• Title/Summary/Keyword: Filament shape

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Regulated Expression of Nebulin by Transfection of Green Fluorescent Protein-Tagged Nebulin Fragments in Cultured Chicken Myoblast

  • Park, Su-Jung;Kim, Ji-Hee;Ko, Han-Suk;Kim, Chong-Rak;Kim, Han-Do;Kang, Ho-Sung
    • Biomedical Science Letters
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    • v.7 no.4
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    • pp.167-172
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    • 2001
  • Nebulin is an approximately 700 kDa filamentous protein in vertebrate skeletal muscle. It binds to the Z line and also binds side-by-side to the entire thin actin filament in a sarcomere. The correlation of nebulin size with thin filament length have led to the suggestion that nebulin acts as a molecular ruler for the length of thin filaments. The C-terminal part of human nebulin is anchored in the sarcomeric Z-disk and contains an SH3 domain. SH3 domains have been identified in an ever-increasing number of proteins important for a wide range of cellular processes, from signal transduction to cytoskeleton assembly and membrane localization. However, the exact physiological role of SH3 domains remains, in many cases, unclear. To explore the role of nebulin SH3 in the cytoskeletal rearrangement that accompanies myoblast differentiation, we transfected sense and antisense nebulin SH3 domain fused to enhanced green fluorescent protein in myoblast. Cells expressing nebulin SH3 fragment showed decrease of cell-cell adhesion, and cells transfected with antisense nebulin SH3 gene showed a rounded cell morphology and loss of cell-matrix adhesion. No alteration in cell shape and differentiation were observed in control cells expressing enhanced green fluorescent protein. Perturbation of nebulin altered the cell shape and disrupted cell adhesion in myoblast, demonstrating that nebulin can affect cytoskeleton rearrangement.

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KSR-III 복합재 가압탱크의 설계 및 제작

  • Kong, Cheol-Won;Yoon, Chong-Hoon;Jang, Young-Soon;Yi, Yeong-Moo
    • Aerospace Engineering and Technology
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    • v.2 no.2
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    • pp.124-132
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    • 2003
  • This paper described the structural design and the fabrication procedure of KSR-III composite pressure tank. The type of the composite pressure tank was COPV(Composite Overwrapped Pressure Vessel). A non-load sharing liner was made of aluminum 6061-0 and the liner provided a helium gas seal. The composite pressure tank was winded using T700 carbon/epoxy on the liner. Because the aluminum liner was thin, multiple cure cycles were applied to the filament winding technique. The multiple cure cycles prevented the liner-cylinder from losing a circular shape. A fitting force at the metallic boss was spread to the carbon fiber by a boss ring. The boss ring also prevented a local deformation at the boss part.

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Development of a 3D Printing Open-market System for Copyright Protection and Remote 3D Printing (3D프린터용 설계데이터의 저작권보호와 원격출력을 지원하는 오픈 마켓 시스템 개발)

  • Kim, Sung Gyun;Yoo, Woosik
    • Journal of Korean Institute of Industrial Engineers
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    • v.41 no.3
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    • pp.253-258
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    • 2015
  • The 3D printing is any of various processes for making a three dimensional object of almost any shape from a 3D model. Recently, a rapidly expanding hobbyist and home-use market has become established with the inauguration of the open-source RepRap and Fab@Home projects. However, this causes problems regarding copyright protection and usage of illegal 3D data. In this paper, we developed a 3D printing open-market system, which guarantees copyright protection using the remote 3D printing without direct distribution of 3D design data. Because most of the home-use 3D printers are FFF (Fused Filament Fabrication) based on NC code system, open-market system uses FFF 3D printers. Also, open-market system inspects the uploaded 3D model data, so the system can prevent distribution of illegal model data such as weapons, etc.

Ultrastructural Study on Spermatogenesis of Rockfish, Sebastes inermis (Pisces: Scorpaenidae) (볼락 (Sebastes inermis)의 정자형성과정에 관한 미세구조적 연구)

  • Lee, Jung-Sick
    • Applied Microscopy
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    • v.26 no.3
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    • pp.267-275
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    • 1996
  • The internal ultrastructural changes of germ cells and external morphology of spermatozoon during the spermatogenesis in the rockfish, Sebastes inermis were studied using transmission and scanning electron microscope. The testis is seminiferous tubule type in internal structure. Seminiferous tubule consist of many cyst which contain numerous germ cells in same developmental stage. Spermatogonium contained a large nucleus with single nucleolus in interphase. Primary spermatocyte identified by the presence of synaptonemal complex in nucleus and the contained a number of mitochondria, endoplasmic reticula and Golgi bodies in cytoplasm. The nucleoplasm of secondary spermatocyte was more concentrated than that of the previous phase. Spermatids were more condensed in nucleus and cytoplasm, and show the long-spherical shape. In the cytoplasm of spermatid mitochondria located to lower portion of the nucleus and Golgi bodies located to upper portion, but proacrosomal granule is not appeared. The spermatozoon consist of the head and tail. No acrosome could be found in the head. The cytoplasmic collar of posterior part in sperm head contained mitochondria which surrounded axial filament. The well developed axonemal lateral fins were identified in sperm flagellum, and the axial filament of the flagellum consist of nine pairs of peripheral microtubules and one pair of central microtubules.

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A Study on The Physical Properties of Sheath/Core Type Nylon/PET High Hollow Composite Yarns and its Fabrics (Sheath/Core형 나일론/PET 고중공 복합사 및 직물물성 연구)

  • Kim, Seung-Jin;Park, Kyung-Soon;Jo, Jin-Hwang
    • Textile Coloration and Finishing
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    • v.21 no.4
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    • pp.1-10
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    • 2009
  • This paper surveys the physical properties of sheath/core nylon/PET high hollow composites filaments and its fabrics according to the various elution conditions such as concentration of elution, eluted time and eluted temperature. For this purpose, sheath/core nylon/PET filament was texturized and four kinds of fabric specimens were woven with different warp and weft densities. These grey fabrics were eluted with two kinds of concentrations of NaOH (30g/l, 40g/l), three kinds of eluted temperatures $50^{\circ}C,\;60^{\circ}C,\;85^{\circ}C$) and two kinds of eluted times (60min, l20min). The elution characteristics of these specimens were investigated and discussed with different elution conditions. In addition, the mechanical properties such as extensibility, bending rigidity, shear modulus and compressional work of these specimens aceording to the elution conditions were analysed and summarized with cross-sectional shapes of eluted filaments measured by SEM.

The Effect of Cold Working and Heat Treatment on the Magnetic Properties of in-situ Formed Cu-Fe Composites (In-situ 법(法)에 의한 Cu-Fe 복합조직(複合組織)의 자기적(磁氣的) 특성(特性)에 미치는 가공(加工) 및 열처리(熱處理)의 영향(影響))

  • Shur, S.J.;Park, H.S.
    • Journal of the Korean Society for Heat Treatment
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    • v.2 no.2
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    • pp.38-45
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    • 1989
  • The Cu-Fe permanent magnet were prepared in situ process, which has economic and mass productive merits in producing multi filamentary composites. The purpose of this research was to study the effect of reduction ratio and heat treatment on magnetic property. As the reduction ratio of Cu-Fe wire increased, the filament structure became finer and interfilament distances decreased and the morphology of filament cross section became ribbon shape. As Fe content increased significantly. The coercivity and squareness of Cu-55 wt%Fe composite increased as a reduction ratio became higher, whereas they increased to maximum values at 0.09 mm ${\phi}$ for Cu-30 wt%Fe, and 0.066 mm ${\phi}$ for CU-45 wt%Fe respectively, and decreased for further reduction. The magnetic properties of Cu-Fe composites can be more enhanced by intermediate heat treatment. The best magnetic properties were obtained from Cu-55 wt%Fe composite deformed to 0.054 mm ${\phi}$ and annealed.

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The effects of the surrounding viscoelastic media on the buckling behavior of single microfilament within the cell: A mechanical model

  • Khadimallah, Mohamed A.;Safeer, Muhammad;Taj, Muhammad;Ayed, Hamdi;Hussain, Muzamal;Bouzgarrou, Souhail Mohamed;Mahmoud, S.R.;Ahmad, Manzoor;Tounsi, Abdelouahed
    • Advances in concrete construction
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    • v.10 no.2
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    • pp.141-149
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    • 2020
  • In the present study, a mechanical model is applied to account the effects of the surrounding viscoelastic media on the buckling behavior of single microfilament within the cell. The model immeasurably associates filament's bending rigidity, neighboring system elasticity, and cytosol viscosity with buckling wavelengths, buckling growth rates and buckling amplitudes of the filament. Cytoskeleton components in living cell bear large compressive force and are responsible in maintaining the cell shape. Actually these filaments are surrounded by viscoelastic media consisting of other filaments network and viscous cytosole within the cell. This surrounding, viscoelastic media affects the buckling behavior of these filaments when external force is applied on these filaments. The obtained results, indicate that the coupling of viscoelastic media with the viscous cytosol greatly affect the buckling behavior of microfilament. The buckling forces increased with the increase in the intensity of surrounding viscoelastic media.

Manufacturing Processes of Cylindrical Composite Lattice Structures using Filament Winding Method (필라멘트 와인딩 공법을 이용한 원통형 복합재 격자구조체 제작 공정)

  • Im, Jaemoon;Shin, Kwangbok;Lee, Sangwoo;Son, Johwa
    • Proceedings of the Korean Society of Propulsion Engineers Conference
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    • 2017.05a
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    • pp.835-837
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    • 2017
  • In this paper, manufacturing processes of cylindrical composite lattice structures using filament winding method was described. Cylindrical composite lattice structures were manufactured in accordance with four major steps. Silicon mold of lattice shape was installed on mandrel and then continuous fiber was wound on silicon mold. After winding process, in order to ensure the same thickness for all regions, compression process was done for its intersection parts. Finally, the composite lattice structure was demoulded after curing in oven. It was found that the manufactured cylindrical composites lattice structure had 2.4% of dimensional error compared to the design requirements.

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Studies on the Width of Rectangular Channels of Fuel Cell Bipolar Plate Using FDM 3D Printer with PLA Filament

  • Kim, Jae-Hyun;Jin, Chul-Kyu
    • Journal of the Korean Society of Industry Convergence
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    • v.24 no.6_1
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    • pp.683-691
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    • 2021
  • Bipolar plates with channel width of 0.5 mm, 0.4 mm, and 0.3 mm respectively were printed using a 3D printer. The shape of three b ipolar plates was rectangular, the channel depth was 0.5 mm, and the thickness of base was 0.5 mm. The bipolar plate with channel width of 0.5 mm had 45 channels, and their active area was 44.5 mm × 50 mm. The bipolar plate with channel width of 0.4 mm had 57 channels and its active area was 45.2 mm × 50 mm, and the bipolar plate with channel width of 0.3 mm had 75 channels and its active area was 44.7 mm × 50 mm. The bipolar plates were printed using PLA filament. The cross-sectional lengths of the bipolar plates with channel widths of 0.5 mm and 0.4 mm were identical by 96% of the designed cross-sectional length. Whereas the bipolar plate with a channel length of 0.3 mm had a large difference of 25% from the designed cross-sectional length.

Determining plasma boundary in Alvand-U tokamak

  • Yahya Sadeghi
    • Nuclear Engineering and Technology
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    • v.55 no.9
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    • pp.3485-3492
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    • 2023
  • One of the major topic of tokamak research is the determination of the magnetic profile due to magnetic coil fields and plasma current by mean of data from magnetic probes. The most practical approach is to use the current filament method, which models the plasma column with multiple current carrying filaments and the total current of these filaments is equal to the plasma current. Determining the plasma boundary in Alvand-U tokamak is the main purpose of this paper. In order to determine the magnetic field profile and plasma boundary, information concerning the magnetic coils, their position, and current is required in the computing code. Then, the plasma shape is determined and finally the plasma boundary is extracted by the code. In the conducted research, we discuss how to determine the plasma boundary and the performance of the computing code for extraction of the plasma boundary. The developed algorithm shows to be effective by running it in the regular pc machine with characteristics of Intel (R) core (TM) i3-10100 CPU @3.60 GHz and 8.00 GB of RAM. Finally, we present results of a test run for computing code using a typical experimental pulse.