• Molecules and Cells
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• v.23 no.3
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• pp.287-303
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• 2007

Expressed sequence tags (ESTs) and differentially expressed cDNAs from the self-fertilizing fish, Kryptolebias marmoratus were mined to develop alternative biomarkers for endocrine-disrupting chemicals (EDCs). 1,577 K. marmoratus cDNA clones were randomly sequenced from the 5'-end. These clones corresponded to 1,518 and 1,519 genes in medaka dbEST and zebrafish dbEST, respectively. Of the matched genes, 197 and 115 genes obtained Unigene IDs in medaka dbEST and zebrafish dbEST, respectively. Many of the annotated genes are potential biomarkers for environmental stresses. In a differential display reverse transcriptase-polymerase chain reaction (DD RT-PCR) study, 56 differential expressed genes were obtained from fish liver exposed to bisphenol A. Of these, 16 genes were identified after BLAST search to GenBank, and the annotated genes were mainly involved in catalytic activity and binding. The expression patterns of these 16 genes were validated by real-time RT-PCR of liver tissue from fish exposed to bisphenol A. Our findings suggest that expression of these 16 genes is modulated by endocrine disrupting chemicals, and therefore that they are potential biomarkers for environmental stress including EDCs exposure.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.7
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• pp.1035-1040
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• 2003

This experiment was conducted to investigate the effects of fat sources on growth performance, nutrient digestibility, serum traits and intestinal morphology in weaning pigs. A total of 128 weaning pigs (Landrace${\times}$Large White${\times}$Duroc, $21{\pm}2$ days of age, $5.82{\pm}0.13kg$ of average initial body weight) were allotted in a randomized complete block (RCB) design with four treatments: 1) corn oil, 2) soybean oil, 3) tallow and 4) fish oil. Each treatment had 8 replicates with 4 pigs per pen. During phase I period (d 0 to 14), pigs fed corn oil or soybean oil diet tended to show higher ADG and FCR than any other treatments although there was no significant difference. During phase II period (d 15 to 28), pigs fed corn oil diet showed better ADG and ADFI than pigs fed soybean oil, tallow or fish oil. For overall period, growth performance of weaning pigs was improved (p<0.05) when pigs were fed soybean oil or corn oil. Apparent digestibility of energy and fat was improved when pigs were fed corn oil diet (p<0.05). Supplementation of corn oil resulted in higher serum triglyceride concentration than the other treatments (p<0.05). However, there was a lower cholesterol concentration when corn oil was provided compared to tallow or fish oil. Pigs fed corn oil tended to have increased villus height compared with soybean oil, tallow or fish oil treatment (p<0.05). This experiment suggested that vegetable oils such as corn oil or soybean oil, were much better fat source for improving growth performance of weaning pigs.

• Clinical and Experimental Pediatrics
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• v.51 no.4
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• pp.426-430
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• 2008

We report clinical, cytogenetic, and fluorescence in situ hybridization (FISH) studies of a patient with ring chromosome 9. She presented with failure to thrive, facial dysmorphysm and mild psychomotor development delay in the absence of major malformations. Peripheral blood karyotype of the patient was 46,XX,r(9)(p24q34). G-band analysis suggested no loss of material in the ring chromosomes. FISH analysis using the subtelomere-specific sequences on chromosome 9p and 9q, revealed 46,XX,r(9)(p24q34),ish r(9)(D9S913-,D9S325+). Failure to detect any hybridization of a probe for the subtelomeric sequences in the ring 9p terminal suggested that this ring arose from breakage in the distal short arm. The cytogenetic and FISH data in our case provided further evidence for the existence of a "complete ring" phenotype with incomplete subtelomeric sequences.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.3
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• pp.309-312
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• 2004

We performed exon trapping in order to locate functional genes on chicken chromosomes (GGA) and to identify functional gene sequences from chicken cosmids. Sequence analysis of 100 clones revealed 17 putative exons, five of which were identified with known sequences in a gene database search: thymopoietin beta (TMPO), U5 snRNP-specific 40 kDa protein (HPRP8BP), dihydropyridine receptor alpha 1 subunit (CACNL1A3), cystein string protein (CPS) and C15orf4. We attempted to map the genes to chicken chromosomes by using FISH and linkage analysis. The chromosomal localizations were GGA1 (TMPO), GGA10 (C15orf4), GGA23 (HPRP8BP) and GGA28 (CPS) by FISH and linkage analysis, while that of CACNL1A3 was predicted to be on a microchromosome by FISH but not by linkage analysis. Comparative mapping analyses between chickens and humans for the genes revealed both known and new synteny. The syntenic conservation between GGA1 and human chromosome (HSA) 12q23 (TMPO) and between GGA10 and HSA15q25 (C15orf4), were consistent with a recent publication, while two new syntenies were observed between GGA28 and HSA20q13.3 in CPS and between GGA23 and HSA1p34-35 in HPRP8BP. The information of presently mapped genes can contribute as anchor markers based on functional genes and the construction of a comparative map.

• Korean journal of food and cookery science
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• v.28 no.6
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• pp.829-837
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• 2012

This study was investigated seafoods provided by school food service and students' preferences for and perceptions of seafoods. The subjects were 275 second grade(age 14-16) students of 4 middle schools in Gyeongnam. The results were as follows. The most main seafoods intake place was 'home'(65.8%). 'School food service' took meaningful ratio(20.7%) of students' seafoods intakes. In the intake amount of seafoods provided by school food service, 'all' took 22.5%(male 31.6%, female 14.1%), 'more than provided' took 1.5%(male 3.0%, female 0%). Male students ate seafoods more than female students did(p<.001). In seafoods providing frequency, '2~3 times a week' took 74.5%, '4~5 times a week' took higher ratio in males' schools, while '0~1 times a week' took higher ratio in females'(p<.05). In perceptions of seafoods, most subjects had positive perceptions as 'good for health'(3.95), 'various kinds'(3.75) except 'good peculiar smell' got smallest point(2.85). In means of learning about seafoods names, 'by looking at everyday menu' took 64.6%. In taking nutrition education, 'no nutrition education' took 69.5%. In preferences for seafoods using 5-point scale, males' preferences were higher than females'(p<.001). 48.1% of males got higher than 4 point, while 14.1% of females did. In improvement measures of seafoods, 'provide various kinds'(47.3%) took highest ratio. In preferences for seafoods by seafoods kinds, preference for 'crustacean' was highest while preferences for 'shell fish' and 'fish' were relatively low. Both male and female students highly preferred laver, shrimp, swimming crab, small octopus, fish cake and tuna canned goods. Male students' preferences were higher than female students' for most kinds of seafoods. In preferences for seafoods by cooking methods, preferences for 'grilled', 'stir fried', 'pan fried' were relatively high, 'braised', 'deep fried', 'steamed' were relatively low. Males' preferences were higher than females' for every cooking method except 'steamed'.

• The Journal of Fisheries Business Administration
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• v.50 no.3
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• pp.1-15
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• 2019

The purpose of this study is to analyze the differences by comparing consumption tendency of the Flatfish and Salmon using focus group interview (FGI). The FGI was conducted on university students in their 20s, with a total of 38 respondents surveyed in seven focus groups. Flatfish was recognized as a food in the form of popular sliced raw fish and had customer image that fit for middle-aged men in their 40s and 50s while eating with alcohol. 20s were perceived to be out of date with their age group as flatfish was considered old fish that did not match their age. In addition, they felt low accessibility to flatfish. Salmon was perceived by 20s as a food with a higher appeal than flatfish. Salmon has been creating a brand identity in their 20s and 30s for fashionable and trendy food that appealed to younger generations. They considered characteristics of salmon high in intangible value, such as something sophisticated, trendy, and beautiful. 20s consumed various forms of salmon including salmon sushi, salmon bowl, and salmon fillet, and they perceived it as a psychologically close food that can be easily accessible. In this study, the color of species (orange color of salmon), accessibility, restaurant image, and social media exposure were found to influence the consumption patterns of two species.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.55 no.3
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• pp.181-189
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• 2019

Discarding juvenile fishes under girth 16 cm nearly equal to inner perimeter of codend mesh size collected by a cover net method in bottom trawl. The body size of the main five species (mackerel, horse mackerel, sea bream, melon seed and black throat seaperch) was measured for their body length, girth, weight, height and width and analyzed size selectivity. Frequency of penetrating fish as retention in a cover net was less than 40% of total number of juvenile discarding fish. The most of body length or girth of five species were significantly different between in the codend and in the cover net. The 50% selection girth in the cover net ranged 8-11 cm were smaller than those in the codend ranged 9-13 cm by the species respectively. The 50% selection body length was significantly related with the ratio of body height (H) by body width (W) both for in the codend or in the cover net while 50% selection girth was not significantly related with H/W. Furthermore 50% selection fish size by fish species between in the codend and in the cover net was not significantly different both in body length or girth. Therefore, the girth selectivity represented possibly as one unique value regarding fish body shape was considered as more useful method for multi-species catch in trawl.

• International Journal of Advanced Culture Technology
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• v.11 no.2
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• pp.375-380
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• 2023

The study was conducted in Manu-stream, located in Paju, Gyeonggi-do, from January 2021 to December 2021. The survey points were selected in the upper and lower streams based on where thermal discharged to investigate water temperature and fish species and biological community analysis and growth rate were analyzed. The average annual water temperature difference between the upper and lower stream is about 11.0℃, and in the case of the lower stream area, the water temperature is maintained at 20.0℃ or more per year. Fish that appeared during the survey period decreased lower stream compared to the upper stream, which is believed to be the result of a decrease in temperature-sensitive species as the simple riverbed structure and water temperature increased compared to the upper stream. As a result of biological community analysis, it showed a relatively stable community state at the upper stream. The growth rate of fish has a high regression coefficient b value in lower streams throughout the four seasons. It showed relatively good growth lower stream, with a high water temperature. However, the results of each survey point are similar from season to season. The indicator species is a resistant intermediate species, and the range of resistance to water temperature is wide, so it is judged that water temperature's effect on the indicator species' growth is low.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.12
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• pp.7621-7628
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• 2013

Human epidermal growth factor receptor (HER) status is an important prognostic factor in breast cancer. There is no globally accepted method for determining its status, and which method is most precise is still a matter of debate. We here analyzed HER2 mRNA expression by quantitative reverse transcription-PCR (qRT-PCR) and HER2 DNA amplification using multiplex ligation-dependent probe amplification (MLPA). In parallel, we performed a routine evaluation of HER2 protein by immunohistochemistry (IHC). To assess the accuracy of the RT-PCR and MLPA techniques, a combination of IHC and fluorescence in situ hybridization (FISH) was used, substituting FISH when the results of IHC were ambiguous (2+) and for those IHC results that disagreed with MLPA and qRT-PCR, this approach being termed IHC-FISH. The IHC results for four samples were not compatible with the MLPA and qRT-PCR results; the MLPA and qRT-PCR results for these samples were confirmed by FISH. The correlations between IHC-FISH and qRT-PCR or MLPA were 0.945 and 0.973, respectively. The ASCO/CAP guideline IHC/FISH correlation with MLPA was (0.827) and with RT-PCR was (0.854). The correlations between the IHC results (0, 1+ as negative, and 3+ as positive) and qRT-PCR and MLPA techniques were 0.743 and 0.831, respectively. Given the shortcomings of IHC analysis and greater correlations between MLPA, qRT-PCR, and FISH methods than IHC analysis alone with each of these three methods, we propose that MLPA and real-time PCR are good alternatives to IHC. However a suitable cut-off point for qRTPCR is a prerequisite for determining the exact status of HER2.

• Journal of Trauma and Injury
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• v.28 no.3
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• pp.206-210
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• 2015

Cervicofacial subcutaneous emphysema is a rare complication mainly resulting from dental procedures. The majority of cases are self-limiting and benign in clinical nature. However, although entry sites may be quite small and superficial, it can result in potential respiratory difficulties, such as pneumothorax and pneumomediastinum. We present the first case in which facial subcutaneous emphysema developed in a 6-year-old boy following use of a fish cake skewer while eating.