• Asian-Australasian Journal of Animal Sciences
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• 제19권7호
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• pp.1033-1039
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• 2006

The metabolic response of Labeo rohita to thermal acclimation was assessed. Advanced fingerlings of L. rohita (average weight $31{\pm}1.4g$) were acclimated to 31, 33 and $36^{\circ}C$ compared with ambient temperatures ($26^{\circ}C$) for 30 days and different enzymes associated with stress response were estimated. Glycolytic enzyme-Lactate dehydrogenase, (LDH, E.C.1.1.1.27), TCA cycle enzyme-Malate dehydrogenase (MDH, E.C.1.1.1.37), Protein metabolizing enzymes-Aspartate amino transferase (AST, E.C.2.6.1.1) and Alanine amino transferase (ALT, E.C.2.6.1.2) of liver, gill and muscle, Gluconeogenic enzymes-Fructose 1,6 Bi phosphatase (FBPase, E.C. 3.1.3.11) and Glucose 6 phosphatase (G6Pase, E.C. 3.1.3.9) of liver and kidney were significantly (p<0.05) different with increasing acclimation temperatures. Heat Shock Protein-70 (HSP-70) was expressed in increasing intensity at 31, 33 and $36^{\circ}C$ but was not expressed at $26^{\circ}C$. Results suggest that higher acclimation temperatures enhance metabolism and L. rohita maintains homeostasis between $26-36^{\circ}C$ via an acclimation episode. Such adaptation appears to be facilitated by resorting to gluconeogenic and glycogenolytic pathways for energy mobilization and induction of HSPs.

• 한국물환경학회지
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• 제32권5호
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• pp.419-432
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• 2016

이 연구의 목적은 응천에서 채집한 담수 붕어(Carassius auratus)의 산화적 스트레스와 조직병리학적 손상을 분석하여 하수처리장 방류수의 영향을 평가하는 것이다. 방류수 혼합 지점과 하류 지점에서 채집한 붕어의 아가미, 간, 콩팥의 과산화수소 분해효소(catalase)의 활동도는 상류 지점과 비교하여 더 높은 것으로 나타났다. 또한, 혼합 지점의 아가미와 간의 글루타치온 S-전이효소(glutathione-S-transferase)의 활동도는 상류 지점과 비교하여 유의하게 높았으며(p < 0.05), 상류 지점보다 유의하게 더 증가된 간의 지질 과산화(lipid peroxidation)가 혼합 지점에서 관찰되었다. 붕어의 조직병리학적 손상의 정도는 간 > 콩팥 > 아가미순으로 나타났다. 이 결과는 간이 방류수의 영향을 가장 크게 받는다는 것을 의미한다. 붕어의 조직 손상도(degree of tissue changes)는 혼합 지점에서 채집한 시료(30.98 ± 5.40)에서 가장 컸으며, 그 다음은 하류 지점 시료(19.28 ± 4.31)이고 가장 낮은 것은 상류 지점 시료(4.83 ± 2.67)로 나타났다. 이러한 결과는 혼합 지점에서 채집한 붕어가 방류수에 가장 큰 영향을 받았다는 것을 의미하며, 산화적 스트레스와 조직병리학적 지표들이 방류수로 오염된 하천을 모니터링하는데 유용하다는 것을 나타낸다.

• 한국수산과학회지
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• 제43권5호
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• pp.445-450
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• 2010

This study investigated the effects of dietary lipid sources on growth performance and body composition of juvenile far eastern catfish, Silurus asotus. Three replicate groups of fish (average weight 3.6 g) were fed with one of the following experimental diets containing 10% beef tallow (BT), 5% BT plus 5% corn oil (CO), 5% BT plus 5% linseed oil (LO), or 5% BT plus 5% squid liver oil (SO) as the lipid source for 5 weeks. No significant difference was observed in the survival among groups. The weight gain of fish fed the LO (high in 18:3n-3) and SO (high in n-3 highly unsaturated fatty acid) diets was significantly higher than that of the fish fed the CO (high in 18:2n-6) and BT diets (P<0.05). The feed efficiency of fish fed LO and SO diets was significantly higher than that of the fish fed the BT diet (P<0.05), but not significantly different from that of the fish fed the CO diet. The protein efficiency ratio of fish fed the SO diet was significantly higher than that of fish fed the CO and BT diets (P<0.05), but not significantly different from that of fish fed the LO diet. The 18:1n-9 of whole-body polar lipid fraction in fish fed the BT diet increased compared to that of fish fed the other diets. Fish fed the CO and LO diets had significantly higher contents of 18:2n-6 and 20:4n-6, and 18:3n-3, than the fish fed the other diets in polar and non-polar lipid fractions, respectively (P<0.05). Significantly higher contents of 20:5n-3 and 22:6n-3 were observed in the whole-body polar lipid fraction of fish fed the SO diet compared with fish fed the other diets (P<0.05). The study results indicate that linseed oil and squid liver oil containing n-3 fatty acids are good dietary lipid sources for the growth of far eastern catfish.

• Fisheries and Aquatic Sciences
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• 제26권10호
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• pp.583-592
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• 2023

Celebes rainbow (Marosantherina ladigesi) is one of Indonesia's exported ornamental fish commodities, but the exploitation of this fish only relies on wild catches. The rise of unlimited fishing, especially those using poison, has changed the aquatic environment, threatening sustainability and causing fish extinction. This study aimed to evaluate the effectiveness of several types of feed in improving the absolute growth rate (AGR), specific growth rate (SGR), survival rate (SR), feed conversion ratio (FCR), feed efficiency (FE), hematology, and immune response of Celebes rainbow. The fish used in this study were male ornamental Celebes rainbow (M. ladigesi) weighing 1.32 ± 0.21 g/ind, reared in 54 L-aquariums at a stocking density of 30 individuals/aquarium for six weeks. The fish were fed according to the test diet consisting of live Tubifex sp worms, dry Tubifex sp worms, Spirulina platensis, and crumble pellets. The parameters observed were AGR, SGR, SR, FCR, FE, hematology, intestinal histology, liver histology, and a challenge test with the pathogenic bacteria Aeromonas hydrophila. The results showed that fish-fed live Tubifex sp worms had better AGR, SGR, SR, FCR, FE, hematology, and disease resistance compared to all other treatments. These results indicate that live Tubifex sp worms are the best feed for rearing Celebes rainbow.

• 한국어병학회지
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• 제23권2호
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• pp.165-176
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• 2010

In May 2007, mass mortality of Israeli carp, Cyprinus carpio L., was occured on a pond farm located in Jeollabukdo Province, Korea. The mortality rates reached up to 2% of the total fish in the farm per day. Typical clinical signs were abdominal distension, reddish foci on the skin, enteritis, liver congestion and enlarged spleen and kidney. On the basis of biochemical characteristics and 16S rDNA sequence, the causative bacteria isolated from affected carp were identified as Aeromonas veronii. Histologically, degeneration of hepatocytes and congestion in sinusoids were observed in the liver. Spleen showed hemorrhage and the destruction of the sheathed tissues. In kidney, necrotized renal tubules and glomerular destructions were observed. Intestinal tissues revealed necrotized and severe hemorrhage. Mass hemorrhage was observed in muscles. This is the first report that A. veronii caused mortality in cultured Israeli carp in Korea.

• Asian-Australasian Journal of Animal Sciences
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• 제24권8호
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• pp.1148-1156
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• 2011

Compensatory growth of juvenile olive flounder Paralichthys olivaceus fed different diets with different feeding regime was compared. Four hundred fifty fish (twenty five fish per tank) were randomly distributed into 18 of 180 L flow-through tanks. Six treatments were prepared: fish were hand-fed with the control (C) diet to satiation twice a day, six days a week, for 8 weeks (C-8W treatment); and other groups of fish were starved for 2 weeks and then fed with the C, high protein (HP), high carbohydrate (HC), high lipid (HL), and combined protein, carbohydrate and lipid (CPCL) diets to satiation twice a day, six days a week, for 6 weeks, referred to as C-6W, HP-6W, HC-6W, HL-6W, and CPCL-6W treatments, respectively. Final body weight of fish in HP-6W treatment was higher than that of fish in C-6W, but not different from that of fish in C-8W, HC-6W, HL-6W and CPCL-6W treatments. Specific growth rate of fish in HP-6W treatment was higher than that of fish in all other treatments except for fish in CPCL-6W treatment. Feeding rate of fish in C-8W treatment was higher than that of fish in HP-6W, HC-6W, HL-6W and CPCL-6W treatments, but not different from that of fish in C-6W treatment. In addition, feeding rate of fish in C-6W treatment was higher than that of fish in HP-6W, HL-6W and CPCL-6W treatments. Feed and protein efficiency ratios of fish in HP-6W, HC-6W, HL-6W and CPCL-6W treatments were higher than those of fish in C-6W treatment. None of moisture, crude protein and ash content of the whole body of fish excluding the liver was different among treatments. Dietary supplementation of protein, carbohydrate, lipid and their combination could improve compensatory growth of fish when fish were fed for 6 weeks after 2-week feed deprivation; especially, supplementation of dietary protein was the most effective to improve compensatory growth of fish.

• 환경생물
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• 제37권4호
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• pp.735-740
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• 2019

해산송사리, Oryzias dancena 2배체와 유도 3배체 각 조직에서의 세포주기를 비교, 분석하였다. G₁, S, G₂+M기의 분포 빈도에서 꼬리지느러미인 경우 2배체에서는 85.8%, 7.6% 및 6.9%이었으며 유도 3배체에서는 91.2%, 3.6% 및 5.2%이었다. 간 조직인 경우 2배체에서는 78.4%, 10.6%, 및 11.0%이었으며 유도 3배체는 86.2%, 5.9% 및 7.9%이었다. 아가미 조직인 경우 2배체에서는 79.3%, 9.4% 및 11.3%이었으며 유도 3배체는 85.7%, 5.4% 및 8.9%이었다. 2배체와 유도 3배체에서 조직 간 세포 주기 빈도에서 유의한 차이가 있었다(p<0.05). Mitosis (체세포분열)은 유도 3배체에 비해 2배체가 더욱 활성이 있었으며, 이러한 체세포분열은 2배체와 유도 3배체 모두에서 꼬리지느러미 조직 보다는 간 조직과 아가미 조직에서 더욱 왕성하였다.

• 한국어병학회지
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• 제32권2호
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• pp.81-89
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• 2019

As an epidemiological survey, mortality of marbled rockfish, Sebastiscus tertius observed from a fish farm in Gyeongnam province of South Korea. The major macroscopic sign of the diseased fish was severe multifocal dermal ulceration. Histological observation revealed inflammation, necrosis and colonization of bacteria in various tissues (gill, liver, spleen and kidney). Bacteria was isolated from spleen and kidney in moribund and mortality fish. Seven bacterial isolates from the diseased fish were identified as Aeromonas salmonicida subsp. salmonicida using API 20E and 20NE, API 50CH API ZYM system. Under light microscopy, infected marbled rockfish showed the lifting of the lamella epidermal layer, edematous changes and hypertrophy of epithelial cell in the gill filament. The atrophy of the mucosal fold, erythema in the intestine, and the necrosis of hematopoietic tissue and renal tubule cells with karyolysis were observed in the kidney. In this study was demonstrated the histological reaction of red marbled rockfish infected by Aeromonas salmonicida. Furthermore, this is the first account of extensive dermatitis in Sebastiscus tertius due to atypical A. salmonicida infection, which has high potential in aquaculture among native fish species.

• Journal of Nutrition and Health
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• 제31권6호
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• pp.1014-1023
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• 1998

The influences of fish oil and different levels of vitamin I supplement on hepatocellular chemical carcinogenesis have been studied. Male Sprague-Dawley rats received diethylnitrosamine (DEN)(200mg/kg body weight) and were subjected to two-thirds partial hepatectomy to induce murine chemical hepatocarcinogenic procedure. Placental glutathione S-transferase(GST-P) positive foci area, antioxidant enzymes(Cu/Zn-superoxide dismutase(SOD), catalase, glutathione reductase (GR), total- glutathione peroxidase (TGPx), glutathione S -transferase (GST)), glucose 6-phosphatase (G6Pase) activities, and lipid peroxidation of microsomes(thiobarbituric acid reactive substances (TBARS)) were measured. Experimental animals were fed 15% corn or fish oil with 0, 40, 1,000, 10,000IU vitamin E /kg diet for 8 weeks. Vitamin E supplements decreased the area of GST-P positive foci in both groups. The higher the vitamin E levels, the smaller the area of GST-P positive foci were noticed. Compared to 0 IU vitamin E, 40 IU in corn oil and 1,000 IU in fish oil groups were effective in decreasing G57-P positive foci area. Fish oil groups tended to have smaller area of GST-P positive foci. fish oil groups showed lower body weight, lower activities of Cu/Zn-SOD and TGPx, higher TBARS contents, higher activities of GST, catalase, G6Pase, GR and higher liver/body ratio than corn oil groups. As the level of vitamin I increased, GST-P positive foci count, catalase activities, and TBARS tended to decrease. G6Pase activities tended to increase in both groups. At higher vitamin E levels, GST activities tended to decrease in fish oil groups. These results suggest that vitamin I has suppressive offects on hepatocellular chemical carcinogenesis probably through antioxidant eH:cts decreasing TBARS contents, $H_2O$$_2$, and organic peroxides. fish oil tended to have greated suppressive offects than corn oil on hepatocellular carcinogenesis. (Korean J Nutrition 31(6) : 1014-1023, 1998)

• Fisheries and Aquatic Sciences
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• 제23권2호
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• pp.4.1-4.9
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• 2020

Growth of fish is controlled by various environmental factors, including water temperature (WT). WT is also a major factor that affects the eating behavior of fish. In this study, we studied the relationship between WT and expression profiles of factors involved in appetite regulation and digestion in red spotted grouper. Fish were divided into three groups with 30 fish per group and reared for 2 weeks in a recirculating system at three different WT (natural WT, 20 ℃, and 25 ℃). After 2 weeks, they were sacrificed immediately after food intake (+ 0 h), 3 h after (+ 3 h), 6 h after (+ 6 h), and 3 h before being fed the next day (- 3 h). Fish samples were obtained for analysis of mRNA expression levels of cholecystokinin (CCK), leptin A (LepA), leptin B (LepB), and neuropeptide Y (NPY) in the brain, liver, and intestine. Expression of CCK and NPY mRNA was significantly higher at 25 ℃ compared to that at natural WT and 20 ℃, but no difference was observed for leptin. This suggests that the red spotted grouper shows characteristics typical of subtropical fish and has higher levels of feeding and digestion when reared at 25 ℃ water temperature condition.