• Molecules and Cells
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• v.40 no.5
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• pp.363-370
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• 2017

Extrahepatic cholangiocarcinoma (ECC), a malignant tumor of biliary origin, has a poor prognosis with limited treatment options. The KRAS oncogene is the most commonly mutated gene in ECC and one of the factors that predicts a poor prognosis and low survival rate. L1 cell adhesion molecule (L1CAM) is expressed in ECC cells and acts as an independent poor prognostic factor in predicting patient survival. In this study we investigate the functional significance of L1CAM in ECC cells with activating KRAS mutation. We selected an ECC cell line, EGI-1, with activating KRAS mutation, and then confirmed its expression of L1CAM by RT-PCR, western blot analysis, and flow cytometry. The suppression of L1CAM expression (using a specific lentivirus-delivered shRNA) significantly decreased the migratory and invasive properties of EGI-1 cells, without altering their proliferation or survival. Analyses of signaling effectors in L1CAM-depleted and control EGI-1 cells indicated that L1CAM suppression decreased the levels of both phosphorylated MKK4 and total MKK4, together with c-Jun N-terminal kinase (JNK) phosphorylation. Further, exposure to a JNK inhibitor (SP600125) decreased migration and invasion of EGI-1 cells. These results suggest that L1CAM promotes cellular migration and invasion via the induction of MKK4 expression, leading to JNK activation. Our study is the first to demonstrate a functional role for L1CAM in ECC carrying the activating KRAS mutation. Given that KRAS is the most commonly mutated oncogene in ECC, L1CAM may serve as an attractive therapeutic target for ECC cells with activating KRAS mutation.

• Proceedings of the Korean Society of Tribologists and Lubrication Engineers Conference
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• 1998.10a
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• pp.190-195
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• 1998

This paper reports on the theoretical and expetimental study of the vane and cam ring motions in a variable displacement vane pump which is already used widely in various industrial and automotive applications. Dynamic equations of vane and cam ring motion and flow continuity equations are derived and then solved simultaneously using the numerical method. Vane detachment cause the pressure tipples, noise, wear in cam ring, and decrease the volumetric efficiency. Consequently, Vane detachment occurs due to excess compression in the pumping chamber, and it can be reduced by adjustment of design parmeters.

• Transactions of the Korean Society of Automotive Engineers
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• v.11 no.3
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• pp.106-114
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• 2003

The pressure ripple in the delivery port is caused by flow ripple, which is induced by variation of pumping chamber volume. The other reason is the reverse flow from the outlet volume produced by pressure difference between pumping chamber and outlet volume, when the pumping chamber is connected with the outlet volume. In this study, a mathematical model is presented for analyzing discharge pressure ripple, which includes vane detachment, cam ring movement , and fluid inertia effects in V-groove in the side plate. From the analysis and experiment, it was found that V-groove on the side plate, coefficient of spring supporting the cam ring, and average discharge pressure are the main factors of discharge pressure ripple in variable displacement vane pump. The theoretical results, provided in this study, were well agreed with experimental results. The analytical model to estimate the magnitude of pressure ripple in this study is expected to be used f3r the optimal design of the variable displacement vane pump.

• Transactions of The Korea Fluid Power Systems Society
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• v.8 no.1
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• pp.10-18
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• 2011

In the beginning of this study, pressure in a cylinder and flow rate from a cylinder of a rotating-cam and fixed-cylinder type radial piston pump are investigated through numerical simulations, so that the simulation results might be utilized as basements for examining physical phenomena occurring in the pump assembly. Then, for supplying basic knowledge on pump design, pressure, flow and leakage characteristics of the pump assembly under the variations of major design parameters are investigated through numerical simulations. At the end, key design parameters influencing upon volumetric efficiency of the pump are listed.

• Journal of computational fluids engineering
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• v.20 no.3
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• pp.35-40
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• 2015

Centrifugal pumps consume considerable amounts of energy in various industrial applications. Therefore, improving the efficiency of pumps machine is a crucial challenge in industrial world. This paper presents numerical investigation of flow characteristics in volutes of centrifugal pumps in order to compare the energy consumption. A wide range of volumetric flow rate has been investigated for each case. The standard k-${\varepsilon}$ is adopted as the turbulence model. The impeller rotation is simulated employing the Multi Reference Frames(MRF) method. First, two different conventional design methods, i.e., the constant angular momentum(CAM) and the constant mean velocity (CMV) are studied and compared to a baseline volute model. The CAM volute profile is a logarithmic spiral. The CMV volute profile shape is an Archimedes spiral curve. The modified volute models show lower head value than baseline volute model, but in case of efficiency graph, CAM curve has higher values than others. Finally for this part, CAM curve is selected to be used in the simulation of different cross-section shape. Two different types of cross-section are generated. One is a simple rectangular shape, and the other one is fan shape. In terms of different cross-section shape, simple rectangular geometry generated higher head and efficiency. Overall, simulation results showed that the volute designed using constant angular momentum(CAM) method has higher characteristic performances than one by CMV volute.

• Journal of Biomedical Engineering Research
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• v.22 no.4
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• pp.349-358
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• 2001

A novel electro-mechanical implantable ventricular assist system is developed as a bridge to transplantation or recovery for patients with end-stage heart failure. The developed system is composed of an implanted blood pump, an external monitoring system which stores data, and a wearable system including a portable external driver and a portable power supply system. The blood pump is designed to be implanted into the left upper abdominal space and provides blood flow from the left ventricular apex to the aorta. The pulsatile blood flow is generated by a double cylindrical cam. There was mo excessive heat emission from the blood pump into the temperature-controlled chamber in the heat test and no stagnated flow within the blood sac by the observation in the flow visualization test. Animal experiments were performed using sheep and calves. The maximum assist flow rate reached 7.85L/min in the animal experiment. The evaluation results showed that the developed system was feasible for the implantable ventricular assist system. The long-term in vitro durability test and mid-term in vivo experiments are in progress and mow the modified next model is under development.

• Journal of Advanced Marine Engineering and Technology
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• v.32 no.4
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• pp.585-595
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• 2008

Process design of multi-stage forging for a bolt with nonaxisymmetric washer cam has been studied by using finite element method. For shape complexity of the bolt, it is impossible to manufacture in a single stage forging. To design multi-stage forging for the bolt the forging load and fiber flow of each step have been analyzed by using commercial finite element code DEFORM-3D. Simulated results have been compared with experimental ones. Multi-stage forging process has been designed with four stages. The workpiece should be eccentric shape until third forging stage. And then bolt head and washer of eccentrical shape is created in last stage. As a results, It was predicted that shape of product would be good and effective strain would be uniformly distributed in the product. Also, it was predicted whether defects would exist or not by reviewing the fiber flow.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.18 no.11
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• pp.2733-2737
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• 2014

Motion detection according to the movement and the change area detection method according to the background difference and the motion history image for use in a motion estimation technique using a real-time image, the motion detection method according to the optical flow, the back-projection of the histogram of the object to track for motion tracking At the heart of MeanShift center point of the object and the object to track, while used, the size, and the like due to the motion tracking algorithm CamShift, Kalman filter to track with direction. In this paper, we implemented the motion detection algorithm based on color and shape information of the object and verify.

• Journal of the Korean Society of Industry Convergence
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• v.23 no.2_2
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• pp.281-288
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• 2020

In this paper, we propose a control method to solve the surface hardness non-uniformity due to flow non-uniformity occurring in the heat treatment process of marine CAM. In the water cooling method including the decarbonization method, an automation device for deformation control has been developed and applied. LSTM was used to estimate the water cooling conditions, and the proposed method was found to be meaningful by improving the prototype results.

• Proceedings of the Korea Information Processing Society Conference
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• 2013.05a
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• pp.319-322
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• 2013

본 연구에서는 이동 객체를 컬러기반에서 추적하는데 있어 주변 환경 변화와 추적중인 객체 색상이 유사한 물체가 존재할 경우 보다 안정적으로 추적할 수 있는 방법을 제시한다. 백그라운드 차영상과 모폴로지 연산을 통하여 이동 객체를 검출하고, 매 프레임마다 발생하는 밝기 및 주변 환경의 영향을 고려하여 기존의 CamShift 알고리즘을 보완하였다. 추적 물체와 색상이 비슷한 주변 물체가 존재할 경우 개선된 CamShift는 불안정한 추적을 보여주었는데 이를 해결하기 위해 Optical Flow기반의 KLT 알고리즘과 병합하는 방법을 제시하였다. 실험 결과를 통해 제안된 추적 방법은 기존의 단점을 보완하였으며 추적성능이 개선됨을 확인하였다.