• Bulletin of the Korean Chemical Society
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• v.27 no.2
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• pp.224-230
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• 2006

Escherichia coli transcription termination factor Rho catalyzes the unwinding of RNA/DNA duplex in reactions that are coupled to ATP binding and hydrolysis. We report here the kinetic mechanism of presteady state ATP binding and hydrolysis by the Rho-RNA complex. Presteady state chemical quenched-flow technique under multiple turnover condition was used to probe the kinetics of ATP binding and hydrolysis by the Rho-RNA complex. The quenched-flow presteady state kinetics of ATP hydrolysis studies show that three ATPs are bound to the Rho-RNA complex with a rate of $4.4\;{\times}\;10^5M^{-1}s^{-1}$, which are subsequently hydrolyzed at a rate of $88s^{-1}$ and released during the initiation process. Global fit of the presteady state ATP hydrolysis kinetic data suggests that a rapid-equilibrium binding of ATP to Rho-RNA complex occurs prior to the first turnover and the chemistry step is not reversible. The initial burst of three ATPs hydrolysis was proposed to be involved in the initialization step that accompanies proper complex formation of Rho-RNA. Based on these results a kinetic model for initiation process for Rho-RNA complex was proposed relating the mechanism of ATP binding and hydrolysis by Rho to the structural transitions of Rho-RNA complex to reach the steady state phase, which is implicated during translocation along the RNA.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.7 no.3
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• pp.471-475
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• 2006

Filling the microchannels is very important in designing micro-injection molding, microdevices, etc. In this paper, flow dynamics was studied in injection molding with microchannels. A transparent PMMA mold was designed and the flow dynamics was observed. The experiment was performed using poly (ethylene oxide) (PEO) and polyacrylamide (PA) aqueous solutions. The transignt dynamic flow and flow competition between the base plate and the microchannels were observed. The flow observation was used to explain previous filling length results in microchannels during micro-injection molding.

• Proceedings of the SAREK Conference
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• 2009.06a
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• pp.1191-1196
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• 2009

The T-s chart of air displays graphically the thermophysical properties, so it is very conveniently used in various thermal systems. In previous study, the software analyzing 31 kinds of values in water system and 32 kinds of values in air-conditioning system were developed. In this study, the software drawing 13 kinds of quantity of state on air properties as ideal gas and analyzing 25 kinds of values in any air system was developed. The 13 kinds of quantity of state on air properties are temperature, pressure, specific volume, specific internal energy, specific enthalpy, specific entropy, specific exergy, exergy ratio, density, isobaric specific heat, isochoric specific heat, ratio of specific heat, and velocity of sound, and the 25 kinds of values including 13 kinds are mass flow rate, volume flow rate, internal energy flow rate, enthalpy flow rate, entropy flow rate, exergy flow rate, heat flow rate, power output, power efficiency, reversible work, lost work, and relative humidity. The developed software can draw any range of chart and analysis any state or process on air system. Also, this supports various document-editing functions such as power point. We wish to this chart is a help to design, analysis, and education in air system field.

• Proceedings of the Korea Water Resources Association Conference
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• 1987.07a
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• pp.113-122
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• 1987

A numerical simulation technique of three-dimensional finite difference model is developed to study the groundwater flow system in Dcjima, an upland area which faces Kasumigaura Lake. For general perspectives of the groundwater flow system, a steady state three-dimentional model is simulated. For the sedimentary mud formations which are found in the representative formation, three situations of hydraulic conductivity are considered, representing an isotropic condition and situations where the horizontal permeability is equal to 10 times and 100times of the vertical one. The finite difference grid used in the simulation has 60x50x30=90,000 nodes. A converged solution with a tolerance of 0.001 meter of hydraulic head is set. Having determined the flow net by using a steady state three-dimensional model. the results for the three cases of hydraulic conductivity are compared with the results of tracer methods (Bae and Kayane 1987) With the aid of four representative vertical cross-sections, groundwater flow systems in the study area are assumed. Water balances for the three cases indicate very good agreement between total recharge and discharge in each case Analyses of groundwater flow system based on the tritium concentrations and water quality measurements (Bae and Kayane 1987) are confirmed by the numerical simulation and the results obtained by these two methods appeared to be in close agreement.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.19 no.3
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• pp.260-266
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• 2006

In this paper, We coupled fluid balance and director balance equation from Ericksen-Leslie's continuum theory and observed the motion of Liquid Crystal molecular. We simulated flow velocity and director distribution in which flow effect is considered in switching on and switching off state. We interpreted the dynamic response characteristic caused by the flow. As the result of the simulation, We could see the flow effect. In the case of Twisted Nematic(TN) cell, this flow caused abnormal twist temporarily in switching off state. We could prove that this abnormal twist is a direct cause of optical bounce phenomenon known well until now with the result of simulation. In addition, We analyzed the mechanism of the fast response due to flow in the case of Optically Compensated Bend(OCB) cell.

• Transactions of the Korean Society of Automotive Engineers
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• v.7 no.7
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• pp.24-31
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• 1999

The MPI engine becomes increasingly popular because it meets two requirements of stringent pollutant emission and the lower fuel consumption. Even though supplies the same amount of fuel to each cylinder , it is hard to precisely control the air-duel ration due to the different amount of air flowing into each cylinder. The uniformity of air-fuel ration in each cylinder is considerably affected by the plenum chamber configuration . This study is focused on experimentally analyzing the flow characteristics within the plenum chamber In the present experiment , steady and valve dynamic state flow tests are performed and the flow field inside the plenum chamber is visualized and measured by utilizing a laser sheet visualization technique and a PTV method. These measured results indicate that the flow structure in the plenum chamber is highly influenced by the plenum chamber configurations, suction flow rates, crank speeds and so on.

• The Journal of the Korea Contents Association
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• v.8 no.8
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• pp.57-64
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• 2008

In this paper, to resolve the problem caused by a network state information inaccuracy the slow delay time that conclusion of network state of one way delay time which accuracy delay time information, according to network state changes on the based TFRC flow control, and suggest that flow control mechanism to adjust transfer rate fit of real time multimedia data. In simulation, to measure of netowork state information that on the average about 12% difference of compared RTT and $OWD{\times}2$. When used RTT, used fair bandwidth TFRC much better than TCP about 32%, when used OWD, difference about 3% used fair bandwidth. Thus, conclusion of accuracy network state that used fair bandwidth according to network state changes on the based TFRC, users can support service of high quality that flow control mechanism to adjust transfer rate fit of real time data.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.46 no.5
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• pp.359-367
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• 2018

The vortex ring state that occurs during the descending flight of a rotorcraft generates a circulating flow like a donut near the rotating surface, and it often causes a rotorcraft fall due to loss of thrust. In this paper, we have physically identified the flow field in the vortex ring state of the quadcopter, one of the types of unmanned aerial vehicles. The descending flight of the quadcopter was simulated in a 1m subsonic wind tunnel of the Korea Aerospace Research Institute(KARI) and the Particle Image Velocimetry(PIV) was used for the flow field measurement. The induced velocity in the hovering state is estimated by using the momentum theory and the test was carried out in the range of descent rate at which the vortex ring condition could be caused. The development and the direction of the vortex ring were confirmed by the measurement of the flow field according to not only the descent rate but also propeller separation distance. In addition, the results of the study show the vortex ring state can be predicted sufficiently by measuring the flow velocity around the quadcopter.

• Coupled systems mechanics
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• v.8 no.2
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• pp.147-168
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• 2019

This work aims to simulate three-dimensional heavy oil flow in a reservoir with heater-wells. Mass, momentum and energy balances, as well as correlations for rock and fluid properties, are used to obtain non-linear partial differential equations for the fluid pressure and temperature, and for the rock temperature. Heat transfer is simulated using a two-equation model that is more appropriate when fluid and rock have very different thermal properties, and we also perform comparisons between one- and two-equation models. The governing equations are discretized using the Finite Volume Method. For the numerical solution, we apply a linearization and an operator splitting. As a consequence, three algebraic subsystems of linearized equations are solved using the Conjugate Gradient Method. The results obtained show the suitability of the numerical method and the technical feasibility of heating the reservoir with static equipment.

• Proceedings of the KIEE Conference
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• 2002.07b
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• pp.622-624
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• 2002

This paper describes a method for steady state hot gas flow analysis considering nozzle ablation for the nozzle of $SF_6$ gas circuit breaker. In order to take account of the effect of ablated nozzle material on the hot gas flow. the PTFE mass concentration equation is added to the established equations for hot gas flow analysis. The steady state simulations were carried out under the condition of DC current flows and the results are presented.