• 한국발생생물학회지:발생과생식
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• 제19권3호
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• pp.135-144
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• 2015

Heat shock protein (HSP) 70, the highly conserved stress protein families, plays important roles in protecting cells against heat and other stresses in most animal species. In the present study, we identified and characterized four Hsp70 (RuHSP4, RuHSC70, RuHSP12A, RuGRP78) family proteins based on the expressed sequence tag (EST) analysis of the Korean rose bitterling R. uyekii cDNA library. The deduced RuHSP70 family has high amino acid identities of 72-99% with those of other species. Phylogenetic analysis revealed that RuHsp70 family clustered with fish groups (HSP4, HSC70, HSP12A, GRP78) proteins. Quantitative RT-PCR analysis showed the specific expression patterns of RuHsp70 family members in the early developmental stages and several tissues in Korean rose bitterling. The expression of 4 groups of Hsp70 family was detected in all tested tissue. Particularly, Hsp70 family of Korean rose bitterling is highly expressed in hepatopancreas and sexual gonad (testis and ovary). The expression of Hsp70 family was differentially regulated in accordance with early development stage of Rhodeus uyekii.

• Parasites, Hosts and Diseases
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• 제48권4호
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• pp.339-342
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• 2010

Phongsaly Province, located in the northernmost area of Lao PDR, was previously suggested to be endemic for the liver fluke Opisthorchis viverrini infection. To confirm, or rule out, this suggestion, the Phonxay village in the Khoua District, Phongsaly Province, was selected for a survey. Ten volunteers (8 men and 2 women aged 31-57 years) who consumed raw freshwater fish and had gastrointestinal troubles were treated with a single dose of praziquantel (40 mg/kg) and pyrantel pamoate (10 mglkg) and purged with magnesium sulfate to recover any worm parasites. Eight of the 10 volunteers expelled 1 or more species of trematodes, nematodes, or cestodes (worm positive rate; 80%). The worms were morphologically identified as H. taichui (861 worms from 8 people), H. yokogawai (59 from 6 people), Phaneropsolus bonnei (1 from 1 person), Trichostrongylus sp. (2 from 2 people), Ascaris lumbricoides (2 from 1 person), Enterobius vermicularis (11 from 3 people), and Taenia saginata (1 strobila with scolex from 1 person). The results indicate that the mountainous area of Phongsaly Province, Lao PDR, is not endemic for the liver fluke but endemic for intestinal flukes, in particular, Haplorchis taichuiand H. yokogawai.

• 수산해양기술연구
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• 제51권4호
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• pp.583-591
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• 2015

The coastal stow net (stow net hereafter) in Korea is one of the major fishing methods for yellow croaker (Larimichthys polyactis), ribbon fish (Trichiurus lepturus), and anchovy (Engraulis japonicus). In terms of energy efficiency, the stow net fishery is more competitive than towing fishing gears such as trawl gears. The fishing vessels in stow net fishery have consumed less fossil fuel and also have had less carbon dioxide emission into the atmosphere. however, the stow net fishery is necessary to be regulated due to its increased output of the fleet. Therefore, it is required for fisheries authorities to manage the fishing capacity or fishing power for the assurance of fishery's sustainability. For fisheries management authorities, it is necessary to quantify data related to fishing capacity and fishing power to deploy fishery policy in a sustainable way. In terms of data for decision-making, Data envelopment analysis (DEA) method was conducted to estimate fishing capacity. Fishing power index (FPI) was also applied to calculate relative fishing power to approach the problem in a quantitative way.

• 한국환경보건학회지
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• 제43권2호
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• pp.122-129
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• 2017

Objectives: Most of the $PM_{10}$ in subway stations is spread by the train-induced wind from the tunnels. Therefore, in order to improve air quality in subway stations, it is important to remove the $PM_{10}$ from the tunnels. After the installation of PSD (platform screen doors), the influence of train-induced wind and $PM_{10}$ at the platform has decreased, but is estimated to have increased in subway tunnels. This study was conducted to investigate the control of $PM_{10}$ in subway tunnels by applying a 500-fold diluted solution mixed with a humectant using a natural polymer. Methods: For this purpose, we tested the dust reduction effect in a laboratory and corrosion test and water pollution using fish and aquatic plants for the natural dust collecting agent. In the tunnel of a subway station, we used the natural dust collecting agent over 15 days. The study was carried out on $PM_{10}$ control during operation, which accounts for more than 70% of subway dust. Results: As results, the natural dust collecting agent exhibited an excellent dust control effect, and it was safe for water quality and soil. It showed the effect of controlling $PM_{10}$ in the subway tunnel by 49.5- 64.7% over 15 days. The use of the dust collecting agent for the control of $PM_{10}$ could be confirmed in the subway. Conclusion: It is necessary to clearly explain the major portions of chemical components contained in $PM_{10}$ to figure out the characteristics of $PM_{10}$ and to develop effective reduction measures to decrease the adverse effects of $PM_{10}$ in the subway.

• Asian Pacific Journal of Cancer Prevention
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• 제17권4호
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• pp.2111-2114
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• 2016

Opisthorchiasis is a health problem in Thailand particularly in northeast and north regions where have been reported the highest of cholangiocarcinoma. Active surveillance is required, therefore a cross-sectional surveyed was conducted in Nong Bunnak sub-district of Nakhon Ratchasima province, Thailand. A total of 367 participants were selected by multistage sampling from 5 villages located near natural water resources. Participants completed a predesigned questionnaire containing behavior questions regarding liver fluke disease, covering reliability and validity knowledge (Kuder-Richardon-20) = 0.80, attitude and practice (Cronbach's alpha coefficient) = 0.82 and 0.79, respectively. Descriptive statistics included frequencies, percentages, means, and standard deviations. The majority of the participants were female (58.3%), age group between 21-30 years old (42.5%), with primary school education (59.9%), occupation in agriculture (38.1%), and married (80.9%). They had past histories of raw fish consumption (88.3%), stool examination (1.4%), anti-parasite medication used (4.6%). Heads of villages, village health volunteers, television, and village newstations were the main sources for disease information. Participants had a moderate level of behavior regarding liver fluke disease. The mean scored of knowledge regarding liver fluke life cycle, transmission, severities, treatment, prevention and control was 10.9 (SD=0.5), most of them had a moderate level, 95.1%. The mean score for attitude regarding liver fluke prevention and control was 45.7 (SD=9.7), and for practice was 30.6 (SD=10.5). Participants had a moderate level of attitude and practice, 94.5% and 47.7, respectively. This study indicates that health education is required in this community including stool examination for liver fluke as further active surveillance screening.

• 한국수산과학회지
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• 제49권4호
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• pp.445-453
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• 2016

Seahorse Hippocampus abdominalis a marine teleost fish, has long been used as one of the essential materials in traditional Chinese medicine. However, the uses of seahorse have been limited due to its high cost, despite its beneficial biological activities. Seahorse has not been widely explored for its biofunctional properties and active components. In the present study, the enzymatic hydrolysates of seahorse were prepared by using two digestive enzymes (trypsin and pepsin) and five food grade enzymes (neutrase, protamex, alcalase, kojizyme, and flavourzyme). The enzymatic hydrolysates indicated higher hydrolysis yields than its water extract. Among them, the distilled water-pepsin hydrolysate (DP) which was obtained by distilled water extraction followed by pepsin hydrolysis, showed the highest yield and protein content as well as the highest alkyl radical scavenging activity. Also, it provided protective effects against oxidative stress induced by AAPH in vero cell and zebrafish. Further fractionation based on the molecular weight was carried out to identify it’s active components, and < 5 kDa (less than 5 kDa) molecular weight fraction was confirmed to have the highest antioxidant activity. In conclusion, this study suggests that DP of seahorse has antioxidant properties, and might be a novel and useful material from the marine origin for healthy functional foods and cosmetics.

• 한국수산과학회지
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• 제44권3호
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• pp.183-190
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• 2011

This study was performed to assess the antioxidative properties of lipid from aquacultured mackerel Scomber japonicus fed with a diet fortified with conjugated linoleic acid (CLA) and ascidian Halocynthia roretzi tunic extracts by radical scavenging assay. The fish were separated into squid oil (Control) and 2.5% CLA (CA25) groups during the 8-week feeding period. The reducing power of each sample showed high levels of activity compared with ${\alpha}$-tocopherol and butylated-hydroxyanisol (BHA) at 0.2-1.0 mg/mL of lipid. Inhibition of linoleic acid oxidation in samples from Control and CA25 groups showed similar activity after 2 days of incubation at $40^{\circ}C$. The 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide, and hydroxyl radical scavenging activities of CLA and carotenoid-deposited sample (CA25) were higher than those of the Control group. The results indicated that the lipid extracted from the viscera of mackerel showed slightly higher antioxidant activities than that from the muscle.

• 박물관보존과학
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• 제20권
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• pp.1-12
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• 2018

익산시 웅포면 입점리 1호분 출토 금동제유물 편들은 1986년 2월 마을 뒷산에서 한 고등학생에 의해 발견매장문화재로 신고되었고, 이에 국립문화재연구소에서 긴급발굴조사를 실시하였다. 금동대륜, 금동입식, 금동관모, 금동식리 등은 신고자에 의해 수습되어 있었다. 또한 발굴조사 당시 석실 내부가 심하게 훼손되어 많은 수의 금동제유물 편들은 정확한 출토위치를 알 수 없어, 지금까지도 금동관의 조합 관계가 확인되지 않고 있다. 중요 유물인 금동관모, 금동식리 등은 보존처리 후 편 접합을 통해 외형적 모습이 복원되었으며, 금속공예기법으로 새긴 봉황문, 물고기 비늘문, 연화문 등의 문양을 관찰할 수 있었다. 본 글에서는 여러 편들로 파손되어 있는 금동제유물 편들에 대한 특징적 요소와 제작기법을 소개하여 복원의 기초적 학술자료로 제공하고, 이 자료를 바탕으로 입점리 금동관의 원형이 복원될 수 있기를 기대한다.

• 수산해양기술연구
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• 제52권3호
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• pp.276-280
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• 2016

This study investigated luring distributions by water layer of common squid which were targeted by angling fishing vessels equipped with LED and metal-halide lamps using a scientific echosounder with a 120 kHz frequency in order to develop energy-effective underwater fish aggregation devices. In the analysis, angles of a transducer were changed from $0^{\circ}$ to $45^{\circ}$ and were rotated every $10^{\circ}$ horizontally. It was shown that common squid were densely distributed from the surface to 40 m and they were also distributed in directions of $10^{\circ}{\sim}+30^{\circ}$, $-30^{\circ}{\sim}-60^{\circ}$, and $-120^{\circ}{\sim}-130^{\circ}$with the head of vessel as the center. Comparative results of angles of transducer on acoustical densities of common squid distributing in 21~40 m water depth showed an average $101.8m^2/nm^2$ in vertical direction of $0^{\circ}$, $12.3m^2/nm^2$ in angle of $30^{\circ}$, and $42.4m^2/nm^2$ in angle of $45^{\circ}$, respectively. It implied that more considerations on acoustic scattering strength by incidence angle direction of the transducer and swimming oriental angle direction of common squid would be required.

• 대한지역사회영양학회지
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• 제20권4호
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• pp.251-258
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• 2015

Objectives: The purpose of this study was to investigate how to manage food allergy of pre-school children, focusing on the current status of the food allergy in childcare facilities in Hanam which have less than 100 children. Methods: Targeting 159 preschool childcare facilities, survey was carried out for a month in March, 2015. Recovery rate was 66.7%. 106 surveys out of 159 were available for analysis using SPSS statistical program version 19.0. Results: Among 106 facilities, 58 (54.7%) reported that none of their children had a food allergy and 48 (45.3%) reported one more children had a food allergy. Total number of children having a food allergy was 71. Among them, the occurrences of food allergy in males were significantly more than that of the females (p<0.001). Further, children under 2 years of age had significantly more food allergy than the other ages (p < 0.001). The allergic inducing foods were nuts (23.3%), egg (17.8%), milk and dairy products (16.4%), fish and shellfish (13.7%), instant foods (12.3%), fruits (8.2%), soybean (4.1%), meat (2.7%), and cereals (1.4%) in order, and 6 children out of 71 were allergic to more than 2 food items. The clinical symptoms of the food allergy were a skin reaction (87.9%) and an oropharyngeal & respiratory reaction (12.1%). Majority of childcare facilities (80.3%) didn't serve alternative foods for children with food allergy. Necessity for food allergy education was significantly higher in facilities with food allergy issues than without such issues. Conclusions: The Center for Children's Foodservice Management need to educate workers of childcare facilities and parents about managing food allergy and enforce a plan to provide alternative menu to children with food allergies.