• 한국가축번식학회지
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• 제23권4호
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• pp.313-321
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• 1999

본 연구는 체외생산된 배반포기배의 vitrification 을 위한 용기로서 glass micropipette(GMP)을 이용할 수 있는지, GMP 와 OPS 로 동결융해 후 생존율의 비교 및 GMP vitrification 후 hatching 율의 향상을 위하여 실시하였다. GMP vessel은 열전도율과 수정란을 포함하는 적은 질량 때문에 OPS 보다 동결 및 융해속도를 높일 수 있다. 3개의 체외수정란을 vitrification 용액에 노출시키고 OPS 또는 GMP vessel에 loading 시킨 후 액체질소에 침적하는데까지 20~25초 이내에 실시하였다. 동결ㆍ융해한 배반포기배는 0.25와 15 M sucrose solution 및 TCM 1999에 각각 5분씩 차례로 희석한 후 10% FCS가 첨가된 TCM 199에 24시간동안 배양하였다. OPS(75.9%)와 GMP(80.0%) 방법간의 re-expanding 율은 유의적 (P＜0.05)인 차이가 없었다. OPS(34.1%)와 GMP(37.5%) 방법에서 hatching 율은 intact group(54.3%) 보다도 유의적 (P＜0.05)으로 낮았다. 비록 GMP straw 당 3개 이하의 blastocysts 를 loading 하였더라도 narrow portion(83.3%) 보다도 wide portion(S6.7%)에서 vitrified 되었다면 re-expanding 율이 유의적 (P＜0.05)으로 낮았다. 비록 30초 처리군과 무처리군 간에는 유의적인 차이가 없었지만 0.05% pronase 용액에 30, 60 및 90초간 처리군 (45.9, 54.7 및 57.5%)의 hatching 율은 무처리구 (35.0%) 보다 유의적 (P＜0.05)으로 높았다. 이러한 결과들은 OPS와 GMP vitrification vessel은 체외생산된 배반포기배의 높은 생존율을 얻을 수 있다. 그러나 GMP vessel은 L$N_2$침적 후 vessel의 floating을 방지하기 위한 또 다른 cap 이 필요하지 않다는 유리한 점을 가지고 있다. 수정란의 loading 위치, 즉 narrow 또는 wide portion에 따라 소 체외 생산된 배반포기배의 생존력에 제한적인 요인으로 고려된다. 0.5% pronase 용액에 60 또는 90초간의 노출은 융해후 hatching 율을 향상시킬 수 있었다.

• 동의생리병리학회지
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• 제23권5호
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• pp.1193-1198
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• 2009

Pulse diagnosis is a central diagnosis method used in traditional Oriental medicine. To standardize and modernize the pulse diagnosis method, it is essential to develop an instrument-based reinterpretation of the clinically used pulse images in terms of the physical quantities such as the strength, period, width, length, and depth of the pulse. As a step towards such standardization, we conducted a clinical study on the floating/sinking pulses based on an automated palpation instrument (3D-MAC, Daeyo Medi, Korea) for 213 female subjects in their 20s and 174 female subjects in their 60s. The floating/sinking pulses are the two representative pulse images depending only on the depth of the pulse, and can be conveniently scaled by the coefficient of the floating-sinking pulse ($C_{fs}{\in}(0,1)$), which represents how strong one should apply the hold-down pressure to obtain the maximal pulse strength. As a result, primarily we found that it tends to appear more floating-like pulse ($C_{fs}{\rightarrow}0$) at Gwan and more sinking-like pulse ($C_{fs}{\rightarrow}1$) at Cheek, at both age groups and at both wrists. This result is consistent with a previous study on the geometrical structure of the blood vessel by an ultrasonograph. Second, the pulse tends to be more sinking-like in the age group of 60s than 20s. Finally, the pulses at the right palpation positions were found to be more sinking-like than the left, at both age groups.

• Journal of Advanced Research in Ocean Engineering
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• 제1권4호
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• pp.239-251
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• 2015

In this study, a spectral fatigue analysis was performed for the topside structure of an offshore floating vessel. The topside structure was idealized using beam elements in the SACS program. The fatigue analysis was carried out considering the wave and wind loads separately. For the wave-induced fatigue damage calculation, motion RAOs calculated from a direct wave load analysis and regular waves with different periods and unit wave heights were utilized. Then, the member end force transfer functions were generated covering all the loading conditions. Stress response transfer functions at each joint were produced using the specified SCFs and member end force transfer functions. fatigue damages were calculated using the obtained stress ranges, S-N curve, wave spectrum, heading probability of each loading condition, and their corresponding occurrences in the wave scatter diagrams. For the wind induced fatigue damage calculation, a dynamic wind spectral fatigue analysis was performed. First, a dynamic natural frequency analysis was performed to generate the structural dynamic characteristics, including the eigenvalues (natural frequencies), eigenvectors (mode shapes), and mass matrix. To adequately represent the dynamic characteristic of the structure, the number of modes was appropriately determined in the lateral direction. Second, a wind spectral fatigue analysis was performed using the mode shapes and mass data obtained from the previous results. In this analysis, the Weibull distribution of the wind speed occurrence, occurrence probability in each direction, damping coefficient, S-N curves, and SCF of each joint were defined and used. In particular, the wind fatigue damages were calculated under the assumption that the stress ranges followed a Rayleigh distribution. The total fatigue damages were calculated from the combination with wind and wave fatigue damages according to the DNV rule.

• 한국해양공학회지
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• 제31권4호
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• pp.266-273
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• 2017

In recent years, LNG bunkering terminals are needed to supply LNG as fuel to meet the emission requirements of the International Maritime Organization (IMO). A floating LNG bunkering terminal (FLBT) is one of the most cost-effective and environmentally friendly LNG bunkering systems for storing LNG and transferring it directly to an LNG fuel vessel. The FLBT maintains its position using mooring systems such as spread mooring and turret mooring. The loads on the vessel and mooring lines must be carefully determined to maintain their positions within the operable area. In this study, the wind loads acting in several side-by-side arrangements on the FLBT and LNG-BS were estimated using wind tunnel tests in the Force Technology, and the shielding effect due to the presence of ships upstream was evaluated. In addition, the empirical formulations proposed by Fujiwara et al. (2012) were used to estimate the wind force coefficients acting on the FLBT and those results were compared with experimental results.

• 대한조선학회 특별논문집
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• 대한조선학회 2009년도 특별논문집
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• pp.24-34
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• 2009

The latest trend of development for submarine oil field is caused by the drained oil and gas for ground field, and FPSO is a keyword as the development for submarine oil field. FPSO (Floating Production Storage Offloading) is a kind of vessel type have a topside system for production of oil and gas and store them until the oil or gas can be offloaded onto a tanker or transported through a pipeline. Prior to the introduction of reinforcement under the pull-in winch on the STP extended top as the object of this paper, the technical background shall be introduced such as FPSO and the system and main equipments for STP as follows. The original structural concept for reinforcement of pull-in winch on the STP top and extended structure on moonpool was proposed by buyer's engineering team but it was much modified and improved in accordance with builder's fabrication and construction method.

• 한국해양공학회지
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• 제24권1호
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• pp.20-33
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• 2010

The paper deals with strength design of a riser support installed on floating production storage and offloading (FPSO) vessel under various loading conditions - operation, extreme, damaged, one line failure case (OLFC) and installation. The design problem is formulated such that thickness sizing variables are determined by minimizing the weight of a riser support structure subject to stresses constraints. The initial design model is generated based on an actual FPSO riser support specification. The finite element analysis (FEA) is conducted using MSC/NASTRAN, and optimal solutions are obtained via moving least squares method (MLSM) in the context of response surface based approximate optimization. For the meta-modeling of inequality constraint functions of stresses, a constraint-feasible moving least squares method (CF-MLSM) is used in the present study. The method of CF-MLSM, compared to a conventional MLSM, has been shown to ensure the constraint feasibility in a case where the approximate optimization process is employed. The optimization results present improved design performances under various riser operating conditions.

• 한국해양공학회지
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• 제32권1호
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• pp.51-61
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• 2018

In this study, the operability of an FLBT (floating LNG bunkering terminal) was evaluated experimentally. Model tests were conducted in the KRISO (Korea Research Institute of Ships and Ocean Engineering) ocean engineering basin. An FLBT, an LNG carrier, and two LNG bunkering shuttles were moored side by side with mooring ropes and fenders. Two white-noise wave cases, one irregular wave case, and various regular wave cases were generated. The relative local motions between each LNG loading arm and its corresponding manifold in the initial design configuration were calculated from measured 6-DOF motions at the center of gravity of each of the four vessels. Furthermore, the locations of the LNG loading arms and manifolds were varied to minimize the relative local motions.

• Journal of Advanced Marine Engineering and Technology
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• 제38권2호
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• pp.195-201
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• 2014

Flexible risers have been used extensively in recent years for floating and early production systems. Such risers offer the advantage of having inherent heave compliance in their catenary thereby greatly reducing the complexity of the riser-to-rig and riser-to subsea interfaces. Another advantage with flexible risers is their greater reliability. Concerns about fatigue life, gas permeation and pigging of lines have been overcome by extensive experience with these risers in production applications. In this paper, flexible riser analysis results were compared through coupled and uncoupled dynamic analyses methods. A time domain coupled analysis capability has been developed to model the dynamic responses of an integrated floating system incorporating the interactions between vessel, moorings and risers in a marine environment. For this study, SPM (Single Point Mooring) system for an FSU in shallow water was considered. This optimization model was integrated with a time-domain global motion analysis to assess both stability and design constraints of the flexible riser system.

• 한국연안방재학회지
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• 제1권4호
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• pp.149-155
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• 2014

Flexible risers have been used extensively in recent years for floating and early production systems. Such risers offer the advantage of having inherent heave compliance in their catenary thereby greatly reducing the complexity of the riser-to-rig and riser-to subsea interfaces. Another advantage with flexible risers is their greater reliability. Concerns about fatigue life, gas permeation and pigging of lines have been overcome by extensive experience with these risers in production applications. In this paper, flexible riser analysis results were compared through coupled and uncoupled dynamic analyses methods. A time domain coupled analysis capability has been developed to model the dynamic responses of an integrated floating system incorporating the interactions between vessel, moorings and risers in a marine environment. For this study, SPM (Single Point Mooring) system for an FSU in shallow water was considered. This optimization model was integrated with a time-domain global motion analysis to assess both stability and design constraints of the flexible riser system.

• 해양환경안전학회지
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• 제28권spc호
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• pp.11-17
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• 2022

본 논문에서는 해상 위험유해물질(Hazardous Noxious Substances, HNS) 사고의 효과적인 대응을 위해 개발된 부유식 무인이동체기반 광역탐지 및 모니터링 시스템의 운용 시나리오 설계와 실험 검증 내용을 보인다. 광역탐지 및 모니터링 시스템은 장시간 운용이 가능하되 제한적 이동이 가능한 무계류형 부이 형태를 갖는 부유식 무인이동체 플랫폼을 기반으로 개발되었으며 임무 수행에 필요한 열화상 카메라, 레이더, 부유 및 대기 HNS의 탐지를 위한 센서가 탑재되었다. 실험 검증 과정에서는 탐지 센서 성능을 야외 환경에서 실험적으로 검증하기 위해 이동식 가스 유출 시스템(Portable Gas-exposure System, PGS)을 추가로 설치하였다. 무인 시스템의 원격 및 자율 운용을 위해 전체 운용 소프트웨어는 로봇운영체제(Robot Operating System, ROS) 프레임워크를 기반으로 통합되었다. 내수면 및 실해역에서의 실험을 통해 개발된 시스템의 운용 및 활용 가능성을 실험적으로 검증하였다.