• BMB Reports
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• v.51 no.4
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• pp.163-164
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• 2018

CONSTANS (CO) induces the expression of FLOWERING LOCUS T (FT) in the photoperiodic pathway, and thereby regulates the seasonal timing of flowering. CO expression is induced and CO protein is stabilized by FLAVIN-BINDING KELCH REPEAT F-BOX PROTEIN 1 (FKF1) in the late afternoon, while CO is degraded by CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) during the night. These regulatory cascades were thought to act independently. In our study, we investigated the relationship between FKF1 and COP1 in the regulation of CO stability in response to ambient light conditions. A genetic analysis revealed that FKF1 acts as a direct upstream negative regulator of COP1, in which cop1 mutation is epistatic to fkf1 mutation in the photoperiodic regulation of flowering. COP1 activity requires the formation of a hetero-tetramer with SUPPRESSOR OF PHYA-105 (SPA1), [$(COP1)_2(SPA1)_2$]. Light-activated FKF1 has an increased binding capacity for COP1, forming a FKF1-COP1 hetero-dimer, and inhibiting COP1 homo-dimerization at its coiled-coil (CC) domain. Mutations in the CC domain result in poor COP1 dimerization and misregulation of photoperiodic floral induction. We propose that FKF1 represses COP1 activity by inhibiting COP1 dimerization in the late afternoon under long-day conditions, resulting in early flowering.

• Journal of Korean Society of Forest Science
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• v.84 no.4
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• pp.495-501
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• 1995

Betula pendula and B. platyphylla var. japonica seedlings and/or grafts growing inside and outside of a plastic-greenhouse were treated with growth regulators to induce flowering at early stages of seedling and graft developments. The seedlings began to develop female catkins visibly in eight to nine months after seeding and in five months after the first treatment of growth regulators. Thirty three percents of the seedlings grown under controlled environment in the plastic-greenhouse with sufficient nutrient supply developed female catkins both in control plants and in the plants treated with IAA, $GA_3$ and kinetin, while none on the control plants grown in the field showed any sign of the development of floral organs. Sixty seven percents of the seedlings treated with ABA and SADH grown in the Plastic-greenhouse developed female catkins. All the seedlings treated with 6.24 mM of SADH developed female catkins. SADH treatment to 2-5 year old seedlings and grafts of birch had a tendency of positive effect on inducing and increasing the flowering of the two birch species.

• Journal of Bio-Environment Control
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• v.28 no.2
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• pp.178-184
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• 2019

Strawberry ($Fragaria{\times}ananassa$) is one of the most important and popular fruit crops in the world, and 'Sulhyang' is one of the principal cultivars cultivated in the Republic of Korea for the domestic market. The growth and flower induction in strawberry is the process which influences directly on fruit bearing and yield of this crop. In this study, effect of benzyladenine (BA), gibberellic acid ($GA_3$), and salicylic acid (SA) on growth and flower bud induction in strawberry 'Sulhyang' was investigated. The 3-week-old runner plants, grown in 21-cell propagation trays, were potted and cultivated in growth chambers with $25^{\circ}C/15^{\circ}C$ (day/night) temperatures, 70% relative humidity (RH), and light intensity of $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ photosynthetic photon flux density (PPFD) provided by white light emitting diodes (LEDs). The runner plants were treated with one of three concentrations, 0 (control), 100, and $200mg{\cdot}L^{-1}$ of BA, $GA_3$, or SA solution. The chemicals were sprayed two times on leaves of runner plants at an interval of two weeks. After 9 weeks the results showed that the application of all chemicals caused reduction of root length and chlorophyll (SPAD) content as compared to the control. The lowest chlorophyll (SPAD) content was recorded in plants treated with $GA_3$. However, the treatment of $200mg{\cdot}L^{-1}$ $GA_3$ promoted leaf area, leaf fresh weight, and plant fresh weight. The greatest flower induction (85%) and number of inflorescences (4.3 inflorescences per plant) were observed in the treatment of $200mg{\cdot}L^{-1}\;SA$, followed by $100mg{\cdot}L^{-1}\;SA$. Overall, results suggest that foliar application of $GA_3$ solution could accelerate plant growth, while foliar application of SA solution could induce hastened flowering. Further studies may be needed to find out the relationship between $GA_3$ and SA solutions treated in a combination, and the molecular mechanism involved in those responses observed.

• Korean Journal of Plant Resources
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• v.18 no.3
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• pp.382-389
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• 2005

This study was conducted to establish the system of effective in vitro propagation by various explant sources culture of Bippeastrum hybridum Hort, 'Dazzler'. We tested the effects of optimal explant source, plant growth regulators on bulblet formation and plant regeneration. Callus was readily produced on the different tissues excised from floral buds whereas, bulbs and shoots were formed only on pedicel explants as compared with anthers, styles and ovaries. Pedicel is the best optimal explant for in vitro propagation. Two distinct pathways, organogenesis through callus and direct bulblet formation, could be recognized in pedicel culture. Up to the $80-100\%$ of bulblet formation and shoot organogenesis from the pedicel in fifteen days before anthesis were effectively induced by MS medium supplemented with 0.5 mg/L NAA and 1.0 mg/L BA. Plantlet regeneration was successfully achieved from pedicel-derived callus, via shoot bud induction or direct bulblet formation. The bulblets with blooming flower were produced within 2 years.

• Molecules and Cells
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• v.25 no.4
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• pp.559-565
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• 2008

Members of the TGA family of basic domain/leucine zipper transcription factors regulate defense genes through physical interaction with NON-EXPRESSOR OF PR1 (NPR1). Of the seven TGA family members, TGA4/octopine synthase (ocs)-element-binding factor 4 (OBF4) is the least understood. Here we present evidence for a novel function of OBF4 as a regulator of flowering. We identified CONSTANS (CO), a positive regulator of floral induction, as an OBF4-interacting protein, in a yeast two-hybrid library screen. OBF4 interacts with the B-box region of CO. The abundance of OBF4 mRNA cycles with a 24 h rhythm under both long-day (LD) and short-day (SD) conditions, with significantly higher levels during the night than during the day. Electrophoretic mobility shift assays revealed that OBF4 binds to the promoter of the FLOWERING LOCUS T (FT) gene, a direct target of CO. We also found that, like CO and FT, an OBF4:GUS construct was prominently expressed in the vascular tissues of leaf, indicating that OBF4 can regulate FT expression through the formation of a protein complex with CO. Taken together, our results suggest that OBF4 may act as a link between defense responses and flowering.

• Korean Journal of Medicinal Crop Science
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• v.2 no.1
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• pp.74-80
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• 1994

This study was carried out to observe the changes in $gibberellin(GA_3)$, indol-3 acetatic acid(IAA) and abscisic acid(ABA) contents known as plant growth regulators in the leaves of Angelica gigas and to get the basic information for the establishment of cultural practice for the inhibition of floral induction. Changes in $GA_3$, IAA and ABA contents were investgated at different growth stage in the young flag leaves of two year old plants.The contents of $GA_3$ showed the highest value by 4. 87mg /kg at early flowering stage, and those were gradually increased from vegetative stage to early flowering stage, but greatly decreased from early flowering stage to fruiting stage. The contents of IAA were also the highest by 2. 27mg /kg at early flowering stage. The contents of ABA were gradually increased along with the development of growth stage. In the fruiting stage those were the highest value by 0.37mg /kg and in early flowering stage the contents were decreased temporarily.

• FLOWER RESEARCH JOURNAL
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• v.16 no.1
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• pp.44-48
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• 2008

This experiment was conducted to examine the effect of injecting cool air from an abandoned mine during the summer time for the growth of Doritaenopsis. The air temperature of abandoned mine in Boryeong was $12{\sim}14^{\circ}C$. The day and night temperatures were set at $22^{\circ}C$ and $20^{\circ}C$, respectively, from June to August in the experimental plastic house. This temperature range was within the suitable range for floral induction in Doritaenopsis. Average outside temperature was $28.4{\sim}32.8^{\circ}C$. The 3% of the crop developed flower stalk in 20 days after the treatment initiation, 65% in 45 days, and 100% in 90 days. The flower stalk length was short (48.7cm) in 30 days and long (62.4cm) in 60 days of treatment. The flower stalk length became longer as time passed. Flower spike and number of florets per stalk displayed the same tendency. Number of nodes was 6~7 and was not affected by the period. The first blooming appeared on 15th of September at 45 days and blooming tended to appear late as the period is lengthened. When the cool air from an abandoned mine was injected, the crop formed flower stalk three months earlier and bloomed four months earlier than the untreated control.