• Journal of Advanced Marine Engineering and Technology
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• 제40권1호
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• pp.62-68
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• 2016

실내 위치인식 기술 중 하나인 WiFi Fingerprint는 기존의 WiFi access point(AP)의 거리에 따른 신호 세기를 활용하여 위치를 추정하는 편리함 때문에 많은 연구가 이루어지고 있다. 하지만 이 방식은 Radio map에 저장된 Reference point에 의존하기 때문에 다른 방식에 비해 위치의 분해능이 떨어지고 연산량이 많다. 본 논문에서는 이러한 문제를 해결하기 위하여 WiFi와 BLE를 융합한 Log-Distance Path Loss Model 기반의 Radio map 설계 알고리즘을 제안한다. 제안한 알고리즘은 Log-Distance Path Loss Model이 적용된 변수 값을 추출하여 Radio map을 설계하는 방식이며 Median Filter를 적용하여 오차를 개선하였다. 기존 Fingerprint와 비교하여 실험한 결과, 위치의 정확도는 평균 2.747m에서 2.112m로 0.635m 감소되는 것을 확인하였으며 연산량은 AP 환경에 따라 33%이상 감소하는 것을 확인하였다.

• 분석과학
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• 제33권2호
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• pp.98-107
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• 2020

Methamphetamine (MA) is currently the most abused illicit drug in Korea. MA is produced by chemical synthesis, and the final target drug that is produced contains small amounts of the precursor chemicals, intermediates, and by-products. To identify and quantify these trace compounds in MA seizures, a practical and feasible approach for conducting chromatographic fingerprinting with a suite of traditional chemometric methods and recently introduced machine learning approaches was examined. This was achieved using gas chromatography (GC) coupled with a flame ionization detector (FID) and mass spectrometry (MS). Following appropriate examination of all the peaks in 71 samples, 166 impurities were selected as the characteristic components. Unsupervised (principal component analysis (PCA), hierarchical cluster analysis (HCA), and K-means clustering) and supervised (partial least squares-discriminant analysis (PLS-DA), orthogonal partial least squares-discriminant analysis (OPLS-DA), support vector machines (SVM), and deep neural network (DNN) with Keras) chemometric techniques were employed for classifying the 71 MA seizures. The results of the PCA, HCA, K-means clustering, PLS-DA, OPLS-DA, SVM, and DNN methods for quality evaluation were in good agreement. However, the tested MA seizures possessed distinct features, such as chirality, cutting agents, and boiling points. The study indicated that the established qualitative and semi-quantitative methods will be practical and useful analytical tools for characterizing trace compounds in illicit MA seizures. Moreover, they will provide a statistical basis for identifying the synthesis route, sources of supply, trafficking routes, and connections between seizures, which will support drug law enforcement agencies in their effort to eliminate organized MA crime.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.120.2-120
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• 2003

Agrobacterium vitis is a causal agent of crown-gall disease on grapevine. In Korea, grapevine variety (GeoBong) have severely been infected by the bacteria since stems of the variety were buried in soil for overwintering. Infection ratio over 70-80％ was observed on 7 years old GeoBong grapevine in Ansung and Cheonan. PCR specific primers for A. vitis strains were designed using nucleotide sequences of vir A gene in Ti-Plasmid, pheA gene in chromosomal DNA and a URP-PCR polymorphic band. Three hundred bacterial strains were isolated from the different 80 galls formed on GeoBong grapevine in Cheonan and Ansung of Korea and were screened to identify A. vitis using the three specific PCR primers for Agrobacterium vitis. Twenty-four bacterial strains that are detected by the primers were further confirmed by pathogenicity and biochemical methods. To investigate the genomic diversity of the bacterial strains, twenty primers of 20 mer referred to universal rice primers (URP) were applied for PCR fingerprinting, Of them, URP2R and URP2F primers could effectively be used to detect polymorphism within the bacterial strains.

• Tuberculosis and Respiratory Diseases
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• 제77권4호
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• pp.172-177
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• 2014

Background: Pseudomonas aeruginosa infection is particularly associated with progressive and ultimately chronic recurrent respiratory infections in chronic obstructive pulmonary disease, bronchiectasis, chronic destroyed lung disease, and cystic fibrosis. Its treatment is also very complex because of drug resistance and recurrence. Methods: Forty eight cultures from 18 patients with recurrent P. aeruginosa pneumonia from 1998 to 2002 were included in this study. Two or more pairs of sputum cultures were performed during 2 or more different periods of recurrences. The comparison of strains was made according to the phenotypic patterns of antibiotic resistance and chromosomal fingerprinting by pulsed field gel electrophoresis (PFGE) using the genomic DNA of P. aeruginosa from the sputum culture. Results: Phenotypic patterns of antibiotic resistance of P. aeruginosa were not correlated with their prior antibiotic exposition. Fifteen of 18 patients (83.3%) had recurrent P. aeruginosa pneumonia caused by the strains with same PFGE pattern. Conclusion: These data suggest that the most of the recurrent P. aeruginosa infections in chronic lung disease occurred due to the relapse of prior infections. Further investigations should be performed for assessing the molecular mechanisms of the persistent colonization and for determining how to eradicate clonal persistence of P. aeruginosa.

• 정보보호학회논문지
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• 제23권4호
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• pp.617-627
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• 2013

NFC, RFID 등의 무선 기술이 발전하면서 기기간의 데이터 전달이 용이해지고 있다. 사용자는 여러 개의 복잡한 비밀번호를 외우고 입력하는 대신 항상 소지하고 있는 카드나 휴대폰을 이용하여 간편하게 자신을 인증함으로써 건물에 출입하거나, 보안 자료에 접근하고, 결제를 할 수 있게 되었다. 그러나 최근 릴레이 공격(relay attack)의 출현으로 편리한 토큰 기반(something you have) 인증의 안전성이 위협받고 있다. 릴레이 공격은 안전한 통신 채널을 가진 두 기기 사이에서도 효과적으로 공격을 성공시킬 수 있고, 공격의 원리가 복잡하지 않아 쉽게 구현이 가능하다. 본 논문에서는 거리 제한 방식(distance bounding)이나 위치 측정 후 비교와 같은 기존 방어 기법과 다른 청각 채널을 통한 릴레이 공격 탐지에 대해 제안한다.

• Journal of Microbiology and Biotechnology
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• 제14권3호
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• pp.599-606
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• 2004

Gram-positive antagonistic bacilli were isolated from agricultural soils for possible use in biocontrol of plant pathogenic fungi, Fusarium oxysporum, Rhizoctonia solani, and/or Pythium ultimum. Among the 65 antagonistic Gram-positive soil isolates, 22 strains were identified as Bacillus species by 16S rDNA sequence analyses. Four strains, including DF14, especially exhibited multiple antagonistic properties against the three damping-off fungi. Genotypic properties of the Bacillus isolates were characterized by rapid molecular fingerprinting methods using repetitive extragenic palindromic-PCR (REP-PCR), ribosomal intergenic spacer-length polymorphisms (RIS-LP), 16S rDNA PCR-restriction fragment length polymorphisms (PCR-RFLP), and strain-specific PCR assays. The results indicated that the REP-PCR method was more valuable than the RIS-LP and 16S rDNA PCR-RFLP analyses as a rapid and reliable approach for bacilli typing and identification. The use of strain-specific primers designed based on 16S rDNA sequence comparisons enabled it to be possible to selectively detect a strain, DF14, which is being used as a biocontrol agent against damping-off fungi.

• 한국동물위생학회지
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• 제32권3호
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• pp.257-264
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• 2009

Subtyping of Brucella abortus isolates is epidemiologically important for monitoring of bovine brucellosis outbreaks. Pulsed-field gel electrophoresis (PFGE) is considered as a gold standard of molecular typing methods to study the DNA polymorphisms of bacteria. In this study, we analyzed using PFGE the DNA fragment profiles of B. abortus isolated in Gyeongbuk province from 1998 to 2006. The genomic DNA was digested with the restriction endonuclease Xba I, Xho I and Smi I followed gel electrophoresis. No distinguishable patterns of the genomic DNA digested with Xba I and Xho I were observed among the field isolates of B. abortus tested in this study. But Smi I restriction enzyme resulted in two PFGE patterns consisting of 13-15 bands that ranged in size from 33 to 668bp by standard marker. The cluster analysis by DNA fingerprinting software showed 93.75% similarity between two PFGE patterns. No different PFGE patterns were recognized among the isolates originated from various years, regions and cow breeds.

• Genomics & Informatics
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• 제11권3호
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• pp.102-113
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• 2013

Metagenomics has become one of the indispensable tools in microbial ecology for the last few decades, and a new revolution in metagenomic studies is now about to begin, with the help of recent advances of sequencing techniques. The massive data production and substantial cost reduction in next-generation sequencing have led to the rapid growth of metagenomic research both quantitatively and qualitatively. It is evident that metagenomics will be a standard tool for studying the diversity and function of microbes in the near future, as fingerprinting methods did previously. As the speed of data accumulation is accelerating, bioinformatic tools and associated databases for handling those datasets have become more urgent and necessary. To facilitate the bioinformatics analysis of metagenomic data, we review some recent tools and databases that are used widely in this field and give insights into the current challenges and future of metagenomics from a bioinformatics perspective.

• 동의생리병리학회지
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• 제30권6호
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• pp.397-401
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• 2016

The quality control is a fundamental procedure for the standardization of herbal medicine to guarantee the consistency of efficacy and safety. For a long time, the quality analysis of herbal medicine has been largely dependent on the routine sensory evaluation, such as taste, smell, color, and shape. However, with the recent development of analytical instruments, various scientific approaches have been introduced in this field. On the basis of the theory that the biological activities of herbal medicine are mainly contributed by its chemical compositions, several types of chemical markers have been suggested for the quality evaluation. In addition to the analytical methods for the specific marker compound(s), including analytical marker and active marker, recently, chemical fingerprinting, a method comparing the chromatographic pattern of the whole chemical components, has been developed and widely accepted as a reliable approach for the quality control of herbal medicine. Moreover, in order to exactly understand the relationship between complex compounds and their holistic biological activities in herbal medicine, unique strategies, such as "BECCs (bioactive equivalent combinatorial components)" and "PhytomicsQC" have been established. In this article, we give an overview of the several categories of chemical markers and the recent research trends for the quality evaluation of herbal medicine.

• 한국정보과학회논문지:데이타베이스
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• 제41권4호
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• pp.199-208
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• 2014

본 논문에서는 스캔된 만화의 콘텐츠 특성을 고려한 식별 및 특징 검색 시스템을 제안하였다. 스캔 만화의 특징점을 생성하기 위해서 계층적 대칭 핑거프린팅 방법을 활용하였다. 제안하는 핑거프린트 식별 및 검색 시스템은 웹하드와 같은 온라인 서비스 제공자들이 대량의 스캔만화에 대하여 즉각적인 식별 결과를 얻을 수 있도록 설계되었다. 실험에서는 회전, 이동 등의 이미지 변형에 대해서 핑거프린트의 식별 강인성에 대하여 분석하였다. 또한 특징점 데이터베이스에서의 빠른 매칭을 위한 데이터베이스 구조를 제안하였고, 전역 검색 및 최대중요특징 검색과 같은 기존의 다른 검색방법과 성능을 비교하였다.