• 농업생명과학연구
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• 제46권2호
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• pp.115-127
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• 2012

Antioxidant and neuronal cell protective effects of aqueous extract from lotus (Nelumbo nucifera) leaf tea (LLTE) were investigated. The 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) radical scavenging effect, ferric reducing antioxidant power, and malondialdehyde inhibition of LLTE were increased in a dose dependent manner. Intracellular reactive oxygen species accumulation resulting from hydrogen peroxide ($H_2O_2$) treatment was significantly reduced when LLTE were present in the media compared to PC12 cells treated with $H_2O_2$ only. In neuronal cell viability assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide (MTT), LLTE showed protective effect against $H_2O_2$-induced neurotoxicity. In addition, lactate dehydrogenase release into medium was also inhibited by LLTE (7.13-43.89%). Total phenolics of LLTE were 33.16 mg/g and a quercetin was identified as major phenolics (105.93 mg/100g). Therefore, above these data suggest that LLTE including quercetin may be useful in the natural antioxidant substance, and may reduce the risk of neurodegenerative disease.

• 한국축산식품학회지
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• 제44권5호
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• pp.1126-1141
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• 2024

Antioxidant activity of freeze-dried paprika powder and storage properties of emulsion-type pork sausages containing diverse concentrations of this powder (0%, 1%, 2%, and 3%) were analyzed. Antioxidant activities of red and yellow paprika powders were analyzed by evaluating their 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity, ferric reducing antioxidant power (FRAP), total phenol content (TPC), and total flavonoid content (TFC). The yellow paprika powder exhibited remarkably higher DPPH radical scavenging activity, FRAP values, and TPC than the red paprika powder (p<0.05), while TFC showed no remarkable difference between them (p>0.05). Storage properties of sausages containing the yellow paprika powder were analyzed by evaluating their water holding capacity, cooking yield, and thiobarbituric acid reactive substance (TBARS), and volatile basic nitrogen (VBN) values. The 3% yellow paprika powder group showed remarkably higher water-holding capacity and cooking yield compared to the 0% group (p<0.05). TBARS values were remarkably lower in the 2% and 3% yellow paprika powder groups than in the 0% group at all weeks (p<0.05). VBN value was remarkably lower in the 3% yellow paprika powder group than in the 0% group at all weeks (p<0.05). Overall, addition of 3% yellow paprika powder improved the storage properties of emulsion-type sausages.

• 대한화장품학회지
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• 제48권4호
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• pp.357-364
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• 2022

본 연구에서는 청태전 추출물을 이용하여 항산화 관련 기능성 화장품 소재로서의 활용가치를 검증하고자 항산화 효능을 분석하였다. 열수와 70% 에탄올을 용매로 청태전을 추출, 농축, 동결건조하여 시료를 제조한 다음 ABTS⁺ radical 소거능, 전자공여능, SOD 유사 활성능, 환원력, FRAP 환원성 항산화활성, 총 페놀 및 플라보노이드 함량 분석을 진행하였다. ABTS⁺ radical 소거능 분석 실험의 경우, 청태전 열수 추출물(CTW), 청태전 70% 에탄올 추출물(CTE) 모두 1,000 ㎍/mL의 농도에서 98% 이상의 소거 활성을 나타내었으며, 전자공 여능 분석 실험의 경우, CTW, CTE 각각 42.20%, 78.82%의 활성을 나타내었다. SOD 유사 활성능 측정 결과, CTW, CTE 각각 39.73%, 67.39%의 활성이 확인되었다. FRAP, Reducing power 실험의 경우, CTW, CTE 모두 높은 효과를 나타내었고, 총 페놀 및 플라보노이드 함량 분석 결과, CTW, CTE 모두 높은 함량을 나타내었다. 따라서 청태전 추출물은 항산화 관련 기능성 화장품 소재로서 활용가치가 높을 것으로 사료된다.

• 농업생명과학연구
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• 제45권4호
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• pp.113-120
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• 2011

본 연구에서는 생마늘 추출 숙성물의 용매 분획물에 대한 항산화 효과와 acetylcholinesterase (AChE) 저해 효과에 대해서 조사하였다. 생마늘 추출 숙성물의 헥산, 클로포름, 에틸아세테이트 및 물 분획물을 통한 총 페놀성 화합물의 함량은 각각 3.70, 23.63, 31.27, 2.35 mg/g으로 나타났다. 또한, 에틸아세테이트 분획물이 ABTS radical scavenging activity, ferric reducing antioxidant power 및 linoleic acid를 활용한 지질의 자동산화 저해 효과에서 가장 높다는 것을 확인하였다. 추가적으로, PC12 신경 세포에서의 에틸아세테이트 분획물의 처리가 $H_2O_2$가 유발시키는 산화적 스트레스의 수준을 감소시켰다. 결국 생마늘 추출 숙성물의 에틸아세테이트 분획물이 농도 의존적으로 AChE를 저해한다는 것을 확인하였다. 그러므로 본 연구는 생마늘 추출 숙성물의 에틸아세테이트 분획물이 PC12 신경세포에 있어서 산화적 스트레스 수준을 감소시키고, 또한 AChE 저해제로서 활용될 수 있는 가능성을 확인하였다.

• 생명과학회지
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• 제32권9호
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• pp.667-677
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• 2022

돌산갓 종자를 원형, 찧은 형태, 볶은 형태로 각각 0.5 g (S-1), 1.0 g (S-2), 1.5 g (S-3) 첨가한 국물용 육수팩를 10분, 15분간 가열한 육수를 냉각한 후 품질적 특성을 측정하였다. 가열시간이 짧을수록 pH와 염도는 감소하는 경향을 나타냈다. 항산화활성은 총폴리페놀, 총플라보노이드, 전자공여능(EDA), ABTS radical 소거활성 및 ferric reducing antioxidant power (FRAP) 항목으로 측정하였다. 전체적으로 항산화활성은 S-1보다 S-2와 S-3에서 높은 함량을 나타났고, 가열시간이 길수록 증가했다. Sinigrin 함량은 대조군에서 검출되지 않았고, 시료간 유의적 차이가 없었다. 총아미노산은 가열시간이 길수록 볶은 샘플에서 glutamic acid의 함량이 높게 나타났고, aspartic acid, glycine, proline, alanine, arginine등이 높은 함량으로 검출되었다. 유리아미노산 함량은 감칠맛 계의 aspartic acid, glutamic acid함량이 높게 나타났다. 휘발성 성분들은 갓종자를 첨가한 육수는 2-propenyl- isothiocyanate (ITC) (C4H5NS), allyl thiocyanate (C4H5NS), n-butyl ITC (C5H9NS), 3-butenyl ITC (C5H7NS) 등이 검출되었다. 관능검사는 돌산갓 종자 첨가량과 가열시간이 길수록 높은 점수를 받아 전체적인 기호도가 증가하였고 시료간의 유의적인 차이를 나타냈다. 돌산갓 종자를 첨가한 육수의 품질적 특성을 토대로 돌산갓 종자를 첨가한 제품 개발을 위한 기초자료로 제시하고자 한다.

• 한국수산과학회지
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• 제53권6호
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• pp.886-893
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• 2020

The seaweed Hizikia fusiformis is rich in protein, carbohydrates, vitamins, and minerals. This study investigated the antioxidant and physiological activities of H. fusiformis extracts prepared with 70% ethanol, 80% methanol, or distilled water. The extraction yields of these various solvent extracts were as follows: ethanol extract, 15.26%; methanol extract, 17.95%; and water extract, 45.62%. The methanol extract showed the highest total polyphenol content (24.06 mg GAE/g), but total flavonoid content was similar in all extracts. ABTS (2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) radical scavenging activity was highest in the ethanol extract (IC50: 0.90 mg/mL), while the methanol extract exhibited the strongest DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity (IC50: 8.09 mg/mL), reducing power (EC50: 0.40 mg/mL), and ferric reducing antioxidant power (0.28 mM). By contrast, tyrosinase and α-glucosidase inhibitory activities were higher in the ethanol extract than in the other extracts. The high BACE1 (β-secretase) inhibitory activity was observed in the ethanol extract (IC50: 1.03 mg/mL). These results indicate that H. fusiformis ethanol extracts may be useful for their antioxidant and functional properties in food and pharmaceutical materials.

• 한국수산과학회지
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• 제55권6호
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• pp.858-866
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• 2022

The present study investigated the chemical composition, and antioxidant and physiological activities of the Korean marine algae, Codium fragile. The solvent extracts from C. fragile were prepared using 70% ethanol, 80% methanol, and distilled water. Based on the general chemical composition, carbohydrate, crude protein, crude lipid, crude ash, and moisture were 74.22%, 16.73%, 0.66%, 4.39%, and 4.00%, respectively. Calcium, magnesium, sodium and potassium were the main minerals. The extraction yield range of the solvent extracts was 3.51-9.76%. The ranges of total polyphenol and flavonoid contents were approximately 10.97-13.76 mg/g and 8.00-8.69 mg/g, respectively. The ABTS [2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)] radical scavenging activity, reducing power and FRAP (ferric reducing antioxidant power) activities were the highest in the ethanol extract, while methanol extract exhibited the strongest nitrite oxide scavenging activity. On the other hand, tyrosinase, elastase, and xanthine oxidase inhibitory activities of the ethanol and methanol extracts were higher than those of the water extract. Furthermore, the ethanol extract exhibited the highest β-secretase inhibitory activity. The results indicate that C. fragile can be used as an antioxidant and a functional ingredient in food and pharmaceutical products.

• Journal of Plant Biotechnology
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• 제49권2호
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• pp.155-161
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• 2022

본 연구는 복분자 종자 에탄올 추출물의 항산화 및 항염증 활성 검증하였다. 항산화 활성을 알아보기 위하여 총 폴리페놀 및 플라보노이드 함량, ABTS 및 DPPH 라디칼 소거능과 환원력을 측정하였다. 복분자 종자 에탄올 추출물의 총 폴리페놀 및 플라보노이드 함량은 각각 4.09 ㎍ GAE/mg와 16.25 ㎍ QE/mg이었다. DPPH와 ABTS 라디칼 소거능은 농도 의존적 소거능을 보였고, 복분자 종자 에탄올 추출물의 RC50 값은 각각 26.68, 39.30 ㎍/mL이였다. 게다가 환원력을 평가하기 위해 수행된 FRAP 분석에서 복분자 종자 추출물은 0.61 ± 0.01 mM FeSO₄ E/mg의 효능을 보여주었다. 항염증 효과를 측정하기 위해 RAW 264.7 세포에서 세포독성과 산화질소(NO) 생성 억제 효능을 확인하였다. 확인 결과 500 ㎍/mL의 복분자 종자 추출물에서 세포 독성 없이 산화질소 생성 억제 효능을 확인할 수 있었다. 복분자 종자 추출물을 사용한 항산화 및 항염증 활성을 확인한 결과 항산화 및 항염증 물질로서의 가능성을 확인할 수 있었다.

• Preventive Nutrition and Food Science
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• 제19권2호
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• pp.75-81
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• 2014

This study was conducted to investigate the effects of cranberry power on antioxidant defense system in rats fed an atherogenic diet and injected with lipopolysaccharide (LPS). Sprague-Dawley rats were divided into the following 5 groups: normal diet+saline (NS), atherogenic diet+saline (AS), atherogenic diet+LPS (AL), atherogenic diet with 5% cranberry powder+LPS (AL-C5), and atherogenic diet with 10% cranberry powder+LPS (AL-C10). Total antioxidant status measured by ferric reducing ability of plasma (FRAP) was significantly reduced by LPS injection (24%) and was restored by the cranberry powder treatment (P<0.05). In addition, the mean level of plasma total phenolics was significantly decreased by LPS injection (P<0.05) and tended to be increased when cranberry powder was incorporated in to the diet. Activity of serum superoxide dismutase (SOD) tended to be lowered by LPS injection and declined further in cranberry powder fortified groups. Overall results indicate that dietary cranberry powder may provide appropriate antioxidants to counter the diminished antioxidant status induced by exposing hypercholesterolemic rats to LPS.

• Asian Pacific Journal of Cancer Prevention
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• 제13권5호
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• pp.1929-1933
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• 2012

Oxidative stress has been proposed to be involved in colorectal cancer development. Many dark pigments of plants have potent oxidative stress preventive properties. In this study, unpolished Thai rice was assessed for antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) methods. Red strain unpolished Thai rice was also administered to rats exposed to azoxymethane (AOM) for induction of aberrant crypt foci (ACF). Serum malondialdehyde (MDA) and ferric reducing antioxidant power (FRAP) were investigated for cellular oxidative stress and serum antioxidants, respectively. Red pigment unpolished Thai rice demonstrated high antioxidant activity and was found to significantly and dose dependently decrease the total density and crypt multiplicity of ACF. Consumption of Thai rice further resulted in high serum antioxidant activity and low MDA cellular oxidative stress. Interestingly, the density of ACF was strongly related to MDA at r = 0.964, while it was inversely related with FRAP antioxidants (r = -0.915, p < 0.001). The results of this study suggest that the consumption of red strain of unpolished Thai rice may exert potentially beneficial effects on colorectal cancer through decrease in the level of oxidative stress.