• Research in Plant Disease
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• v.18 no.4
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• pp.331-337
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• 2012

Citrus melanose is one of important disease in citrus cultivation, reducing quality of citrus fruits and resulting in economic loss. Like other diseases in citrus, melanose was mainly protected by chemical fungicide in the field. Recently, alternative method of disease control is highly required due to the side effect of the chemicals. In this study four rhizobacterial strains TRH423-3, MRL408-3, THJ609-3, and TRH415-2 are selected by dual-culture testing its antifungal activity against Diaporthe citri causing citrus melanose. To investigate the protection efficacy of the selected rhizobacterial strains to citrus melanose, the bacteria were pre-treated on citrus leaves following inoculation with melanose pathogen. Pre-treatment with all selected rhizobacterial strains showed disease suppression in which the levels of protection rates were different by the rhizobacterial strains. Additional treatment with the rhizobacterial strains after the pathogen inoculation enhanced protection rates in all cases. The strain MRL408-3 and TRH423-3 were identified as Burkholderia gladioli, TRH415-2 as Pseudomons fluorescens and THJ609-3 as Pseudomonas pudia as a result of analyzing the internal transcript spaces of the rhizobacterial strains rDNA. The selected rhizobacterial strains may be valuable as biological control agents in the environment-friendly citrus farm in which chemical application is limited.

• Korean journal of applied entomology
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• v.55 no.2
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• pp.81-89
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• 2016

Double-stranded RNA (dsRNA) has been applied to control insect pests by its suppressive activity against specific target genes. Integrin is a heterodimer (${\alpha}$ and ${\beta}$) transmembrane protein and plays a critical role in cell-to-cell or cell-to-extracellular matrix interactions in eukaryotes. Suppression of ${\beta}$ subunit integrin gene expression by its specific dsRNA (= dsINT) induces significant mortality against target insects. Furthermore, a recombinant bacterium expressing dsINT is potent to kill target insects. However, it is necessary to develop a formulation technique of the dsRNA-expressing bacteria to apply the bacterial insecticide against field populations. This study formulated the recombinant bacteria by freeze-drying and tested its control efficacy against target insects. The formulation maintained significant insecticidal activity against last instar larvae of Spodoptera exigua. While a commercial Bacillus thuringiensis (Bt) insecticide exhibited only about 60% insecticidal activity against S. exigua last instar, an addition of the dsINT-expressing bacterial formulation significantly enhanced the Bt insecticidal activity. The dsINT-expressing bacterial formulation exhibited relative selectivity to target insects depending on sequence similarity. These results indicate that a freeze-dried form of dsRNA-expressing bacteria keeps its insecticidal activity.

• The Journal of Korean Association of Computer Education
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• v.20 no.6
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• pp.47-59
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• 2017

The Goal of SW education is to cultivate creative and convergent human resources with computational thinking ability. The content and methods of SW education are diverse, and it is difficult for the students to properly evaluate what they have learned. In order to appropriately evaluate the learning contents, SW education evaluations should be able to easily evaluate the core content of the SW education. The purpose of this study is to provide a systematic framework that can be used to develop evaluation factors to evaluate the effectiveness of SW education in elementary and secondary schools. We conducted a literature review, a field suitability review through FGI, an expert consultation, and a Delphi survey. As a result, the metrics of the cognitive domain were developed with 17 keywords in three categories: Computational Materials & Outputs (CMO), Computational Concepts (CC), and Computational Practices (CP). Also, metrics of the affective domain were developed with 13 sub-areas in four categories: value, attitude, computational thinking efficacy, and interest. The SW education evaluation factors developed in this study can be used as a framework to develop the evaluation contents in accordance with the contents of education.

• Korean Journal of Agricultural Science
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• v.12 no.2
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• pp.183-190
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• 1985

To determine anti-lodging effect of paclobutrazol and its influence on yield of rice, paclobutrazol was applied at 12 and 18g ai/10a to cv. Nakdongbyeo and cv. Seomjinbyeo 20, 15, and 10 days before heading under 12 and 20kg/10a nitrogen conditions. 1. Paclobutrazol reduced internode length of rice, especially internode N3. Higher suppression of stem of both cultivars and reduction of ear length in cv. Nakdongbyeo were observed when paclohutrazol was applied at 18g ai/10a 15 and 20 days before heading. 2. Paclobutrazol increased culm cross-section area, culm diameter, linear density and stem flexure resistance which affected anti-lodging efficacy. Although actual lodging was not occurred in field, less stem inclination was observed at paclobutrazol treated plots. 3. There were significant correlation between stem flexure resistance and culm cross-section area, culm diameter and linear density, and negative correlation between straw inclination angle and culm cross-section area and culm diameter. 4. Slight yield reduction was observed when paclobutrazol was applied at 18g ai/10a 20 days before heading. However, when paclobutrazol was applied at 12g ai/10a 10 and 15 days before heading, rice yield was not decreased compared to untreated control.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.29 no.3
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• pp.139-146
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• 2017

In order to understand the efficacy of submerged breakwater constructed for the beach protection, laboratory experiments were carried out by observing the characteristics of flow around a single shore-parallel submerged breakwater. The velocity field near the shoreline was measured by utilizing the LSPIV (Large-Scale Particle Image Velocimetry) technique, and mean surface and wave height distributions were observed around the submerged breakwater, according to various combinations of incident waves and submerged breakwaters. In this experiment, it was found that the mean flow pattern behind the submerged breakwater was determined by the balance among the gradients of mean water surface and excess wave-momentum flux (i.e., radiation stress tensors) which interact with the wave-induced current developed by the gradients on the rear and the side of the submerged breakwater. The divergent and convergent flow patterns behind the submerged breakwater (i.e., accretion and erosion response) of the numerical study of Ranasinghe et al.(2010) were observed in the measured velocity distributions, and their empirical formula mostly agreed with the experimental results. However, for some cases in this experiment, it was difficult to say that the flow pattern was one of them and was agreed with the empirical formula.

• Korean Journal of Veterinary Service
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• v.30 no.4
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• pp.497-503
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• 2007

The humoral immune response of chicken vaccinated with fowl and pigeon pox virus vaccines was determined with the protective potentiality of the two vaccines in field condition of Bangladesh. Different aged Fayoumi chicks were subjected for the study. To assess the relationship with better immune response among experimental groups, the average percentage of 'take reaction' was examined and recorded to 97.77% in group A, 93.33% in group B and 100.0% in group C. The level of immune status induced by different vaccinated group was measured by passive hemagglutination (PHA) microplate test method. The mean PHA titer levels after primary vaccination were $33.06{\pm}14.13$ in group A, $32.0{\pm}14.81$ in group B, and $33.0{\pm}13.66$ in group C. Following booster vaccination, the mean PHA titer levels in prior of challenge were increased to $55.46{\pm}14.64$ in groups A and C, and $46.93{\pm}16.52$ in group B. The recorded PHA titer levels of each group at two weeks after challenge were significantly increased to $106.66{\pm}31.22$, $93.86{\pm}33.04$ and $110.93{\pm}29.29$, respectively. The PHA titer levels after vaccination and challenge were significantly increased compared to pre-vaccination titer levels (P<0.01). Although the PHA titer levels among three groups administrated different vaccine combinations in prior of challenge were significantly varied (P<0.01), it was observed that all of the vaccinated chicks were highly protected against challenge infection.

• Korean journal of applied entomology
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• v.46 no.1 s.145
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• pp.159-164
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• 2007

Pea weevil was easily observed in the flower and pod of garden pea, but not observed in soybean at various locations in Yeongnam district through 2001 to 2003. Number of pea weevil observed in pea flower was the highest at Milyang (20), followed by Yangsan (15), Sacheon (14) and Changnyong (13), and was the lowest at Pohang (3). On the other hand, number of pea weevil observed in pea pod was the highest at Tongyeong (192), followed by Changnyong (171), Sacheon (157) and Changwon (138), and was the lowest at Pohang (12) which showed simila. tendency with the result of pea flower. Number of pea weevil occurrence observed in pea pod after one and two times applications of Insecticides in pea field were different at harvest day of 30th May while were not significantly different at harvest day of 5th June. Likewise, number of pea pod damage after one and two times applications of insecticides were different at harvest day of 10th May while was not different at harvest day of 5th June. Thus, control efficacies of insecticides according to application times against pea weevil showed very high with above 95% at harvest day of 6th June while showed variable control efficacies at harvest of 30th May.

• KSBB Journal
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• v.25 no.4
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• pp.303-310
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• 2010

Probiotics beneficially affect the health of the host via various mechanisms in the intestine. Recent developments in probiotic products have mainly been made to maximize probiotic effects in human. In this regard, probiotic products containing doubly coated or encapsulated cells, multi-species probiotics, or high viable cell number (1010 viable cells/gram or more) have been developed and are already available in the market. Until now, the majority of probiotics contain live cells but little attention has been paid to other alternative products such as heat-killed cell or bacteriocin-containing ones, which could have broad applications due to advantages over live cell-based probiotics, such as safety and stability. In addition, genetically engineered lactic acid bacteria could be of great importance in the field of alimentary health if they are carefully designed for biological safety. Although a number of probiotics are marketed by claiming health benefits, regulations for health claims will be more stringent. Therefore sufficient scientific and clinical evidences supporting the safety and efficacy of the potential probiotic strain will be required by the regulatory authority for a health claim, which thus may have a huge impact on the future probiotic market.

• Korean Journal of Weed Science
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• v.31 no.2
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• pp.183-191
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• 2011

Weeding effect was investigated based on the leaf stages to several different herbicide treatments for an integrated weed management of herbicide resistant Echinochloa oryzoides to ACCase and ALS inhibitors in a rice field. Efficacy of soil-applied herbicide treatments before resistant E. oryzoides occurred was very effective. Pentaxazon 5% SC showed over 98% of weeding effect although E. oryzoides were emerged 31 days after the treatment. Until the leaf stage of 2.5, five herbicides, azimsulfuron carfenstole 1.05% GR, bensulfuron-methyl benzobicyclone mefenacet 24.52% SC, bensulfuron-methyl fentrazamide 7% SC, bensulfuron-methyl mefenacet oxadiargyl 21.6% SC and mefenacet pyrazosulfuron-ethyl 3.57% GR showed perfect weeding effect. Benzobicyclone mefenacet penoxulam 21.5% SC and mefenacet pyrazosulfuron-ethyl 3.57% GR were effective at the leaf stage of 3.0. It is very important to select the right herbicides for timing and their systematic application for controlling of E. oryzoides resistant to ACCase- and ALS-inhibitors.

• The Korean Society of Law and Medicine
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• v.11 no.2
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• pp.113-145
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• 2010

Nowadays, it is surely the quack which stands as one of the most controversial, problematic. the quack has been a consistent target of contested public protection strategies in the past few centuries in many countries. Recently, complementary and alternative medicine (CAM) is increasingly utilized and accepted by patients and providers throughout the health care system in the world, most accounts attribute this growing acceptability to the shortcomings of conventional medicine, the appeal of CAM's core beliefs, and the growing body of research indicating that CAM actually works. However, the governments of western countries have called for measures to ensure that the public are protected from incompetent and dangerous practitioners. Common to these controversies has been a suggestion to ban, exclude or limit the medical practice of those deemed to be damaging rather than improving the health of individuals as a measure of public protection. This article describes the experiences of western counties' health care system which is moving in a more pluralistic direction. By examining the ways in which regulatory efforts in the countries have come to address what is invariably described as a growing interest in CAM, this study show how the problem of CAM/quackery today is increasingly located in an ethical field of practitioner competency, qualifications, conduct, responsibility and personal professional development, regardless of the form of therapy in question. Many countries developed a series of measures and strategies to contain the acceptance of CAM groups, such as insisting on scientific evidence of safety and efficacy, resisting integration of CAM with conventional medicine and opposing government support for research and education. In a sense, those countries' movements serve to protect not only patients, but the dominant position of medicine and its allied professions, and to maintain existing jurisdictional boundaries within the healthcare system. The popular support for CAM will require that health professional stakeholders continue to address the challenges this poses, and at the same time protect their position at healthcare system. To cope with the quack, professional body, public sector and health authorities should consider the safety of consumers of healthcare and responding to the demands of the community for CAM therapies as well as the claims of the established healthcare professions. Finally, some implications for future health care were suggested.