• Journal of Life Science
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• v.11 no.1
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• pp.76-82
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• 2001

Fifteen species of microorganisms were isolate form the intestines of fishes, fish feed, and ferment. Eleven microorganisms except HY4, HY8, HY12, and HY13 were Gram-positive, and HY1, HY2, HY3, HY5, HY6, and HY7 produced lactic acid. The species of HY1, HY2, HY3, HY4, HY5, HY6, HY13, and HY14 showed some growth in the medium containing 1% of NaCl. Except HY6, HY7, HY8, HY12 and HY5, 10 isolates had proteolytic activity, whereas only HY13 and HY14 had lipase activity. From all the results four isolates (HY3, HY4, HY13 and HY14) were chosen for the degradation of fish wastes. There was no mutual inhibition among the microorganisms, and the optimum temperature and pH for the growth of the mixed culture were found to be 3 2$\^{C}$ and 7, respectively. Under the optimum growth conditions the maximum optical density and the maximum specific growth rate were estimated to be 2.35 and {TEX}$0.46h^{-1}${/TEX}, respectively. Major microorganisms in the mixed culture at the log-phase were HY3 and HY4, which occupied 70%. The degrading efficiency of fish waste by the mixed microorganisms was 2.3 times higher, compared to control. The total amount of free amino acids in the degraded products from fish wastes was 39g/100g protein and little odor was produced by the mixed microorganisms after 48 hours.

• Journal of Microbiology and Biotechnology
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• v.32 no.1
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• pp.64-71
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• 2022

The discarding of wastes into the environment is a significant problem for many communities. Still, food waste can be used for lactic acid bacteria (LAB) growth. Here, we evaluated three growth media equivalent to de Mann Rogosa Sharpe (MRS), using apple bagasse, yeast waste, fish flour, forage oats, and cheese whey. Cell-free supernatants of eight LAB strains were tested for antimicrobial activity against nine indicator microorganisms. The supernatants were also evaluated for protein content, reducing sugars, pH, and lactic acid concentration. Cell-free supernatants from fish flour broth (FFB) LAB growth were the most effective. The strain Leuconostoc mesenteroides PIM5 presented the best activity in all media. L. mesenteroides CAL14 completely inhibited L. monocytogenes and strongly inhibited Bacillus cereus (91.1%). The strain L. mesenteroides PIM5 consumed more proteins (77.42%) and reducing sugars (56.08%) in FFB than in MRS broth (51.78% and 30.58%, respectively). Culture media formulated with agroindustrial wastes positively improved the antimicrobial activity of selected LAB, probably due to the production of antimicrobial peptides or bacteriocins.

• Journal of environmental and Sanitary engineering
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• v.16 no.1
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• pp.108-116
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• 2001

It is difficult to task to achieve high biological nutrient removal from municipal wastewater because of low organic content. Volatile fatty acids(VFAs) produced from acid fermentation of food wastes can be utilized as external carbon sources for the biological nutrient process. Significant reduction and stabilization of the food wastes can also be obtained from the acid fermentation. The objective of this study is to evaluate characteristics of acid fermentation of the food wastes. Results obtained from the batch experiment of various organic wastes showed that the food wastes had high potential to be used as an external carbon source because of the largest production of the VFAs with low nitrogen and phosphorus content. The fish waste was found to be the next possible organic waste, while the others such as radish cabbage and molasses waste showed high VFAs consumption potential as a results of high nitrogen and phosphorus content. alkaline hydrolysis of the food waste was carried out using NaOH prior to the acid fermentation. As the alkali addition increased, solubilization of the organics as well as TSS reduction increased. However, fraction of soluble COD to total COD became stable after a sharp increase. Alkali addition greater than 0.5g NaOH per g TS resulted in significant increase in pH.

• Fisheries and Aquatic Sciences
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• v.14 no.3
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• pp.230-233
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• 2011

To determine the cost-effectiveness of converting fish waste into liquid fertilizer, this study analyzed the production of 3 L of liquid fertilizer from the fermentation of fish waste. The total product cost of the fertilizer was calculated to be $165.26 for a one-batch operation. If the seed culture was repeated five times, the total product cost could be reduced to $36.39/L. According to this analysis, the reutilization of fish waste as liquid fertilizer was not particularly economically attractive at present, and plant-scale production would be necessary for commercialization. This is the first cost-effectiveness analysis of the bioconversion of fish waste into liquid fertilizer.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.37 no.6
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• pp.498-504
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• 2004

Removal of waste generated by Paralichthys olivaceus in the seawater aquarium using a foam separator was investigated. Protein concentration without a foam separator continuously increased until 3 days after stocking and reached at 25 mg/L after 5 days stocking, but protein concentration became lower than the initial protein concentration (2.5 mg/L) with a foam separator. The trends of other fish wastes such as ammonia, total suspended solids (TSS) and chemical oxygen demand (COD) were similar to protein. Dissolved oxygen (DO) in the aquarium decreased below 6.0 mg/L without a foam separator, but with a foam separator the average DO in the aquarium was 7.3 mg/L. Foam separation with the increase of superficial air velocity (SAV) was more effective than that with the fixed SAV. This study showed that wastewater. treatment of seawater aquarium using a foam separator is effective method for a fish waste removal and oxygen supply.

• Journal of Navigation and Port Research
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• v.27 no.3
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• pp.259-266
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• 2003

Due to the growth of population and industrial development at the coastal cities, there has been much increase in necessity to effective control of the wastes into the coastal water and river. The amount of disposal at those waters has been increased rapidly ana it is necessary for us to track of it in order to keep the waterway safe and the water clean. The investigation and research in terms of water quality in these regions have been conducted frequently but the systematic survey of the disposed wastes at the bottom was neglected and/or minor. In this study we surveyed the status of disposed waste distribution at the bottom of coastal water and river from the scanned images. The intensity of sound received by the side scan sonar tow fish from the sea floor provides information as to the general distribution and characteristics of the superficial wastes. The port and starboard side scanned images produced from two arrays of transducers borne on a tow fish connected by tow cable to a tug boat have the area with width of 22m~112m and band of 44m~224m. All data are displayed in real-time on a high-resolution color display ($1280{\times}1024$ pixels) together with position information by DGPS. From the field measurement and analysis of the recorded images, we could draw the location and distribution of bottom disposals. Furthermore, we could make a database system which might be useful for navigation and fundamental for planning the waste reception and process control system.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.467-468
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• 2000

The characterization of mixed microorganisms that have activities of protein- and lipid-degradation were studied to reduce marine pollution by fish wastes and to utilize them again as feeds or fertilizers. There was no mutual inhibition among the microorganisms. The pH and temperature for the mixed culture were found to be 7 and $32^{\circ}C$, respectively. The fish waste were started to degrade after one day, and were almost degraded after 4 days without any odor.

• Korean journal of food and cookery science
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• v.19 no.3
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• pp.390-395
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• 2003

The purposes of this study were to identify the attributes of food and service quality, to examine the levels of satisfaction and plate wastes leftover in school meals and to determine the relationship between student satisfaction and plate wastes. A questionnaire was administrated to 1000 elementary students. The respondents comprised of 505 boys and 494 girls. The satisfaction score with the food service quality was rated as 'average', with a score of 3.06 out of 5. The quality attributes of the food service were categorized into three aspects, which included food, service and hygiene. The satisfaction scores for the food- and hygiene-related factors were 3.20 and 3.24, respectively. However, the aspect of service was rated at 2.74, indicating a low level of satisfaction. The intake rates of soup, kimchi, fish and vegetables were lower than those of other types of foods. Two groups, a no-leftovers group and a leftovers group, were classified based on whether students ate all or left foods. The satisfaction scores for the school food service attributes between the two groups revealed that the no-leftovers group was more highly satisfied with the food service quality than the leftovers group (p＜0.001).

• The Journal of Fisheries Business Administration
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• v.34 no.1
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• pp.115-136
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• 2003

As of late, people who enjoy fishing as a pastime in Korea has dramatically increased on the back of the increased national income and 5-day working system being in place. In the past, people used to fish in inland waters, but fishing on the sea has also shown a steady increase. The expanded fishing community along with increasing types of fishing, however, led to destruction of ecosystem by littering the waters of both inland and sea with wastes and reckless exploitation of fish resources. Fishing activities are usually taking place in inland waters, or on the sea, which is, by definition, a common property, and side effects such as free riding and diseconomy are frequently observed occurring. In other words, everyone can use the property free of charge(except paying fishing lot), and littering the waters with pollutants and wastes stemming from fishing activities is currently rampant. In this context, this paper studies the necessity of introducing fishing license system and its feasibility in Korea from a perspective to improve the national welfare through policy measures based on 'polluter pays principle' to prevent further environmental pollution in the fishing lot and preserve fishery resources. Study shows that introduction of Recreational fishing license system is urgently needed, but it is concluded that it should take more time to implement the system so as to accomodate the differing interests of all parties involved including recreational fishing industry, while strengthening public relation activities to solicit for the people's understanding of the system to be introduced.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.39 no.spc1
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• pp.189-197
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• 2006

To elucidate the organic matter sources in soft-bottom macrozoobenthos at Tongyeong, Korea, macrozoobenthos and their potential food sources were sampled in May 2004, and their C and N stable isotope compositions were analyzed. In total we collected 128 macrozoobenthic species, which ranged from 465 to 3,775 individuals/$m^2$(average 2,108 ind.$m^2$) in density and from 47.8 to 539.9 gwwt/$m^2$(average 366.0 gwwt/$m^2$) in biomass. Cluster and multi-dimensional scaling analyses indicated that the macrozoobenthic community was divided into two distinct groups. Coastal inner stations, where commercial fish cages were established, were dominated by Amphioplus ancistrotus, Scoletoma longifoila and Tharyx sp., whereas open sea stations were dominated by Chaetozone spinosa, Scoletoma longifolia and Capitella capitata. ${\delta}^{13}C$ values of sedimentary organic matter showed a distinct gradient in the range of -18.4 to $-15.2\%_{\circ}$, with a declining trend from the coastal inner stations to open sea stations. This probably reflects the settling rate of organic wastes such as feces and pellets from fish cages near the coastal inner stations. The macrozoobenthos showed a broad ${\delta}^{13}C$ range from -19.5 to $-8.9\%_{\circ}$ at the coastal inner stations, and a relatively narrow range from -21.3 to $-12.9\%_{\circ}$ at the open sea stations. ${\delta}^{13}C$ values of macrozoobenthos paralleled those of sedimentary organic matter, Our isotope results suggested that macrozoobenthos near the coastal inner stations used organic wastes derived from fish cages along with phytoplankton, whereas the macrozoobenthos near the open sea stations used organic wasters derived mainly from phytoplankton.