• 한국어류학회지
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• 제32권2호
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• pp.70-77
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• 2020

최초 체중 3.09±0.02 g 조피볼락 치어에서 먹이원으로 냉동크릴 (FWK) 그리고 크릴분말 (KM) 첨가량 (0~40%)에 따라 6종 실험사료로 7주간 사육한 결과 최종 체중, 증체율, 사료효율 및 전어체의 일반성분 중 회분에서는 유의적으로 차이가 나타났지만, 생존율과 전어체의 일반성분 중 수분, 조단백질 및 지질에서는 유의적인 차이 없이 유사하였다. 사육실험 후 크릴 첨가 먹이 종류에 따른 최종 체중은 7.4~12.0 g, 증체율은 141~286%로 먹이 종류에 따라서 유의적으로 성장 차이를 나타내었다. 사료효율은 36~72%로 성장처럼 유사한 경향을 보였다. 크릴을 첨가하지 않은 대조구에서는 12.0 g으로 FWK10구에서 9.3 g과 유의적으로 유사한 최종 체중을 나타내어 다른 시험구보다는 높은 최종 체중을 나타내었다. 즉, FWK 20% 이상 및 크릴분말 0%, 10%, 20%구에서는 7.4~7.8 g으로 유의적으로 낮게 나타났다. 냉동크릴구에서 냉동크릴 무첨가구와 유사하거나 냉동크릴 및 크릴분말 함유량에 따라 유의적으로 낮은 전어체 회분 함량을 나타내었다. 또한 FWK20KM20구에서 어분으로 단백질 함량을 높여주어도 성장, 사료효율 및 전어체의 일반성분에서 차이가 나지 않았다. 이상의 결과로 조피볼락 치어 사료에 냉동크릴 및 크릴분말 10~40% 첨가 시 성장, 사료효율 및 생존율 향상에 효과가 없었다. 그러나 냉동크릴만 10% 첨가 시에는 어분만을 첨가한 사료와 유사한 양호한 성장, 사료효율 및 생존율을 나타내었다.

• Journal of Animal Science and Technology
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• 제57권9호
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• pp.35.1-35.8
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• 2015

Background: An experiment was conducted in Vietnam to test the hypothesis that total dry matter (DM) intake and liveweight (LW) gain would increase in a curvilinear manner with increasing amounts of concentrate offered. Method: There were five treatments: a basal diet of Guinea grass fed at 1 % of LW and rice straw fed ad libitum (T0), or this diet supplemented with concentrate at 0.6 (T1), 1.2 (T2), 1.8 (T3), or 2.4 % of LW (T4). The concentrate comprised locally available ingredients, namely cassava chips, rice bran, crushed rice grain, fishmeal, salt, and urea, mixed manually. Results: Concentrate intake increased from T0 to T3, but there was no difference in concentrate intake between T3 and T4. Total feed intake increased in a curvilinear manner from 4.0 to 6.4 kg DM/d as the quantity of concentrate consumed increased. The substitution of concentrate for grass and rice straw increased with increasing consumption of concentrate and was as high as 0.49 kg DM reduction per kg of concentrate consumed. LW gain increased curvilinearly, with significant differences between T0 (0.092 kg/d), T1 (0.58 kg/d) and T2 (0.79 kg/d); but there were no significant differences in LW gain between T2, T3 (0.83 kg/d) and T4 (0.94 kg/d).With increasing amount of concentrate in the diet, the digestibilities of dry matter, organic matter, crude protein, and crude fat increased, but NDF digestibility decreased. Conclusion: Based on these results, young Vietnamese Brahman-cross growing cattle will respond to a locally-sourced concentrate mix offered at a level of up to 1.2 % of LW.

• 한국식품위생안전성학회지
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• 제31권6호
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• pp.432-438
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• 2016

본 연구는 수산물 중 에톡시퀸 정량시험법을 확립하여 국내 생산 및 수입 양식 수산물에 대한 잔류할 수 있는 동물용의약품인 에톡시퀸에 대한 안전관리 강화기반을 위해 마련되었다. LC-MS/MS를 이용하여 신속하고 효과적으로 정량성 및 정밀성을 확보하였으며, 확립된 시험법의 선택성, 정량한계 및 회수율에 대한 검증을 통하여 에톡시퀸 시험법으로서의 유효성을 확인하였다. 표준용액을 정량한계를 포함한 농도에 따라 검량선을 작성한 결과 $r^2$> 0.99 이상의 직선성을 확인하였으며, 산성용매로 추출 후 MCX 카트리지를 이용해 정제하였다. 본 실험에서의 검출한계는 0.001 mg/kg, 정량한계는 0.01 mg/kg 수준이었고, 평균 회수율은 81.3~107%이었다. 또한, 분석오차는 10% 이하로 정확성 및 재현성이 우수하였으며, CODEX 가이드라인 규정에 만족하는 수준이었다. 따라서, 개발된 시험법은 안전한 국내유통 수산물과 국민보건을 위해 지속적인 잔류실태조사에 활용되고, 수산물 중 잔류동물용의약품의 안전관리에 기여할 것으로 판단된다.

• Fisheries and Aquatic Sciences
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• 제22권10호
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• pp.23.1-23.10
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• 2019

Background: Taurine is a conditional essential amino acid for fish. A study was conducted to investigate the compensating effect of supplemental taurine in diets for red seabream (Pagrus major) on impaired growth performance by fish meal (FM) replacement with soybean meal (SM) at low water temperature (14.15 ± 1.95 ℃). Methods: A FM-based diet was considered as a high FM diet and three other experimental diets were formulated to replace FM with SM by 20, 35, or 50% (HFM, SM20, SM35, or SM50, respectively) without taurine and other four diets were formulated by adding 1% taurine to the diets (HFM-T, SM20-T, SM35-T, or SM50-T, respectively). Triplicate groups of fish (108.9 ± 1.58 g/fish) were distributed into 24 polyvinyl circular tanks (215 L) with 20 fish per tank and fed one of the diets to satiation for 20 weeks. Results: Growth performance and feed utilization of red seabream were significantly improved by the dietary taurine supplementation. SM20-T and SM35-T diets increased fish growth that are comparable to HFM diet. Feed intake, feed conversion ratio, and protein efficiency ratio of fish fed SM20-T and SM35-T diets were not significantly different from those of HFM group. Dietary taurine supplementation in each FM replaced group numerically increased innate immunity of the fish. Lysozyme and superoxide dismutase activities were significantly decreased in fish fed SM35, SM50, and SM50-T diets compared to those of fish fed HFM diet while they were not significantly lower in SM20, SM20-T, SM35, and SM35-T groups. Glutathione peroxidase activity was significantly lower in fish group fed SM50 diet while SM50-T group did not significantly lower compared to that of HFM group. The relative expression level of hepatic IGF-1 mRNA was improved in fish fed taurine-supplemented diets compared to their respective SM diets. Conclusions: Growth performance and feed utilization of red seabream can be accelerated or restored by 1% taurine supplementation when they are fed high level of SM up to 35% in diets during low water temperature season.

• 한국패류학회지
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• 제25권2호
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• pp.121-126
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• 2009

This study was performed to determine the effect of temperature condition on growth of juvenile abalone Haliotis discus hannai with the different feeds. Two types of feed (dry sea tangle and experimental diet) at water temperatures of 20 and $23^{\circ}C$, and 23 and $26^{\circ}C$ were applied to experimental abalone twice. Forty-five juvenile abalone averaging 10.1 g in the first feeding trial and 11.5 g in the second feeding trial were randomly stocked into 6 of 50 L plastic rectangular containers each. Fishmeal, soybean meal and shrimp head meal was used as the primary protein source in the experimental diet. And dextrin and wheat flour, and soybean oil were used as the primary carbohydrate and lipid sources in the experimental diet, respectively. The dry sea tangle and experimental diet were fed to abalone once a day at the ratio of 1.5-2.0% total biomass of abalone with a little leftover in each experimental condition. Weight gain of abalone was significantly affected by feed type, but not by water temperature in the first feeding trial. Regardless of water temperature, weight gain of abalone fed the experimental diet was significantly higher than that of abalone fed the dry sea tangle. However, weight gain of abalone was significantly affected by water temperature, but not by feed type in the second feeding trial. Weight gain was highest in abalone fed the experimental diet at $23^{\circ}C$, followed by abalone fed the dry sea tangle at $23^{\circ}C$, abalone fed the experimental diet and dry sea tangle at $26^{\circ}C$, which was lowest. Moisture and crude protein content of the edible portion of abalone was significantly affected by feed type, but not by water temperature in the second feeding trial. However, ash content of the edible portion of abalone was significantly affected by water temperature, but not by feed type. In considering these results, it can be concluded that the well formulated feed was superior to the dry sea tangle for growth of juvenile abalone, and water temperature conditions of 20 and $23^{\circ}C$ seemed to be better than $26^{\circ}C$ to improve weight gain of abalone.

• Asian-Australasian Journal of Animal Sciences
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• 제17권11호
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• pp.1553-1561
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• 2004

Two feeding trials were conducted with 128 pullets from 4 to 20 weeks of age and 96 laying hens from 23 to 63 weeks of age to evaluate the effects of scavenging and type of protein supplement on the feed intake and performance of improved pullets and laying hens. The experiments had a completely randomized design with four dietary treatments and four replicates. Treatments were: Control (Cont), scavenging but with access to a balanced concentrate at night; confinement (CF) and given the control feed ad libitum; scavenging and supplemented at night with the control feed, but with soybean meal replaced by cassava leaf meal (CLM); scavenging and supplemented at night with the control feed, but with fishmeal replaced y soybean meal (SBM). The mean daily dry matter (DMI), metabolizable energy (MEI) and crude protein intakes (CPI) of the pullets and laying hens, respectively, were 28%, and 18% higher for the confinement treatment (CF) compared to the scavenging treatments (p<0.001). The DMI, MEI and CPI of the pullets were not significantly different among scavenging treatments (p>0.05), but for the layers DMI, MEI and CPI were significantly higher for the CLM and SBM treatments compared to the Cont treatment (p<0.001). In the growing period, the average daily weight gain (ADG), supplement feed conversion ratio (FCR) and supplement feed cost/kg eggs (FCS) were not significantly different for CF compared to Cont, and among scavenging treatments (p>0.05). In the laying period, the hen-day production was significantly lower, and supplement FCR and FCS significantly higher for the CF compared to the scavenging treatments (p<0.001). Egg weight, and yolk, albumen and shell percentage and shape index were not significantly different among the scavenging treatments (p>0.05). However, shell and yolk percentages were significantly lower for the CF compared to the Cont treatment (p<0.01). Mortality was significantly higher for the CF compared to the scavenging treatments for pullets, and was significantly lower for the CF compared to scavenging treatments for laying hens (p<0.001). It was concluded that scavenging pullets and layers were getting around 28% and 18%, respectively, of their nutrient requirements from scavenging activities, resulting in correspondingly lower supplement feed conversion ratios and feed costs. Daily gains of the pullets were not affected by scavenging or protein supplement, but egg production and mortality were lower for the confined hens.

• Asian-Australasian Journal of Animal Sciences
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• 제18권5호
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• pp.671-676
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• 2005

A total of 50 individual catfish, the Tra (Pangasius hypophthalmus) cultivated in either floating cages (Tra-c) or in ponds (Tra-p) and the Basa (Pangasius bocourti) raised in three floating cages, were collected in two of the Mekong Delta provinces. The caudal fin of each individual fish was used for protein electrophoresis employing the SDS-PAGE method. The one fillet sides were used as a representative sample to determine the dry matter (DM), crude protein (CP), ether extract (EE) and amino acids (AAs). The catfish oil was extracted from the belly fats, and the fatty acid (FA) composition was analyzed. There were 21 bands of the Tra and the Basa. Protein bands of the two varieties were 28.6-33.3% polymorphic, while polymorphic individuals of the Tra ranged from 80.0 to 100.0%, and the Basa was 90.0% polymorphic. The phenotypic diversity (Ho) of the Tra ranged from 1.71 to 1.80, while the Basa ranged as high as 2.14%. Diversity values (H$_{EP}$) for genetic diversity markers were equal in the Tra and the Basa. The sum of the effective number of alleles (SENA) of both varieties ranged from 3.40 to 3.83 for the Basa and the Tra, respectively. The lower values of Ho and SENA, as compared with those of the fresh water prawn (Macrobrachium equidens) in the area, would suggest that the species with the low values will become extinct due to inbreeding; the gene pools of each observed population were below a suitable threshold. Many of the differences in the nutritional values of the Tra-c, the Tra-p and the Basa were measured; their nutrient values were comparable to fishmeal or fish oil. Most of the DM, CP, and EE were higher in the Tra, especially in the Tra-c. The essential AA content, especially that of lysine, was highest in the Tra-c, next highest in the Tra-p, and lowest in the Basa. Therefore, the amino acid patterns were closer to the ideal patterns in the same sequences. In contrast, the essential FAs were concentrated in the Basa fish oil. It was found that suitable selection of parents for seed production is required to avoid inbreeding. Catfish may be valuable sources of nutrition for both humans and animals, and the differences in their nutritional values by variety and/or management must be taken into account.

• Asian-Australasian Journal of Animal Sciences
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• 제17권5호
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• pp.621-628
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• 2004

Twenty male buffalo calves of 6-9 months of age (average body weight, 97 kg) were randomly allocated into two main groups of four (control) and sixteen (supplemented) calves. The supplemented group was further divided in to four equal sub-groups, with the two groups supplemented with a liquid preparation of urea-molasses, UML1, containing fish meal and UML2, containing formaldehyde treated deoiled mustard cake (FDMC) and the other two, with a semi-solid preparation, UMC1 with FDMC and deoiled rice bran (DORB) contributing similar level of CP as in UML2 and UMC2 with double the level of FDMC to that in UMC1. The control group was fed with DORB along with ad libitum wheat straw at 40:60 ratios. The rest of the groups were fed on the above diet supplemented with 500 g (as fed basis) of urea-molasses preparations. The experimental feeding was carried out for 24 weeks including a metabolism trial towards the end of experimental feeding. Daily feed intake and fortnightly change in live weight were also recorded during the study. Catalytic supplementation of 500 g urea-molasses induced 8-25% higher voluntary feed intake of wheat straw, resulting in 15-25% higher DM and OM intake. The digestibility of DM, OM, total carbohydrate, NDF, ADF, hemicellulose and cellulose in all the dietary groups were comparable. The CP digestibility of calves in supplemented groups were higher (p<0.05) than the control group. The balance of nutrients, viz. N, Ca and P, was also higher in the supplemented groups. Significantly higher intake of digestible CP coupled with other digestible nutrients attributed to higher TDN (1.67-1.78 vs. 1.37 kg) and ME (5.94-6.31 vs. 4.87 Mcal) intake in urea-molasses supplemented groups which resulted in higher live weight gain compared to that in control group (p<0.01). Between the supplements, UML2 and UMC2 faired non-significantly, indicating formalin treated mustard cake as a suitable replacement to fishmeal in the supplement. The overall ranking based on intake and digestibility of nutrients, live weight gain, economic evaluation and input-output relationship revealed that the rations with UML2 and UMC1 to be of greater value compared to other types. From the study it can be concluded that young ruminants can be reared successfully on a basal diet of deoiled rice bran and wheat straw supplemented with cheaper urea-molasses-mineral mix.

• Asian-Australasian Journal of Animal Sciences
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• 제14권9호
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• pp.1276-1281
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• 2001

The feeding values of four indigenous Kenyan sorghum cultivars and the effects of polyvinylpyrrolidone (PVP) on the utilization of high tannin sorghum by broiler chicks were studied in two 3-week feeding trials. In Experiment 1, one hundred and five broiler chicks (initial average weight 97 g) were randomly assigned to each one of the seven grain-soybean meal diets. The diets consisted of maize [diet 1; no assayable tannin], white sorghum [diet 2; 0.59% catechin equivalents (CE)], cream sorghum [diet 3; 0.94% CE], light brown sorghum [diet 4; 2.71% CE] and dark brown sorghum [diet 5; 3.54% CE]. Diets 6 and 7 were included to test the possibility of overcoming the detrimental effects of sorghum tannins by adding PVP at 0.25% and 0.5% to dark brown sorghum, which resulted in dietary tannin levels of 3.46% and 3.38% CE respectively. In Experiment 2, the effects of tannin on dry matter digestibility (DMD) and nitrogen (N) retention were studied in a 3-week substitution assay in which high tannin sorghum (5% CE) was substituted for white maize at different inclusion levels. Ninety broiler chicks aged 7 days (initial average weight 102 g) were randomly assigned to each one of the six diets. The diets consisted of corn gluten meal and fishmeal as protein sources plus maize [diet 1] and high tannin sorghum at different inclusion levels [diets 2, 3, 4, 5 and 6], resulting in dietary tannin levels of 0, 1.25%, 1.66%, 2.08%, 2.5% and 3.2% CE respectively. Feed intake, feed efficiency and body weight gain were measured weekly. In Experiment 2, tannin absorption, DMD and N retention were measured on days 19, 20 and 21. The results of Experiment 1 showed that feed intake, feed efficiency and body weight gain were all affected by treatment (p<0.05). Diets 1, 2 and 3 gave similar body weight gains and all were better than diets 4 and 5 (i.e. 504, 517, 473 g, vs. 256, 267 g). Similarly, feed efficiencies were higher (p<0.05) for diets 1, 2 and 3 compared to diets 4 and 5 (0.4, 0.42, 0.39 vs. 0.21, 0.23). When 0.25% PVP was added to the dark brown sorghum (diet 6) there was no significant improvement in chick performance (p>0.05). However, addition of 0.5% PVP (diet 7) resulted in significant improvement (p<0.05) in body weight gain compared to the untreated dark brown sorghum. Overall, PVP did not completely overcome the deleterious effects of tannins. The results of Experiment 2 indicate that sorghum inclusion level and subsequent tannin level had no effect on feed intake, feed efficiency, weight gain, DMD and N retention. The above results suggest that tannin level should be limited to below 2.71% CE in broiler chick diets containing 20% CP and 0.4% methionine. However, in diets with 23% CP and 0.8% methionine tannin level of up to 3.2% will not affect performance. Consequently high tannin sorghum (5% CE) can be used to substitute for white maize by up to 100% in broiler chick diets.

• Fisheries and Aquatic Sciences
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• 제19권10호
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• pp.40.1-40.14
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• 2016

Background: Due to the paucity of oceanic resources utilized in the preparation of diets for cultured fish, commercial feed producers have been trying to replace fishmeal (FM) using alternative protein sources such as vegetable protein meals (VMs). One of the main drawbacks of using VMs in fish feed is related to the presence of a variety of anti-nutritional factors, which could trigger an inflammation process in the distal intestine. This reduces the capacity of the enterocytes to absorb nutrients leading to reduced fish growth performances. Methods: We evaluated the mitigating effects of butyrate and taurine used as feed additives on the morphological abnormalities caused by a soybean meal (SBM)-based diet in the distal intestine of sea bass (Dicentrarchus labrax). We used three experimental diets, containing the same low percentage of FM and high percentage of SBM; two diets were supplemented with either 0.2% sodium butyrate or taurine. Histological changes in the intestine of fish were determined by light and transmission electron microscopy. Infiltration of $CD45^+$ leucocytes in the lamina propria and in the submucosa was assessed by immunohistochemistry. We also quantified by One-Step Taqman$^{(R)}$ real-time RT-PCR the messenger RNA (mRNA) abundance of a panel of genes involved in the intestinal mucosa inflammatory response such as $TNF{\alpha}$ (tumor necrosis factor alpha) and interleukins: IL-8, IL-$1{\beta}$, IL-10, and IL-6. Results: Fish that received for 2 months the diet with 30% soy protein (16.7% SBM and 12.8% full-fat soy) developed an inflammation in the distal intestine, as confirmed by histological and immunohistochemistry data. The expression of target genes in the intestine was deeply influenced by the type of fish diet. Fish fed with taurine-supplemented diet displayed the lowest number of mRNA copies of IL-$1{\beta}$, IL-8, and IL-10 genes in comparison to fish fed with control or butyrate-supplemented diets. Dietary butyrate caused an upregulation of the $TNF{\alpha}$ gene transcription. Among the quantified interleukins, IL-6 was the only one to be not influenced by the diet. Conclusions: Histological and gene expression data suggest that butyrate and taurine could have a role in normalizing the intestinal abnormalities caused by the SBM, but the underling mechanisms of action seem different.