• Asian-Australasian Journal of Animal Sciences
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• v.25 no.6
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• pp.818-823
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• 2012

Tall fescue (Festuca arundinacea Schreb.) is an important cool season forage plant that is not well suited to extreme heat, salts, or heavy metals. To develop transgenic tall fescue plants with enhanced tolerance to abiotic stress, we introduced an alfalfa Hsp23 gene expression vector construct through Agrobacterium-mediated transformation. Integration and expression of the transgene were confirmed by polymerase chain reaction, northern blot, and western blot analyses. Under normal growth conditions, there was no significant difference in the growth of the transgenic plants and the non-transgenic controls. However, when exposed to various stresses such as salt or arsenic, transgenic plants showed a significantly lower accumulation of hydrogen peroxide and thiobarbituric acid reactive substances than control plants. The reduced accumulation of thiobarbituric acid reactive substances indicates that the transgenic plants possessed a more efficient reactive oxygen species-scavenging system. We speculate that the high levels of MsHsp23 proteins in the transgenic plants protect leaves from oxidative damage through chaperon and antioxidant activities. These results suggest that MsHsp23 confers abiotic stress tolerance in transgenic tall fescue and may be useful in developing stress tolerance in other crops.

• Journal of the Korean Institute of Landscape Architecture
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• v.31 no.4
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• pp.74-81
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• 2003

The need for insect and mite resistant turfgrass cultivars arose because of problems associated with pesticide use. Representative cultivars and genotypes of 18 warm-season turfgrass [Zoysia japonica Steud., Z. japonica${\times}$Z. metrella hybrids, Z. japonica${\times}$Z. tenuifotia hybrids, Z. matrella (L.) Merr., Cynodon dactylon (L.) Pers., C. dactylon${\times}$C. transvallensis hybrids, Paspalum notatum Flugge., P. vaginatum Swartz., Stenotaphrum secundatum (Walt.) Kuntze, Eremochloa ophiuroides (Munro.) and Buchloe dactyloides (Nutt.) Engelm.] and 20 cool-season turfgrasses [Poa pratensis L., Festuca arundinacea Schreb., F. rubra L., F. rubra var. commutata Gaud., F. ovina var. duriuscula L. Koch. Agrostis tenuis Sibth., A. palustris Huds., and Latium perenne L.] were evaluated for host resistance to feeding by the Spodoptera depravata (Butler) in the laboratory. Two experiments were set up in the laboratory using 8.5cm diameter${\times}$4.0cm deep plastic petri dishes as larvae feeding chambers. In experiment 1, one neonate larvae were place on the grass in each dish and the dishes were arranged with 5 replicates each within an environmental chamber maintained at $25^{\circ}C$ and 15h light: 9h dark Larval survival and larval weights at 7d and 14d, pupal weights, and days to pupation were compared among turfgrasses. In Experiment 2, 4cm sections of all grasses were oriented equidistant from each other in a pattern resembling the spokes of a wheel. Five one neonate larvae were introduced to the center of each dish. Dishes were immediately placed in an environmental chamber held at $25^{\circ}C$, 15h light: 9h dark Larvae were allowed to feed for 24h. Damage was rated from 0(no damage) to 9(completely consumed) were made for eachturfgrass. Resistance as antibiosis (high mortality, slowed growth, and least preference) was identified in Z. japonica${\times}$Z. tenuifolia hybirids ‘Emerald’, Z. japonica${\times}$Z. metrella hybirds ‘Miyako’ and Eremochloa ophiuroides (Munro.). Cool-season turfgrasses tested were susceptible to feeding by Spodoptera depravata (Butler).

• Journal of the Korean Society of Environmental Restoration Technology
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• v.21 no.3
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• pp.47-56
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• 2018

Research was initiated to investigate germination characteristics and germination pattern of 14 herbaceous plant entries used on the road slope during 30 days. An alternative germination condition for 14 herbaceous plant entries required by International Seed Testing Association(ISTA) was applied in the experiment, consisting of 8-hr light at $25^{\circ}C$ and 16-hr dark at $15^{\circ}C$. Significant differences were observed in the first germination rate(0.3%~40.7%)and in the final germination rate(7.7%~93.3%). Days to the first germination(2~8days), days to the 30% germination(2~6days) and days to the peak germination(6~18days) were different among 14 herbaceous plant entries in the study. From this result, we could find out higher final germination rate of 14 herbaceous plant entries in the following order; First, forage crops and cool-season turfgrasses; Second, herbeceous flowers; Third, wild plants. We could also divide germination rate among 14 herbaceous plant entries as 6 groups(I;very high, II;high, III;medium high, IV;medium low, V;low, VI;very low) based on the final germination rate and divide germination speed as 5 groups(A;very fast, B;fast, C;normal, D;slow, E;very slow)based on days to the peak germination. Considering germination characteristics and pattern of 14 herbaceous plant entries Medicago sativa, Lolium perenne, Festuca arundinacea and Cosmos sulphureus were regard as dominating species while Lespedeza cuneata, Silene armeria, Lotus corniculatus var.japonicus, Coreopsis tinctoria and Centaurea cyanus as competitive species following dominating species. However, Chrysanthemum burbankii, Pennisetum alopecuroides, Chrysanthemum boreale., Artemisia princeps var. orientalis and Arundinella hirta were not almost expected to emerge.

• Journal of Korean Society of Forest Science
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• v.98 no.5
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• pp.539-547
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• 2009

This study was conducted to investigate the arbuscular mycorrhizal fungal biodiversity, spore density and root colonization in relation to site ages at expressway cut-slopes in Korea. Stabilization of exposed surface involved soil amendments and spraying seed mixture of turf grasses and/or nitrogen-fixing shrub species. Eighteen sites were selected with varying ages (2 to 16 years). Soil samples collected in October from each site were analyzed for fungal diversity and spore counts. Fine root samples from the plants were assayed for fungal colonization. Of the total 37 plants inspected in the sites, 26 species had endomycorrhizal colonization with an average root colonization rate of 18%, and with a range from 1 to 67%. The average endomycorrhizal colonization rate of initially introduced Festuca arundinacea which became the most dominant grass in later stage showed 22.8%, while that of Lespedeza bicolor which became the most dominant woody species were 21.6%. Naturally-invading Robinia pseudoacacia showed higher colonization rate in the old sites. Although site age did not show significant effects on fungal diversity, the root colonization rates of initially introduced plants decreased with the site aging, while those of invading plants increased with aging of the sites. The soil chemical properties, pH, N, and P contents, were negatively correlated with spore density, root colonization and endomycorrhizal species diversity. A total of forty arbuscular mycorrhizal fungal species in seven genera were identified. Of the 40 species, Acaulospora lacunosa, Glomus aggregatum, Glomus constrictum, Scutellospora erythropa, and Acaulospora spinosa were the five most dominant species in the decreasing order.

• Korean Journal of Environment and Ecology
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• v.21 no.1
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• pp.1-12
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• 2007

Nauralized plants in Ulleungdo were enlisted as 65 taxa, 21 families,49 genera,63 species and 2 varieties by field survey and flora lists of literature cited. Family Compositae covered 27.7% and Family Gramineae, Family Leguminosae and Family Polygonaceae covered 7.7% each. In this study, the nine taxa such as Rumex nipponicus, Trifolium campestre, Oxalis articulata, Anthriscus caucalis, Ipomoea purpurea, Coreopsis Ianceolata, Galinsoga parviflora, Tritonia crocosmaeflora and Festuca arundinacea are added as Nauralized plants in Ulleungdo. Annual plants ratio is 36.9% by life-form spectrum and Europe ratio is 40.0% by origin. The Urbanization Index was 22.0 and the Ratio of Naturalized has increased by flora lists of literature cited each. Ambrosia artemisiifolia var. elatior is growing in Ulleungdo by a plant cause disturbance. Spread prevention and minimization incoming of gardening plants by management plan of naturalized plants might be required. Moreover, it is needed to induce the succession for the recovery of the cut-slope using the native plants.

• Asian Journal of Turfgrass Science
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• v.24 no.1
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• pp.36-44
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• 2010

We were investigated turf color and Normalized Difference Vegetation Index (NDVI) to affect greenup promotion of 38 cultivar in 5 species for spring season, most popularly used in Korea golf courses. Kentucky bluegrass (Poa pratensis L.), tall fescue (Festuca arundinacea Schreb), creeping bentrgass (Agrostis palustris Huds), perennial ryegrass (Lolium parenne L.) and zoysiagrass (Zoysia spp.) cultivar had higher leaf color for greenup promotion in Ami, Amx and Oligo than Con treatment and there were also significantly difference among treatments. Turfgrass NDVI also showed significant difference among cultivars in species. Spray of Ami, Amx and Oligo has the higher value of turf NDVI than Con. Based on our results, green up fast during spring season may affected much more with application of Ami, Amx and Oligo than Con.

• Asian Journal of Turfgrass Science
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• v.24 no.1
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• pp.50-55
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• 2010

Five turfgrass species and 46 cultivars were investigated for difference of spring greenup and living ground cover. Turf color and Normalized Difference Vegetation Index (NDVI) for greenup were investigated between species and cultivars. Turf color and NDVI were showed significantly different among species and cultivars. Turf color was showed significantly different among 20 cultivars of kentucky bluegrass (Poa pratensis L.). NDVI was significantly difference among 20 cultivars of kentucky bluegrass and 6 cultivars of tall fescue (Festuca arundinacea Schreb). The percentage living ground cover was showed significantly different among creeping bentgrass (Agrostis palustris Huds) cultivars and fineleaf fescue cultivars in spring.

• Asian Journal of Turfgrass Science
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• v.2 no.1
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• pp.5-30
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• 1988

The object of this experiments was to know the salt tolerance of Fults and other lawn grasses. Fults, Olympic (Tall fescue, Festuca arundinacea Schred), Ceeping red fescue, Kentucky bluegrass and Zoysia grass (Z. japonica S.) were grown in hydroponics with vermiculite at various concentrations of NaCl. Hoagland's solution was used as the basic solution (control), and the concentrations of Cl to it were 1000, 2000, 3000, 4000 and l5OOOppm, respectively. Each was cultivated under the circumstances during 2 months. The results obtained are summarized as follows: 1.The growth of Fults, Olympic, Creeping red fescue and others were better at Cl lOOOppm than control. In the 5OOOppm application, Fescues become worse and 23.9% of them were withered. In concentration of Cl above 9OOOppm, it was impossible to live. 2.In the l0000~l1000ppm application, Olympic and Kentucky bluegrass were become worse and most of them died. 3.Fults were almost possible to live in the below of 9OOOppm, but they began to die in the above of 10000 ppm. 4.With the increasing concentration of Cl, plants were dwarfed and the number of stems, leaves and roots were reduced, but it was especially observed that the number of stolons of Creeping red fescue were increased at 1000~4000ppm. 5.Fults grass was the most salt tolerant turfgrass, but was impossible to live at salt level of about 36 millimhos (Exchange NaCI conductivity-ppm). Among the grasses, according to salt tolerance, they were arranged as follows. Fults > Zoysia japonica S. > Ky belugrass > Olympic grass > Creeping red fescue 6.The number of leaves, stems, tillers, and dry weight of Olympic grass, Fults and others were increased more at Cl 1OOOppm application than control, but in the above 4OOOppm application, those of plants were decreased. 7.The productivity of all grasses under the experiments was increased at 3.l75millimhos (Exchange NaCi conductivity ppm) in the concentration of Cl. The each dry-weight of Olympic, Creeping red fescue, Kentucky bluegrass and Zoysia grass was decreased at 8.85millimhos, and the weight of Fults grass was also decreased remarkably at 12.20millimhos. 8.As the result of this experiments, most plants grow normally at low concentration of NaCI-l000ppm. That seems to stimulate more the grasses to grow than non-salt.

• Journal of The Korean Society of Grassland and Forage Science
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• v.37 no.3
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• pp.223-230
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• 2017

Arabidopsis nucleoside diphosphate kinase 2 (AtNDPK2) is an upstream signaling molecule that has been shown to induce stress tolerance in plants. In this study, the AtNDPK2 gene, under the control of a stress-inducible SWPA2 promoter, was introduced into the genome of tall fescue (Festuca arundinacea Schreb.) plants. The induction of the transgene expression mediated by methyl viologen (MV) and NaCl treatments were confirmed by RT-PCR and northern blot analysis, respectively. Under salt stress treatment, the transgenic tall fescue plants (SN) exhibited lower level of $H_2O_2$ and lipid peroxidation accumulations than the non-transgenic (NT) plants. The transgenic tall fescue plants also showed higher level of NDPK enzyme activity compared to NT plants. The SN plants were survived at 300 mM NaCl treatment, whereas the NT plants were severely affected. These results indicate that stress-inducible overexpression of AtNDPK2 might efficiently confer the salt stress tolerance in tall fescue plants.

• Journal of Animal Science and Technology
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• v.63 no.2
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• pp.305-318
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• 2021

Alfalfa (Medicago sativa L.) is an important forage legume grown in Kansas, USA and its productivity with cool-season grasses however is unknown. The objective of this study was to determine the dry matter yield (DMY) and forage nutritive value of alfalfa-grass mixtures compared to those of alfalfa and grasses grown in monoculture with and without nitrogen fertilization. Three different alfalfa varieties were planted (reduced-lignin alfalfa, Roundup Ready, and conventional alfalfa) and two kinds of cool-season grasses (smooth brome, Bromus inermis Leyss, and tall fescue, Festuca arundinacea Schreb) were planted as a monoculture or in alfalfa-grass mixtures. Nitrogen fertilizer (urea) was applied at green-up at a rate of 56 kg/ha and after the second cutting at a rate of 56 kg/ha in 2016 and 2017, respectively. and control treatments received no nitrogen. DMY was significantly higher in monoculture alfalfa and alfalfa-grass mixtures than in grass monocultures. Between alfalfa monoculture and alfalfa-grass mixtures, no significant differences in DMY were found. For all treatments, nitrogen application significantly increased DMY compared to the control. In 2016 and 2017, the low-lignin alfalfa monoculture had the lowest acid detergent fiber (ADF) and the grass monocultures had the highest ADF. In 2016 and 2017, neutral detergent fiber (NDF) in smooth bromegrass and tall fescue was higher than in other species treatments. A low-lignin alfalfa monoculture had significantly lower NDF concentration compared to alfalfa-grass mixtures. When averaged over 2016 and 2017, relative feed value (RFV) was highest in low-lignin alfalfa and lowest in the grass monocultures. In both years, nitrogen fertilizer application did not affect nutritive values.