• 현장농수산연구지
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• 제7권1호
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• pp.35-46
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• 2005

규산질비료이용 복토직파재배 개선을 위한 폿트시험 연구에서 얻어진 결과를 요약하면 다음과 같다. 본 연구에서 복토재료에 따른 초기 입모율차이는 무복토 55.0%에 비하여 복토처리로 90~100%의 높은 입모율을 보였다. 특히 파종직후 강우조건에서는 무복토 0%의 출아를 보인반면 복토처리에서는 65~100%의 입모율을 보였으며 강우 시에는 무복토 조건에서도 85%의 비교적 높은 입모율을 보였다. 특히 토양을 이용한 복토처리구에서는 강우로 인하여 20%의 매우 낮은 입모율을 보였는데 이는 수분에 의한 토양층 형성으로 산소공급 및 유모출아 조건이 불량하여 입모율이 극히 저조한 것으로 나타났다. 출수기 벼 생육은 무복토(비강우)처리구를 제외한 모든 처리구에서 비교적 양호한 벼 생육을 보였다. 벼 수량은 초기 입모 및 중간생육이 불량한 무복토(비강우) 및 토양 복토구(강우)에서 낮은 수량성을 각각 보였다.

• Fisheries and Aquatic Sciences
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• 제26권3호
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• pp.204-215
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• 2023

The thermolabile haemolysin (tlh) of Vibrio parahaemolyticus (Vptlh) from V. parahaemolyticus is a multiple-function enzyme, initially describes as a haemolytic factor activated by lecithin and phospholipase A2 enzymatic activity (Shinoda, 1991; Vazquez-Morado, 2021; Yanagase et al., 1970). Until now, the tlh structure has hypothesized including N-terminal and C-terminal domain, but what domain of the Vptlh structure does the haemolytic activity has not been refined yet. In this study, a 450-bp VpTLH nucleotide sequence of the entire Vptlh gene encoded the C-terminal domain cloned firstly to examine its responsibility in the activity of the Vptlh. The C-terminal domain fused with a 6-His-tag named the His-tag-VpC-terminal domain was expressed successfully in soluble form in the BL21 (DE3) PlysS cell. Remarkably, both expression and purification results confirmed a high agreement in the molecular weight of the His-tag-VpC-terminal domain was 47 kDa. This work showed the His-tag-VpC-terminal domain lysed the erythrocyte membranes in the blood agar and the phosphate buffered saline (0.9%) media without adding the lecithin substrate of the phospholipase enzyme. Haemolysis occurred at all tested diluted concentrations of His-tag-VpC-terminal domain (p < 0.05), providing evidence for the independent haemolytic activity of the His-tag-VpC-terminal domain. The content of 100 ㎍ of the His-tag-VpC-terminal domain brought the highest haemolytic activity of 80% compared to that in the three remaining contents. Significantly, the His-tag-VpC-terminal domain demonstrated not to involve the phospholipase activity in Luria-Bertani agar supplemented with 1% (vol/vol) egg yolk emulsion. All results proved the vital responsibility of the His-tag-VpC-terminal domain in causing the haemolytic activity without the required activation by the phospholipase enzyme. Raw extracts of Phellinus igniarus and Phellinus pipi at 10^-1 mg/mL inhibited the haemolytic activity of the His-tag-VpC-terminal domain from 67.7% to 87.42%, respectively. Hence applying the His-tag-VpC-terminal domain as a simple biological material to evaluate quickly potential derivatives against the Vptlh in vivo conditions will accessible and more advantageous than using the whole of the Vptlh.

• 한국양식학회:학술대회논문집
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• 한국양식학회 2003년도 추계학술발표대회 논문요약집
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• pp.67-67
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• 2003

Dorsal aorta cannulated rainbow trout averaging 505 $\pm$ 6.5g (Mean $\pm$ SD) were divided into 7 groups (5 fish per group). Twenty four hour post feeding, cannulated fish were intubated with one of seven L-amino acid diets containing graded levels of Valine (Val. : 0.45, 0.95, 1.20, 1.45, 1.70, 1.95 or 2.45%) at 1% of body weight. Blood samples were taken at 0, 5 and 24hr after feeding the experimental diets. Post-prandial (5h after feeding) plasma-free Valine concentrations (PPval.) increased linearly from fish fed diets containing Valine between 0.45 and 1.45%, but the concentration remained constant from fish fed diets containing valine between 1.45 and 2.45%. Post-absorptive (24h after feeding) plasma free valine concentrations (PAval) increased linearly from fish fed diets containing valine between 0.45 and 1.45%, but the concentration remained constant from fish fed diets containing valine between 1.45 and 1.95%. Using the broken-line model analysis, the dietary valine requirement by PPval and PAval could be 1.44 and 1.50% in rainbow trout, respectively. These results supported that the estimated dietary valine requirement by PP(val.) and PA(val.) could be in close agreement with the values obtained from the previous conventional feeding method. Thus, the use of PFAA concentrations for determining essential amino acid requirements could be possible in rainbow trout with dorsal aorta cannulation.

• 해양환경안전학회지
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• 제19권2호
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• pp.186-192
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• 2013

황해(Yellow Sea)는 평균수심(44 m)이 낮고 한국과 중국연안에 넓은 갯벌이 있어서 1차 생산성(>300 $gC/m^2/year$)이 매우 높아 수산자원 등 해양생물자원이 풍부한 생태물리환경을 지니고 있다. 그러나 황해는 중국대륙과 한반도로 둘러싸인 반폐쇄해역(49만$km^2$)의 광역해양생태계로서 오염 및 남획 등에 의한 외부충격이 심할 경우 생태계의 균형이 파괴되고 회복이 불가능한 특성을 지니고 있다. 최근 한 중간 어업협정 이후 중국 어민의 남획 및 불법조업 그리고 한 중 연안의 과도한 개발로 인한 환경피해로 황해생태계에 심각한 충격이 가해지고 있다. 본 논문은 한 중 황해거버넌스의 주요 구성요소인 이해당사자, 법률 및 제도, 정부조직 및 정책을 고찰하고 한 중 황해국제협력인 황해광역생태계보전사업(YSLME), 북서태평양보전실천계획(NOWPAP), 한 중황해환경공동조사사업의 평가를 통해 황해의 이용 및 개발압력의 지속적 증가에 대처하는 통합적 거버넌스체계의 구축을 제안하고 있다.

• 한국양식학회지
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• 제18권3호
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• pp.173-179
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• 2005

Superoxide dismutase (SOD), an antioxidant enzyme catalyzing the first step for scavenging the reactive oxygen species is important as an early warning indicator to address various biological stresses. For this reason, the monitoring the expressed pattern of SOD gene in fish organs is one of important biomarkers to assess the aquatic pollution caused by many toxic chemicals. Based on the Northern blot hybridization, semi-quantitative and/or realtime RT-PCRs, the alteration of SOD gene transcripts in shark liver was examined during the experimental acute exposures to cadmium. The expression of SOD at mRNA level was up-regulated both by injection (0, 0.5, 1 or 2 mg $CdCl_2/kg$ body weight for 48 hours) and by immersion (0 or $5{\mu}M$ Cd for 0, 1, 4 and 7 days) treatments of cadmium. The transcriptional stimulation of shark SOD gene by cadmium exposure was dependent upon doses and durations: there was a trend toward more increase in higher dose and longer durations of exposure. The hepatic SOD mRNA levels showed also a general agreement with the tissue cadmium concentrations accumulated in immersion exposure. This result may provide useful strategy to develop a fine molecular biomarker at mRNA level for detecting aquatic pollution caused by toxic metals.

• 통상정보연구
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• 제17권1호
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• pp.309-337
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• 2015

2014년 11월 한 중 양국 정부는 한 중 자유무역협정(FTA)을 실질적으로 타결하였다. 한 중 FTA에서 중국은 품목수 기준 90.7%(7,428개), 수입액 기준 85%(1,417억 달러)에 해당하는 품목의 관세를 최장 20년 내에 철폐하기로 한 반면 한국은 20년 내에 품목수의 92.2%(1만 1,272개), 수입액의 91.2%(736억 달러)를 철폐하기로 하였다. 한 중 FTA 타결로 우리나라는 미국, EU, 중국, 즉 세계 3대 경제권과 모두 FTA를 체결함으로써 글로벌 FTA 네트워크를 완성하게 되었다. 본 연구는 한 중 FTA의 파급효과가 지역 및 산업간 다를 수 있다는 전제 하에 한 중 FTA가 '대구 경북'라는 특정 지역의 주요 핵심 산업에 어떤 영향을 줄 것인지를 분석하였다. 본 연구에서는 대구 경북 지역의 대중 교역 현황, 한중간 관세율 비교, 지역 핵심산업의 무역특화지수, 그리고 한 중 FTA 협상결과를 분석함으로써 한 중 FTA가 대구 경북 지역의 주력 핵심 산업 - 섬유, 전자 전기, 기계 및 자동차, 철강 금속 산업에 미치는 영향을 연구하였다.

• 한국항해항만학회지
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• 제27권4호
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• pp.431-436
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• 2003

국제적으로는 한ㆍ일, 한ㆍ중 어업협정의 체결로 인한 주변국과의 어업협상력 제고 및 국내적으로는 IMF 체제를 겪으면서 어업경영에 있어서 어업의 존폐위기를 몰고오는등 국내외 사정이 무엇인가 일대 혁신을 요구하는 새로운 대안을 모색하는 단계에 이르렀다. 해양수산부에서는 어업분양의 선진화를 위하여 어로활동의 현황파악 및 문제점을 실시간으로 분석하는 어업정보화의 발전방향을 설정하여 해양자원관리 및 활용성 제고를 통한 종합적이고 체계적인 정보화 기반을 조성하고 있다. 어업통신의 정보화는 전선화되는 자료가 원격지 여부에 관계없이 필요한 사용자에게 공유될 수 있는 환경을 구축하는 것으로 전산화-정보통신망-이용자 서비스까지를 종합적으로 고려하여 구축하여양 한다. 해상이동체인 어선들을 상대로 정보통신망을 구성하기 위한 최적의 방안으로 기존의 SSB 통신시스템을 이용하는 방법을 연구하였다. 본 연구에서는 운용비의 부담이 없는 HF SSB통신에 의한 어선의 어업통신망의 구축을 위하여 어업정보화의 환경, 디지털 어업정보 통신망의 구성 및 무선모뎀에 대하여 제안하였다.

• 대한수의학회지
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• 제35권3호
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• pp.583-593
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• 1995

The present study was conducted to develop a toxoplasma latex agglutination test antigen(Test-MT) and evaluate the toxoplasma latex agglutination(LA) test using a newly-made "Test-MT kit" by comparing with the Toxo-MT kit(Eiken chemical co, Tokyo). Also, the specifity and sensitivity test were made by comparing with IFA test and IgG-ELISA. Tachyzoite suspensions of Toxoplasma gondii(RH strain) were ultracentrifuged for 30min at $60,000{\times}g(4^{\circ}C)$ and the supernatant was used as a water-lysate antigen. Polystyrene latex particles of $1.0{\mu}m$ in diameter(Polyscience co) were used for the preparation of sensitized latex-antigen supension(Test-MT). The frequency distribution of LA titers in Test-MT showed two peaks at <1:32 and 1:128. The borderline titer for positive test in Test-MT was determined to be 1:64. But the frequency distribution of LA tites in Toxo-MT showed two peaks at <1:16 and 1:64. The positive borderline was determined to be 1:32. Agreement of reactions between Test-MT and Toxo-MT kit by LA test was shown 92.5% in bovine sera and 97.0% in swine sera, respectively. From the results obtained here it was determined that the sensitized latex-antigen, Test-MT kit, for the microtiter agglutination test prepared as same as by the procedure described in the previous paper(Suh and Lee, 1993) was useful as a highly specific, sensitive and stable immunotiteration reagent for serodiagnosis of toxoplasma infection in animal sera.

• 대한지역사회영양학회지
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• 제27권6호
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• pp.480-491
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• 2022

Objectives: This study examined the problems encountered when analyzing the market size, purchase, and consumption of HMR (home meal replacements) in the Republic of Korea. Methods: The macro data relevant to the market size and purchase status of HMR were critically summarized. The micro data retrieved from the 2019 & 2020 Korea National Health and Nutrition Examination Survey (KNHANES) were analyzed to understand the consumption of HMR. Results: The Korea Agro-Fisheries & Food Trade Corporation and the Ministry of Food and Drug Administration reported the market size of HMR, whereas the Korean Rural Economic Institute and the Rural Development Administration reported the purchase expense and frequencies of HMR. Since the values on the market size and purchase status were calculated or surveyed using different scopes of HMR, there have been reliability issues for the data presented. Additionally, lack of consensus on the use of Korean terms corresponding to HMR was found to be a problem. To examine the consumption of HMR, analysis of the food intake data from KNHANES presented results with very low validity due to the inappropriate survey and coding scheme not reflecting the inclusion of new food types. Conclusions: Several problematic discrepancies were encountered in the statistics on HMR. The fundamental cause of these problems was the absence of agreement on the scope of HMR and the Korean terms corresponding to it. Considering the increasing importance of HMR in Korean diets, urgent cooperative efforts are required between the government and academia to derive an agreed Korean term and establish the scope of HMR.

• Ocean and Polar Research
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• 제45권2호
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• pp.57-69
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• 2023

Growth responses along the gradient of water temperature, salinity, and light intensity and cytotoxicity against Artemia nauplii were explored using Ostreopsis sp. and Coolia canariensis strains, representing the two potentially toxic epiphytic dinoflagellate (EPD) species from Jeju coastal waters of Korea. Variation in maximum growth rate (GRmax) and maximum biomass yield (Ymax) along the environmental gradients was quite contrasting between the two strains, which appears to be reflected in the in situ abundance distribution of the corresponding genera. The more eurythermal characteristics of Ostreopsis sp. strain were in good agreement with the relative distribution of Ostreopsis spp. and Coolia spp. in 520 macroalgal samples collected from 6 stations. The more stenohaline C. canariensis strain was well matched by a markedly narrower range of salinities in the in situ distribution of Coolia spp. than the salinity range for Ostreopsis species. The differences in light adaptation between the high light-preferring Ostreopsis sp. strain and the more euryphotic C. canariensis strain were remarkably consistent with the distinct vertical profiles of Ostreopsis spp. and Coolia spp. abundance in the red alga Amphiroa sp. off Moom-seom. Cytotoxicity against Artemia nauplii in the Ostreopsis sp. preparation with 1000 cells ml^-1 was similar to that in C. canariensis preparation with 12000 cells ml^-1, which is noteworthy. Thus, the new potential cytotoxicity risks from C. canariensis along with the well-known toxic genus Ostreopsis may be introduced to Jeju coasts, which necessitates further exploration into the contrasting ecological niches occupied by EPD species in relation to their cytotoxicity.