• Journal of Microbiology and Biotechnology
• /
• 제23권6호
• /
• pp.766-770
• /
• 2013

Several upstream activators required for proper activation of brlA are involved in the development, vegetative growth, toxin production, and pathogenesis of Aspergillus fumigatus. In this study, we characterized the roles of two upstream developmental regulators, A. fumigatus flbB (AfuflbB) and flbE (AfuflbE), in toxin production and virulence. The deletion of AfuflbB and AfuflbE resulted in reduction of the expression of AfulaeA. Moreover, only about 8% to 10% of fumagillin was produced in the two mutants compared with that of wild type, and ${\Delta}AfuflbB$ strain produced 85% of gliotoxin compared with wild type, whereas none was produced by ${\Delta}AfuflbB$. Flow-cytometric analysis revealed decreased necrotic and apoptotic polymorphonuclear leukocytes cell death after exposure to supernatants from ${\Delta}AfuflbB$ and ${\Delta}AfuflbB$ strains compared with the wild type. These results indicate that FlbB and FlbE are necessary for the proper laeA expression, toxin production, and virulence of A. fumigatus.

• 한국균학회소식:학술대회논문집
• /
• 한국균학회 2014년도 추계학술대회 및 정기총회
• /
• pp.15-15
• /
• 2014

Fusarium graminearum (teleomorph Gibberella zeae) is an important plant pathogen that causes head blight of major cereal crops such as wheat, barley, and rice, as well as causing ear and stalk rot on maize worldwide. Plant diseases caused by this fungus lead to severe yield losses and accumulation of harmful mycotoxins in infected cereals [1]. Fungi utilize spore production as a mean to rapidly avoid unfavorable environmental conditions and to amplify their population. Spores are produced sexually and asexually and their production is precisely controlled. Upstream developmental activators consist of fluffy genes have been known to orchestrate early induction of condiogenesis in a model filamentous fungus Aspergillus nidulans. To understand the molecular mechanisms underlying conidiogenesis in F. graminearum, we characterized functions of the F. graminearum fluffy gene homologs [2]. We found that FlbD is conserved regulatory function for conidiogenesis in both A. nidulans and F. graminearum among five fluffy gene homologs. flbD deletion abolished conidia and perithecia production, suggesting that FlbD have global roles in hyphal differentiation processes in F. graminearum. We further identified and functionally characterized the ortholog of AbaA, which is involved in differentiation from vegetative hyphae to conidia and known to be absent in F. graminearum [3]. Deletion of abaA did not affect vegetative growth, sexual development, or virulence, but conidium production was completely abolished and thin hyphae grew from abnormally shaped phialides in abaA deletion mutants. Overexpression of abaA resulted in pleiotropic defects such as impaired sexual and asexual development, retarded conidium germination, and reduced trichothecene production. AbaA localized to the nuclei of phialides and terminal cells of mature conidia. Successful interspecies complementation using A. nidulans AbaA and the conserved AbaA-WetA pathway demonstrated that the molecular mechanisms responsible for AbaA activity are conserved in F. graminearum as they are in A. nidulans. F. graminearum ortholog of Aspergillus nidulans wetA has been shown to be involved in conidiogenesis and conidium maturation [4]. Deletion of F. graminearum wetA did not alter mycelial growth, sexual development, or virulence, but the wetA deletion mutants produced longer conidia with fewer septa, and the conidia were sensitive to acute stresses, such as oxidative stress and heat stress. Furthermore, the survival rate of aged conidia from the F. graminearum wetA deletion mutants was reduced. The wetA deletion resulted in vigorous generation of single-celled conidia through autophagy-dependent microcycle conidiation, indicating that WetA functions to maintain conidia dormancy by suppressing microcycle conidiation in F. graminearum. In A. nidulans, FlbB physically interacts with FlbD and FlbE, and the resulting FlbB/FlbE and FlbB/FlbD complexes induce the expression of flbD and brlA, respectively. BrlA is an activator of the AbaA-WetA pathway. AbaA and WetA are required for phialide formation and conidia maturation, respectively [5]. In F. graminearum, the AbaA-WetA pathway is similar to that of A. nidulans, except a brlA ortholog does not exist. Amongst the fluffy genes, only fgflbD has a conserved role for regulation of the AbaA-WetA pathway.

• 한국산학기술학회논문지
• /
• 제13권9호
• /
• pp.4177-4185
• /
• 2012

LED는 환경 친화적인 광원으로 에너지 절약을 위해 기존 조명등을 빠른 속도로 대체하고 있다. 최근에는 에너지 소비가 많은 빌딩의 설계와 옥 내외 광고를 제작함에 있어서 LED를 적극 채택하여 그린 빌딩 및 고효율 저비용 백라이트 도입에 박차를 가하고 있다. 특히 기존의 형광등을 이용했던 실내등과 광고판용 백라이트 분야는 전력소비가 많을 뿐 아니라 SMPS(switched mode power supply)에 의한 부피, 무게 및 수명제한의 제약이 있었다. 그러므로 본 논문에서는 SMPS가 필요 없는 $12{\times}12$ FLB(flexible LED board)와 실내등용 LED 면조명을 위한 AC 직결형 구동기를 개발하였다. 제안 시스템은 고역율의 LID-PC-R101B 칩셋을 포함하고 고효율을 위한 LED 스위치 회로들로 구성되어 있다. 정교한 시스템 디자인을 통해 고효율, 높은 안정성 및 낮은 에너지 소비의 장점을 가지게 한다. 제안된 FLB는 크기 $450{\times}450$ mm, 두께 4 mm 그리고 무게 280 g의 초슬림 구조를 가진다. 최종적으로 제안시스템의 성능검증을 위해 교류 직결형 구동 회로를 채택한 FLB와 면조명의 시제품을 제작하여 실험하였다.

• 미생물학회지
• /
• 제30권2호
• /
• pp.129-133
• /
• 1992

소양호에서 여름에 출현하는 동물 플랑크톤의 포식 작용을 형광 현미경으로 관찰하였다. 형광 bead 와 형광 염색한 세균을 bacterivore 로 보았으며 장내에서 엽록소의 autofluorescence 가 발견되는 것은 algavore 로 보았다. Copepoda 의 유츙과, Thermocyclops, Pleosoma 등은 algavore 였으며, Daphnia, Bosmina, Keratella, Hexathra 등은 bacteriovore 였다. 개체수로는 65.7% 가 algavore 였으며, 34.4% 가 bacteriovore 였다. Bacteriovore 인 동물 플랑크톤은 직경이 0.5 $\mu$m 이상인 입자를 선호하였으며, 형광 bead 와 형광 엽색 세균은 섭취율에 큰차이가 없었다.