• 제목/요약/키워드: Finger Millet

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Profile of phenolic compounds, antioxidant and SOD activity of millet germplasm

  • Lee, Myung-Chul;Choi, Yu-Mi;Yun, Hyemyeong;Hyun, Do-Yoon;Lee, Sukyeung;Oh, Sejong
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2019년도 춘계학술대회
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    • pp.107-107
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    • 2019
  • Millets are provided considerable amounts of nutrients and gluten-free cereal products and their rich non-nutritional compounds having proven health benefits, especially phenolic compounds. The aim of present investigation was to determine phenolic composition and antioxidant and SOD activity of three different millet of genetic resources namely, foxtail, proso and finger millet. Phenolic compounds were extracted from dehulled grain of genetic resources using methanol and examined for their total phenolic content (TPC), antioxidant activities and superoxide dismutase (SOD)-like activity. The TPC range of hog millet, finger millet and finger millet range from 3.3 to 25.1, 11.1 to 29.0 and 3.8 to 94.3 gallic acid equivalent (GAE)mg/g, respectively. Most of TCP content in dehulled millet grains was distributed from 10 to 20 gallic acid equivalent (GAE)/g, but two accessions of finger millet (IT235690 and 235689) were showed over than 90. The antioxidant activities were measured by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity. Finger millet and hog millet showed 26.4% and 26.7% in the mean of DPPH scavenging activity percentage, but foxtail millet was 13%. The finger millet showed the higher value than hog and foxtail millet in superoxide dismutase (SOD)-like activity. Particularly, two accessions of finger millet (IT235690 and 235689) showed the highest phenolic content and antioxidant activities among the used millet genetic resources and will be primary resources for finger millet breeding to develop the appropriate breeding strategies.

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Digestibility of Amino Acids of Maize, Low Tannin Sorghum, Pearl Millet and Finger Millet in Caecectomized Roosters

  • Vasan, P.;Mandal, A.B.;Dutta, Narayan;Maiti, S.K.;Sharma, K.
    • Asian-Australasian Journal of Animal Sciences
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    • 제21권5호
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    • pp.701-706
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    • 2008
  • The aim of the present study was to determine the apparent and true digestibilities of amino acids of maize, low tannin sorghum, pearl millet and finger millet in adult caecectomized cockerels. Adult cockerels (n = 60), 25-weeks old, were used in this study of which 30 birds were caecectomized as per a standard method. The apparent digestibilities of amino acids of sorghum were not affected by caecectomy, but were higher for maize, finger millet and pearl millet in caecectomized cockerels. Caecectomy had no influence on the true digestibilities of amino acids of maize, but higher digestibilities were observed for most of the amino acids of sorghum and finger millet in caecectomized cockerels. Caecectomy lowered the true digestibility of cystine, threonine and serine of pearl millet. The apparent digestibilities of amino acids of maize, finger millet and pearl millet were underestimated in intact cockerels. The true digestibilities of most of the amino acids of sorghum and finger millet were underestimated, while those of cystine, threonine and serine of pearl millet were overestimated in intact cockerels. The findings suggest that the amino acid digestibility values of cereal grains determined using caecectomized cockerels might be appropriate and reliable for poultry diet formulations. Moreover, the digestibilities of amino acids of finger millet were inferior to other cereal grains, while those of pearl millet were comparable to maize and sorghum.

Macro- and Micro-nutrient Utilization and Milk Production in Crossbred Dairy Cows Fed Finger Millet (Eleucine coracana) and Rice (Oryza sativa) Straw as Dry Roughage Source

  • Gowda, N.K.S.;Prasad, C.S.
    • Asian-Australasian Journal of Animal Sciences
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    • 제18권1호
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    • pp.48-53
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    • 2005
  • Finger millet straw and rice straw are the major source of dry roughage in southern India. They distinctly vary in their morphological and nutritional characters. Hence an effort was made to study the nutrient utilization, milk yield and composition in crossbred dairy cows fed either finger millet (group 1) or rice straw (group 2) as a source of dry roughage. The cows in both the groups were fed as per requirement with concentrate, green fodder and straw in the ratio of 30:45:25 parts (DM). At the end of 50 days of preliminary feeding a digestibility trial was conducted for 7 days and pooled samples of feed, fodder, feces, urine and milk were analysed for macro and micro nutrient content. Finger millet straw contained more CP, Ca, P, Mg, Cu, Zn and Co than rice straw and rice straw contained higher ADF, ash and silica. The intake of DM, CP, EE, NDF, ADF and most micronutrients (Ca, P, Mg, Cu, Zn, Fe, Mn and Co) was significantly higher in cows fed finger millet straw. The digestibility of DM, CP, NDF and ADF was significantly higher in cows fed finger millet straw and the gut absorption of Ca, Cu, Mn and Co was significantly higher in cows fed finger millet straw. The dietary requirement of all micronutrients in both the group of cows could be met irrespective of the type of roughage fed except that of Ca, which was low (0.61 and 0.40%) in rice straw fed cows. The average daily milk yield (L/cow) was also higher (7.0 L) in cows fed finger millet straw as compared to cows fed rice straw (6.3 L). The average milk composition also did not differ except that of milk fat which was significantly (4.7 and 4.5%) low in cows fed rice straw. The overall results of this study have indicated that finger millet straw is a better source of dry fodder than rice straw and while feeding rice straw as the sole roughage to dairy cows there is need to supplement additional calcium as this could be one of the limiting nutrients for milk production.

Development of SSR Markers and Their Use in Studying Genetic Diversity and Population of Finger Millet (Eleusine coracana L. Gaertn.)

  • Lee, Kyung Jun;Yoon, Mun-Sup;Shin, Myoung-Jae;Lee, Jung-Ro;Cho, Yang-Hee;Lee, Ho-Sun;Ma, Kyung-Ho;Lee, Gi-An
    • Plant Breeding and Biotechnology
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    • 제5권3호
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    • pp.183-191
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    • 2017
  • Finger millet (Eleusine coracana L. Gaertn.) is an important cereal crop in eastern Africa and southern India with excellent grain storage capacity and the unique ability to thrive in extreme environmental conditions. In this study, we analyzed the genetic diversity and population structure of finger millet using 12 developed microsatellites. By sequencing 815 clones from an SSR-enriched genomic DNA library, we obtained 12 polymorphic SSR markers, which also revealed successful amplicons in finger millet accessions. Using the developed SSR markers, we estimated genetic diversity and population structure among 76 finger millet accessions in Asia, Africa, and unknown origins. The number of alleles ranged from 2 to 9, with an average of 3.3 alleles. The mean values of observed heterozygosity and expected heterozygosity were 0.27 and 0.35, respectively. The average polymorphism information content was 0.301 in all 76 finger millet accessions. AMOVA analysis showed that the percentage of molecular variance among the populations was 1%, that among individuals was 5%, and that within individuals was 94%. In STRUCTURE analysis, the 76 finger millet accessions were divided into two subpopulations which had an admixture of alleles. There was a correspondence among PCoA, AMOVA, and population structure. This study may form the basis for a finger millet breeding and improvement program.

Development of EST-SSRs and Assessment of Genetic Diversity in Germplasm of the Finger Millet, Eleusine coracana (L.) Gaertn.

  • Wang, Xiaohan;Lee, Myung Chul;Choi, Yu-Mi;Kim, Seong-Hoon;Han, Seahee;Desta, Kebede Taye;Yoon, Hye-myeong;Lee, Yoonjung;Oh, Miae;Yi, Jung Yoon;Shin, Myoung-Jae;Kim, Kyung-Min
    • 한국작물학회지
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    • 제66권4호
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    • pp.443-451
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    • 2021
  • Finger millet (Eleusine coracana) is widely cultivated in tropical regions worldwide owing to its high nutritional value. Finger millet is more tolerant against biotic and abiotic stresses such as pests, drought, and salt than other millet crops; therefore, it was proposed as a candidate crop to adapt to climate change in Korea. In 2019, we used expressed sequence tag simple sequence repeat (EST-SSR) markers to evaluate the genetic diversity and structure of 102 finger millet accessions from two geographical regions (Africa and South Asia) to identify appropriate accessions and enhance crop diversity in Korea. In total, 40 primers produced 116 alleles, ranging in size from 135 to 457 bp, with a mean polymorphism information content (PIC) of 0.18225. Polymorphism was detected among the 40 primers, and 13 primers were found to have PIC values > 0.3. Principal coordinate and phylogenetic analyses, based on the combined data of both markers, grouped the finger millet accessions according to their respective collection areas.Therefore, the 102 accessions were classified into two groups, one from Asia and the other from Africa. We have conducted an in-depth study on the finger millet landrace pedigree. By sorting out and using the molecular characteristics of each pedigree, it will be useful for the management and accession identification of the plant resource. The novel SSR markers developed in this study will aid in future genetic analyses of E. coracana.

칼슘 함량이 높은 손가락조 '핑거1호'와 그 가공품의 영양 및 품질 특성 (Evaluation of Nutritional Content and Quality Attributes of Cookies Utilizing Calcium-Enriched Finger Millet Variety (Finger1ho))

  • 추지호;고지연;최명은;김지영;이병원;주영광;서효섭;김춘송;한상익
    • 한국작물학회지
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    • 제68권4호
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    • pp.422-430
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    • 2023
  • 우리나라 국민들에게 부족하기 쉬운 영양소인 칼슘함량이 풍부하고 항산화 활성이 높은 손가락조의 기능성 식품소재로서의 가능성을 확인하고자 손가락조 '핑거1호'를 이용하여 쿠키 가공을 하였을 때 품질 특성 및 칼슘 함량 등의 변화를 평가하여 신작물인 손가락조를 이용한 가공품 개발에 활용 여부를 판단하였다. 1) 밀가루 100%를 대조로하여 핑거1호 통곡 분말 10%, 30%, 50%, 100%를 첨가하여 쿠키를 제조하고 가공 특성과 이화학 특성 등, 품질과 항산화 활성 등을 조사하였다. 2) 핑거1호 분말 첨가량이 증가함에 따라 칼슘 함량은 10% 첨가시 1.8배에서 100%시 10.8배에 이르기까지 현저하게 증가하였다. 3) 반면, 나트륨의 경우 10% 첨가시 1.01배에서 100%시 1.38배로 증가량의 변화가 크지 않아 칼슘이 부족하고 나트륨은 과다섭취하는 우리나라 국민들의 식습관을 고려할 시 영양학적으로 바람직한 쿠키의 제조가 가능할 것으로 판단되었다. 4) 항산화활성도 칼슘 등 무기성분 함량증가와 마찬가지로 핑거1호 첨가량의 증가에 ABTS radical 소거활성 1.6~7.5배, DPPH radical 소거활성 2.8~18.7배 증가하였다. 5) 쿠키의 품질특성에서 핑거1호 분말첨가가 증가할수록 쿠키의 퍼짐성은 증가하고 경도가 감소되어 부드러운 조직감의 쿠키제조가 가능하였고, 색상도 10%의 첨가만으로도 뚜렷한 차이를 나타내었다.

Genetic Diversity of Finger Millet (Eleusine coracana (L.) Gaertn.) Landraces Based on EST-SSR

  • Myung Chul Lee;Yu-Mi Choi;Myoung-Jae Shin;Hyemyeong Yoon;Seong-Hoon Kim
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2020년도 춘계학술대회
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    • pp.46-46
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    • 2020
  • Finger millet is more nutritious than other and millets and widely cultivate in tropical regions of the world. Furthermore, it is more tolerant against biotic and abiotic stresses such as pest, drought and salt. For this reason, finger millet is one of the putative crops to introduce and cultivate on reclaimed land and prepare the global climate exchange in Korea. In present study, genetic diversity and structure of different populations of finger millet from Africa and South Asia was examined at molecular level using newly developed EST-Simple Sequence Repeat (EST-SSR) markers. In total, 46 primers produced 292 alleles in a size range of 100-500 bp and mean Polymorphism Information Content (PIC) and Marker Index (MI) were 0.372 and 1.04, respectively. 46 primers showed polymorphism and 21 primers were identified as having a PIC value above 0.5. Principal coordinates analysis and the dendrogram constructed out of combined data of both markers showed grouping of finger millet accessions to their respective area of collection. The 156 accessions were more classified into four groups, such as three groups of Africa collection and one group of Asia. Results of present study can be useful in identifying diverse accessions and management of this plant resource. Moreover, the novel SSR markers developed can be utilized for various genetic analyses in this species in future.

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Replacement of Yellow Maize with Pearl Millet (Pennisetum typhoides), Foxtail Millet (Setaria italica) or Finger Millet (Eleusine coracana) in Broiler Chicken Diets Containing Supplemental Enzymes

  • Rama Rao, S.V.;Raju, M.V.L.N.;Reddy, M.R.;Panda, A.K.
    • Asian-Australasian Journal of Animal Sciences
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    • 제17권6호
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    • pp.836-842
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    • 2004
  • An experiment was conducted to study the performance of broilers chicks (2 to 42 d of age) fed diets containing pearl millet (PM, Pennisetum typhoides), foxtail millet (FOM, Setaria italica) or finger millet (FIM, Elusine coracana) totally replacing (w/w) yellow maize (YM) with and with out supplementing non-starch polysaccharide (NSP) hydrolysing enzymes at the rate of 0.5 g/kg diet. Enzyme preparation contained amylase 2,400 units, hemi-cellulase 5,400 units, cellulase 12,000 units, protease 2,400 units and beta-glucanase 106 units/g. Each diet was fed to eight replicates (five female Vencob broilers/replicate) housed in stainless steel battery brooders. The estimated metabolizable energy (ME) contents of YM, PM, FOM and FIM were FM (PM) were about 3,389, 2,736, 3,303 and 2,846 kcal/kg, respectively. Total replacement of YM with FOM did not influence the body weight gain, ready to cook yield, relative weights of giblet, liver, intestine, lymphoid organs (bursa and spleen) and length of intestine, antibody titers and livability at 42 d of age. But the food efficiency decreased significantly in FOM fed broilers compared those fed YM. Further, the fat content in thigh muscle reduced with FOM fed groups compared to those fed YM. The performance of broilers decreased significantly in PM and FIM fed broilers compared to those fed YM. The relative weights of giblet, gizzard and liver increased in FIM fed groups compared to those fed YM as the principal source of energy in broilers. Incorporation of NSP hydrolysing enzymes in commercial broiler diets improved the efficiency of feed utilization during starter phase but not at 42 d of age. The results thus indicate that yellow maize can be replaced in toto on weight basis in commercial broiler diets without affecting the performance. Supplementation of NSP hydrolysing enzymes was beneficial in enhancing feed utilization during the starter phase.

Development of EST-SSR Markers for Evaluation of Genetic Diversity and Population Structure in Finger Millet (Eleusine coracana (L.) Gaertn.)

  • Lee, Myung Chul;Choi, Yu-Mi;Hyun, Do-Yoon;Lee, Sukyeung;Kim, Jin-Hee;Oh, Sejong
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2018년도 춘계학술발표회
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    • pp.105-105
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    • 2018
  • Finger millet, Eleusine coracana Gaertn., is more nutritious than other cereals and millets and widely cultivate in tropical regions of the world. However, status of its genetic diversity remained concealed due to lack of research work in this species. In recent years, microsatellites have become the most used markers for studying population genetic diversity. In present study, genetic diversity and structure of different populations of finger millet from Africa and South Asia was examined at molecular level using newly developed EST-Simple Sequence Repeat (EST-SSR) markers using a total of 1,927 ESTs of Eleusine coracana available in the NCBI database. In total, 46 primers produced 292 alleles in a size range of 100-500 bp and mean Polymorphism Information Content (PIC) and Marker Index (MI) were 0.372 and 1.04, respectively. 46 primers showed polymorphism and 21 primers were identified as having a PIC value above 0.5. Principal coordinates analysis and the dendrogram constructed out of combined data of both markers showed grouping of finger millet accessions to their respective area of collection. The 156 accessions was classified into four groups, such as three groups of Africa collection and one group of Asia. Results of present study can be useful in identifying diverse accessions and management of this plant resource. Moreover, the novel SSR markers developed can be utilized for various genetic analyses in this species in future.

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Antioxidant and Antihypertensive Activities of Grains Grown in South Korea in Relation to Phenolic Compound and Amino Acid Contents

  • Narae Han;Koan Sik Woo;Jin Young Lee;Jiho Chu;Mihyang Kim;Yu-Young Lee;Moon Seok Kang;Hyun-Joo Kim
    • 한국식품영양학회지
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    • 제36권6호
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    • pp.572-580
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    • 2023
  • Hypertension is characterized by excessive renin-angiotensin system activity, leading to blood vessel constriction. Several synthetic compounds have been developed to inhibit renin and angiotensin-converting enzyme (ACE). These drugs often have adverse side effects, driving the exploration of plant protein-derived peptides as alternative or supplementary treatments. This study assessed the phenolic compound and amino acid content and the antioxidant and antihypertensive activity of 5 South Korean staple crops. Sorghum had the highest phenolic compound content and exhibited the highest antioxidant activity. Millet grains, particularly finger millet (38.86%), showed higher antihypertensive activity than red beans (14.42%) and sorghum (17.16%). Finger millet was found to contain a large proportion of branched-chain, aromatic, and sulfur-containing amino acids, which are associated with ACE inhibition. In particular, cysteine content was positively correlated with ACE inhibition in the crops tested (r=0.696, p<0.01). This study confirmed that the amino acid composition was more correlated with the antihypertensive activity of grains than the phenolic compound content. Finger millet mainly contained amino acids, which have higher ACE inhibitory activity, resulting in the strongest antihypertensive activity. These findings underscore the antihypertensive potential of select crops as plant-based food ingredients, offering insight into their biological functions.