilabel counter. To test whether the present assay system effectively works, we screened about 850 medicinal plant extracts, and selected 1 positive crude extracts that contained cell death-inducing activity. These results suggest that the protocol is highly amenable to HTS implementation for a cell death-inducing agent(s).

Quantitative Screening of Insect Cell Transformants Stably Expressing $GFP_{uv}-{\beta}1$, 3-N-acetylglucosaminyltransferase 2 Fusion Protein

  • Deo Vipin Kumar;Kato Tatsuya;Asari Naoko;Park Enoch Y.
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.10 no.3
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    • pp.275-279
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    • 2005
  • Insect cell transformants, stably expressing human $GFP_{uv}-{\beta}1$, 3-N-acetylglucosaminyltransferase 2 $({\beta}3GnT2)$ as the green fluorescent protein $(GFP_{uv})-fused$ protein, were efficiently isolated on Western blot by the quantification of the densitometric intensity of the fusion protein. From almost 150 transformants containing the fusion gene linked to three different types of signal sequence, two transformants, Tn-pXme4a and -pX28a, were successfully selected, showing 8.3 and 8.6 mU/mL ${\beta}3GnT$ activity, respectively. This method requires a screening time almost one-half that required in the isolation of stably transformed cells with high expression levels, and at the same time allows the handling a large number of transformants.

A Study on the Charateristics of ORMOSIL as a Dye Host by Measurement of UV/VIS Spectrophotometer (UV-VIS Spectrophotometer를 이용한 ORMOSIL의 투광특성)

  • Kim, Goo-Dae;Lee, Dong-A;Kang, Hyun-Gyu;Moon, Ji-Woong
    • Analytical Science and Technology
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    • v.7 no.1
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    • pp.17-23
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    • 1994
  • Through sol-gel process, inorganic/organic composite as a dye host was made. It was observed that this composite has about 90% of trnasmittance by UV/VIS spectrophotometer. The transmittance is the most important requirement to decide whether the composite could apply or not as the dye host. The transmittance was increased with decreasing HCl as a catalyst, aging time, and amount of PDMS. Also, fluorescent and transmittant charateristics of Rhodamine 6G Perchlorate that was doped into ORMOSIL were measured. The fluorescent intensity was increased and the trnasmittance was decreased with rising gelling temperature.

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The analysis of impurities in rare earth oxide for fluorescent substance by ICP-MS (ICP-MS에 의한 형광체용 $(Y,\;Eu)_2O_3$ 중 불순물 분석 연구)

  • Kim, Sang-Kyoung;Jang, Seung-Kyu
    • Analytical Science and Technology
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    • v.7 no.1
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    • pp.1-10
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    • 1994
  • A survey was made on detection limit, reproducibility, matrix effect, linear dynamic range and the memory effect of yttrium and europium in order to analyze rare earth elements which exist as impurities in the rare earth oxide which is raw materials of fluorescent substance. When analysing a certain amount of thulium quantitatively using inductively coupled plasma mass spectrometry, it was found that the analysis was interfered with $EuO^+$ which is one of polyatomic ions caused by plasma. As the intensity of thulium linearly proportional to the europium concentration, it was possible to the determine the actual concentration of thulium.

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Enhancement of Fluorescent Properties and Electrochemical Reduction of Coumarin Derivatives (Coumarin 유도체들의 전기화학적 환원과 형광성의 증강)

  • Chun, Hyun Ja;Kim, Sung Hyun;Jung, Eun Joo;Lee, Hye Suk;Kim, Il Kwang
    • Analytical Science and Technology
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    • v.18 no.1
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    • pp.89-95
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    • 2005
  • Studies on the electrochemical reduction of 7-acetoxy-4-bromomethyl-coumarin (ABMC), 7-acetoxymethyl coumarin (AMC), and coumarin in 0.1 M tetraethyl ammonium perchlorate acetonitrile solution were carried out with direct current, differential pulse polarography, cyclic voltammetry, and controlled potential coulometry. The electrochemical reduction of ABMC was proceeded through three irreversible steps coupled with the chemical reactions. The solution color was changed to yellow when the carbonyl group was reduced during second step and the color change was independent with bromo group of ABMC. Fluorescent intensity was highest when the electrochemical reduction was controlled at near the overpotential of supporting electrolyte (-2.3 volts).

A Carbazole Based Bimodal "Turn-On" Fluorescent Probe for Biothiols (Cysteine/Homocysteine) and Fluoride: Sensing, Imaging and its Applications

  • Kaur, Matinder;Yoon, Byungkwon;Kumar, Rajesh;Cho, Min Ju;Kim, Hak Joong;Kim, Jong Seung;Choi, Dong Hoon
    • Bulletin of the Korean Chemical Society
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    • v.35 no.12
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    • pp.3437-3442
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    • 2014
  • A well-known carbazole-based precursor (probe 1) was used for the detection of cysteine/homocysteine and fluoride. Probe 1 shows a "turn-on" response to cysteine/homocysteine and fluoride via enhancement in emission intensity at 442 nm and 462 nm respectively, in solutions and living cells. Furthermore, probe 1 behaves as a fluorescent molecular switch between cysteine/homocysteine and fluoride as the chemical inputs, which have been used for the development of a combinatorial logic circuit and a molecular keypad lock.

Determination of Mercury Ion in Contaminated Soil by Rhodamine B Hydrazide (형광화학센서를 이용한 용출기반 토양 수은 오염조사)

  • Kim, Kyungtae;Park, Junboum
    • Journal of Soil and Groundwater Environment
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    • v.21 no.3
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    • pp.1-5
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    • 2016
  • Rhodamine B Hydrazide as a novel fluorescent and colorimetric probe exhibiting remarkably selective fluorescence enhancement toward Hg2+ ion over other 16 metal ions is herein introduced. The probe reacts with Hg2+ ion followed by its spirolactam ring-opening to give a remarkable enhancement of absorption maximum at 550 nm as well as an enhanced fluorescence intensity at 580 nm in aqueous media. Upon titration with Hg2+ ion in various concentration of 10~200 uM, we found that the probe shows a marked color change from colorless to pink, enabling naked-eye detection toward mercury ion. In addition, in the presence of Hg2+ ion, the probe gave rise to change from non-florescence to strong orange fluorescence (Off-On) with a good linearity of R2=0.97. This preliminary results demonstrate that the fluorescent chemosensor we herein introduced can open a new strategy for marked selective and sensitive detection of mercury ions in contaminated soil containing various metal ions.

The Effect of Color Reproduction Properties at TFT-LCD using High Color Reproduction CCFL

  • Han, Jeong-Min;Ok, Chul-Ho;Seo, Dae-Shik
    • Transactions on Electrical and Electronic Materials
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    • v.8 no.5
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    • pp.215-217
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    • 2007
  • Recently, color reproduction properties have attracted lots of attention with mass production of LCD especially corresponding to TV application and achievement of color reproduction properties such as CRT have been considered one of technical goals for high quality display. However, revision of the color reproduction properties only with CF(color filter) have fundamental limitations and resultant decrease in the transmittance of panel causes demand on high brightness of BL(Back Light). In this paper, we present such a method in which by optimization of original light spectrum from the BL source will improves the color reproduction properties corresponding to them of the CRT. When the intensity of RED and Green-Blue from ramp is revised densely, the characteristics different from CCFL(Cold Cathode Fluorescent Lamp) used before become added so that about 11 % of the color reproduction properties is improved compare to the existing LCD panel.