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Radioisotope Labelling Method of Pine Gall Midge (Thecodiplosis japonensis UCHIDA et INOUYE) (솔잎혹파리에 대한 방사선동위원소 표식방법에 관한 연구)

  • Kwon S. H.;Chung K. H.;Ryu J.
    • Korean journal of applied entomology
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    • v.17 no.3 s.36
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    • pp.155-160
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    • 1978
  • This experiment was carried out to develope a technique for labelling pine gall midge with ·radioactive phosphorus and calcium. This technique evolved would help to obtain basic information on the appropriate concentration of $^{32}P\; and\; ^{45}Ca$ in tagging and determination of adult flight ranges which considered as one of the factors in dissemination. 1. Labelling at the larval stage of pine gall midge by injection of $^{45}Ca$ into pine tree trunk appeared not to be effective for tagging the adults because radioactivity was gradually decreased to background by emergence. 2. The radioactivities of larvae in the galls gradually increased in September but from October 1 the activity was gradually decreased for 10 days then equilibrium was maintained thereafter. The results seem to indicate that larval diet termination occured early October. 3. A maximum level of radiation was detected at 3 weeks after $^{32}P$ injection and thereafter it was gradually reduced. This result implies that $^{32}P$ is not appropriate radioisotope in labelling pine gall midge through trunk injection method. 4. After washing in running water for 10 minutes, the optimum$^{32}P$ concentration for treated overwintered larvae and pupae was found to be 0.5 micro curie per ml. for 30 minutes dipping.

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A Research on the Relationship between the Perception of Administrative Regulation and Odor Acceptance among Residents who live in Ochang Industrial Complex Area (오창산업단지 주변지역 주민의 행정규제에 대한 인식과 악취수용성의 상관성 연구)

  • Park, Sung-Sik;Jung, Ju-Yong;Yeon, Ik-Jun
    • Journal of Environmental Impact Assessment
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    • v.24 no.4
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    • pp.332-343
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    • 2015
  • It would be the most effective way to have government take care of social problems and hazardous material danger due to odor. It is also well known that odor acceptance and their willingness to pay for resolving the odor problem vary person by person. This is why public sector's intervention is required to get ride of any possibility of market failure resulted from negative external effect of human. This paper has tried to do empirical research based on survey of residents who are currently living in the area of Ochang industrial complex in North Chong-chung province. According to the empirical research results, first of all, it was proven residents' perception of legal regulation has a negative effect on odor acceptance. That is, the more residents are aware of law and regulation, the less they tend to accept odor. In addition, resident's perception on need to improve odor regulation has a positive impact on their odor acceptance. Finally, residents' satisfaction on the legal complaints to local government has an interaction effect to causative relationship between the perception of improving odor regulation and odor acceptance.

Identification and Safety Assessment of Cucumber Mosaic Virus Coat Protein in Genetically Modified Pepper (Capsicum annuum)

  • Kim, Eunji;Noh, Hee Min;Phat, Chanvorleak;Lee, Gung Pyo;Kim, Jun Hong;Park, Tae-Sung;Lee, Chan
    • Horticultural Science & Technology
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    • v.34 no.6
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    • pp.924-939
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    • 2016
  • The great economic losses caused by Cucumber mosaic virus (CMV) infection of peppers has led to the development of genetically modified (GM) CMV-resistant peppers. We developed virus-resistant pepper plants using Agrobacterium tumefaciens -mediated transformation. The expressed recombinant protein was purified using nickel-nitrilotriacetic acid resin and immunoaffinity chromatography, and purity was assessed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Immunoblot analysis revealed the purified CMV coat protein (CMV-CP) had a molecular mass of 25 kDa. After in-gel digestion and desalting, the internal peptide fragments of CMV-CP were sequenced by matrix-assisted laser desorption/ionization-time of flight. Most GM pepper and Escherichia coli BL21 internal peptides had identical peptide sequences and contained 137 of 183 whole peptides in CMV-CP. A quantitative enzyme-linked immunosorbent assay was performed to detect CMV-resistant GM peppers. We also provide basic information about the expressed protein in GM peppers for further safety assessment. The contents of soluble protein and CMV-CP were measured in GM and control peppers cultivated in three different areas of Korea. Statistical significance in terms of cultivation areas, harvest times, generations, and plant tissue origin were determined based on a P value of 0.05. The highest amount of CMV-CP was detected at the seedling stage from plant grown in each region. T3 and T5 showed significantly different levels of CMV-CP from T4 in leaves in the whorl stage. No statistical differences were observed among GM peppers at different stages of maturity in any cultivation area. The results from this study contribute to the safety evaluation of newly designed CMV-resistant GM peppers and provide a standard against which to compare other virus-resistant GM peppers.

Chemical and bioactive comparison of flowers of Panax ginseng Meyer, Panax quinquefolius L., and Panax notoginseng Burk.

  • Li, Fang;Lv, Chongning;Li, Qiao;Wang, Jing;Song, Dan;Liu, Pengpeng;Zhang, Dandan;Lu, Jincai
    • Journal of Ginseng Research
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    • v.41 no.4
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    • pp.487-495
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    • 2017
  • Background: Although flowers of Panax ginseng Meyer (FPG), Panax quinquefolius L. (FPQ), and Panax notoginseng Burk. (FPN) have been historically used as both medicine and food, each is used differently in practice. Methods: To investigate the connection between components and enhancing immunity activity of FPG, FPQ, and FPN, a method based on a rapid LC coupled with quadrupole time-of-flight MS and immunomodulatory activity study evaluated by a carbon clearance test were combined. Results: According to quantitative results, the ratio of the total content of protopanaxatiol-type ginsenosides to protopanaxadiol-type ginsenosides in FPN was 0, but ranged from 1.10 to 1.32 and from 0.23 to 0.35 in FPG and FPQ, respectively. The ratio of the total content of neutral ginsenosides to the corresponding malonyl-ginsenosides in FPN ($5.52{\pm}1.33%$) was higher than FPG ($3.2{\pm}0.64%$) and FPQ ($2.39{\pm}0.57%$). The colorimetric analysis showed the content of total ginsenosides in FPQ, FPG, and FPN to be $13.75{\pm}0.60%$, $17.45{\pm}0.42%$, and $12.45{\pm}1.77%$, respectively. The carbon clearance assay indicated that the phagocytic activity of FPG and FPQ was higher than that of FPN. A clear discrimination among FPG, FPQ, and FPN was observed in the principal component analysis score plots. Seven compounds were confirmed to contribute strongly by loading plots, which may be the cause of differences in efficacy. Conclusion: This study provides basic information about the chemical and bioactive comparison of FPG, FPQ, and FPN, indicating that protopanaxtriol-type ginsenosides and malonyl-ginsenosides may play a key role in their enhancing immunity properties.

Converged Influencing Factors on the Stages of Exercise Behavior Change of Nurses in Shift Work Using Transtheoretical Model (범이론적모델을 이용한 교대근무 간호사의 운동행위 변화단계에 미치는 융합적인 영향요인)

  • Lee, Hyea-Kyung;Chung, Young-Jin
    • Journal of Convergence for Information Technology
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    • v.7 no.6
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    • pp.35-43
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    • 2017
  • The purpose of this study was to analyze the converged influencing factors on the stages of exercise behavior changes in shift work nurses. The subjects were 120 shift work nurses working in hospitals who understood the purpose of the study and agreed to participate in the study. Data were analyzed using frequency, percentage, mean, standard deviation, t-test, $X^2$ test, and multiple logistic regression analysis. The exercise motivation factors of shift work nurses had a significant effect on stages of exercise behavior change (${\beta}=2.480$, p=.022), health status perceptions(${\beta}=1.151$, p=.013). Social support factors (${\beta}=1.819$, p=.002) and marital status (${\beta}=-1.820$, p=.004) also had significant effects on the stages of exercise behavior change of shift work nurses. In other words, subjects with change in exercise behavior had 11.9 times higher motivation and 3.1 times higher health status perception than those without change in exercise behavior, social support was 6.1 times higher, and unmarried subjects showed 16 times showed higher than that of married subjects. Therefore, it is important to develop a strategy to practice continuous and regular exercise in consideration of the exercise motivation, social support, and health status perception of the nurses in shift work.

Use of Unmanned Aerial Vehicle for Forecasting Pine Wood Nematode in Boundary Area: A Case Study of Sejong Metropolitan Autonomous City (무인항공기를 이용한 소나무재선충병 선단지 예찰 기법: 세종특별자치시를 중심으로)

  • Kim, Myeong-Jun;Bang, Hong-Seok;Lee, Joon-Woo
    • Journal of Korean Society of Forest Science
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    • v.106 no.1
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    • pp.100-109
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    • 2017
  • This study was conducted for preliminary survey and management support for Pine Wood Nematode (PWN) suppression. We took areal photographs of 6 areas for a total of 2,284 ha during 2 weeks period from 15/02/2016, and produced 6 ortho-images with a high resolution of 12 cm GSD (Ground Sample Distance). Initially we classified 423 trees suspected for PWN infection based on the ortho-images. However, low accuracy was observed due to the problems of seasonal characteristics of aerial photographing and variation of forest stands. Therefore, we narrowed down 231 trees out of the 423 trees based on the initial classification, snap photos, and flight information; produced thematic maps; conducted field survey using GNSS; and detected 23 trees for PWN infection that was confirmed by ground sampling and laboratory analysis. The infected trees consisted of 14 broad-leaf trees, 5 pine trees (2 Pinus rigida), and 4 other conifers, showing PWN infection occurred regardless of tree species. It took 6 days for 2.3 men from to start taking areal photos using UAV (Unmanned Aerial Vehicle) to finish detecting PNW (Pine Wood Nematode) infected tress for over 2,200 ha, indicating relatively high efficacy.

Comparative Analysis of Focal Length Bias for Three Different Line Scanners (초점거리 편의가 지상 정확도에 미치는 영향 비교 연구 - 세가지 라인 스캐너를 대상으로 -)

  • Kim, Changjae
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.32 no.4_1
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    • pp.363-371
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    • 2014
  • Most space-borne optical scanning systems adopt linear arrayconfigurations. The well-knownthree different types of space-borne sensors arealong-track line scanner, across-track linescanner, and three line scanner. To acquire accurate location information of an object on the ground withthose sensors, the exterior and interior orientation parameters are critical factors for both of space-borne and airborne missions. Since the imaging geometry of sensors mightchange time to time due to thermal influence, vibration, and wind, it is very important to analyze the Interior Orientation Parameters (IOP) effects on the ground. The experiments based on synthetic datasets arecarried out while the focal length biases are changing. Also, both high and low altitudes of the imagingsensor were applied. In case with the along-track line scanner, the focal length bias caused errors along the scanline direction. In the other case with the across-track one, the focal length bias caused errors alongthe scan line and vertical directions. Lastly, vertical errors were observed in the case ofthree-line scanner. Those results from this study will be able to provide the guideline for developing new linearsensors, so as for improving the accuracy of laboratory or in-flight sensor calibrations.

Development of an Image Processing System for the Large Size High Resolution Satellite Images (대용량 고해상 위성영상처리 시스템 개발)

  • 김경옥;양영규;안충현
    • Korean Journal of Remote Sensing
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    • v.14 no.4
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    • pp.376-391
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    • 1998
  • Images from satellites will have 1 to 3 meter ground resolution and will be very useful for analyzing current status of earth surface. An image processing system named GeoWatch with more intelligent image processing algorithms has been designed and implemented to support the detailed analysis of the land surface using high-resolution satellite imagery. The GeoWatch is a valuable tool for satellite image processing such as digitizing, geometric correction using ground control points, interactive enhancement, various transforms, arithmetic operations, calculating vegetation indices. It can be used for investigating various facts such as the change detection, land cover classification, capacity estimation of the industrial complex, urban information extraction, etc. using more intelligent analysis method with a variety of visual techniques. The strong points of this system are flexible algorithm-save-method for efficient handling of large size images (e.g. full scenes), automatic menu generation and powerful visual programming environment. Most of the existing image processing systems use general graphic user interfaces. In this paper we adopted visual program language for remotely sensed image processing for its powerful programmability and ease of use. This system is an integrated raster/vector analysis system and equipped with many useful functions such as vector overlay, flight simulation, 3D display, and object modeling techniques, etc. In addition to the modules for image and digital signal processing, the system provides many other utilities such as a toolbox and an interactive image editor. This paper also presents several cases of image analysis methods with AI (Artificial Intelligent) technique and design concept for visual programming environment.

Safety Risk Management Policy of United States small unmanned aerial system (미 소형 무인비행체계의 안전성 위험관리 정책)

  • Hong, Jin-Keun
    • Journal of Convergence for Information Technology
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    • v.11 no.9
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    • pp.35-42
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    • 2021
  • The purpose of this paper is to review the small unmanned aerial system (sUAS) safety policy promoted by the United States(US) government. Therefore, in this paper, along with sUAS risk factors, the risk factors of sUAS that the US government is interested in are described. In addition, the risk factors were classified into physical and non-physical factors, and provisions mentioned in the Federal Aviation Administration(FAA) Relicensing Act were reviewed. Other risk scenarios were analyzed focusing on target scenario items that the FAA is interested in, such as flight operation disruption, infrastructure damage, and facility trespassing. Of course, we looked at the risk management principles promoted by the US FAA. In this paper, as a research method, the direction and contents of the FAA's sUAS policy were studied and reviewed from the analysis of major foreign journals and policy. In the research result of this paper, by analyzing the FAA sUAS safety risk management policy, the integrated operation and safety policy, physical risk management policy, operation and safety regulation, and sUAS policy and technology direction necessary for establishing the sUAS safety risk management guide in Korea are presented. The contribution of this study is to identify the leading US sUAS safety policy direction, and it can be used as basic data for deriving future domestic policy directions from this. Based on the research results presented in the future, policy studies are needed to derive detailed implementation plans.

Evaluation of Possibility of Large-scale Digital Map through Precision Sensor Modeling of UAV (무인항공기 정밀 센서모델링을 통한 대축척 수치도화 가능성 평가)

  • Lim, Pyung-chae;Kim, Han-gyeol;Park, Jimin;Rhee, Sooahm
    • Korean Journal of Remote Sensing
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    • v.36 no.6_1
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    • pp.1393-1405
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    • 2020
  • UAV (Unmanned Aerial Vehicle) can acquire high-resolution images due to low-altitude flight, and it can be photographed at any time. Therefore, the UAV images can be updated at any time in map production. Due to these advantages, studies on the possibility of producing large-scale digital maps using UAV images are actively being conducted. Precise digital maps can be used as base data for digital twins or smart cites. For producing a precise digital map, precise sensor modeling using GCPs (Ground Control Points) must be preceded. In this study, geometric models of UAV images were established through a precision sensor modeling algorithm developed in house. Then, a digital map by stereo plotting was produced to evaluate the possibility of large-scale digital map. For this study, images and GCPs were acquired for Ganseok-dong, Incheon and Yeouido, Seoul. As a result of precision sensor modeling accuracy analysis, high accuracy was confirmed within 3 pixels of the average error of the checkpoints and 4 pixels of the RMSE was confirmed for the two study regions. As a result of the mapping accuracy analysis, it satisfied the 1:1,000 mapping accuracy announced by the NGII (National Geographic information Institute). Therefore, the precision sensor modeling technology suggested the possibility of producing a 1:1,000 large-scale digital map by UAV images.