• Title/Summary/Keyword: Fishing processes

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An Annealing Control Primer (ACP) System Used for the Isolation and Identification of Copper-Induced Genes in Alfalfa Leaves

  • Lee, Ki-Won;Lee, Sang-Hoon;Kim, Ki-Yong;Ji, Hee Chung;Park, Hyung Soo;Hwang, Tae Young;Choi, Gi Jun;Rahman, Md. Atikur
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.36 no.3
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    • pp.237-242
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    • 2016
  • Copper (Cu) is a necessary microelement for plants. However, high concentrations of Cu are toxic to plants that change the regulation of several stress-induced proteins. In this study, an annealing control primer (ACP) based approach was used to identify differentially expressed Cu-induced genes in alfalfa leaves. Two-week-old alfalfa plants (Medicago sativa L.) were exposed to Cu for 6 h. Total RNAs were isolated from treated and control leaves followed by ACP-based PCR technique. Using GeneFishing ACPs, we obtained several genes those expression levels were induced by Cu. Finally, we identified several genes including UDP-glucuronic acid decarboxylase, transmembrane protein, small heat shock protein, C-type cytochrome biogenesis protein, mitochondrial 2-oxoglutarate, and trans-2,3-enoyl-CoA reductase in alfalfa leaves. These identified genes have putative functions in cellular processes such as cell wall structural rearrangements, transduction, stress tolerance, heme transport, carbon and nitrogen assimilation, and lipid biosynthesis. Response of Cu-induced genes and their identification in alfalfa would be useful for molecular breeding to improve alfalfa with tolerance to heavy metals.

Analysis of the Restoration Actual Conditions on the Quarries II (토석채취지의 복구 실태 분석 II)

  • Park, Jae Hyeon
    • Journal of Korean Society of Forest Science
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    • v.101 no.4
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    • pp.558-566
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    • 2012
  • This study was conducted to offer preliminary data for a natural restoration by the actual condition survey of quarries after extracting rock materials. The mountain restoration area according to a farming and fishing village organize project was restored by green landscape after quarry. However, planting tree species were died due to poor maintenance and administration after restoration works and surface soil loss and erosion occurred during a summer rainfall season because of poor slope greening. It will be needed to review restoration constructions to establish a natural friendly and flawless construction through the selection of optimum restoration works and the examination of soil and geological characteristics. In addition, it is necessary to apply cost-reducing program of restoration constructions. Quarry areas could be restored by the selection of simple and optimum construction works because the most areas were dominant in earth-sand rocks, soft-rocks, and weather-rocks. In addition, the restoration construction project should be examined thoroughly by restoration specialists during green restoration planning and review processes.

Simulation-based Yield-per-recruit Analysis of Chub Mackerel Scomber japonicus in Korean Waters (모의실험을 통한 한국 연근해 고등어(Scomber japonicus)의 가입당 생산 분석)

  • Lee, Kyunghwan;Go, Seonggil;Jung, Sukgeun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.51 no.3
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    • pp.313-320
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    • 2018
  • Chub mackerel Scomber japonicus is an economically important pelagic species in the western North Pacific. In the last 50 years, the annual total catch in Korean waters showed large fluctuations, ranging from 100 to $420{\times}10^3tons$. To provide a biological reference point for management of chub mackerel, we applied a simulation-based yield-per-recruit (Y/R) model that considered both temperature-dependent growth and size-dependent mortality. We estimated the fisheries yield with respect to varying biological reference points and environmental conditions, including 1) the instantaneous rate of fishing mortality (F), 2) length of fish at first capture ($L_c$), and 3) water temperature. The result of our analysis showed that the Y/R could be greatest when the $L_c$ ranges from 19-27 cm and F ranges from $1.48-2.00yr^{-1}$. Y/R increases with increased water temperature between 15 and $23^{\circ}C$. We suggest targeting an $L_c$ of 17 cm (age=0.6 years) under the assumed current of $F=0.48yr^{-1}$ for maximizing the chub mackerel harvest. Further analysis considering spawning and recruitment processes are required to provide biological reference points to ensure the sustainability of chub mackerel fisheries in Korean waters.

Identification of Differentially Expressed Genes in Bovine Follicular Cystic Ovaries

  • Choe, Chang-Yong;Cho, Young-Woo;Kim, Chang-Woon;Son, Dong-Soo;Han, Jae-Hee;Kang, Da-Won
    • The Korean Journal of Physiology and Pharmacology
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    • v.14 no.5
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    • pp.265-272
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    • 2010
  • Follicular cystic ovary (FCO) is one of the most frequently diagnosed ovarian diseases and is a major cause of reproductive failure in mammalian species. However, the mechanism by which FCO is induced remains unclear. Genetic alterations which affect the functioning of many kinds of cells and/or tissues could be present in cystic ovaries. In this study, we performed a comparison analysis of gene expression in order to identify new molecules useful in discrimination of bovine FCO with follicular cystic follicles (FCFs). Normal follicles and FCFs were classified based on their sizes (5 to 10 mm and $\geq25mm$). These follicles had granulosa cell layer and theca interna and the hormone $17{\beta}$-estradiol ($E_2$)/ progesterone ($P_4$) ratio in follicles was greater than one. Perifollicular regions including follicles were used for the preparation of RNA or protein. Differentially expressed genes (DEG) that showed greater than a 2-fold change in expression were screened by the annealing control primer (ACP)-based PCR method using $GeneFishing^{TM}$ DEG kits in bovine normal follicles and FCFs. We identified two DEGs in the FCFs: ribosomal protein L15 (RPL15) and microtubule-associated protein 1B (MAP1B) based on BLAST searches of the NCBI GenBank. Consistent with the ACP analysis, semi-quantitative PCR data and Western blot analyses revealed an up-regulation of RPL15 and a down-regulation of MAP1B in FCFs. These results suggest that RPL15 and MAP1B may be involved in the regulation of pathological processes in bovine FCOs and may help to establish a bovine gene data-base for the discrimination of FCOs from normal ovaries.

Study on a Three-Dimensional Ecosystem Modeling Framework Based on Marine Food Web in the Korean Peninsula (한반도 연근해를 대상으로 해양 먹이망 기반 3차원 생태모델 구축 연구)

  • Cho, Chang-Woo;Song, Yong-Sik;Kim, Changsin;Youn, Seok-Hyun
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.54 no.2
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    • pp.194-207
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    • 2021
  • It is necessary to assess and manage the different elements of the marine ecosystem, such as climate change, habitat, primary and secondary production, energy flow, food web, potential yield, and fishing, to maintain the health of the ecosystem as well as support sustainable development of fishery. We set up an ecosystem model around the Korean peninsula to produce scientific predictions necessary for the assessment and management of marine ecosystems and presented the usability of the model with scenario experiments. We used the Atlantis ecosystem model based on the marine food web; Atlantis is a three-dimensional end-to-end model that includes the information and processes within an entire system, from an abiotic environment to human activity. We input the ecological and biological parameters, such as growth, mortality, spawning, recruitment, and migration, to the Atlantis model via functional groups using existing research and local measurements. During the simulation period (2018-2019), we confirmed that the model reproduced the observed data reasonably and reflected the actual ecosystem characteristics appropriately. We thus identified the usability of a marine ecosystem model with experiments on different environmental change scenarios.

A Study on Changes of Land Use in the Local Port City - Focused on Yeosu in Jeonnam Province - (지방 항구도시의 토지이용 변화에 관한 연구 - 전라남도 여수시를 중심으로 -)

  • Chung, Kumho
    • Journal of the Korean Institute of Rural Architecture
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    • v.25 no.1
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    • pp.9-16
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    • 2023
  • This study is on the formation and the development process of urban space by referring to the literature in Yeosu where is the important location in logistics & transportation and maritime since the Japanese colonial period. There are many factors and results of the development process in Yeosu by the opening port, railroads, loads and industries. The purpose of this study is to understand the characteristics of the formation process of urban space and the characteristics of physical space in Yeosu. The results are as follow; The urban formation and development process in Yeosu where was a small fishing village in the 1910s is largely divided into four processes. Formation: the population increased due to constructions such as of a railroad, a port, and roads and there were many reclamations around the center of the old city center in the Japanese colonial period. Stagnation: There was no urban development due to stagnation, war, and the Yeo-sun Incident. Expand: the industrialization of the Yeocheon Industrial Complex and Gwangyang Steel and other areas around Yeosu led to a surge in Yeosu's population. To cope with this, the city was expanded through three land readjustment projects and the development of large-scale residential complexes. Decline and Remodeling: Yeosu's urban space declined due to the decline of fisheries and the decrease in marine and railway logistics. And the expansion and improvement of the transportation network for hosting the Yeosu World Expo increased the accessibility of the old city center, transforming it into a tourist city using cultural heritage and nature.

A Survey on the Status of Noisy Working Environment in Manufacturing Industries (제조업 산업장의 소음 작업환경 실태에 관한 조사 연구)

  • Kim, Joon-Youn;Kim, Byung-Soo;Lee, Chae-Un;Jun, Jin-Ho;Lee, Jong-Tae;Kim, Jin-Ok
    • Journal of Preventive Medicine and Public Health
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    • v.19 no.1 s.19
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    • pp.16-30
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    • 1986
  • In order to prepare the fundamental data for the improvement of noisy working environments and the effective hearing conservation program on workers exposed to industrial noise, the authors surveyed the working processes and evaluated the noise levels on 56 manufacturing industries in Pusan area from April to July in 1985. The results were summarized as follows : 1. The noise level was the highest in shipbuilding and repairing(95.6 dBA), and followed by steel rolling(94.0 dBA), manufacture of motor vehicles(93.1 dBA), manufacture of fishing nets(92.9 dBA), manufacture of testiles(92.5 dBA), iron and steel foundries(89.3 dBA), manufacture of metal products(89.1 dBA), preserving and processing of marine foods(87.0 dBA), manufacture of rubber products(85.3 dBA), manufacture of plywood(84.9 dBA) and manufacture of paints(84.5 dBA). 2. Among fifty surveyed working processes, the noise level of twenty-one processes (42%) exceeded the threshold limit value for 8 hours per day. 3. As the allowable exposure times by governmental threshold limit values to industrial noise level(dBA), cocking of shipbuilding and repairing and plating(CGL) of steel rolling were the shortest(30 minutes), and followed by assembling(rivet) of manufacture of motor vehicles(1 hour) weaving of manufacture of textiles and shot, machine, pipe laying of shipbuilding and repairing(2 hours). 4. By the result of octave band analysis on noisy working processes in excess of 90 dBA, the sound level was the highest at 2,000 Hz or 4,000 Hz. 5. It was recognized that the measurement of overall sound pressure level was also effective as octave band analysis in evaluating the industrial noise.

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A Comparative Study of Sea Trials and Production Processes for Propulsion Type Working Boats with a Tuna Purse Seiner (다랑어 선망어선 탑재용 보조 작업선의 추진기 형태 변화에 대한 제작과정 및 해상시운전 비교 연구)

  • Ha, Seoung-Mu;Jang, Ho-Yun;Seo, Hyoung-Seock;Seo, Kwan-Cheol
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.23 no.5
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    • pp.595-602
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    • 2017
  • In Korea, much research and development have occurred to enhance the technological competitiveness of tuna purse seining fisheries. Due to these efforts, fishing efficiency has been improved with the development of radar, sonar and global positioning systems for fish detection and revisions to the hull forms of tuna purse seiners. However, for skiff boats, net boats and speed boats, which are auxiliary working boats mounted on tuna purse seiners, technology has lagged behind relative to the modernization of the main vessel. In this study, the hull of an existing propeller-based net boat with steel wire net to protect tuna was changed to the hull of a water jet propulsion vehicle to reduce resistance and improve maneuverability. As a result, a prototype of a water jet propulsion option was produced according to the aluminum structure strength standards specified by the Ministry of Oceans and Fisheries, and safety was confirmed by performing a drop test. Moreover, through a sea trial test, an existing net boat was shown to have a speed of 12.0knots and a towing force of 2,545 kgf at 2,500 RPM. The prototype had a speed of 26.7 knots and a towing force of 2,011 kgf at 3,200 RPM, which satisfied the towing capacity standards of auxiliary working boats mounted on tuna purse seiners.

Effects of climate change on biodiversity and measures for them (생물다양성에 대한 기후변화의 영향과 그 대책)

  • An, Ji Hong;Lim, Chi Hong;Jung, Song Hie;Kim, A Reum;Lee, Chang Seok
    • Journal of Wetlands Research
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    • v.18 no.4
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    • pp.474-480
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    • 2016
  • In this study, formation background of biodiversity and its changes in the process of geologic history, and effects of climate change on biodiversity and human were discussed and the alternatives to reduce the effects of climate change were suggested. Biodiversity is 'the variety of life' and refers collectively to variation at all levels of biological organization. That is, biodiversity encompasses the genes, species and ecosystems and their interactions. It provides the basis for ecosystems and the services on which all people fundamentally depend. Nevertheless, today, biodiversity is increasingly threatened, usually as the result of human activity. Diverse organisms on earth, which are estimated as 10 to 30 million species, are the result of adaptation and evolution to various environments through long history of four billion years since the birth of life. Countlessly many organisms composing biodiversity have specific characteristics, respectively and are interrelated with each other through diverse relationship. Environment of the earth, on which we live, has also created for long years through extensive relationship and interaction of those organisms. We mankind also live through interrelationship with the other organisms as an organism. The man cannot lives without the other organisms around him. Even though so, human beings accelerate mean extinction rate about 1,000 times compared with that of the past for recent several years. We have to conserve biodiversity for plentiful life of our future generation and are responsible for sustainable use of biodiversity. Korea has achieved faster economic growth than any other countries in the world. On the other hand, Korea had hold originally rich biodiversity as it is not only a peninsula country stretched lengthily from north to south but also three sides are surrounded by sea. But they disappeared increasingly in the process of fast economic growth. Korean people have created specific Korean culture by coexistence with nature through a long history of agriculture, forestry, and fishery. But in recent years, the relationship between Korean and nature became far in the processes of introduction of western culture and development of science and technology and specific natural feature born from harmonious combination between nature and culture disappears more and more. Population of Korea is expected to be reduced as contrasted with world population growing continuously. At this time, we need to restore biodiversity damaged in the processes of rapid population growth and economic development in concert with recovery of natural ecosystem due to population decrease. There were grand extinction events of five times since the birth of life on the earth. Modern extinction is very rapid and human activity is major causal factor. In these respects, it is distinguished from the past one. Climate change is real. Biodiversity is very vulnerable to climate change. If organisms did not find a survival method such as 'adaptation through evolution', 'movement to the other place where they can exist', and so on in the changed environment, they would extinct. In this respect, if climate change is continued, biodiversity should be damaged greatly. Furthermore, climate change would also influence on human life and socio-economic environment through change of biodiversity. Therefore, we need to grasp the effects that climate change influences on biodiversity more actively and further to prepare the alternatives to reduce the damage. Change of phenology, change of distribution range including vegetation shift, disharmony of interaction among organisms, reduction of reproduction and growth rates due to odd food chain, degradation of coral reef, and so on are emerged as the effects of climate change on biodiversity. Expansion of infectious disease, reduction of food production, change of cultivation range of crops, change of fishing ground and time, and so on appear as the effects on human. To solve climate change problem, first of all, we need to mitigate climate change by reducing discharge of warming gases. But even though we now stop discharge of warming gases, climate change is expected to be continued for the time being. In this respect, preparing adaptive strategy of climate change can be more realistic. Continuous monitoring to observe the effects of climate change on biodiversity and establishment of monitoring system have to be preceded over all others. Insurance of diverse ecological spaces where biodiversity can establish, assisted migration, and establishment of horizontal network from south to north and vertical one from lowland to upland ecological networks could be recommended as the alternatives to aid adaptation of biodiversity to the changing climate.