• Mass Spectrometry Letters
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• 제6권4호
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• pp.105-111
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• 2015

Sixteen compounds of Neobalanocarpus heimii were successfully identified directly from their plant extract using a triple quadrupole LC-MS/MS system. In order to fulfil the objectives of this work, a series of stilbene oligomers of various degrees of condensation were isolated and their structure are characterized. Out of these, four are resveratrol dimers, three trimers, and nine tetramers. The isolation process was done on a fully automated semi-preparative HPLC system. Their structures were elucidated on the basis of 1D- and 2D-NMR as well as MS data. The mass fragmentation patterns of the compounds were recorded and a retrievable in-house library was built to keep the data. In order to demonstrate the potential of this approach, the polyphenolic crude extract was analysed with the LC-MS/MS system and the MS/MS spectra extracted for each chromatographic peak of interest. The fragmentation patterns were compared with those of anticipated pure compounds that were previously recorded. All compounds were successfully identified. It is therefore believed that the LC-MS/MS potential for dereplication of structurally similar compounds in a crude mixture was thus firmly established.

• 한국컴퓨터산업학회논문지
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• 제3권12호
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• pp.1775-1784
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• 2002

지문 인식 기술은 지문 영상의 고유한 특성으로 인하여 생체인식 분야에서 현재 가장 많이 사용되고 있으며 연구도 상당한 진척을 본 것이 사실이다. 하지만 인식 성능 및 일상생활의 활용측면에서는 개발이 완전히 이루어져 있다고는 볼 수 없다. 따라서 본 논문에서는 우리가 흔히 볼 수 있는 무인 정보 단말기의 인증 시스템에 필요한 알고리즘과 환경에 따른 전처리 과정의 다양화 필요성을 인지하고 연구하게 되었다. 이 과정에서 시스템 환경과 지문이미지의 효율적인 획득이 중요한 요소임을 광학식 지문 입력 장치 및 주민등록증에 있는 지문을 스캔하는 방법을 사용하여 증명하고 지문 특징점 검출 및 영상 향상을 위한 기법을 연구하여 정확하고 빠른 계산시간 안에 찾을 수 있는 방법을 제시한다. 또한 전체 시스템의 구성을 위한 효과적인 알고리즘 구현을 목표로 연구한다.

• Journal of Microbiology and Biotechnology
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• 제16권6호
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• pp.855-862
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• 2006

Hexavalent chromium ($Cr^{6+}$) is a highly harmful pollutant, which can be detoxified and precipitated through reduction to $Cr^{3+}$. Bacillus megaterium TKW3 previously isolated from chromium-contaminated marine sediments was capable of reducing $Cr^{6+}$ in concomitance with metalloids ($Se^{4+}$, $Se^{6+}$, and $As^{5+}$). Notwithstanding approximately 50% inhibition, it was the first report of simultaneous bacterial reduction of $Cr^{6+}$ and $Se^{4+}$ (to elemental Se). No significant difference was observed among electron donors (glucose, maltose, and mannitol) on $Cr^{6+}$ reduction by B. megaterium TKW3. The reduction was constitutive and determined to be non-plasmid mediated. Peptide mass fingerprints (PMF) revealed a novel aerobic membrane-associated reductase with $Cr^{6+}$-induced expression and specific reductive activity (in nmol $Cr^{6+}$/mg protein/min) of 0.220 as compared with 0.087 of the soluble protein fraction. Respiratory inhibitor $NaN_3$ did not interfere with the reductase activity. Transmission electron microscopy with energy dispersive X-ray (TEM-EDX) analysis confirmed the aggregation of reduced chromium along the intracellular membrane region. Future identification of the N-terminal amino acid sequence of this reductase will facilitate purification and understanding of its enzymatic action.

• 한국정보통신학회논문지
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• 제9권7호
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• pp.1465-1472
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• 2005

최근 지문, 얼굴 및 홍채 등과 같은 생체정보를 이용하는 정보 전송 및 처리에 관한 연구가 활발하게 진행되고 있다. 지금까지 연구 및 개발된 결과를 볼 때, 많은 생체 정보 시스템이 생체 정보의 가공과 전송에 있어서 개별적인 포맷과 인터페이스를 사용하고 있는 경향이 많다. 이로 인하여 생체 정보의 상호 공유, 호환성 및 상호운용성 등과 같은 문제점이 발생하고 있다. 이러한 문제점을 해결하기 위하여, 본 논문에서는 서버-클라이언트 환경에서 생체정보의 처리 및 전송을 위하여 XML 기술(XCBF, SOAP 등)을 활용하는 연구를 하였으며, 그 결과에 따른 XML 형식의 생체정보 전송 및 처리 방식을 제안한다.

• 통합자연과학논문집
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• 제4권3호
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• pp.238-242
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• 2011

For any pattern matching based algorithm in WLAN environment, the characteristics of signal to noise ratio(SNR) to multiple access points(APs) are utilized to establish database in the training phase, and in the estimation phase, the actual two dimensional coordinates of mobile unit(MU) are estimated based on the comparison between the new recorded SNR and fingerprints stored in database. The system that uses the artificial neural network(ANN) falls in a local minima when it learns many nonlinear data, and its classification accuracy ratio becomes low. To make up for this risk, the SVM/ANN hybrid algorithm is proposed in this paper. The proposed algorithm is the method that ANN learns selectively after clustering the SNR data by SVM, then more improved performance estimation can be obtained than using ANN only and The proposed algorithm can make the higher classification accuracy by decreasing the nonlinearity of the massive data during the training procedure. Experimental results indicate that the proposed SVM/ANN hybrid algorithm generally outperforms ANN algorithm.

• Fisheries and Aquatic Sciences
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• 제4권1호
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• pp.39-46
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• 2001

To examine the effect of copy number-dependent transgenic genotype on the expression of foreign gene, stable hemizygous and homozygous transgenic breeding line was established using artificial parthenogenesis. For this purpose, induced diploid gynogenetic transgenesis was optimized in mud loach (Misgurnus mizolepis) using UV-irradiated cyprinid loach (M. anguillicaudatus) sperm and thermal shocks. Optimum UV range for inactivation of cyprinid loach sperm was between 3,150 to $4,050\;ergs/mm^2$ The UV-irradiated sperm were inseminated into eggs from recessive color strain (yellow) or heterozygous transgenic mud loach containing CAT gene. Cold shock at $2^{\circ}C$ for 60 min, 5 min post fertilization successfully restored the diploidy of eggs inseminated with UV-irradiated sperm. Restoration to diploidy was confirmed by flow cytometry and gynogenetic status was verified by examining maternal exclusive inheritance of multi-locus DNA fingerprints, body color and transgenic marker. Putative isogenic transgenic fish clearly showed homozygous status at trans gene locus based on Southern blot hybridization and progeny testing. Further, such homozygous gynogenetic diploids revealed the increased levels of transgene expression, when compared to those of heterozygous (hemizygous) transgenic fish.

• 한국음향학회지
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• 제32권5호
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• pp.415-422
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• 2013

본 논문에서는 경첩 손실 함수를 최소화를 통해서 강인한 이진 오디오 핑거프린팅 방법을 제안하였다. 특히 제안된 방법에서 오디오 핑거프린트는 이진값을 가지므로 핑거프린트 DB 크기를 줄여줄 수 있는 장점이 있다. 일반적으로 특징을 이진화하는 과정에서 핑거프린트의 강인성, 식별성 등 성능의 손실이 불가피하므로 손실을 최소화하는 것이 필요하다. 본 논문에서는 핑거프린팅에서 두 오디오 클립 간의 유사도가 경첩 함수 형태로 주어지는 것에 착안하여 경첩 손실을 최소화하는 방법으로 특징을 이진화하여 핑거프린트를 구하는 방법을 제안한다. 유도된 경첩 손실 함수는 최소 손실 해싱 기법을 통해서 최소화 하였다. 수 천곡 규모의 오디오에 대해서 다양한 변환들에 대한 인식 성능을 실험하였으며, 제안된 경첩 손실 함수 최소화를 통해서 핑거프린트의 식별성과 강인성이 개선됨을 확인하였다.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.899-903
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• 2005

Two infectious species of Streptococcosis pathogens were detected by multiplex PCR assay. Detection rates of Streptococcus iniae and S. parauberis could reach 44.9% and 55.1% respectively for one year during 2004 to 2005 in Jeju island. These findings showed that S. parauberis strains were important pathogen with streptococcosis of olive flounder in Jeju island. These findings showed that S. parauberis strains were important pathogen with streptococcosis of olive flounder in Jeiu island. In the present study we have investigated the interspecific relationship of all Jeju area of S. parauberis by RAPD analysis. Represent strains divided to four groups by RAPD fingerprints. The important differences observed between the olive flounder isolates suggest that they could constitute a well-differentiated group or a separate clonal line within this bacterial species. Though, serological research of S. parauberis strains in Jeju island not exist yet. These strains doing the serological evolution.

• 제어로봇시스템학회논문지
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• 제20권7호
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• pp.774-781
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• 2014

This paper presents a fingerprint database construction method for WLAN RSSI (Received Signal Strength Indicator)-based indoor positioning. When RSSI is used for indoor positioning, the fingerprint method can achieve more accurate positioning than trilateration and centroid methods. However, a FD (Fingerprint Database) must be constructed before positioning. This step is a very laborious process. To reduce the drawbacks of the fingerprint method, a radio propagation model-based FD construction method is presented. In this method, an FD can be constructed by a simulator. Experimental results show that the constructed FD-based positioning has a 3.17m (CEP) error. In this paper, a spatial correlation method is presented to estimate the NLOS(Non-Line of Sight) error included in the FD constructed by a simulator. As a result, the NLOS error of the FD is reduced and the performance of the error compensated FD-based positioning is improved. The experimental results show that the enhanced FD-based positioning has a 2.58m (CEP) error that is a reasonable performance for indoor LBS (Location Based Service).

• 한국전자거래학회지
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• 제21권4호
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• pp.41-53
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• 2016

요즘 각 스마트폰 제조사에서 플래그쉽(flagship) 스마트폰 모델에 지문, 음성, 홍채인식 기능을 기본적으로 탑재하면서 생체인증을 활용한 인증수단이 활성화 되고 있다. 이러한 생체인증(지문, 음성, 홍채 등)은 패턴이나, 비밀번호와 같이 스마트폰의 잠금 해제 기능뿐만 아니라 금융권을 중심으로 다양한 인증수단으로 확산되고 있다. 본 논문에서는 생체인증을 통해서 전자금융(인터넷뱅킹, 스마트뱅킹 등)거래 시 사용자를 인증하고, 거래 내역에 대한 전자서명을 통한 부인방지 기술에 대해 설명하고, 이러한 생체인증 기술이 금융서비스에 접목되기 위해 필요한 관련 기술적, 법률적인 요구사항을 연구하고자 한다.