• Journal of Aquaculture
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• v.22 no.1
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• pp.11-15
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• 2009

Effect of fasting and refeeding on growth and blood chemistry of juvenile olive flounder Paralichthys olivaceus L. was investigated when fish achieved compensatory growth. Fish were fed the experimental diet for 6 days a week. Five treatments in triplicate were prepared: C, S1, S2, S3 and S4. Fish in the control group (C) were hand-fed to apparent satiation twice a day. Fish in treatments of S1, S2, S3 and S4 experienced 1, 2, 3 and 4 weeks of starvation and were then hand-fed to satiation twice daily during the remaining 7, 6, 5 and 4 weeks of the experiment, respectively. Weight gain of fish in C, S1 and S2 were higher than those of fish in S3 and S4. A significant difference in plasma total protein, glucose, triglyceride, $T_3$ and $T_4$ was observed in between starved and refed fish for the rest periods of the feeding trial. Plasma total protein and $T_3$ of flounder decreased with week of fasting and following correlationships were obtained; Y (Total protein) = -0.13X (week of fasting) + 1.54, $R^2=0.9792$ and $Y(T_3)=-11.48X$ (week of fasting) + 79.57, $R^2=0.8822$, respectively.

• Journal of fish pathology
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• v.22 no.2
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• pp.173-177
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• 2009

Lymphocystis disease virus (LCDV) was detected from olive flounder Paralichthys olivaceus, painted glass fish Chanda baculis, gourami Trichogaster leeri and rockfish Sebastes schlegeli, and proteins of the viruses were compared. The major capsid protein (MCP) gene-specific primer sets successfully amplified approximately 1300 bp nucleotides from the olive flounder and 600 bp nucleotides from painted glass fish, gourami and rockfish isolates, respectively. In western blotting analysis using anti-LCDV mouse polyclonal serum, major antigenic proteins had 21, 26, 45, 50, 80, 110 and 120 kDa in olive flounder, 26, 47 and 80 kDa in painted glass fish, 26, 46, 80 and 92 kDa in gourami, 26, 44, 49, 80 and 105 in rockfish, respectively. All the marine and freshwater isolates showed only common antigens of approximately 26 kDa and 80 kDa. These results suggest that antigenic protein profiles of LCDVs may vary depending upon fish species.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.73-73
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• 2003

This study was carried out to evaluate the utilization of fermented skipjack tuna viscera (FSTV) in the diet for juvenile abalone Haliotis discus hannai. Lactobacillus bulgaricus was used for fermentation of skipjack tuna viscera. Eight isonitrogenous (about 30% crude protein) diets were formulated to include different levels (0%, 10%, 20% and 30%) of FSTV as a replacer of either dietary fish meal or soybean meal. Three replicate groups of abalone were fed the experimental diets containing different levels of FSTV for 7 weeks. The inclusion of FSTV up to 30% in fish meal-based diet had no significant effect on survival, body weight, shell growth, and proximate composition of abalone (P>0.05). Weight gain of abalone fed the diet substituting 10% FSTV for soybean meal was not significantly different to that of abalone fed the control diet, however this value decreased in abalone fed the 20% and 30% FSTV (P<0.05).The contents of crude protein and lipid of soft body in abalone fed soybean meal-based diets were significantly affected by dietary FSTV level (P<0.05). The results of this study indicate that FSTV can be used as a partial substitute protein source for fish meal or soybean meal in the formulated diet for juvenile abalone.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.49 no.5
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• pp.607-611
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• 2016

This study was conducted to investigate the effects of different water temperatures on growth, survival, biochemical composition, and blood physiological parameters of juvenile mandarin fish Siniperca scherzeri. Feed-trained juvenile fish were subjected to four water temperatures (20, 23, 26 and 29℃) with two replicate groups. The fish were fed to apparent satiation twice daily using a formulated diet containing 55% crude protein and 6% crude lipid. After the 8-week feeding trial, survival was >96% in all groups. Weight gain and feed efficiency of fish reared at 26 and 29℃ were higher than those reared at 20℃. The protein efficiency ratio, daily feed intake, and whole body proximate composition were not affected by water temperature. These results indicate that a suitable water temperature range for optimal growth and feed efficiency of juvenile mandarin fish is 26-29℃ under these experimental conditions.

• Journal of Aquaculture
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• v.18 no.3
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• pp.215-221
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• 2005

This study was conducted to investigate the effects of dietary supplementation of cottonseed and soybean meal on growth performance of juvenile olive flounder (Paralichthys olivaceus). Nine hundred fish $(0.74{\pm}0.11g)$ in the early juvenile stage were randomly divided into 15 groups, and 3 groups were fed one of five isonitrogenous (56% CP) and isocaloric $(16.3\;MJ\;kg^{-1})$ diets replacing 0, 10, 20, 30, and 40% of fish meal protein by equal proportion (1:1, w:w) of cottonseed and soybean meal (CS) (designated by Control, CS10, CS20, CS30, and CS40, respectively). A solvent extracted cottonseed meal containing high crude protein (44%) and low fiber content (<12%) was used in this study. After 10 weeks of feeding trial, the growth offish fed diets CS10, CS20, and CS30 were not significantly (P>0.05) different compared to that of fish fed the control diet. However, diet CS40 exhibited significantly lower (P<0.05) growth performance than the control diet. No differences were observed in whole body composition of fish fed all the experimental diets. This study indicates that mixture of cottonseed and soybean meal with lysine and methionine supplementation can replace up to 30% fish meal protein in diet for olive founder at the early juvenile stage. However, we suggest that 20% of fish meal protein replacement by cottonseed (10%) and soybean (10%) meal can be the optimum level for commercial use in safety according to the growth performances.

• Journal of the Korean Society of Food Science and Nutrition
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• v.30 no.4
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• pp.574-578
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• 2001

Myofibrillar proteins were prepared from red muscle and white muscle of fresh water fish and sea water fish, and their thermostabilities and effect of temperature on the myofibrillar ATPase activities were compared. Differences in temperature dependency of myofibrillar ATPase activities were found between two species. Thermodynamic data for inactivation of myofibrillar proteins, such as D value, Z value, $\Delta$ $H^{{\neq}}$, $\Delta$ $G^{{\neq}}$ and $\Delta$ $S^{\neq}$ revealed that thermostabilities of myofibrillar proteins from fresh water fish were higher than those from sea water fish, and that myofibrillar proteins from red muscle were more heat labile than those from white muscle.

• Korean Journal of Food Science and Technology
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• v.26 no.5
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• pp.479-483
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• 1994

The effects of the different protein source on serum cholesterol levels were studied in SD strain male rats. Fish protein prepared by the method of SUZUKI from Alaska Pollack (Theragra chalcogramma) was compared with casein and soybean protein isolate. Each protein source was incoporated into a cholesterol-free diet in order to provide a protein level of 20% for 2 weeks. The result obtained are as followed: Concentration of total-cholesterol, LDL-cholesterol, total-cholesterol/HDL-cholesterol and triglyceride in rats fed with fish protein group were significantly lower than those of rats fed with casein and similar to those of rats fed with soybean protein. In addition, it was shown that the ratio of Lys/Arg and Gly/Met+Cys of fish protein was close to that of soybean protein.

• Fisheries and Aquatic Sciences
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• v.23 no.1
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• pp.1.1-1.14
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• 2020

Background: Dietary protein requirements are dependent on a variety of factors and water temperature is one of the most important abiotic factors affecting protein requirement of fish. This study was, therefore, conducted to investigate effects of water temperature on dietary protein requirement of fry Heteropneustes fossilis which has high demand in most of the Asian markets. Methods: Quadruplicate groups of 30 fish per treatment (2.97 ± 0.65 cm; 5.11 ± 0.34 g) were fed seven isoenergetic diets (17.9 kJ g^-1 gross energy; 14.99 kJ g^-1 digestible energy) containing dietary protein levels ranging from 28 to 52% at two water temperatures (18 and 26 ℃). Experimental diets were fed to apparent satiation as semi-moist cakes thrice daily at 17:00, 12:00, and 17:30 h for 12 weeks. For precise information, various growth parameters, protein deposition, hematological parameters, metabolic enzymes, and stress response were analyzed, and effects of water temperature on dietary protein requirement was recommended on the basis of response from above parameters. Results: Groups held at 26 ℃ attained best growth, feed conversion, and protein deposition at 44% dietary protein indicating that temperature affected dietary protein requirement for optimum growth of H. fossilis fry and protein requirement seems to be satisfied with 44% dietary protein. Interestingly, interactive effects of both dietary protein levels and temperature were not found (P > 0.05). Fish reared at 18 ℃ had comparatively higher values for aspartate and alanine transferases than those reared at 26 ℃ water temperature which exhibited normal physiological value for these enzymes indicating that body metabolism was normal at this temperature. Hematological parameters also followed same pattern. Furthermore, fish reared at 26 ℃ water temperature exhibited more resistant to thermal stress (P < 0.05). The 95% maximum plateau of protein deposition data using second-degree polynomial regression analyses exhibited dietary protein requirement of fry H. fossilis between 40.8 and 41.8% of diet at 26 ℃ water temperature. The recommended range of dietary protein level and protein/digestible energy ratio for fry H. fossilis is 40.8-41.8% and 27.21-27.88 mg protein kJ^-1 digestible energy, respectively. Conclusions: Information developed is of high significance for optimizing growth potential by making better utilization of nutrient at 26 ℃ and, to develop effective management strategies for mass culture of this highly preferred fish species.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.45 no.2
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• pp.132-138
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• 2012

An 8-week feeding experiment was conducted to evaluate three types of squid Sepia esculenta liver powder (SLP) as a dietary protein source replacing fish meal (FM) in juvenile olive flounder Paralichthys olivaceus. To replace FM, six experimental diets were formulated with three types (A, B, C) of SLP at the 5 and 10%: SLP-A5, SLP-A10, SLP-B5, SLP-B10, SLP-C5, and SLP-C10. One control diet contained 100% FM as the main protein source and another was a commercial diet (Com). Fish with an average body weight of $22.8{\pm}0.4$ g ($mean{\pm}SD$) were allocated randomly to aquaria in groups of 20 fish and fed the experimental diets in triplicate to satiation. The weight gain (WG) and specific growth rate (SGR) of fish fed the SLP-C10 diet were lower than those of fish fed the FM and SLP-B5 diets. No significant difference was observed in the WG and SGR among fish fed the diets other than SLP-C10. The feed efficiency (FE) and protein efficiency ratio (PER) of fish fed each SLP diet did not differ from those fed the control diet. However, fish fed SLP-C5 and SLP-C10 had a lower FE and PER than the fish fed commercial, SLP-A5 and SLP-B5 diets. Each SLP diet except for SLP-C10 could replace up to 10% of FM for juvenile olive flounder. The results of this experiment provide information that will assist in formulating an inexpensive practical diet containing SLP for juvenile olive flounder.

• Environmental Analysis Health and Toxicology
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• v.18 no.2
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• pp.155-163
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• 2003

Fluoranthene, a common polycyclicaromatic hydrocarbon (PAH), exhibits phototoxicity which may affect aquatic organisms. The eventual goal of this study is to develop a biomarker of Japanese medaka (Oryzias latipes) used in monitoring hazardous chemicals in the ecosystem. In this study we investigated neural toxicity of fluoranthene in Japanese medaka (Oryzias latipes) along with comparative analysis of corresponding behavioral response. The untreated individuals shooed normal behavioral characteristics (i. e., smooth and linear movements). The treated fish, however, showed stopping and abrupt change of orientation (100 ppb), and severely reduced locomotive activity and enhanced surfacing activity (1,000 ppb). Treatment of the medaka fish with fluoranthene caused a significant suppresson of acetycholine esterase (AChE) activities in the body portion but not in the head portion. When fish were exposed to 1,000 ppb of fluoranthene for 24 hr, the body AChE activities decreased from 126.${\pm}$31.89 (nmoles substrate hydrolyzed per min per mg protein) to 49.51${\pm}$11.99. Expressions of tyrosine hydroxylase (TH) protein in the different organs from both head and body portions were comparatively analyzed using an immunohistochemical technique. Five organs of the medaka fish showing a strong TH protein expression were the olfactory bulb, hypothalamus, optic lobe, pons and myelencephalon regions. This study provides molecular and neurobehavioral bases of a biomonitoring system for toxic chemicals using fish as a model organism.