Kim, Ja-Hyun;Seo, Jin-Won;Na, Young-Eun;An, Kwang-Guk
Korean Journal of Ecology and Environment
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v.40
no.1
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pp.130-142
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2007
This study was to examine impacts of turbid water on fish community in the downstream of Yongdam Dam during the period from June to October 2006. For the research, we selected six sampling sites in the field: two sites were controls with no influences of turbid water from the dam and other remaining four sites were the stations for an assessment of potential turbid effects. We evaluated integrative health conditions throughout applications of various models such as necropsy-based fish health assessment model (FHA), Index of Biological Integrity (IBI) using fish assemblages, and Qualitative Habitat Evaluation Index (QHEI). Laboratory tests on fish exposure under 400 NTU were performed to find out impact of turbid water using scanning electron microscope (SEM). Results showed that fine solid particles were clogging in the gill in the treatments, while particles were not found in the control. This results indicate that when inorganic turbidity increases abruptedly, fish may have a mechanical abrasion or respiratory blocking. The stream health condition, based on the IBI values, ranged between 38 and 48 (average: 42), indicating a "excellent" or "good" condition after the criteria of US EPA (1993). In the mean time, physical habitat condition, based on the QHEI, ranged 97 to 187 (average 154), indicating a "suboptimal condition". These biological outcomes were compared with chemical dataset: IBI values were more correlated (r=0.526, p<0.05, n=18) with QHEI rather than chemical water quality, based on turbidity (r=0.260, p>0.05, n=18). Analysis of the FHA showed that the individual health indicated "excellent condition", while QHEI showed no habitat disturbances (especially bottom substrate and embeddeness), food-web, and spawning place. Consequently, we concluded that the ecological health in downstream of Yongdam Dam was not impacted by the turbid water.
This study was conducted to investigate the effects of embryo development by fusion condition on the nuclear transfer with brindle coated cow's ear cells. Ear cells were transferred into an enucleated oocyte and fused with cytoplasm in the fusion condition with 1.9kv/cm, 2.0kv/cm, 2.1kv/cm each 10 and 20ug duration Nuclear transfer embryo were activeted with a combination of 5ug/ml and 1.9mM 6-DMAP (4min, 4h). Fusion rate was 51∼68% range among fusion condition (1.9, 2.0, 2.1kv/cm; 10, 20us). But, cytoplasm lysis rate was increased by higher electric condition (0∼51.8% range). Each parameter's cleavage and blastocyst formation rate were 1.9kv/cm for 10us (75.8 and 19.5%), 20us (69.8 and 48.6%), 2.0kv/cm for 10us (76.9 and 20.0%), 20 us (68.5 and 40.9%), 2.1kv/cm for 10us (70.5 and 44.2%), 20 us (68.5 and 27.0%). We compared the effectiveness of cloning for between brindle coated cow's ear cells and Hanwoo fetal fibroblast cells. There was no significant differences in the fusion rate and developmental rate to the blastocyst stage. After transfer of blastocysts derived from nuclear transfer embryos, pregnancy rates of the Hanwoo fetal fibroblast cells and brindle coated cow's ear cells were checked pregnant on day 60 as assessed by ultrasonography, 40% (2/5) and 15.8% (3/19), respectively. This studies conclude that brindle coated cow's ear cells have the developmental potentiality to term by nuclear transfer. These results demonstrate that the increased the field strength was to be profitable for development of blastocyst or reduce of cytoplasm's damage than increasing the pulse duration.
Despite to the reality that the outbreaks from flood poisoning in Korea have been continuously increasing in the last two decades, it was very much neglected even in the public health field in Korea. Food poisoning outbreaks resulted in many cases not only in the damage of health but also in the death of many lives. However, this problem can be effectively solved by effects through health education activities, but not solely by the legal measures. This study was carried out to provide information that can be used in planning health education programs and proposing new rules to prevent any possible outbreaks from flood poisoning. The main problems contributing to flood poisoning outbreaks in an institutional setting or school catering and at home were reviewed and analyzed through the epidemiological investigations and articles related to flood poisoning in the last a decade (1991 ∼ 2000). Accordingly, the data presented in this study are sufficient to show and prove the significant trends in food poisoning accidents in Korea. The major findings investigated in this study are as follows. The frequency of food poisoning accidents as well as the number of victims have continuously increased in Korea. The number of victims per food poisoning accident is also increased from 20 persons in 1990 to 69.8 persons in 2000. Therefore, we should realize that the group poisoning outbreaks occur more frequently and the size of group poisoning accidents is getting larger. Among four seasons, the food poisoning accidents occurred more frequently in the summer (May ∼September) until 1997, However, after 1997, the food poisoning accidents occurred evenly in three seasons except the winter. The most important bacteria that cause food poisoning in Korea were Salmonella spp., Vibrio spp. and Staphylococcus aureus. They occupied 85∼90% of the rates of accidents and the number of victims.
Journal of The Korean Society of Grassland and Forage Science
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v.5
no.2
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pp.127-135
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1985
The effects of morphological development and environmental temperature on synthesis and accumulation behavior of cell-wall constituents were studied in maize cv. Blizzard and sorghum cv. Sioux and Pioneer 931 at Muenchen Technical University from 1979 to 1981. Various growth stages of maize and sorghum plants were grown on field and phytotron at 4 temperature regimes of 30/25, 25/20, 28/18 and 18/8 degree C and mid-summer sunlight over 13-hour days. The results are summarized as follow: 1. Cell-wall constituents in sorghum and maize plants were shown to have a great synthesis rates at early growth stage from growing point differentiation to final leaf visible. The highest concentration of cell wall contents were found at heading stage with 52-54% and 64-68% of neutral detergence fiber, and 30% and 45% of acid detergence fiber foe maize and sorghum, respectively. 2. The structural carbohydrates, cellulose and hemicellulose, were found as a main components of cell-wall constituents. Cellulose were mainly accumulated in stalks, while hemicellulose were an important cell wall components in leaves and panicle. 3. Synthesis rates of cell-wall constituents and non-strnctural carbohydrates were associated with increasing of temperature. Reserved carbohydrates such as fructosan, mono - and dissaccharose in plant were, however, declined when the temperature exceeded 30 deg C, during the accumulation of cellulose, hemicellulose and lignin were increased continuously. 4. Cell-wall constituents lowered digestibility and net energy accumulation in sorghum and maize plants. In a in vitro and in vivo trial, it was found a negative correlation between digestion dry matter and cell wall constituents, especially cellulose and lignin.
This experiment was conducted in the field to investigate the effects of mulched condition on nutrient's mineralization and its mobility as affected by environmental changes (soil moisture and soil temperature) in the plow layer. 1. Nitrogen mineralization was faster owing to the prevention of evaporation and the increase of soil temperature in the poly ethylene mulched plot. As a result, nitrate form of nitrogen was much earlier available for plant in mulched plot than in non mulched plot. 2. Available $P_2O_5$ content in the plow layer was kept highest in the fertilized zone (10-20 cm depth) regardless of difference between mulched and non mulched condition, and was nearly constant without showing difference between planting and non planting of tobacco due to the lower availability of phosphorus by tobacco. 3. Potassium applied in the plow layer was remained a large quantity until the latter growth stage resulting in the lower recovery of potassium by tobacco. 4. Mineralized nutrients such as $NH_4-N$, $NO_3-N$, and K in the plow layer of mulched plot were transported from the fertilized layer (10-20 cm depth) and accumulated to the surface layer (0-10 cm depth) as the growing time goes by, but those in non mulched plot were distributed to the deeper layer. 5. Mobility of available nutrients in the plow layer was If order as $NO_3-N$$$\geq_-$$$NH_4-N$ > K > available $P_2O_5$. 6. Leaching degree of mineralized nutrients in the mulched plot was much lower than in the non mulched plot.
A field experiment was conducted at Cheju from early March 1998 to early March 1999 to evaluate the effects of foliar applied urea on leaf N content and N recovery in satsuma mandarins (Citrus unshiu Marc.). Seven years old 'Okitsu Wase' trees received foliar spray of urea (22 or 43 g N $tree^{-1}yr^{-1}$) or soil application of urea (86 g N $tree^{-1}yr^{-1}$). 56% of N was applied in spring, 11% in summer and 33% in fall. There were seven trees per N treatment and two trees per N treatment received $^{15}N$-labeled urea in spring and summer to determine N recovery. There were no differences between the treatments for fruit yield and its quality. Nitrogen content of spring flush leaf blades up to early September was greater for trees received foliar spray comparing with soil application but was not greatly affected by any treatment after mid-November. The recovery of fertilizer N in various parts of trees receiving foliar spray of 22 g N $tree^{-1}yr^{-1}$ was greatest, followed by receiving foliar spray of 43 g N and soil application of 86 g N. The recovery of fertilizer N in tree was 29.2 and 17.7% for foliar spray of 22 and 43 g N $tree^{-1}yr^{-1}$, respectively and 8.0% for soil application of 86 g N $tree^{-1}yr^{-1}$. The recovery of fertilizer N in the upper 40 cm of soil was 50.3, 45.6, and 51.8% for foliar spray of 22 and 43 g N $tree^{-1}yr^{-1}$, and soil application of 86 g N $tree^{-1}yr^{-1}$ respectively. The total (tree, fallen leaves, winter weeds, and soil) recovery of fertilizer N was 81.8, 65.1, and 60.6% for foliar spray of 22 and 43 g N $tree^{-1}yr^{-1}$, and soil application of 86 g N $tree^{-1}yr^{-1}$, respectively.
Kim, Jong-Gu;Lee, Kyeong-Bo;Lee, Sang-Bok;Lee, Deog-Bae;Kim, Seong-Jo
Korean Journal of Soil Science and Fertilizer
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v.32
no.3
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pp.239-253
/
1999
The objective of this study was to determine the effects of various kinds of composts on the change of soil chemical properties in upland soils. Field experiments were conducted in the loam and sandy loam soils. Various kinds of composts such as poultry manure compost(PMC), cow manure compost(CMC), human excrement sludge(HES), and food industrial sludge compost(FISC) were applied annually at rates of 0, 40, and $80Mg\;ha^{-1}$ to soils grown with soybean and maize plants for 4 years during 1994 to 1997. The results of this study were as follows : The continuous application of human excrement sludge decreased soil pH up to 4.4~5.0, while other compost treatments increased soil pH compared with control plot. The EC increased initially and showed their maximum values at 20days after compost application, and then decreased up to 40 days, thereafter kept a certain level. The available phosphorous accumulated at 0~20cm depth in loam soil, and 0~50cm in sandy loam soil. Annual accumulation rates were 17% higher in sandy loam soil than loam soil. The more compost application rates and times, the higher base saturation percentage increased in upland soils. Four year's application at a rate of $80Mg\;ha^{-1}$ per year increased the base saturation percentage to 87~91% compared with 45% at control plot in the loam soil. While in sandy loam soil only three year's application of same rate increased the base saturation percentage to 81~92% compared with 30.4% at control plot. The average annual increasing rate of base saturation percentage at the same application rates of composts were higher in sandy loam soil by 2.0~3.7 times than in loam soil. The application of compost increased the exchangeable Ca, Mg, and K contents of soils by 2, 2~3, and 3~5 times, respectively, compared with the control. The contents of exchangeable canons were high in surface soil. and decreased with increase of soil depths. In the case of heavy metal content, there were no difference at the application of PMC and CMC but Ni. Fe, Zn, Cu was increased a little when the HES applied, and Ni and Cr was increased application with FISC.
Journal of The Korean Society of Grassland and Forage Science
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v.29
no.3
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pp.211-216
/
2009
Approximately 43 million tons of livestock manure (LM) are produced each year on Korean farms. LM can be utilized as a valuable resource and/or it can contaminate water by runoff and leaching through the soil, when LM has been thoughtlessly applied to the land and directly discharged into the water. This experiment was carried out to investigate the effect of no-till system and LM application on dry matter (DM) yield of silage corn and $NO_3$-N concentration in leaching water of lysimeter installed in the experimental field. The treatments were replicated three times in split plot design. Main plots consisted of tillage systems, such as conventional tillage (CT) and no-tillage (NT). Sub plots consisted of the type of LM, such as chemical fertilizer (CF), composted cattle manure (CCM) and composted swine manure (CSM). The control plots were fertilized as commercial chemical fertilizer. DM yields of corn increased significantly in order to CF > CCM > CSM (p<0.05). DM yield of corn in CT increased as comparing with that of corn in NT. Plant height, ear height and stem diameter also increased in order to CF > CCM > CSM. In addition, the root weight in CT was increased as comparing with that of corn in NT. However, there was no interaction effects of between type of LM and tillage system. $NO_3$-N concentration in leaching water of LM application was less than 10 ppm, but $NO_3$-N concentration in CF exceeded 10 ppm which is safety level of drinking water during summer time (rainfall season).
Journal of The Korean Society of Grassland and Forage Science
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v.30
no.1
/
pp.49-58
/
2010
This experiment was carried out to estimate the nutritive value of mixed-sowing winter forage crop and whole crop barley by investigating the effects of feeding mixed-sowing winter forage crop and whole crop barley in KBG (Korean black goat) on feed intake, average daily body weight gain, nutrient digestibility and nitrogen retention. The 12 male KBG were divided into four experimental groups - i.e. T1: barley silage added group, T2: mixed-sowing hairy vetch silage added group, T3: mixed-sowing field peas added silage, T4: mixed-sowing Italian rye grass added silage group. Three KBG per each treatment were allotted into individual metabolic cages by Latin-square design. The results from this study are as follow. The DMI (dry matter intake) and organic matter intake in T4 were significantly (p<0.05) higher than those in T1 and T3 and crude protein intake in T4 also recorded the highest among treatments (p<0.05). The highest values in intakes of ADF and NDF were observed in T4 followed by T2, T1 and T3 in order. The average daily body weight gains in T4 and T2 were significantly (p<0.05) higher than those in TI and T3. The dry and organic matter digestibility in T2 and T4 were significantly (p<0.05) higher than those in T1 and T3. The crude protein digestibility in T1 was significantly (p<0.05) lower than those in T2 and T4. The nitrogen intakes in T2 and T4 were significantly (p<0.05) higher than those in T3. The results obtained from this study suggested that the feeds supplemented with whole crop barley with hairy vetch and Italian rye grass mixture silage increased KBG productivity resulted from increases in feed intake, nutrient digestibility and nitrogen retention.
The purpose of this study was investigated the quality changes before and after harvesting, storage and, processing of onion. Experiments were carried out to compare the effect on the characteristics of the postharvest from preharvest factors using onion. This experiment had identified the characteristics of harvested onions after cultivating with several preharvest factors such as the light and water conditions. These tests were conducted in an onion growth in the field, storage, and processing of fresh-cut during a laboratory periods of 2 years. In first year, onion cultivars ('Kars' and 'Pop') were produced under stable or unstable environment conditions, these onions were stored at low temperature(0?). Measurement was evaluated by the growth amount after harvesting, and the fresh weight loss and respiration rate during storage. According to different culture conditions and storage temperatures, it was investigated the properties of the fresh-cut onion. Growth of onion was varied depending on the cultivars and culture conditions. The amount of growth on 'Kars' and 'Pop' onions were decreased by excessive soil water conditions with shading. These influences were found the morphological differences resulting for the cell tissue of onion being rough and large. Onion cultivated in excessive soil water with shading affected the degree of its respiration rate and fresh weight loss during storage. Ones in excessive soil water with shading were higher than the control in fresh weight loss and respiration rate, respectively. However fresh-cut onion could not investigated to clarify the difference due to effects of cultivation condition and storage temperature on some measure items such as electrolyte leakage and microbial number change. There was a change of only electrolyte leakage depending on the storage temperature, rather than cultivated conditions before harvesting factor. The results showed that the onion grown on in the good environment was represented to a good quality produce even after harvesting.
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