• 제목/요약/키워드: Flavonoid and polyphenol

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환삼덩굴 메탄올 추출물의 in vitro 항산화 및 항암 활성 (Antioxidant and Antitumor Activities of Methanolic Extracts from Humulus japonicus)

  • 이연리;김기연;이상훈;김민영;박혜진;정헌상
    • 한국식품영양학회지
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    • 제25권2호
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    • pp.357-361
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    • 2012
  • To evaluate the antioxidant and potential of Humulus japonicus, total polyphenol and flavonoid content, radical scavenging activities, and antitumor activities were measured. The total polyphenol and flavonoid contents of the methanol extracts from Humulus japonicus were $30.13{\pm}1.13$ and $13.61{\pm}0.49$ mg gallic acid equivalent/g extract, respectively. DPPH radical and hydroxyl radical scavenging activities of methanol extracts of Humulus japonicus were 60% and 35%, respectively. The Humulus japonicus higher activities of anticancer activities on liver cancer cell lines compared to other cancer cell lines.

김 분말을 첨가한 쿠키의 품질특성 (Quality Characteristics of Cookies with Added Dried Laver (Porphyra tenera) Powder)

  • 이정애;송정선;윤지영
    • 한국조리학회지
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    • 제23권7호
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    • pp.88-96
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    • 2017
  • This study investigated the quality characteristics of cookies containing laver powder, which is well known for its various functions and biological activity. This study assessed the quality characteristics of cookies containing various concentrations (0, 2, 4, 6 and 8%) of laver powder(LP). To analyze quality characteristics, pH of dough, moisture content, color, hardness, and sensory properties were measured. Qualities, such as pH, brightness, and redness and yellowness and hardness decreased significantly (p<0.001). The total flavonoid and total polyphenol and ABTS radical scavenging activity were proportionally increased with increasing the level of laver powder added in cookies(p<0.001). The antioxidant activity measured by total flavonoid and total polyphenol activities was significantly higher than control and increased proportionally to the laver powder. The result of sensory test shows that sample group adding 2% of laver powder achieved the highest. Based on the above results, this study suggests that the addition of 2% laver powder may be the best substitution ratio for cookies of the improvement of sensory preference and antioxidant. This study exhibited both the functional and health based benefits of laver when it is added to cookies and confirmed the development feasibility of laver cookies considering consumer satisfaction.

생육시기에 따른 양마 잎의 항산화 활성 (Antioxdant Activity of Hibiscus cannabinus L. Leaves in Different Growth Time)

  • 김성무;엄석현;박형재;;유창연;조동하
    • 한국약용작물학회지
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    • 제17권1호
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    • pp.21-25
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    • 2009
  • The results on the useful functional compounds of kenaf (Hibiscus cannabinus L.) leaves cultivated in reclaimed lands and the biological activity effects of extracts were as follows. On 98 days after sowing (DAS) Tainung-2 showed the highest total chlorophyll content (1.68 mg/g), and on 141 DAS Dowling showed higher chlorophyll content (1.50 mg/g) than the other two did. In all cultivars the total chlorophyll content was higher on 141 DAS than on 98 DAS. Total polyphenol and total flavonoid contents were the highest in Tainung-2 (30.50 mg/g and 57.03 mg/g, respectively), and total polyphenol and total flavonoid contents (30.50 mg/g and 57.03 mg/g, respectively) were the highest in 30% ethanol extraction. Ascorbic acid contents were higher on 141 DAS than on 98 DAS in three cultivars. SOD activities of kenaf leaf extract were generally over 90%. DPPH radical scavenging activity of Tainung-2 was higher than others.

음료 제조를 위한 오미자 추출물의 추출 조건에 따른 품질 특성 (Quality Characteristics of Omija (Schizandra chinesis Baillon) Extracts Under Various Conditions for Beverage Production)

  • 민성희
    • 한국식생활문화학회지
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    • 제28권3호
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    • pp.320-327
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    • 2013
  • The quality characteristics of Omija (Schizandra chinesis Baillon) extracts under various extraction temperatures and times were examined. The pH level of the extracts ranged from 3.35-3.47. The sugar and solid content of the samples significantly increased with increasing extraction temperatures and times (p<0.01). In contrast, the lightness of the extracts decreased with increasing extraction temperatures and times (p<0.001). In a palatability test, extracts boiled at $80^{\circ}C$ for 30 minutes scored high in terms of color, flavor, turbidity, sourness, and overall acceptability. Hydroxy radical scavenging activity and polyphenol content of the extracts significantly increased with increasing extraction temperatures and times (p<0.01). Furthermore, direct correlations between hydroxy radical scavenging activity and polyphenol content (or flavonoid content) were established through simple regression (r>0.9) for different extraction temperatures and times. From these results, extracts boiled at $80^{\circ}C$ for 120 minutes is the best to product omija beverage considering functionality and sensory evaluation as well.

인진쑥 추출물 함유 PVA 나노섬유 제조 (Fabrication of Electrospun PVA Nanofibers Loaded with Artemisia capillaris Thunberg Extracts)

  • 김명옥;이정순
    • 한국의류학회지
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    • 제42권2호
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    • pp.269-277
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    • 2018
  • This study extracted Artemisia capillaris Thunberg with distilled water and ethanol to investigate its antioxidant effect. We then investigated the possibility of producing nanofibers by an electrospinning process by adding the extracts to polyvinyl alcohol (PVA). The electrospinning method used a PVA concentration of 12wt.%, an applied voltage of 10kV, and a tip-to-collector distance of 15cm. Total polyphenol and total flavonoid contents were measured to verify the antioxidant activity of Artemisia capillaris Thunberg extracts (ACEs). The total polyphenol content of the distilled water extract and the ethanol extract were measured as 218.47 and 271.26mg/g, respectively, and the total flavonoid content of the distilled water extract and the ethanol extract were measured as 141.68 and 34.98mg/g, respectively. As the content of the ACEs in the PVA nanofibers increased, the Diameters of the nanofibers and the uniformity of the diameters decreased. The electrospinning process was fabricated in a relatively uniform form without beads, and the diameters of the nanofibers that were produced ranged from 340 to 390nm. The results of FT-IR, XRD and DSC analyses confirmed that the ACEs were well mixed with the PVA molecules and were electrospun.

개암 첨가가 청국장의 품질과 항산화 활성에 미치는 영향 (Changes in the Quality Characteristics and Antioxidative Activities of Cheonggukjang prepared Using Hazelnut)

  • 최원석;이난희;최웅규
    • 한국식품영양학회지
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    • 제30권6호
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    • pp.1229-1234
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    • 2017
  • This study was carried out to investigate the changes in the antioxidant activity of Cheonggukjang with the addition of hazelnut. The number of Bacillus subtilis and the content of crude fat were increased with the addition of hazelnut. The addition of hazelnut made the color of Cheonggukjang lighter. The flavonoid and polyphenol content were sharply increased in proportion to the addition of hazelnut. The DPPH radical scavenging activity of Cheonggukjang was also increased with the addition of hazelnut. Taken together, it was found that the addition of hazelnut had a positive influence on Cheonggukjang by increasing the antioxidant activity.

오디에서 열처리와 자외선 조사가 Anthocyanin-Polyphenol Copigment Complex의 안전성에 미치는 효과 (Effects of Temperature and UV Irradiation on Stability of Anthocyanin-Polyphenol Copigment Complex in Mulberry Fruits)

  • 방인수;유창연;임정대
    • 한국약용작물학회지
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    • 제18권3호
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    • pp.191-200
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    • 2010
  • Anthocyanin and polyphenolic compounds present in fruits of mulberry (Morus alba L.) were determined and the influence of temperature and UV irradiation on stability of the anthocyanin-copigment complex were investigated. The copigmentation substance selected in non-anthocyanin fraction from mulberry for the study included: phenolic acid (hydroxybenzoic acid) and flavonoid (quercetin-3-O-$\beta$-D-glucopyranoside). The copigmentation effect increased with the copigment content. UV irradiation had a stronger degradation effect on the copigmentation complex than heating at $80^{\circ}C$. The non-anthocyanin fraction of mulberry and isolated flavonoid (quercetin-3-O-$\beta$-D-glucopyranoside) from mulberry fruit predominated over other copigment substances.

Effects of Physiological Active Substance Extracted from Silkworm Fece

  • Ju, Wan-Taek;Kim, Kee-Young;Sung, Gyoo-Byung;Kim, Yong-Soon
    • International Journal of Industrial Entomology and Biomaterials
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    • 제29권2호
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    • pp.179-184
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    • 2014
  • Silkworm (Bombyx mori ) feces have long been used in the pharmaceutical and food industries as a natural colorant. However, there is limited data on the bioactive compounds that constitute silkworm feces. This research emphasizes the antioxidant activity of different solvent and flavonoid extracts of silkworm feces. The solvents were ethanol, butanol, and water, while the methods utilized included ultrasonification, stirrer, reflux, and reflux after ultrasonification extraction. Results showed that butanol ultrasonification extraction (BUE) yield the lowest extraction (1.75%), while the other methods yielded 7 to 14%. The total polyphenol content utilizing BUE was 3.3 mg TAE/g, while water ultrasonification extraction (WUE) yielded the highest extraction rate with 51.6 mg TAE/g. The total flavonoid content was significantly higher using ethanol reflux extraction (EUE) at 266.8 mg QRE/g BUE, which was 158.3 and 151.3 mg QRE/g. Both DPPH radical scavenging activity and SOD-like (superoxide dismutase) activity, showed significant antioxidant effects. Finally, all other extracts except for BUE had a-glucosidase inhibition at 60%. Therefore, an effective extraction method for physiologically active substances must be selected.

재배조건 및 관수방법에 따른 병풀 (Centella asiatica) 계통의 생육, Asiaticoside 함량과 항산화활성 (Growth Characteristics and Asiaticoside Content, and Antioxidant Activities in Centella asiatica by Cultivation and Irrigation Methods)

  • 최장남;이희정;이윤지;정진태;이정훈;장재기;박춘근
    • 한국약용작물학회지
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    • 제28권4호
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    • pp.254-259
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    • 2020
  • Background: Centella asiatica is a well-known medicinal plant having a wound healing effect. In this study, the growth, asiaticoside content, and antioxidant components and activity were investigated in C. asiatica resources under different cultivation and irrigation conditions. Methods and Results: The cultivation and irrigation methods were divided as greenhouse and open field, and sprinkler and sub irrigation. respectively. Growth characteristics were measured in aerial parts of C. asiatica. Asiaticoside content was analyzed using high performance liquid chromatography. Total polyphenol, total flavonoid and 2,2-diphenyl-1picryl hydrazyl (DPPH) radical scavenging activity were analyzed for antioxidant activities. Growth was higher under greenhouse and sub irrigation than open field and sprinkler conditions. In the leaves, asiaticoside content was 53.45 mg/g (open field) and 34.38 mg/g (sub irrigation), total polyphenol was 41.14 mg/g (open field) and 25.73 mg/g (sub-irrigation), and total flavonoid was 27.26 mg/g (open field) and 23.72 mg/g (sub-irrigation). DPPH radical scavenging activity in the leaves was 85.97 mg·ascorbic acid equivalent (AAE)/g (open field) and 54.83 mg·AAE/g (sub irrigation). Conclusions: Asiaticoside and antioxidant components and activity were not accompanied with high yield, although high growth was observed under greenhouse conditions. Therefore, cultural requirements of C. asiatica should be sufficiently considered to suit each purpose.

Production of Polyphenols and Flavonoids and Anti-Oxidant Effects of Lactic Acid Bacteria of Fermented Deer Antler Extract

  • Kim, Hyun-Kyoung;Choi, Kang-Ju;Ahn, Jong-Ho;Jo, Han-Hyung;Lee, Chang-Soon;Noh, Ji-Ae
    • International journal of advanced smart convergence
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    • 제10권1호
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    • pp.197-208
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    • 2021
  • The deer antler has been used as a major drug in oriental medicine for a long time. Recently, the demand for easy-to-take health functional foods is increasing due to economic development and changes in diet. As part of research on the development of functional materials for antlers, lactic acid fermentation of antler extract was performed. It was intended to develop a functional material with enhanced total polyphenol and flavonoid content and enhanced antioxidant activity. Lactic acid bacteria fermentation was performed by adding 4 types of lactic acid bacteria starter products, B. longum, Lb. Plantarum, Lb. acidophilus and mixture of 8 types of lactic acid bacteria to the antler water extract substrate, respectively. During the fermentation of lactic acid bacteria, the number of proliferation, total polyphenol and total flavonoid content, DPPH radical scavenging and antioxidant activity were quantified and evaluated. As a result of adding these four types of lactic acid bacteria to the antler water extract substrate, the number of lactic acid bacteria measured was 2.04~5.00×107. Meanwhile, a protease (Baciullus amyloliquefaciens culture: Maxazyme NNP DS) was added to the antler extract to decompose the peptide bonds of the contained proteins. Then, these four types of lactic acid bacteria were added and the number of lactic acid bacteria increased to 2.84×107 ~ 2.21×108 as the result of culture. The total polyphenol contents were 4.82~6.26 ㎍/mL in the lactic acid bacteria fermentation extracts, and after the reaction of protease enzyme and lactic fermentation, increased to 14.27~20.58 ㎍/mL. The total flavonoid contents were 1.52~2.21 ㎍/ml in the lactic acid bacteria fermentation extracts, and after the protease reaction and fermentation, increased to 5.59 ~ 8.11 mg/mL. DPPH radical scavenging activities of lactic acid bacteria fermentation extracts was 17.03~22.75%, but after the protease reaction and fermentation, remarkably increased to 32.82~42.90%.