• 제목/요약/키워드: Fish-frame

검색결과 109건 처리시간 0.024초

Identification and Expression of Retroviral Envelope Polyprotein in the Dogfish Squalus mitsukurii

  • Kim, Soo Cheol;Sumi, Kanij Rukshana;Choe, Myeong Rak;Kho, Kang Hee
    • 한국해양생명과학회지
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    • 제1권2호
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    • pp.88-94
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    • 2016
  • Determining the infection history of living organisms is essential for understanding the evolution of infection agents with their host, particularly for key aspects such as immunity. Viruses, which can spread between individuals and often cause disease, have been widely examined. The increasing availability of fish genome sequences has provided specific insights into the diversity and host distribution of retroviruses in fish. The shortspine spurdog (Squalus mitsukurii) is an important elasmobranch species; this medium-sized dogfish typically lives at depths of 100~500 m. However, the retroviral envelope polyprotein in dogfish has not been examined. Thus, the aim of the present study was to identify and analyze the retroviral envelope polyprotein in various tissues of dogfish. The 1334-base pair full-length novel cDNA of dogfish envelope polyprotein (dEnv) was obtained by 3' and 5'-rapid amplification of cDNA end analysis from S. mitsukurii. The open reading frame showed a complete coding sequence of 815 base pairs with a deduced peptide sequence of 183 amino acids that exhibited 34~50% identity with other fish and bird species. It was also expressed according to reverse transcription and real-time polymerase chain reaction in the kidney, liver, intestine, and lung, but not in the gill. This distribution can be assessed by identifying and analyzing endogenous retroviruses in fish, which consists of three main genes: gag, pol and env. Dogfish envelope polyprotein sequence is likely important in evolution and induces rearrangements, altering the regulatory and coding sequences. This is the first report of the identification and molecular characterization of retroviral envelope polyprotein in various tissues of S. mitsukurii.

역사극의 분석틀을 활용한 영화 <자산어보>의 내레이션 특성에 관한 연구 (A Study on the Narration Characteristics of <The Book of Fish> Using the Analysis Frame of Historical Drama)

  • 채희상
    • 문화기술의 융합
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    • 제9권4호
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    • pp.351-356
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    • 2023
  • 본 연구는 영화 <자산어보>(2021)가 19세기 성리학적 질서가 흔들리며 서서히 몰락해가는 조선을 어떤 방식으로 재현하고 있는지를 분석을 통해 살펴보기 위해 기획되었다. 분석에 앞서 역사극의 내레이션 특성을 고려하여 역사극의 분석틀을 제시하였다. 우리는 역사극의 분석틀을 활용하여 <자산어보>가 유배 서사의 플롯 구조를 기반으로 정약전, 장창대가 시대의 한계와 가능성 사이에서 주체적 개인으로 자신의 삶을 살아가는 모습을 재현하고 있음을 확인하였다. 흑백과 컬러 이미지의 대비, 보이스 오버 내레이션의 적극적 활용, 한시 자막과 음악 등으로 만들어낸 중심기억과 잉여기억을 통해 영화는 주체적 개인으로 살아가기 위해 필요한 것이 무엇인지에 관한 보편적인 질문을 우리에게 던지고 있다.

한국에서 분리된 전염성 조혈괴저 바이러스의 non-virion (NV) 단백질의 유전자 클로닝 및 바이러스 증식에서의 역할 (Cloning of the non-virion (NV) of a Korean Isolate of Infectious Hematopoietic Necrosis and Identification of the Role of the NV in IHNV Replication)

  • 문창훈;조화자;윤원준;박정재;박정민;김현주;도정완;이주양;임채렬
    • 미생물학회지
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    • 제36권2호
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    • pp.103-108
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    • 2000
  • 한국에서 분리된 전염성 조혈괴저바이러스(infectious hematopoietic necrosis virus, IHNV)인 IHNV-PRT의 non-viron(NV)단백질을 암호화하고 있는 cDNA를 클로닝하여 이들의 염기서열을 분석하였다. NV는 336bpzmrl의 open reading frame을 포함하였으며 이로부터 111개의 아미노산 서열을 외국에서 분리된 IHNV들과 비교 분석한 결과 90-95%의 상동성을 보였다. 이러한 사실은 INHV의 NV단백질 유전자들은 IHNV의 strain에 관계없이 매우 보존되어 있음을 나타내준다. Northern blotting을 사용하여 NV의 발현을 측정한 결과 감염 후 20 시간분터 발현이 증가함을 확인 힐수 있었다. NV가 바이러스의 증식에 필요한지의 여부를 확인하기 위하여 바이러스 유전자의 antisense DNA를 사용하여 바이러스 증식 억제에 관한 실험을 수행하였다. Glycoprotein (G)의 antisense DNA를 처리한 경우 바이러스의 증식이 거의 억제된 반면 NV에 대한 antisense DNA를 처리한 경우 바이러스 증식에 거의 변화가 없었다. 이로부터 배양중인 세포가 있어서 NV는 증식에 필수적이지 않은 것으로 판단된다.

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파랑 및 흐름중 모형 가두리 시설의 운동 특성 (Dynamic Motions of Model Fish Cage Systems under the Conditions of Waves and Current)

  • 김태호;김재오;류청로
    • 한국수산과학회지
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    • 제34권1호
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    • pp.43-50
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    • 2001
  • 규칙파, 불규칙파 및 파랑과 흐름이 공존하는 상태에서 프레임에 우리형 그물을 부착하여 구성되어 있는 가두리 시설의 운동특성을 분석하기 위하여 정사각형 및 원형 구조의 가두리 시설을 대상으로 예인 수조에서 수리 모형실험을 실시하였으며, 규칙파중 운동 특성은 선형 포텐셜 이론에 의한 수치 해석의 그것과 비교하였다. 그 결과는 다음과 같이 요약할 수 있다. 1) 규칙파중 모형 가두리 시설의 상하 및 종 동요는 전후 동요와는 달리 그물의 영향을 거의 받지 않았으므로 이 시설물의운동 특성중 가장 중요한 상하 동요를 해석하는 경우에는 그물을 제외하고 프레임만을 고려해도 된다는 것을 확인하였다. 2) 불규칙파중 및 파랑과 흐름이 공존하는 상태에서 모형 가두리 시설의 운동 특성은 입사파 주기의 2배 되는 고주파수에서 시설물의 고유 주기 등에 의해 동적 운동의 peak frequency가 나타남으로써 비선형 즉, 2nd order harmonic 성분이 존재하였다 3) 파동 및 흐름장에서 동요 특성이 정사각형에 비해 비교적 작게 나타난 원형이 파도, 조류 등 환경 조건이 거친 해역에 보다 적합한 것으로 판단되었다.

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연어 frame 유래 근육을 이용한 연어 패티의 제조 및 특성 (Preparation and Characterization of Salmon Patty using Muscle from Salmon Frame)

  • 허민수;김진수
    • 한국수산과학회지
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    • 제42권3호
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    • pp.183-189
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    • 2009
  • This study was conducted to prepare salmon patty using muscle separated from salmon frame (SPFM) and to investigate the food component characterization. When compared to salmon patty with fillet muscle (SPM), SPFM was lower in the moisture content, while it was higher in crude lipid content. However, no differences in the ash and protein contents between SPFM and SPM were found. Compared to SPM, the Hunter color value in cross section of cooked SPFM was higher in a and $\Delta$E values, while the color was lower in Land b values. Trichloroacetic acid soluble-N content of SPFM was 279 mg/100 g, which was insignificantly different (P>0.05) compared to those of SPM and commercial patty. The hardness of SPFM was 0.44 kg/$cm^2$, which was insignificantly different (P>0.05) compared to that of SPM, while was higher than that of commercial patty. The major fatty acids of SPFM were 16:0 (16.5%), 18:1n-9 (29.2%) and 18:2n-6 (26.1%). The 20:5n-3 and 22:6n-3 were also detected in high composition. The total amino acid content of SPFM was 16.6 g/100 g, which was similar to that of SPM. However, the total amino acid of SPFM was 14% higher than that of commercial patty. From the results of the mineral content, SPFM was higher than that of SPM in Fe and Ca, while the K in SPFM was lower. According to the result of sensory evaluation on the color, flavor and taste, no significant differences in all sensory items between SPFM and SPM were found.

Cloning and Characterization of DAP10 homologue gene from Olive Flounder, Paralichthys olivaceus

  • 박찬일;김무찬;황지윤;김기혁;김주원
    • 한국어병학회지
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    • 제19권3호
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    • pp.227-233
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    • 2006
  • Olive flounder immunoreceptor DAP10 homologue cDNA was cloned from a peripheral blood lymphocytes (PBLs) cDNA library. The length of the olive flounder DAP10 cDNA is 473bp and it contains an open reading frame of 234bp. The predicted polypeptide sequence is 78 amino acids, consisting of a 22-amino acid leader, an 11-amino acid extracellular domain, a 21-amino acid transmembrane segment, and a 24-amino acid cytoplasmic domain. The amino acid sequence of olive flounder DAP10 has 56%, 50%, 32%, 31%, and 31% sequence identity with zebrafish DAP10, catfish DAP10, cattle DAP10, rat DAP10 and Monkey DAP10, respectively. Olive flounder DAP10 has a conserved aspartic acid in the transmembrane domain and a phophatidylinositol-3 kinase-binding site (YxxM/V) in the cytoplasmic region. Genomic organization reveals that olive flounder DAP10 comprises five exons and four introns. A phylogenetic analysis based on the deduced amino acid sequence grouped the olive flounder DAP10 with other species DAP10. In RT-PCR analysis, DAP10 transcripts were detected predominantly in PBLs, kidney, spleen and intestine.

Molecular Cloning of Estrogen Receptor $\alpha$ in the Masu Salmon, Oncorhynchus masou

  • Sohn, Young Chang
    • 한국양식학회지
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    • 제17권1호
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    • pp.62-68
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    • 2004
  • A cDNA encoding the masu salmon, Oncorhynchus masou, estrogen receptor $\alpha$ (msER$\alpha$) was cloned from the pituitary gland by polymerase chain reaction (PCR). This cDNA contains an open reading frame encoding 513 amino acid residues, and the calculated molecular weight of this protein is about 56,430 Dalton. The amino acid sequences of the DNA binding and ligand binding domains of msER$\alpha$ showed high homology to those of other fish species (84-100%). Reverse transcription PCR analysis showed that the mRNA level of msER$\alpha$ in the pituitary was slightly higher in estradiol-17$\beta$(E2) injected masu salmon than that of control fish. To test the biological activity of msER$\alpha$, the cDNA was ligated to a mammalian expression vector and transfected into a gonadotrope-derived cell line, L$\beta$T2, with a reporter plasmid including estrogen responsive element. Expression of the reporter protein, luciferase, was E2 and msER$\alpha$-dependent. The masu salmon ER$\alpha$ is structurally conserved among teleost species and functions as a transcriptional activator in the pituitary cells.

열구자탕(悅口子湯)의 문헌적 고찰 (A bibliographical study of Yeolgujatang)

  • 송혜림;이효지
    • 한국식생활문화학회지
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    • 제18권6호
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    • pp.491-505
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    • 2003
  • Yeolgujatang is traditional casserole with meat, fish and vegetables in special pot. Name of Yeolgujatang was in 1800s, as yeolgujatang, yeolgujatangbang, yeolguja, in 1900s, Yeolgujatang, royal feast food in Yi Dynasty was yeolgujatang, Meon-sinseolro, tangsinseolro. Yeolgujatang frame has legs and a lid, and a cylinder that can contain charcoal at the center of its rounded pot. with this charcoal, food can be cooked. Its material has changed from brazier to brassware, stainless steel, and silver. Nowdays electric sinseolro was also launched, which uses electric power instead of charcoal. Materials in yeolgujatang are beef, intestines, pork, chicken, pheasant, fish, sea bream, abalone, shrimps, vegetables, mushroom, ddock, guksu, cooked rice, seasening and garnish. Nutrition of Yeolgujatang per capita contains 221.5kal of calory, 17.3g of protein, 16.5g of fat, 6.1g of carbobydrates, 2g of fiber, 57.6mg of calcium, 208mg of phosphorus, 4.3mg of ferrum, $2177{\mu}gRE$ of vitaminA, 1.58mg of vitamin $B_1$, 0.3mg vitamin $B_2$, 6.6mg of vitaminC and 5.26mgNE of niacin. Yeolgujatang is excellent in nutrition, except for calcium and vitaminC.

Molecular identification and expression analysis of a natural killer enhancing factor-A from black rockfish Sebastes schlegelii

  • Lee, Jeong-Ho;Kim, Joo-Won;Park, Chan-Il
    • 한국어병학회지
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    • 제22권3호
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    • pp.343-352
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    • 2009
  • Natural-killer-cell-enhancing factor (NKEF) belongs to the newly defined peroxiredoxin (Prx) family. It was originally isolated from human erythroid cells. The black rockfish NKEF cDNA was identified through the expressed sequence tag (EST) analysis of PBLs libraries. The full-length NKEF cDNA was 1433 bp long and contained an open reading frame (ORF) of 594 bp that encoded 198 amino-acid residues. The 5' UTR had a length of 39 bp, and the 3’UTR 800 bp. The deduced amino-acid sequence of the black rockfish had a density 93.4, 92.9, 87.8, 85.8, 84.8, 83.8, 80.3, 79.7, 77.2, and 75.2% that of the pufferfish, olive flounder, channel catfish, zebrafish, chicken, common carp, Myotis lucifugus, cattle, human PrxI, rat PrxI, human NKEF-A, and Xenopus tropicalis, respectively. The NKEF gene was expressed in all the tissues of the black rockfish. The RT-PCR indicated that the NKEF transcripts were predominantly in the spleen and gill, less dominantly in the PBLs, head kidney, trunk kidney, and liver, and least in the intestine and muscles. This is the first report on the existence of the NKEF-A gene in black rockfish.

Characterization of histone gene expression in sevenband grouper, Hyporthodus septemfasciatus against nervous necrosis virus infection

  • Lee, Dong-Ryun;Lee, A-Reum;Krishnan, Rahul;Jang, Yo-Seb;Oh, Myung-Joo;Kim, Jong-Oh
    • 한국어병학회지
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    • 제35권1호
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    • pp.121-128
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    • 2022
  • Recent studies revealed that histone proteins are involved in innate immune responses during pathogen invasion as well as DNA packing. This study characterized the histone genes (H2A.V) of sevenband groupers and analyzed gene expression in NNV-infected sevenband groupers. The open reading frame (ORF) of H2A.V is 387 bp which encoded 128 amino acid residues. The deduced amino acid sequence of H2A.V harbor a highly conserved domain for H2A/H2B/H3 and H2A_C binding domain. Quantitative real-time PCR analysis showed that H2A.V had a high gene expression level in the brain and blood after being NNV-infected. An increase in extracellular histone protein in the blood has been identified as a biomarker for vascular function in humans. More research is required to understand histone's immune response at the protein level or in aquatic animals.