• 생명과학회지
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• 제19권2호
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• pp.256-263
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• 2009

쏘가리 젖산탈수소효소(EC 1.1.1.27, lactate dehydrogenase, LDH) $A_4$ 동위효소를 affinity chromatography 및 ultrafiltration으로 정제하였다. 정제된 LDH $A_4$ 동위효소의 분자량은 140.4 kDa이었고 등전점(pI)은 7.0이었다. 효소반응의 최적 pH는 7.5로 나타났다. 피루브산을 기질로 하였을 때 LDH $A_4$ 동위효소의 ${K_m}^{PYR}$값은 $4.86{\times}10^{-5}$ M, $V_{max}$값은 13.31mM/min로 나타났다. LDH $A_4$ 동위효소의 역학실험은 쏘가리가 온수성어류라는 점을 보여주었고 정제된 LDH $A_4$ 동위 효소에 대한 항체는 변온 척추동물의 대사생리학적 특성을 연구하거나 여러 가지 병증의 진단에 유용하게 사용될 수 있을 것이다.

• Fisheries and Aquatic Sciences
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• 제20권4호
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• pp.6.1-6.8
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• 2017

The objective of this research was to study the effect of astaxanthin (AST) on growth performance and antioxidant capacity in golden pompano (Trachinotus ovatus) both in vivo and in vitro. In the in vivo study, two diets were formulated with or without astaxanthin supplementation (D1 and D2; 0 and 200 mg/kg) to feed fish for 6 weeks. In the in vitro study, cells from hepatopancreas of golden pompano were isolated and four treatments with or without astaxanthin and $H_2O_2$ supplementation were applied (control group: without both astaxanthin and $H_2O_2$ treated; $H_2O_2$ group: just with $H_2O_2$ treated; $H_2O_2$ + AST group: with both astaxanthin and $H_2O_2$treated; AST group: just with AST treated). Results of the in vivo study showed that weight gain (WG) and special growth rate (SGR) significantly increased with astaxanthin supplemented (P < 0.05). Feed conversion ratio (FCR) of fish fed D2 diet was significantly lower than that of fish fed D1 diet (P < 0.05). Hepatic total antioxidant capacity (T-AOC) and the reduced glutathione (GSH) of golden pompano fed D2 diet were significant higher than those of fish fed D1 diet (P < 0.05). Superoxide dismutase (SOD) was significantly declined as astaxanthin was supplemented (P < 0.05). Results of the in vitro study showed that the cell viability of $H_2O_2$ group was 52.37% compared to the control group, and it was significantly elevated to 84.18% by astaxanthin supplementation ($H_2O_2$ + AST group) (P < 0.05). The total antioxidant capacity (T-AOC) and the reduced glutathione (GSH) of cell were significant decreased by oxidative stress from $H_2O_2$ (P < 0.05), but it could be raised by astaxanthin supplementation ($H_2O_2$ vs $H_2O_2$ + AST), and the malondialdehyde (MDA) was significant higher in $H_2O_2$ group (P < 0.05) and astaxanthin supplementation could alleviate the cells from lipid peroxidation injury. In conclusion, dietary astaxanthin supplementation can improve the growth performance of golden pompano. Moreover, astaxanthin can improve the golden pompano hepatic antioxidant capacity both in vivo and in vitro study by eliminating the reactive oxygen species.

• 생태와환경
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• 제40권4호
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• pp.495-502
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• 2007

Species biotic index (SBI), based on a fish assemblage, was applied to a stream assessment using long-term ecological fish data set (1996 to 2001) in Boryong dam area, which is located in the mainstream of Ungchun Stream of Chungnam province, Korea. According to the methods of Hilsenhoff (1988), the scores of tolerance guild assigned 10 classes to each species by its habitat and feeding guild, but modified current 7 criteria to 5 scoring standards due to unclear borderline among species. Relative abundance in the species number of upper stream guilds was only 7% of the total, whereas the abundance in the species number of middle to downstream upper stream guilds was 64%. Mean SBI, based on dataset in Site 1 during 1995-2001 averaged 5.10, which was judged as a "good" condition by the rank criteria of SBI. Before the dam construction, mean SBI in the Site 1 was 4.61, indicating a "good" condition, but after the dam construction, mean SBI was 5.60, indicating a "fair" condition. Trajectory analysis in the Site 1 showed significantly (One-way ANOVA, $F_{6,21}=3.26$, p=0.02) different among years, reflecting the changes of fish composition and population density by the dam construction, whereas Site 2 showed no significant changes ($F_{6,21}=1.00$, P =0.45) difference among years. Mean SBI prior to the dam construction in the Site 3 was 4.52 but after the construction, the value was 6.30, indicating a distinct difference between the pre- and post-dam construction. Trajectory analysis at the Site 3 supported this fact: Values of SBI showed significantly ($F_{6,21}$=14.37, p<0.01) different. Mean SBI was 4.67 in the Site 4, indicating a "good" condition in the health and the health rank was same as the sampling sites 1, 2, and 4. Trajectory in the Site 4 showed no significant ($F_{6,21}=2.35$, p=0.07) difference among the years. Overall, our trajectory analysis indicated that three of four sampling sites (sites 1, 3, 4) showed significant decreases (n=7, p<0.05) and that the proportions of sensitive species declined evidently in the sites 1 and 2 and the tolerant species increased in the dam sites. Our outcomes may be used as a key data for diagnosis of the long-term ecological impact in the future in the watershed.

• 한국어류학회지
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• 제10권1호
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• pp.77-86
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• 1998

낙동강 중류의 지류인 위천에서 어류상과 서식상황을 1995년 8월에서 1997년 10월까지 약 2년에 걸쳐 조사한 결과를 요약하면 다음과 같다. 본 조사의 결과, 모두 10과 30속 40종의 어류가 채집되었다. 그 중 잉어과 어류가 26종(65.0%)으로 가장 많았고, 다음은 기름종개과 어류가 5종(12.5%), 동자개과 어류가 2종(5.0%)이었고 나머지는 1종씩으로 이루어져 있었다. 한반도 고유종은 Rhodeus uyekii를 비롯한 16종으로 전체 어종수의 40%를 차지하여 매우 높은 고유성을 나타내었다. 하천 전체로 보면 Zacco temmincki(15.46%), Zacco platypus(14.29%), Squalidus gracilis majimae(11.25%)의 3종이 우점종이었으며, Cyprinzιs carpio, Rhodeus ocellatus, Culter brevicauda, Misgurnus mizolepis, Leiocassis ussuriensis, Silurus asotus, Macropodus chinensis, Channaargus의 8종은 상대 풍부도가 0.1% 미만인 희소종이었다. 지역별 종조성을 보면 상류에서는 Moroco oxycephalus, 중상류에서는 Zacco temmincki, Squalidus gracilis majimae 및 Niwaella multifasciata, 중하류에서는 납자루류와 Zacco temmincki, 하류에서는 Zacco platypus와 Microphysogobio속 어류가 우세하게 나타났다. 군집구조의 분석은 본류의 중류인 St. 9, 10, 11과 쌍계천의 중류인 St. 17, 18, 19의 6지점이 가장 안정되고 다양한 군집의 구조를 지니고 있으며, 상류인 St. 1과 4는 소수의 어종이 지배하고 있는 다양성이 낮은 군집구조를 지니고 있음을 나타내고 있다.

• 한국환경복원기술학회지
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• 제18권2호
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• pp.1-17
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• 2015

Ecological reservoir is a multifunctional space where provides the functions of retention, animal habitat and improvement of ecosystem health and landscape. The ecological reservoir of Anteo Eco Park located in Gwangmyeong-si has established to functions for water purification, maintenance of healthy aquatic ecosystem. Because the Anteo Eco Park is located in the site where nonpoint pollutant materials flow in, Anteo Eco Park has potential factors which aquatic ecosystem health deteriorates and damages the habitat of golden frog(Rana plancyi chosenica) which is restoration target species. Therefore, the purpose of this study is to suggest the plan to manage the variables which impede the right functions of aquatic ecosystem by understanding the causal loop diagram for the change of water quality environment and the interaction of predator-prey through system thinking. The results are as follows. First, the study showed that the individual number of golden frog which is an indicator species of Anteo Eco Park is threatened by snakeheaded fish, which is an upper predator. Therefore, balanced food chain should be hold to protect golden frog by capturing the snakeheaded fish which is individual number's density is high, and the monitoring management of the individual number for predator(snakeheaded fish)-prey(golden frog) should be performed. Second, the study represented that water pollution and carnification is caused by the sediment as the dead body of the large emergent vegetation in the winter cumulates as sediment. Ecological reservoir in Anteo Eco Park has been managed by eliminating the dead body of the large emergent vegetation, but the guideline for the proper density maintenance of vegetation community is additionally needed. Lastly, the study showed that aquatic ecosystem of Anteo Eco Park where is contaminated from the inflow of nonpoint pollutants affects the individual number's decline of golden frog and snakeheaded fish. Accordingly, the creation of a buffer area and a substitution wetland is needed in the periphery of the Anteo Eco Park to control the inflow of nonpoint pollutants including organic matters, nutrients and heavy metals. This study will be helpful that Anteo Eco Park improves the regional landscape and maintain healthy aquatic ecosystem space for the park visitors including local residents.

• 수산해양기술연구
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• 제49권3호
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• pp.301-310
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• 2013

To suggest a standard concerning with the arrangement of bridge equipment, the authors conducted the video observations with 3CCD (charge coupled device) cameras installed on the ceil of the bridge for monitoring the working activities of two bridge teams (the skipper/mate1 and the skipper/mate2) in a Korean coastal large trawler(gross tonnage: 139) for five days from July 30th. 2010 and analyzed of the data. Work elements coded by the work activities were input on the sheet of work analysis by the time unit of 1 sec according to the time occurred. A single work element among the work activities for every 5 minutes was denoted as the number of occurrence. The frequency of equipment usage was limited only in the usage of the equipment. In the case of the navigation and the towing net two ranks were integrated and analyzed. On the other hand, in the case of the casting net and the hauling net, two processes were integrated to as one and then analyzed separately as two ranks. As the results, 15 elements of work was carried out between two bridge teams for the observation; lookout, radar, GPS plotter, fish finder, net monitor, fishing deck, RPM indicator, rudder angle indicator, compass card, for maneuver; steering, ship speed control, trawl winch operation and external communications, paper works and others. It was found that the work load of the skipper per 5 minutes accordance with the navigation, the casting net, the towing net and the hauling net are 20.5 times, 11.9 times, 38.0 times and 9.5 times respectively, the mates are 65.2 times, 66.5 times, 85.7 times and 59.1 times respectively. The radar was shown the highest frequency of the equipment usage and the next was the fish finder, the GPS plotter and the external communications in the case of the navigation. In the case of the towing net the frequency of usage was high the ranking as the radar, the net monitor, the fish finder, the GPS plotter, the steering system and the external communications. In the case of the integrated process both of the casting and hauling net the trawl winch was shown the highest frequency to the skipper and the next was the GPS plotter and the radar, and the steering system was shown the highest frequency to the mate and the next was the radar, the ship speed control system, the GPS plotter, the net monitor and the fish finder.

• The Korean Journal of Physiology and Pharmacology
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• 제4권2호
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• pp.91-97
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• 2000

The purpose of the present study was to determine the preventive effects of combined interventional trial of fish oil treatment and exercise training on insulin resistance of skeletal muscle in high-fat fed rats. Male Wistar rats were randomly divided into chow diet (CD), high-fat diet (HF), high-fat diet with fish oil (FO), high-fat diet with exercise training (EX), and FO+EX groups. The rats in control group were fed chow diet containing, as percents of calories, 58.9% carbohydrate, 12.4% fat, and 28.7% protein. High-fat diet provided 32% energy as lard, 18% as corn oil, 27% as carbohydrate and 23% as casein. The fish oil diet had the same composition as the high fat diet except that 100 g menhaden oil was substituted for corn oil. Insulin sensitivity was assessed by in vitro glucose transport in the soleus muscle after diet treatment and treadmill running for 4 weeks. While the FO or EX only partially prevented insulin resistance on glucose transport and visceral obesity induced by high-fat diet, these interventions completely corrected hyperinsulinemia and hyperglycemia from the high-fat diet. The rats in the FO+EX showed normalized insulin action on glucose transport, plasma chemicals and visceral fat mass. Insulin-mediated glucose transport was negatively associated with total visceral fat mass (r=－0.734; p＜0.000), plasma triglyceride (r=－0.403; p＜0.05) and lepin (r=－0.583; p＜0.001) concentrations with significance. Multiple stepwise regression analysis showed that only total visceral fat mass was independently associated with insulin-mediated glucose transport (r=－0.668; p＜0.000). In conclusion, combined interventional trial of FO+EX recovered insulin resistance on glucose transport of skeletal muscle induced by high-fat diet. Visceral fat mass might be more important factor than plasma TG and leptin to induce insulin resistance on glucose transport of skeletal muscle in high-fat fed rats.

• Journal of Advanced Marine Engineering and Technology
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• 제39권4호
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• pp.495-500
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• 2015

우리나라 주요 양식어종은 넙치, 조피볼락, 참돔이 전체 양식어종의 80% 이상을 차지하고 있다. 본 연구에서는 양식 어종의 다양화와 그에 따른 새로운 양식기법 도입을 위하여 LED파장을 이용하여 강도다리의 성장률을 계측하여 성장에 최적한 LED 파장을 규명하였다. 본 연구에 사용된 LED 파장의 조건은 Red 645nm, Green 525nm, Blue 465nm, 3차 혼합색인 White LED를 사용하였고, 전압의 차이에 따라 각 파장별 Package 배열에 차이를 두었다. 또한 연구에 사용된 조명은 적분구 측정을 통해 동일한 양의 방사속을 조사하도록 조절하여 진행하였으며 실험에 사용된 자어의 평균 체중은 $17.1{\pm}3.3g$, 체장은 $101.5{\pm}12.6mm$이였다. 자어는 사각형($60{\times}45{\times}45cm$) 순환 여과식 수조에 각각 10미씩 수용하여 12주간 사육하였고 매 1주에 체중 및 체장을 계측하여 성장률을 조사하였다. 연구 결과 Green 실험구에서 가장 높은 체중 증가율이 54.03%로 가장 높았으며 다음으로 White 실험구에서 38.47%, Red 실험구에서 36.98% Blue 실험구에서 34.72%로 나타났으며, 체장의 증가도 또한 Green 실험구에서 14.36% 가장 높았으며, White, Red, Blue 순서로 체장의 증가율이 나타났다. 본 연구결과로 강도다리는 사육환경에서 조명에 대한 영향을 크게 받는 것을 알 수 있으며, 나아가서 양식현장에의 적용 가능한 양식용 특수조명 개발과 공학을 기반으로 한 새로운 수산-LED 융합기술의 발전방향을 선도하는 중요한 기초연구가 될 것으로 판단된다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권2호
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• pp.297-305
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• 2003

The family of lemnacae colloquially known as duckweed contains the world' smallest species of flowering plants (macrophytes). Aquatic and free-floating, their most striking qualities are a capacity for explosive reproduction and an almost complete lack of fibrous material. They are widely used for reducing chemical loading in facultative sewage lagoons, but their greatest potential lies in their ability to produce large quantities of protein rich biomass, suitable for feeding to a wide range of animals, including fish, poultry and cattle. Despite these qualities there are numerous impediments to these plants being incorporated into western farming systems. Large genetically determined variations in growth in response to nutrients and climate, apparent anti-nutritional factors, concerns about sequestration of heavy metals and possible transference of pathogens raise questions about the safety and usefulness of these plants. A clear understanding of how to address and overcome these impediments needs to be developed before duckweed is widely accepted for nutrient reclamation and as a source of animal feed.

• 동아시아식생활학회지
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• 제7권4호
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• pp.475-483
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• 1997

The main purpose of this research is to provide Information regarding the preference and intake of calcium of middle school students in the Daegu area. The results were summarized as follows: The recognition score of calcium of the subjects was male 12.85 and female 12.45, respectively. Also the preference scores of calcium source foods of the subjects were male 3.60 and female 3.49. They preferred ice cream, laver, yoghurt and steamed fish cakes in that order, but they disliked cheese and loach soup. The daily calcium intake was 483.78mg(53.8% of RDA) for males and 545.91mg (68.2% of RDA) for females.