• 화훼연구
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• 제16권1호
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• pp.44-48
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• 2008

호접란 촉성재배를 위한 하계 고온기 폐광 자연냉풍처리가 개화에 미치는 영향을 밝히고자 본 실험을 실시하여 다음과 같은 결과를 얻었다. 보령지방 폐광의 자연냉풍온도는 $12{\sim}14^{\circ}C$이였으며 이 온도를 주온 $22^{\circ}C$, 야온 $20^{\circ}C$로 조절하여 6월부터 8월까지 처리하였다. 이 온도는 호접란 화성유도 기본 온도범위이었으며, 이 때 외부 평균기온은 $28.4{\sim}32.8^{\circ}C$로 나타났다. 화경 발생은 20일저온경과에서 3% 발생되었으며, 45일 경과에서 65% 이상 발생되었고, 90일 경과에서 100% 발생하였다. 화경장은 30일 경과에서 48.7cm로 짧았으며, 60일 경과에서 62.4cm로 길어져 처리기간이 길수록 화경장이 신장되었다. 화수장, 소화수도 같은 경향이었고, 화경 마디수는 6~7개로 저온 경과일수와는 큰 영향이 없는 것으로 나타났다. 개화는 45일 경과에서 9월 15일에 가장 빨리 개화되었고 저온 경과일수가 길수록 늦어지는 경향을 나타내었다. 고온기 폐광 자연냉풍처리는 무처리에 비하여 화경 발생시기는 3개월, 개화기는 4개월 정도 촉진되었다.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2010년도 심포지엄 및 추계학술발표회
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• pp.455-456
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• 2010

• 한국약용작물학회지
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• 제10권4호
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• pp.263-268
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• 2002

Acifluorfen-tolerant callus lines of Solanum ptycanthum were isolated by stepwise selection. Growth of the unselected line was completely inhibited at 0.5 uM. while some selected lines grew at 8 uM acifluorfen. Twenty-two of twenty-five acifluorfen-tolerant callus lines regenerated shoots. Many of the regenerated somaclones were variants, differing in leaf shape, leaf color, number of flower parts, flower color, and fertility. The acifluorfen tolerant S. ptycanthum callus lines differed.

• 한국작물학회지
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• 제51권spc1호
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• pp.237-241
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• 2006

국내육성 유채품종 간 반수체식물 생산성을 비교하였다. 화아로부터 분리된 소포자를 13%의 sucrose, $0.05mg/{\ell}$ BA 와 $0.5mg/{\ell}$ 의 NAA가 첨가된 NLN 배지에서 배양하였다. 반수체 배의 생산에는 유전형이 중요한 요인이였으며, 탐라유채가 가장 높은 반수체 배 생산능을 보여 화아당 176개의 반수체 배가 발생하였으나, 한라유채와 영산유채는 배 생산은 물론 세포분열도 관찰되지 않았다. 발생한 반수체 배를 NLN배지에서 현탁배양하여 Multilobe abnormal embryos를 형성시켰으며, 계속하여 생장조절제가 첨가되지 않은 MS고체 배지에 치상 배양하여 반수체식물체를 유도 하였다. 재생된 반수체 식물체는 성공적으로 순화되었다.

• 한국작물학회지
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• 제53권2호
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• pp.150-155
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• 2008

양성자와 감마선 조사한 추파형 유채 $M_2$세대의 화뢰의 소포자 배발생에 대해 조사하였다. 세 가지 유채품종 '한라', '내한'과 '탐미' 유채종자를 각각 400 Gy와 600 Gy의 양성자와 감마선으로 전처리 하였다. 일부 종의 양성자와 감마선 조사구에서 배발생이 증가되었고 대조구보다 높았다. 품종별로는 '내한'유채가 배발생 빈도가 가장 높았고 '탐미' 유채가 가장 낮았다. 드물게 배상체로부터 바로 소식물체가 형성되었고 대부분은 비정상적으로 multilobe가 형성되었다. 배상체로부터 식물체가 성공적으로 재분화 되었고 화분에 순화되었다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.191-191
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• 2022

Heterostyly continues to fascinate evolutionary biologists interested in heredity, evolution, breeding, and adaptive function. Polymorphism demonstrates how simply inherited developmental changes in the location of plant sexual associations can have important consequences for population pollination and mating biology. In contrast to homozygous self incompatibility, only a small number of mating phenotypes can be maintained in the population because insect pollinators have limitations in achieving multiple segregation sites for pollen deposition. Field studies of pollen tube growth have shown that reciprocal style-stamen polymorphisms function to increase the capacity of insect-mediated cross-pollination. The genetic pattern of style morphs is well established in various taxa, but despite recent advances, the identity, number, and structure of the genes controlling the heteromorphic syndrome have been poorly elucidated. The phenomenon of heterostyly in buckwheat has been controlled by gene complex concentrate to S-locus. Homomorphic autogamous buckwheat strains were established by the interspecific hybridization. Backcrossing of this line to the common buckwheat (pin) and selecting homostylar progenies made it possible to introduce the self-compatible gene into common buckwheat. In the result, we obtained the BC₉F₂ generation, and defined the strong linkage between flower type and self-incompatibility by microscopic observation of pollen tube growth. This finding suggests that self-incompatibility character is not controlled by one gene. Moreover, we defined the strong linkage between flower type and self-incompatibility. It strongly supports the S supergene theory. Therefore, we have plan to elucidate the heterostyly self-incompatibility by using molecular genetics, proteome analysis and apply to exploitation of buckwheat improvement. In near future, the expression of heterozygous syndromes in genus Fagopyrum with single isolated heterozygous species may provide clues to early stages of polymorphic assembly and shed light on evolutionary models of heterozygous strains.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2021년도 추계학술대회
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• pp.219-219
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• 2021

• 농업과학연구
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• 제42권3호
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• pp.159-165
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• 2015

The cultivation area and use of genetically modified (GM) crops have been increased continuously over the world and concerns about the potential risks of GM crops are also increasing. One of the major concern in risk assessment is the possible development of hybrids through interspecific and intergeneric crosses with related species. This study was conducted to investigate the pollinator have an influence on insect-mediated gene transfer from GM soybeans. Hybrid was induced from GM soybeans by honeybee and western flower thrips, and non-GM soybeans were used as pollen receptor. The analysis for gene-flow was conducted by herbicide selection, immunostrip test, and PCR analysis. In the result of the analysis, three hybrids were detected on the distance 15, 75, 105 cm from pollen source in western flower thrips treatment. In honeybee treatment, one hybrid was detected in the farthest distance (300 cm). These results suggested honeybee and western flower thrips have a possibility they can transfer the introduced gene from GM soybeans to non-GM soybeans.

• 한국약용작물학회지
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• 제17권5호
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• pp.363-368
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• 2009

To fulfill the increasing demand for a high quality of flower, we investigated the effects of nitrogen application on plant growth, yield and bioactive compounds of Chrysanthemum indicum L.. C. indicum L. was cultivated in a pot scale, and nitrogen applied with the level of 0 (N0), 50 (N50), 100 (N100), 150 (N150), 200 (N200) and $300\;(N300)\;kg\;ha^{-1}$ to suggest optimum rate of nitrogen fertilization. Phosphate and potassium applied the same amount of $80-80\;kg\;ha^{-1}$ ($P_2O_5-K_2O$) in all treatments. Growth characteristics and yields of C. indicum L. were significantly affected by nitrogen application. Maximum yield achieved in 265 and $295\;kg\;ha^{-1}$ N treatment on the whole plant and the flower parts, respectively. The nitrogen content and uptake of whole plant significantly increased by the increase of nitrogen application. Five major components of essential oil, $\alpha$-pinene, 1,8-cineol, chrysanthenone, germacrene-D, and $\alpha$-curcumene in flowerheads of C. indicum L. occupied approximately 40% of peak area, germacrene-D decreased by the increase of nitrogen application among them. However, cumambrin A contents in the flower parts of C. indicum L. were affected negatively by the increase of nitrogen application, but total yields of cumambrin A in flower part significantly increased. Conclusively, nitrogen fertilization could increase the yield of flowerheads. The optimum application level of nitrogen fertilizer might be on the range of $265-295\;kg\;ha^{-1}$ in a mountainous soil.

• 한국약용작물학회지
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• 제16권5호
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• pp.287-293
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• 2008

This study was conducted to determine the characteristics of fertilization process and embryo development of Astragalus membranaceus Bunge (Astragali Radix) to provide basic data needed in its breeding. A. membranaceus showed poor seed setting when self-pollination was induced. When artificial pollination was induced, it showed less than 5% bearing in late August, but more than 13% bearing from the beginning of September 4th. The flower size was about $17.0\;mm{\times}4.0\;mm$ and pistils and stamens had the same length of 15.0mm at flowering stage. When self-pollination or cross-pollination was induced, pollen tubes extended to an ovule. While pollen tube was extending to the ovule, reproductive cell split and formed two male generative nuclei and a vegetative nucleus. In the case of self-pollination, fertilized embryo was not observed, but was formed in the case of cross-pollination. A. membranaceus is noted to have zygote self-incompatibility. In the case of cross-pollination, fertilization was observed in 6 to 8 h after pollination, where apical cell derivatives split after fertilization. A spherical pro-embryo was then formed three days after fertilization. The seed attained full shape with a seed coat showing its distinctive contour 15 days after fertilization. Thus, A. membranaceus in Leguminosae family is found to have zygote selfincompatibility although its flower shape is shown to match the self-compatibility plant.