• Asian Pacific Journal of Cancer Prevention
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• v.15 no.16
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• pp.6627-6632
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• 2014

Purpose: The present study concerns molecular mechanisms involved in induction of apoptosis by a fungal taxol extracted from the fungus Cladosporium oxysporum in T47D human breast cancer cells. Materials and Methods: Apoptosis-induced by the fungal taxol was assessed by MTT assay, nuclear staining, DNA fragmentation, flow cytometry and pro- as well as anti-apoptotic protein expression by Western blotting. Results: Our results showed inhibition of T47D cell proliferation with an $IC_{50}$ value of $2.5{\mu}M/ml$ after 24 h incubation. It was suggested that the extract may exert its anti-proliferative effect on human breast cancer cell line by suppressing growth, arresting through the cell cycle, increase in DNA fragmentation as well as down-regulation of the expression of NF-${\kappa}B$, Bcl-2 and Bcl-XL and up-regulation of pro-apoptotic proteins like Bax, cyt-C and caspase-3. Conclusions: We propose that the fungal taxol contributes to growth inhibition in the human breast cancer cell through apoptosis induction via a mitochondrial mediated pathway, with possible potential as an anticancer therapeutic agent.

• Transactions of the Korean Society of Mechanical Engineers
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• v.12 no.3
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• pp.551-560
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• 1988

Gas-particle two phase flow and heat transfer in a cavity receiving thermal radiation through selectively transparent walls have been analyzed by a finite difference method. Particles injected from the upper hole of the cavity are accelerated downward by gravity and exit through the lower hole while they absorb, emit and scatter the incident thermal radiation. Gas phase is heated through convection heat transfer from particles, and consequently buoyancy induced flow field is formed. Two-equation model with two-way coupling is adopted and interaction terms are treated as sources by PSI-Cell method. For the particulate phase, Lagrangian method is employed to describe velocities and temperatures of particles. As thermal radiation is incident upon horizontally, radiative heat transfer in the vertical direction is assumed negligible and two-flux model is used for the solution of radiative heat flus. Gas phase velocity and temperature distributions, and particle trajectories, velocities and temperatures are presented. The effects of particle inlet condition, particle size, injection velocity and particle mass rate are mainly investigated.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2008.03a
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• pp.659-665
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• 2008

The 1-D numerical study of the interior ballistics has been conducted. The unsteady compressible 1-D CFD code using SIMPLER algorithm and QUICK scheme has been developed. The mathematical model of the two-phase flow has been established for the behavior of the interior ballistics. The moving boundary due to the projectile motion as the physical phenomena of the interior ballistics results in the varied control volume. In order to analyze the moving boundary, the numerical codes, which apply the ghost-cell extrapolation method and the Lagrangian method respectively, have been developed. The ghost-cell extrapolation method has been used in the Eulerian coordinate system. The Lagrangian method has been used in Non-Eulerian coordinate system. These codes have been verified through the analysis of the free piston motion problem in the tube. Through this study, the basic techniques of the numerical code for the multi-dimensional two-phase flow of the interior ballistics have been obtained.

• Archives of Plastic Surgery
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• v.50 no.6
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• pp.568-572
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• 2023

Cutaneous squamous cell carcinoma (CSCC) overlying an arteriovenous fistula (AVF) is rare and presents unique challenges. This case report describes a method of fistula preservation after CSCC excision using a flow-through venous free flap. The saphenous vein of the venous flap was used as flow-through segment for AVF preservation. The flap was inserted along the dorsal aspect of the forearm wound and microvascular anastomosis of the arterial inflow was completed using a vein just proximal to the radiocephalic fistula anastomosis. Venous outflow was established by creating an end-to-end vascular anastomosis between the cephalic vein and the greater saphenous vein. A separate subcutaneous vein was used to provide a low-pressure outflow for the flap to avoid congestion. This case demonstrates an option for AVF preservation that has not been previously described. It also highlights the importance of a multidisciplinary approach for the safe treatment of CSCCs overlying AVFs.

• Transactions of the Korean hydrogen and new energy society
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• v.28 no.6
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• pp.675-682
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• 2017

New government, the market for stationary fuel cell systems in domestic is expected to expand in line with the policy for expanding new and renewable energy. In order to promote and expand the domestic market for stationary fuel cell systems, it is required to do research and develop for cost reduction and efficiency improvement technologies through the localization of BOP. In this study, the safety performance including the power consumption, flow rate, noise and air-tightness of the domestic fuel booster blower and the foreign fuel booster blower was evaluated and the performance improvement of the domestic blower was confirmed. As a result of the power consumption measurement and the flow rate according to the back pressure of the A company 2nd prototype and B company, the values were 73 W, 27 LPM, and 55 W, 25 LPM. These results are attributed to the improvement of performance through design changes such as CAM angle and diaphragm material.

• Journal of the Korean Applied Science and Technology
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• v.27 no.4
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• pp.415-420
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• 2010

This study is focused on the channel design of bipolar plate in the electrode of hydrogen gas generator. The characteristics of hydrogen gas generation was studied in view of efficiency of hydrogen gas generation rate and a tendency of gas flow through the riv design of electrode. Since the flow rate and flow pattern of generated gas in the two phase flow system are the most crucial in determining the efficiency of hydrogen gas generator, we adopted the commercial analytical program of COMSOL MultiphysicsTM to calculate the theoretical flow rate of hydrogen gas from the outlet of gas generator and flow pattern of two phase fluid in the electrode. In this study, liquid electrolyte flows into the bipolar plate and decomposed into gas phase, two phase flow simulation is applied to measure the efficiency of hydrogen gas generation.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.4
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• pp.2325-2331
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• 2013

Objective: To detect effects of plumbagin on proliferation and apoptosis in non-small cell lung cancer cell lines, and investigate the underlying mechanisms. Materials and Methods: Human non-small cell lung cancer cell lines A549, H292 and H460 were treated with various concentrations of plumbagin. Cell proliferation rates was determined using both cell counting kit-8 (CCK-8) and clonogenic assays. Apoptosis was detected by annexin V/propidium iodide double-labeled flow cytometry and TUNEL assay. The levels of reactive oxygen species (ROS) were detected by flow cytometry. Activity of NF-${\kappa}B$ was examined by electrophoretic mobility shift assay (EMSA) and luciferase reporter assay. Western blotting was used to assess the expression of both NF-${\kappa}B$ regulated apoptotic-related gene and activation of p65 and $I{\kappa}B{\kappa}$. Results: Plumbagin dose-dependently inhibited proliferation of the lung cancer cells. The IC50 values of plumbagin in A549, H292, and H460 cells were 10.3 ${\mu}mol/L$, 7.3 ${\mu}mol/L$, and 6.1 ${\mu}mol/L$ for 12 hours, respectively. The compound concentration-dependently induced apoptosis of the three cell lines. Treatment with plumbagin increased the intracellular level of ROS, and inhibited the activation of NK-${\kappa}B$. In addition to inhibition of NF-${\kappa}B$/p65 nuclear translocation, the compound also suppressed the degradation of $I{\kappa}B{\kappa}$. ROS scavenger NAC highly reversed the effect of plumbagin on apoptosis and inactivation of NK-${\kappa}B$ in H460 cell line. Treatment with plumbagin also increased the activity of caspase-9 and caspase-3, downregulated the expression of Bcl-2, upregulated the expression of Bax, Bak, and CytC. Conclusions: Plumbagin inhibits cell growth and induces apoptosis in human lung cancer cells through an NF-${\kappa}B$-regulated mitochondrial-mediated pathway, involving activation of ROS.

• The KSFM Journal of Fluid Machinery
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• v.11 no.6
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• pp.54-63
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• 2008

A study on the flow characteristics in a 4-stage axial compressor and the behavior of rotating stall was experimentally performed at the third-stage rotor and stator rows in order to investigate its performance and instability of the compression system. The pressure losses generated due to the leakage flow at a tip clearance and a shroud seal clearance and the wake flow near the trailing edge of a blade were taken into consideration to estimate the causes of performance drop of the low speed research compressor(LSRC) in Seoul national university. In addition, the measurement of rotating stall was conducted with hot-wire probes and the existence and propagation of stall cell could be confirmed through fast Fourier transform and cross-correlation analysis.

• Journal of the Korean Applied Science and Technology
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• v.27 no.1
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• pp.61-69
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• 2010

This study is focused on the modeling of two phase fluid flow system in the electrode of hydrogen gas generator. The characteristics of hydrogen gas generation was studied in view of efficiency of hydrogen gas generation rate and a tendency of gas flow through the riv of electrode. Since the flow rate of generated gas is the most crucial in determining the efficiency of hydrogen gas generator, we adopted the commercial analytical program of COMSOL $Multiphysics^{TM}$ to calculate the theoretical flow rate of hydrogen gas from the outlet of gas generator.

• Proceedings of the KSME Conference
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• 2003.04a
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• pp.1961-1965
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• 2003

The flow analysis is needed to verify the physical phenomena through interruption processes for improving the capacity and the reliability of gas circuit breakers. Moreover the small current interruption performance of GCBs could be predicted by coupling the flow characteristics with the electric field one. In this paper, the unsteady flow characteristics and the traveling trajectory are depicted with a commercial CFD code, PHOENICS, programmed for moving motion of objects. In order to validate computational results, the measured pressure data in cylinder and in front of arcing contact are compared with the test results of small current interruption.