• ETRI Journal
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• 제27권5호
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• pp.539-544
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• 2005

An analog CMOS vision chip for edge detection with power consumption below 20mW was designed by adopting electronic switches. An electronic switch separates the edge detection circuit into two parts; one is a logarithmic compression photocircuit, the other is a signal processing circuit for edge detection. The electronic switch controls the connection between the two circuits. When the electronic switch is OFF, it can intercept the current flow through the signal processing circuit and restrict the magnitude of the current flow below several hundred nA. The estimated power consumption of the chip, with $128{\times}128$ pixels, was below 20mW. The vision chip was designed using $0.25{\mu}m$ 1-poly 5-metal standard full custom CMOS process technology.

• 설비공학논문집
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• 제27권11호
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• pp.587-595
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• 2015

In this study, fault symptoms were simulated and analyzed for a single-effect absorption chiller. The fault patterns of fault detection parameters were tabulated using the fault symptom simulation results. Fault detection and diagnosis by a process history-based method were performed for the in-situ experiment of a single-effect absorption chiller. Simulated fault modes for the in-situ experimental study are the decreases in cooling water and chilled water mass flow rates. Five no-fault reference models for fault detection of a single-effect absorption chiller were developed using fault-free steady-state data. A sensitivity analysis of fault detection using the normalized distance method was carried out with respect to fault progress. When mass flow rates of the cooling and chilled water decrease by more than 19.3% and 17.8%, respectively, the fault can be detected using the normalized distance method, and COP reductions are 6.8% and 4.7%, respectively, compared with normal operation performance. The pattern recognition method for fault diagnosis of a single-effect absorption chiller was found to indicate each failure mode accurately.

• 한국정보통신학회논문지
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• 제18권11호
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• pp.2733-2737
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• 2014

실시간 영상을 이용하여 움직임 검출을 하는데 사용하는 배경 차영상 기법에 의한 움직임 및 변화 영역 검출 방법과 움직임 히스토리에 의한 움직임 검출법, 광류에 의한 움직임 검출법, 움직임 추적을 위한 추적하려는 물체의 히스토그램의 역투영을 이용하면서 물체의 중심점을 추적하는 MeanShift와 물체의 중심, 크기, 방향을 함께 추적하는 CamShift, Kalman 필터에 의한 움직임 추적 알고리즘 등이 있다. 본 논문에서는 물체의 색상과 모양 정보를 이용한 움직임 검출 알고리즘을 구현하고 검증하였다.

• The Plant Pathology Journal
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• 제40권1호
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• pp.40-47
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• 2024

Garlic can be infected by a variety of viruses, but mixed infections with leek yellow stripe virus, onion yellow dwarf virus, and allexiviruses are the most damaging, so an easy, inexpensive on-site method to simultaneously detect at least these three viruses with a certain degree of accuracy is needed to produce virus-free plants. The most common laboratory method for diagnosis is multiplex reverse transcription polymerase chain reaction (RT-PCR). However, allexiviruses are highly diverse even within the same species, making it difficult to design universal PCR primers for all garlic-growing regions in the world. To solve this problem, we developed an inexpensive on-site detection system for the three garlic viruses that uses a commercial mobile PCR device and a compact electrophoresis system with a blue light. In this system, virus-specific bands generated by electrophoresis can be identified by eye in real time because the PCR products are labeled with a fluorescent dye, FITC. Because the electrophoresis step might eventually be replaced with a lateral flow assay (LFA), we also demonstrated that a uniplex LFA can be used for virus detection; however, multiplexing and a significant cost reduction are needed before it can be used for on-site detection.

• 한국인터넷방송통신학회논문지
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• 제14권1호
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• pp.203-209
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• 2014

오늘날 DDoS 공격은 인터넷 안정성에 매우 중요한 위협을 가하고 있다. DDoS 공격은 대량의 트래픽을 네트워크에 전송함으로써 자원을 고갈시키고 정상적인 서비스 제공을 불가능하게 하며 사전 탐지가 힘들고 효율적인 방어가 매우 어렵다. 인터넷과 같은 대규모 망을 대상으로 한 네트워크 공격은 효과적인 탐지 방법이 요구된다. 그러므로 대규모 망에서 침입 탐지 시스템은 효율적인 실시간 탐지가 필요하다. 본 논문에서는 DDoS 공격에 따른 비정상적인 트래픽 범람을 방지하고 합법적인 트래픽 전송을 보장하기 위하여 플로우 정보 분석을 이용한 DDoS 공격 대응 기법을 제안한다. OPNET을 이용해 구현한 결과 DDoS 공격중에 원활한 서비스를 제공할 수 있는 것을 확인하였다.

• 한국수소및신에너지학회논문집
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• 제23권4호
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• pp.316-322
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• 2012

A fuel cell vehicle has the hydrogen detection sensors for checking the hydrogen leakage because it use hydrogen for its fuel and can't use a odorant to protect the fuel cell stack. To verify the hydrogen safety of leakage we select the high possible leak points of fittings in hydrogen storage system and test the leaking behavior at them. The hydrogen leakage flow rate is 10, 40, 118 NL/min and the criterion for maximum hydrogen leakage is based on allowing an equivalent release of combustion energy as permitted by gasoline vehicles in FMVSS301. There are total 18EA hydrogen leakage detection sensors installed in test system. we acquire the hydrogen leakage detection time and determine the ranking. Hydrogen leakage detection time decrease when hydrogen leakage flow rate increase. The minimum hydrogen leakage detection time is about 3 seconds when the flow rate is 118NL/min. In this study, we optimize hydrogen sensor position in fuel cell vehicle and verify the hydrogen leakage safety because there is no inflow inside the vehicle.

• 한국정보통신학회논문지
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• 제23권3호
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• pp.261-266
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• 2019

유동 세포 분석법은 세포나 입자에 대하여 정밀하고 다양한 광학적 특성을 제공해주는 전기적 탐지 기술이다. 형광 처리된 세포나 미립자에 특정한 파장의 빛을 가함으로써 발생 되는 광 산란과 형광 방출을 통해 세포의 크기와 입상도를 포함한 다차원적인 정보를 제공해주는 유동 세포 분석법은 생체 의학 분야 또는 생물 물리학 분야에서 중요한 역할을 수행한다. 그러나 유동 세포 분석법은 고가이며 장비 설치에 있어 적절한 공간이 필요하고 형광 염료 선택에 제한적이라는 단점을 가지고 있다. 따라서 본 논문에서는, 상용화된 유동 세포 분석에 사용되는 고가의 레이저와 운영시스템 대신 발광 다이오드, 마이크로 컨트롤러와 광 검출기를 사용한 저가의 형광세포 측정 시스템을 개발하여 사용자가 원하는 형광 염료에 대한 자유도를 높였다. 또한, 3D 프린터를 사용하여 모듈별 소형화 및 경량화를 통한 사용자 맞춤형 제작이 가능하도록 하였다. 그 결과, 형광처리 한 세포의 양에 변화를 주어 발광도를 측정하였을 때, 높은 선형성이 보임을 확인할 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제31권12호
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• pp.1672-1683
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• 2021

Vibrio parahaemolyticus is recognized as one of the most important foodborne pathogens responsible for gastroenteritis in humans. The bla_CARB-17 gene is an intrinsic β-lactamase gene and a novel species-specific genetic marker of V. parahaemolyticus. In this study, a loop-mediated isothermal amplification (LAMP) assay combined with a lateral flow dipstick (LFD) was developed targeting this bla_CARB-17 gene. The specificity of LAMP-LFD was ascertained by detecting V. parahaemolyticus ATCC 17802 and seven other non-V. parahaemolyticus strains. Finally, the practicability of LAMP-LFD was confirmed by detection with V. parahaemolyticus-contaminated samples and natural food samples. The results showed that the optimized reaction parameters of LAMP are as follows: 2.4 mmol/l Mg²⁺, 0.96 mmol/l dNTPs, 4.8 U Bst DNA polymerase, and an 8:1 ratio of inner primer to outer primer, at 63℃ for 40 min. The optimized reaction time of the LFD assay is 60 min. Cross-reactivity analysis with the seven non-V. parahaemolyticus strains showed that LAMP-LFD was exclusively specific for V. parahaemolyticus. The detection limit of LAMP-LFD for V. parahaemolyticus genomic DNA was 2.1 × 10^-4 ng/μl, corresponding to 630 fg/reaction and displaying a sensitivity that is 100-fold higher than that of conventional PCR. LAMP-LFD in a spiking study revealed a detection limit of approximately 6 CFU/ml, which was similar with conventional PCR. The developed LAMP-LFD specifically identified the 10 V. parahaemolyticus isolates from 30 seafood samples, suggesting that this LAMP-LFD may be a suitable diagnostic method for detecting V. parahaemolyticus in aquatic foods.

• The Plant Pathology Journal
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• 제38권6호
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• pp.665-672
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• 2022

Cymbidium mosaic virus (CymMV) is one of economically important viruses that cause significant losses of orchids in the world. In the present study, a reverse transcription recombinase polymerase amplification (RT-RPA) assay combined with a lateral flow immunostrip (LFI) assay was developed for the detection of CymMV in orchid plants. A pair of primers containing fluorescent probes at each terminus that amplifies highly specifically a part of the coat protein gene of CymMV was determined for RT-RPA assay. The RT-RPA assay involved incubation at an isothermal temperature (39℃) and could be performed rapidly within 30 min. In addition, no cross-reactivity was observed to occur with odontoglossum ringspot virus and cymbidium chlorotic mosaic virus. The RT-RPA with LFI assay (RT-RPA-LFI) for CymMV showed 100 times more sensitivity than conventional reverse transcription polymerase chain reaction (RT-PCR). Furthermore, the RT-PCR-LFI assay demonstrated the simplicity and the rapidity of CymMV detection since the assay did not require any equipment, by comparing results with those of conventional RT-PCR. On-site application of the RT-RPA-LFI assay was validated for the detection of CymMV in field-collected orchids, indicating a simple, rapid, sensitive, and reliable method for detecting CymMV in orchids.

• The Plant Pathology Journal
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• 제39권5호
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• pp.486-493
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• 2023

Cowpea mild mottle virus (CPMMV) is a global plant virus that poses a threat to the production and quality of legume crops. Early and accurate diagnosis is essential for effective managing CPMMV outbreaks. With the advancement in isothermal recombinase polymerase amplification and lateral flow strips technologies, more rapid and sensitive methods have become available for detecting this pathogen. In this study, we have developed a reverse transcription recombinase polymerase amplification combined with lateral flow strips (RT-RPA-LFS) method for the detection of CPMMV, specifically targeting the CPMMV coat protein (CP) gene. The RT-RPA-LFS assay only requires 20 min at 40℃ and demonstrates high specificity. Its detection limit was 10 copies/µl, which is approximately up to 100 times more sensitive than RT-PCR on agarose gel electrophoresis. The developed RT-RPA-LFS method offers a rapid, convenient, and sensitive approach for field detection of CPMMV, which contribute to controlling the spread of the virus.