• Applied Microscopy
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• 제23권2호
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• pp.97-106
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• 1993

This study was done to elucidate the electron microscopic characteristics of certain pathogenic fungi. Four Candida species, (C. albicans, C. tropicalis, C. parapsilosis and C. glabrate) and Cryptococcus neoformans were cultured for 3 days at $30^{\circ}C$ in the Sabouraud dextrose medium. After incubation, they were stored at $4^{\circ}C$ for 24hours. Fine structures were analyzed by morphometry, and Tukey's HSD test was used for statistics. On scanning electron microscopy C. albicans and C. neoformans were similar in size but different in shape, showing sphero-shape or ovalo-shape in C. neoformans. Surface of C. neoformans was coarse and spiny, but Candida species examined were uniformly smooth. In size, C. glabrata was the smallest among them. Budding scar as seen on the surface of Candida species by the number ranging from 1 to 7. Cryptococcus neoformans showed one or two budding scar. On transmission electron microscopy the cytoplasm of most yeast cells showed plentiful glycogen particles, mitochondria, peroxisomes and vacuoles. However, cell walls were different among four Candida species and Cryptococcus neoformans. The cell wall of Candida species consisted of fibrous layer, that was electron dense layer and transparent layer, in contrast to Cryptococcus neoformans consisted of electron dense layer with lamellar structure. This layer was two times thicker than that of Candida species. The outer layer of cell wall was of radiating pattern.

• 한국의류학회지
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• 제40권6호
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• pp.1025-1033
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• 2016

It is important to compare and analyze digital camouflage from different countries to promote the continuous development of a camouflage combat uniform. This effort should lead to developing a camouflage pattern suitable for the domestic environment and expand its performance of night camouflage. This study investigates digital camouflage by comparing camouflage fabrics sampled from Korea and eight other countries (USA, UK, Singapore, Croatia, Colombia, and Mongolia) in terms of textile, near-infrared reflectivity of colors, and color distribution. First, the fabric construction of camouflage from Korea, UK, US, Singapore, Span, and Croatia were all characterized by derivative plain weaves, while derivative twill weaves were generally used in Croatia and Mongolia. It is assumed that derivative plain weaves are adopted to improve the tearing strength of fine yarns. However, twill weaves enhance the flexibility of coarse yarn fabrics. Next, reflectivity change was analyzed based on camouflage color. The reflectivity of a combat uniform in Korea, Colombia, Croatia, and UK increased before 780nm in the visible light range, but remained consistent from 800nm which falls under the near-infrared range. In contrast, camouflage samples in Mongolia, Span, Singapore and USA showed a gradual increase of reflectivity in the near-infrared range. Finally, the color distribution analysis of digital camouflage found that camouflage of countries with desert or woodland combat settings dominantly contained brown colors. It indicates the color pattern consideration of different geographic regions is important to determine camouflage performance. This research involves basic study that will have implications for developing patterns and colors suitable for the South Korean environment and expand its use as night camouflage that helps achieve continuous improved camouflage performance.

• Asian Pacific Journal of Cancer Prevention
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• 제17권6호
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• pp.2909-2916
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• 2016

Background: Canine malignant lymphoma is classified into B- or T-cell origin, as in the human case. Due to differences in prognosis, a suitable method needs to be developed for lineage identification. Aims: To determine the accuracy of immunophenotypic and molecular information between three methods: immunocytochemistry (ICC), immunohistochemistry (IHC) and heteroduplex polymerase chain reaction for antigen receptor rearrangements (hPARR) in spontaneous canine lymphomas. Materials and Methods: Peripheral blood, fine needle aspiration and tissue biopsies from enlarged peripheral lymph nodes prior to treatment of 28 multicentric lymphoma patients were collected. Cytopathology and histopathology were examined and classified using the updated Kiel and WHO classifications, respectively. Anti-Pax5 and anti-CD3 antibodies as B- and T-cell markers were applied for immunophenotyping by ICC and IHC. Neoplastic lymphocytes from lymph node and white blood cell pellets from peripheral blood were evaluated by hPARR. Results: In this study, low grade B-cell lymphoma accounted for 25% (7/28), high grade B-cell lymphoma for 64.3% (18/28) and high grade T-cell lymphoma for 10.7% (3/28). According to the WHO classification, 50% of all cases were classified as diffuse large B-cell lymphoma. In addition, ICC showed concordant results with IHC; all B-cell lymphomas showed Pax5+/CD3, and all T-cell lymphomas exhibited Pax5-/CD3+. In contrast to hPARR, 12 B-cell lymphomas featured the IgH gene; seven presented the $TCR{\gamma}$ gene; five cases showed both IgH and $TCR{\gamma}$ genes, and one case were indeterminate. Three T-cell lymphomas showed the $TCR{\gamma}$ gene. The percentage agreement between hPARR and ICC/IHC was 60%. Conclusions: Immunophenotyping should not rely on a single method. ICC or IHC with hPARR should be used concurrently for immunophenotypic diagnosis in canine lymphomas.

• 한국차세대컴퓨팅학회논문지
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• 제14권5호
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• pp.53-59
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• 2018

최근 컴퓨터 비전 기술의 발전으로 스마트도시에서는 합리적인 정확도로 복잡한 동작을 인식할 수 있다. 이와는 대조적으로, 싸움과 칼에 관련된 사건과 같은 폭력적인 인식은 관심을 덜 이끌었다. 시각적인 감시 능력은 거리나 교도소에서의 싸움을 감지하는데 사용될 수 있다. 이 논문에서 우리는 감시 카메라에 대한 심층 학습 기반의 폭력 인식 방법을 제안했다. 컨볼루션 뉴럴 네트워크(CNN) 모델은 폭력 인식을 위한 싸움과 칼의 벤치마크 데이터 셋에 대해 훈련하고 세부적으로 조정된다. 비정상적인 이벤트가 감지되면 가장 가까운 경찰서로 경보를 보내는 즉각적인 조치를 취할 수 있다. 제안된 방법의 실험 결과는 99.21%의 정확도를 달성함으로써 다른 최첨단 CNN모델을 능가했다.

• 한국항공우주학회지
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• 제50권1호
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• pp.39-45
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• 2022

본 논문에서는 타겟과의 거리 및 EO/IR 장비 내부 온도정보를 이용한 전통적인 방식의 초점조절 방식의 단점을 보완할 수 있는 향상된 영상기반 초점 조절 기법을 제안하였다. 영상기반 초점조절시 카메라시선의 흔들림에 의해 동일한 표적으로 시선을 지향하지 못해 영상의 다른 영역에 초점 윈도우를 설정함으로 인해 정확한 초점 값을 계산할 수 없게 된다. 이러한 문제를 해결하기 위해 영상추적 기법을 적용하여 동일한 위치에 포커스 윈도우가 설정되도록 하여 최적의 초점위치 탐색 정확도를 향상시키고자 하였다. 또한 동일한 초점위치에서 플랫폼의 진동이나 흔들림 그리고 대기환경에 의한 영상 불안정으로 인해 동일한 초점 값이 계산되지 않기 때문에 인접프레임 중에 보다 안정적인 상태에서 획득된 프레임을 선택함으로써 국부최대값(Lcoal maxima) 빠지지 않고 최적의 초점 위치를 찾는 기법을 제안하였다.

• Molecules and Cells
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• 제45권8호
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• pp.575-587
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• 2022

Human ABCB6 is an ATP-binding cassette transporter that regulates heme biosynthesis by translocating various porphyrins from the cytoplasm into the mitochondria. Here we report the cryo-electron microscopy (cryo-EM) structures of human ABCB6 with its substrates, coproporphyrin III (CPIII) and hemin, at 3.5 and 3.7 Å resolution, respectively. Metal-free porphyrin CPIII binds to ABCB6 within the central cavity, where its propionic acids form hydrogen bonds with the highly conserved Y550. The resulting structure has an overall fold similar to the inward-facing apo structure, but the two nucleotide-binding domains (NBDs) are slightly closer to each other. In contrast, when ABCB6 binds a metal-centered porphyrin hemin in complex with two glutathione molecules (1 hemin: 2 glutathione), the two NBDs end up much closer together, aligning them to bind and hydrolyze ATP more efficiently. In our structures, a glycine-rich and highly flexible "bulge" loop on TM helix 7 undergoes significant conformational changes associated with substrate binding. Our findings suggest that ABCB6 utilizes at least two distinct mechanisms to fine-tune substrate specificity and transport efficiency.

• Structural Engineering and Mechanics
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• 제85권1호
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• pp.29-53
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• 2023

High-strength shielding concrete against gamma radiation is a priority for many medical and industrial facilities. This paper aimed to investigate the gamma-ray shielding properties of high-strength hematite concrete mixed with silica fume (SF) with nanoparticles of lead dioxide (PbO₂), tungsten oxide (WO₃), and bismuth oxide (Bi₂O₃). The effect of mixing steel fibres with the aforementioned binders was also investigated. The reference mixture was prepared for high-strength concrete (HSCC) containing 100% hematite coarse and fine aggregate. Thirteen mixtures containing 5% SF and nanoparticles of PbO₂, WO₃, and Bi₂O₃ (2%, 5%, and 7% of the cement mass, respectively) were prepared. Steel fibres were added at a volume ratio of 0.28% of the volume of concrete with 5% of nanoparticles. The slump test was conducted to workability of fresh concrete Unit weight water permeability, compressive strength, splitting tensile strength, flexural strength, and modulus of elasticity tests were conducted to assess concrete's engineering properties at 28 days. Gamma-ray radiation of 137Cs emits photons with an energy of 662 keV, and that of 60Co emits two photons with energies of 1173 and 1332 keV were applied on concrete specimens to assess radiation shielding properties. Nanoparticles partially replacing cement reduced slump in workability of fresh concrete. The compressive strength of mixtures, including nanoparticles was shown to be greater, achieving 94.5 MPa for the mixture consisting of 7.5 PbO2. In contrast, the mixture (5PbO₂-F) containing steel fibres achieved the highest values for splitting tensile, flexural strength, and modulus of elasticity (11.71, 15.97, and 42,840 MPa, respectively). High-strength shielded concrete (7.5PbO2) showed the best radiation protection. It also showed the minimum concrete thickness required to prevent the transmission of radiation.

• Current Photovoltaic Research
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• 제11권3호
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• pp.67-74
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• 2023

The earth-abundant element-based Cu₂ZnSn(S,Se)₄ (CZTSSe) thin film solar cells (TFSCs) have attracted greater attention in the photovoltaic (PV) community due to their rapid development in device power conversion efficiency (PCE) >13%. In the present work, we demonstrated the fine-tuning of the bandgap in the CZTSSe TFSCs by altering the sulfur (S) to the selenium (Se) chalcogenide ratio. To achieve this, the CZTSSe absorber layers are fabricated with different S/(S+Se) ratios from 0.02 to 0.08 of their weight percentage. Further compositional, morphological, and optoelectronic properties are studied using various characterization techniques. It is observed that the change in the S/(S+Se) ratios has minimal impact on the overall Cu/(Zn+Sn) composition ratio. In contrast, the S and Se content within the CZTSSe absorber layer gets altered with a change in the S/(S+Se) ratio. It also influences the overall absorber quality and gets worse at higher S/(S+Se). Furthermore, the device performance evaluated for similar CZTSSe TFSCs showed a linear increase and decrease in the open circuit voltage (V_oc) and short circuit current density (J_sc) of the device with an increasing S/(S+Se) ratio. The external quantum efficiency (EQE) measured also exhibited a linear blue shift in absorption edge, increasing the bandgap from 1.056 eV to 1.228 eV, respectively.

• 한국의상디자인학회지
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• 제24권4호
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• pp.117-133
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• 2022

In order to reflect traditional elements in modern design, designers should be able to creatively apply elements of traditional Chinese clothing. To understand this, a deep understanding of and insights into the traditional clothing culture are required. In this study, the characteristics of traditional Chinese women's clothing from the Wei, Jin, and Northern and Southern Dynasties of China to the Qing Dynasty were analyzed by dividing them into silhouette, color, pattern, materials, and detail. The characteristics of the silhouette were classified into A, H, X, and O types, of which types A and H were the most common. As for the color characteristics, there are relatively many five cardinal colors, and for the contrast of colors complementary colors were mainly used. As the for pattern characteristics, real patterns, animal patterns, character patterns, geometric patterns, and mixed patterns were used. Four types of materials were mainly used: silk, hemp, cotton, and wool. The detail characteristics were also anlyzed by classifying them into collar, sleeve, neckband, and gusset. Based on the results of this analysis, a satisfaction survey was conducted on the design of modern Chinese women's clothing. The result of satisfaction with design elements showed that the images of vest and suit were most preferred, H and X silhouettes, and yellow and white were the most preferred. Geometric and plant patterns were preferred, as were silk and acetate materials. Based on the result of chi-square analysis of design element preferences according to the characteristics of the subject, there was no difference according to occupation, residential area, or income, and there were differences in silhouette, color, materials, and detail according to age.

• Biomolecules & Therapeutics
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• 제32권1호
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• pp.162-169
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• 2024

Particulate matter (PM) constitutes a hazardous blend of organic and inorganic particles that poses health risks. Inhalation of fine airborne PM with a diameter of ≤ 2.5 ㎛ (PM_2.5) can lead to significant lung impairments. (+)-afzelechin (AZC), a natural compound sourced from Bergenia ligulata, boasts a range of attributes, including antioxidant, antimicrobial, anticancer, and cardiovascular effects. However, knowledge about the therapeutic potential of AZC for patients with PM_2.5-induced lung injuries remains limited. Thus, in this study, we investigated the protective attributes of AZC against lung damage caused by PM_2.5 exposure. AZC was administered to the mice 30 min after intratracheal instillation of PM_2.5. Various parameters, such as changes in lung tissue wet/dry (W/D) weight ratio, total protein/total cell ratio, lymphocyte counts, levels of inflammatory cytokines in bronchoalveolar lavage fluid (BALF), vascular permeability, and histology, were evaluated in mice exposed to PM_2.5. Data demonstrated that AZC mitigated lung damage, reduced W/D weight ratio, and curbed hyperpermeability induced by PM_2.5 exposure. Furthermore, AZC effectively lowered plasma levels of inflammatory cytokines produced by PM_2.5 exposure. It reduced the total protein concentration in BALF and successfully alleviated PM_2.5-induced lymphocytosis. Additionally, AZC substantially diminished the expression levels of Toll-like receptors 4 (TLR4), MyD88, and autophagy-related proteins LC3 II and Beclin 1. In contrast, it elevated the protein phosphorylation of the mammalian target of rapamycin (mTOR). Consequently, the anti-inflammatory attribute of AZC positions it as a promising therapeutic agent for mitigating PM_2.5-induced lung injuries by modulating the TLR4-MyD88 and mTOR-autophagy pathways.