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An investigation of Panax ginseng Meyer growth promotion and the biocontrol potential of antagonistic bacteria against ginseng black spot

  • Sun, Zhuo;Yang, Limin;Zhang, Lianxue;Han, Mei
    • Journal of Ginseng Research
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    • v.42 no.3
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    • pp.304-311
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    • 2018
  • Background: Ginseng black spot disease resulting from Alternaria panax Whuetz is a common soil-borne disease, with an annual incidence rate higher than 20-30%. In this study, the bacterial strains with good antagonistic effect against A. panax are screened. Methods: A total of 285 bacterial strains isolated from ginseng rhizosphere soils were screened using the Kirby-Bauer disk diffusion method and the Oxford cup plate assay. We analyzed the antifungal spectrum of SZ-22 by confronting incubation. To evaluate the efficacy of biocontrol against ginseng black spot and for growth promotion by SZ-22, we performed pot experiments in a plastic greenhouse. Taxonomic position of SZ-22 was identified using morphology, physiological, and biochemical characteristics, 16S ribosomal DNA, and gyrB sequences. Results: SZ-22 (which was identified as Brevundimonas terrae) showed the strongest inhibition rate against A. panax, which showed 83.70% inhibition, and it also provided broad-spectrum antifungal effects. The inhibition efficacies of the SZ-22 bacterial suspension against ginseng black spot reached 82.47% inhibition, which is significantly higher than that of the 25% suspension concentrate azoxystrobin fungicide treatment (p < 0.05). Moreover, the SZ-22 bacterial suspension also caused ginseng plant growth promotion as well as root enhancement. Conclusion: Although the results of the outdoor pot-culture method were influenced by the pathogen inoculum density, the cropping history of the field site, and the weather conditions, B. terrae SZ-22 controlled ginseng black spot and promoted ginseng growth successfully. This study provides resource for the biocontrol of ginseng black spot.

REMOVAL OF FOREIGN BODY IN THE LATERAL PHARYNGEAL SPACE VIA TRANSTONSILLAR APPROACH (편도와 접근을 통한 측방인두간극 이물제거의 치험 증례)

  • Kim, Soung-Min;Kim, Han-Seok;Kim, Ji-Hyuck;Kwon, Kwang-Jun;Park, Young-Wook
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.33 no.5
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    • pp.567-571
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    • 2007
  • Lateral pharyngeal space is one of potential fascial planes of head and neck, that may become involved by various pathological processes, such as infection, inflammation and neoplasm. The calcified stylohyoid ligament with styloid process is also located in this space, so this space is more acquainted with Eagle's syndrome in oral and maxillofacial field. During the mandibular transbuccal fixation procedures of 29-year old female patient who had right condylar neck and left parasymphysis fracture, we had lost one 10.0 mm miniscrew. After confirming the location of the lost miniscrew from different angled plain skull radiographies, we tried to find it in the lateral pharyngeal space via transtonsillar approach at the time of plate removal operation. This case report is aimed to share our valuable experience of the effective approach way to the lateral pharyngeal space, which has many advantages, such as short operative time, minimal bleeding, fast post-operative recovery, and less morbidity. The related literature is also reviewed.

The status of Cleft Lip and Palate in North Korea; Analysis of North Korean textbooks (교과서 분석을 통해 본 북한의 구순$\cdot$구개열 현황)

  • Huh Jin-Young;Kim Tae-Yeon;Kim Bum-Su;Yi Choong-Kook
    • Korean Journal of Cleft Lip And Palate
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    • v.4 no.2
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    • pp.1-8
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    • 2001
  • The dissimilarities between South and North Korea have persisted in spite of the reconciliation campaign by both countries. The situation of the cleft lip & palate of North Korea was very unclear until now. The purpose of this study is to understand all the current facts of cleft lip & palate in North Korea so that we can find ways of helping North Korea in this field of medicine. The present data and analysis are extracted from North Korean textbooks. The results are as follow. 1. In North Korea, patients with CLP are treated by oral surgeons or maxillofacial surgeons. The detailed contents about the CLP are well described in the North Korean textbooks for the dental students. 2. The terminology of CLP in North Korea has changed from time to time, but the present terminology not being so different from South Korean counterpart. So there will be no particular problems in mutual communication. 3. The main classification for CLP in North Korea originated from Kernahan & Stark's classification as is with South Korea. 4. The incidence of CLP is 1 : 1,000-1,200 in North Korea, which is lower than that of South Korea. There is, however, some difference between the North and South Korean CLP in detailed statistics. 5. We found the North Korean physicians have shown much interest in pursuing the etiology and the prevention of CLP. 6. The timing of CLP operations varied a lot in North Korea. There was recommendation by few for the operation in much late age than in South Korea. 7. The classical operation techniques of cleft lip have changed. For unilateral cleft lip Tennison-Randall method was replaced by Millard I method: and for bilateral cleft lip LeMesurier method was replaced by Veau III and Tennison methods. But for cleft palate Pushback palatoplasty has been utilized consistently.

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Unidirectonal single-mode operation of a Nd:YAG laser by using a planar semimonolithic ring cavity (평면 반일체 고리형 공진기를 이용한 Nd:YAG 레이저의 단방향 단일 모드 발진)

  • 박종락;이해웅;윤태현;정명세
    • Korean Journal of Optics and Photonics
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    • v.10 no.4
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    • pp.311-317
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    • 1999
  • Unidirectional single-mode operation of a diode-pumped Nd:YAG laser with a planar semimonolithic ring cavity has been demostrated at 1064 nm. The semimonolithic cavity consists of a laser active medium placed in a magnetic field, a crystal quartz plate, and an output coupling mirror. They form an optical diode by acting each part as a Faraday rotator, a reciprocal polarization rotator and a partial polarizer, respectively. An eigenpolarization theory for the cavity configuration was presented and losses for the eigenmodes were calculated. A pump-limited single-mode output power of 155 mW and a slope efficiency of 17% were obtained when the laser was pumped by a 1.2 W, 809 nm diode-laser. A laser linewidth of less than 100 kHz is inferred from a beat note frequency spectrum between two identical laser systems and continous single-mode tuning range was more than 2 GHz.

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Effects of Herbal-Acupuncture with Evodiae Fructus at KI10 on Osteoporosis in Ovariectomized Mice (음곡(陰谷) 오수유(吳茱萸) 약침(藥鍼)이 난소적출(卵巢摘出)생쥐의 골다공증(骨多孔症)에 미치는 영향(影響))

  • Choi, Sung-Hoon;Lee, Byung-Ryul;Yang, Gi-Young;Kim, Jae-Kue;Seo, Young-Suk;Yim, Yun-Kyoung
    • Korean Journal of Acupuncture
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    • v.27 no.2
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    • pp.217-242
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    • 2010
  • Objective : This study is aimed to evaluate the effects of Evodiae Fructus herbal-acupuncture (EF-HA) at KI10 on osteoporosis induced by ovariectomy in mice. Method : Mice underwent bilateral ovariectomy. After recovering, the ovariectomized (OVX) mice were treated by needle prick, saline injection, herbal acupuncture with Evodiae Fructus (EF-HA) at KI10 for 8 weeks. Result : 1. EF-HA at KI10 significantly inhibited the overgrowth of tibia in ovariectomized mice. 2. NP at KI10 significantly restored the tibial BMD (bone mineral density) in ovariectomized mice. 3. EF-HA at KI10 significantly restored the phosphorus and creatinine levels in ovariectomized mice serum. 4. EF-HA at KI10 significantly restored the tibial Ca and P levels in ovariectomized mice. 5. EF-HA at KI10 significantly reduced the tibial osteoclast-like cells in ovariectomized mice. 6. EF-HA at KI10 significantly inhibited the overgrowth of tibial GPL (growth plate length) in ovariectomized mice. Conclusion : EF-HA at KI10 has protective and therapeutic effect for osteoporosis in ovariectomized mice. Thus, it is suggested that EF-HA can be an useful therapeutics in clinical field after further researches.

Studies of plant Tumor induction (III) - Antimicrobial action of some bacteriacidal agents to obtain Bacteria-Free Tumor tissue (식물의 암종유발에 관한 연구 (제 3보) - Bacteria-free 암종조직의 획득에 있어서 항생제의 효과)

  • 이민재;홍순우;최영길
    • Korean Journal of Microbiology
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    • v.5 no.2
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    • pp.97-101
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    • 1967
  • Up to the present time, there are only three methods by which we can obtain bacteria free crown gall tissue. According to some papers related to this field, the first method is based on the works of Braun(53') who maintained infected plants at 46-47'c for several days. But the method has a problem that very few plants can tolerate this temperature. The second method is based on the well known observation that old tumors appear to be bacteria free at least 1 or 2% of the explants. Also this method is known to us as laborious and time consuming. The third method is the one we were using that was attempting to kill the bacteria with bacteriacidal agent such as Antibiotics. In fact., it is reported that almost complete control of crown gall of tomato was obtained by Blanchard('51) when plants were grown in a nutrient containing Aureomycin(20${\mu}g$/ml) following needle puncture with the gall bacteria. We have been engaged in making the experiment by applying solution of Penicillin, Streptomycin and of Chloramphenicol(Succinate free) to find the strong bacteriacidal agent through the method of disc plate, and to confirm the effect of antimicrobial action through the method of plant tissue culture system without possible injury to the host plant. The result of this report is the fact the strongest bacteriacidal agent among the above three Antibiotics was Chloramphenicol(Succinate free 1000 p.p.m). and that there happened no injury to the tissue cultures in a White's 10X media containing 1000 p.p.m. of Chloramphenicol.

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Fungs flora of paddy fields in Korea. - III. Ascomycetes - (한국 논 토양중의 균류에 관한 연구 - III . 자낭균류 -)

  • Kyung Hee MIN;Tadayoshi ITO;Tatsuo YOKOYAMA
    • Korean Journal of Microbiology
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    • v.20 no.2
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    • pp.80-88
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    • 1982
  • Soil microfungi of the paddy fields in Korea were isolated by the dilution plate method from soil samples of two selected sites. It was concluded that 14 species among 30 species identified were undescribed fungi in Korea. Among them, 7 species of Ascomycetous fungi were described in this paper as new to Korea. Species of the genus Talaromyces were found to be dominant in paddy field soils and they consisted of Talaromyces flavus var. flavus, T. panasenroi, T.stipitalus and T.trachyspermus. Special attention was paid on the predominant occurrance of Westerdyrella multispora which produced globose to subglobose pseudothecia containing 32 spored asci with multiseptate, cylindrical ascospores. A cellulose decomposing ascomycete, Chaetomium globosum, was also found which produce black, ostiolate perithecia furnished with numerous, wavy to undulate terminal hairs. They contain evansecent, clubshaped, 8 spored asci with lemon-shaped, olive brown ascospores. Another ascomycte, Emericellopsis terricola with Acremonium anamorph, waas isolated from two sites.

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Late Neogene and Quaternary Vertical Motions in the Otway Coast, Southeast Australia (II): Epeirogenic Uplift Driven by Lithospheric Flexural Deformation (호주 남동부 Otway 해안의 후기 신제3기 및 제4기 융기 운동(II): 암석권 휨 현상에 의한 대륙 지각의 융기)

  • Shin, Jaeryul
    • Journal of the Korean earth science society
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    • v.33 no.6
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    • pp.534-543
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    • 2012
  • The relationship between tectonic uplift and geophysical analysis of gravity anomalies and the in-situ stress fields in the Otway Ranges, SE Australia is addressed in this study to understand the nature and possible mechanism for the neotectonic movements. The uplift axis of the ranges is coincident with the regional Bouguer gravity highs whereas thick Tertiary sedimentary successions are highly correlated with the gravity lows along the basin rift geometry. This result suggests that the gravity highs are separated by the thick Tertiary sedimentary successions. Regional structural trends associated with faults and foldings of the deformed surfaces are consistent with the prevailing NW-SE $S_{Hmax}$ trend in this part of the continent. The anomalously positive correlation between topography and Bouguer gravity fields suggests possibly a lithospheric flexural deformation mode at a long wavelength (order of $10^2$ kms) in the region. It also suggests that the reactivation of pre-existing lithospheric structures driven by plate boundary forces plays a key role in this mode.

Microwave Propagation in the Plasma for 28 GHz Superconducting ECRIS (28 GHz 초전도 ECRIS 플라즈마에서의 마이크로파 전파)

  • Wang, S.J.;Won, M.S.;Lee, B.S.;Kim, S.H.;Kwak, J.G.;Jeong, S.H.;Kim, S.K.;An, C.Y.
    • Journal of the Korean Vacuum Society
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    • v.19 no.6
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    • pp.467-474
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    • 2010
  • Packet propagation and absorption for the 28 GHz superconducting ECRIS under developing by KBSI Pusan center is analyzed with limited parameter range. The microwave power generated by 28 GHz gyrotron is axially injected to the plasma cavity through waveguide system. According to the analytical ray tracing calculation, the wave packet launched quasi-longitudinally at a high magnetic field side changes its direction from outward to inward as it is approaching resonance layer. Therefore, initially diverging wave does not likely hit a conducting surface before absorbing by electron cyclotron resonance. Also, absorption by plasma with moderate electron density is so strong that reflection by an extraction plate may not be expected.

A Study on the Mitigation of Welding Distortion of a Precision Component for Automobile Transmission (자동차 변속기용 정밀 부품의 용접변형 감소화에 관한 연구)

  • Chung, Hoi-Yoon;Kim, Jae-Woong;Yun, Seok-Chul
    • Journal of Welding and Joining
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    • v.30 no.4
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    • pp.31-37
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    • 2012
  • In recent years, a demand for precision-welding is increasing in wide industrial fields for getting a high quality welded structures. Although laser welding is commonly used for precision-welding, gas tungsten arc (GTA) welding is also attempted as a precision-welding due to the cost benefit. However, welding heat causes an uneven temperature distribution leading to welding deformation. Since it causes geometric errors and degrades product quality, welding distortion recently rises as an important issue in the field of automobile parts. To control welding deformation, it is needed to design in shapes that can maximize stiffness against deformation during welding; control the welding sequence; minimize heat input; and weld allowing reverse deformation; etc. Thus it is necessary to find the one, among such approaches, that can minimize the deformation range by mathematical analysis and understand how effective it would be when it is actually used in industrial fields. This study performs analyses by numerical calculations and experiments for the De-Tent Lever, one of transmission part that requires precision the most among automobile parts, as the subject of experiment. Decrease in welding deformation is required for this part, since there is currently a trouble in guaranteeing precision due to angular deformation by welding between boss and plate. Finally the ways to minimize welding deformation has been suggested in this study through analyses on it.