• Applied Microscopy
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• v.41 no.4
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• pp.229-234
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• 2011

The occurrence of nuclear inclusions has been reported in various plant groups from primitive ferns to higher flowering plants. Their presence within a group seems to be randomly distributed without any phylogenetic relationships among species. According to the current survey, nuclear inclusions have been widely documented in more than several hundreds of species from various families of plants. The morphology and internal structures of nuclear inclusions are diverse and at least five types of inclusions develop within plant nuclei; amorphous, crystalline, fibrous, lamellar, and tubular form. Among these types, crystalline inclusions are the ones that are the most frequently reported. The inclusions are not bound by membranes and appear to be related to the nucleoli, either spatially by a close association or by an inverse relationship in size during development. The idea that nuclear inclusions are of a proteinaceous nature has been widely accepted. Further link to nucleolar activity as a protein storing site has also been suggested based on the association between the nucleolus and nuclear inclusions. Various investigations of nuclear inclusions have revealed more information about their structural features, but characterizing their precise function and subunit complexity employing molecular analysis and 3-D reconstruction remains to be elucidated. Tilting and tomography of serial sections with appropriate image processing can provide valuable information on their subunit(s). The present review summarizes discussion about different nuclear inclusions in plants from previous works, giving special attention to their fine, ultrastructural morphology, function, and origin.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.8 no.1
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• pp.73-78
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• 2005

It was aimed to promote Cyrtomium falcatum as a material for interior landscape by validating it indoor adaptability in the indoor environment, especially irrigation times, soil treatment and drainage level. Irrigation times were 2 times per week and 7 times per week. Soil treatment and drainage level were carried out drainage-peatmoss : vermiculite : perlite=1 : 1 : 1(D-PVP), peatmoss : vermiculite : perlite=1 : 1 : 1(PVP), drainage-saprolite : leaf mold=1 : 1(D-SL) and saprolite : leaf mold=1 : 1(SL). 1. Top of growth was better with irrigation 7 times per week than irrigation 2 times per week but indoor adaptability was decreased and shown yellowish green. 2. In case of soil treatment, growth was better with saprolite : leaf mold=1 : 1 but indoor growth adaptability was decreased than peatmoss : vermiculite : perlite=1 : 1 : 1. 3. Plant height and blade length were increased under non-drainage treatment but indoor adaptability, number of new fronds and number of sporophyll were decreased under drainage treatment, regardless of irrigation times and soil treatment. 4. Photosynthetic rate(Pn) was the highest in the drainage-peatmoss : vermiculite : perlite=1 : 1 : 1 treatment of irrigation 2 times per week and was the lowest in the saprolite : leaf mold=1 : 1 treatment of irrigation 7 times per week.

• Journal of Life Science
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• v.27 no.2
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• pp.241-246
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• 2017

The walls of guard cells have many different specialized features. Guard cells are present in leaves of bryophytes, ferns and almost all of the vascular plants. Guard cells show considerable morphological diversities. It is understood that the stomata show two types in terms of morphological characterizations of guard cells. The first type is only found in a few monocots including Poaceae and Cyperaceae. In rice and corn, guard cells have the morphological characteristics of dumbbell shape. The morphological characteristics of dumbbell shape always have subsidiary cells. The other type is found in every dicots and many monocots and they are kidney-shaped guard cells. The plants of kidney-shaped guard cells rarely have subsidiary cells except Commelina communis L. Therefore, it could be concluded that two types of the morphological characteristics of guard cells cannot divide according to monocots or dicots. Every plants in which stomatal characteristic features were all different, most of them belong to kidney-shaped guard cells. However in case of Sedum sarmentosum, guard cells were shown to be long and narrow lips type. In Tradescantia virginiana, the shape of guard cells could be called perfectly to half-moon type. Therefore, it could be concluded that kidney-shaped types are all different in some way, but dumbbell-shaped types are almost constant.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.51-51
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• 2018

2015년 기준 우리나라의 고사리 재배면적은 3,276ha 수준으로 그중 전라북도의 고사리 재배는 재배면적 285ha이며 연간 생산량 1,985톤으로 전국 생산량의 21%를 차지하고 있어 전라북도의 산채 재배작목 중 중요한 비중을 차지하고 있다. 고사리(Ferns)는 고사리 속(Pteridium spp.)에 속하는 양치식물의 총칭으로서 우리나라에는 22과 70속 272종이 분포되어 있는 것으로 보고되었다. 고사리의 어린 순에는 가식부 100g 당 칼슘 15.0mg, 칼륨 185.0mg 등이 함유되어 있으며 골다공증, 심혈관질환 등에 효과가 있고 식이섬유로 인해 변비 예방에도 유용한 것으로 알려져 있다. 고사리 뿌리에서 추출한 전분은 중국 및 일본 등지에서 면류나 제과용으로 이용되고 있어 고사리 재배의 부가가치를 향상시킬 수 있을 것으로 기대되고 있다. 최근 인력 의존도가 상대적으로 적은 고사리 재배면적이 늘어나고 있는 추세이나 종근의 파종량에 대한 기준이 제시되지 않아 경영비 부담이 큰 실정이다. 본 연구는 고사리 종근의 파종량을 단위면적당 맹아수 기준 40~80개/$3.3m^2$ 범위에서 표고 500m의 허브시험장 연구포장에 3월 28일 정식한 후 고사리 신초의 특성 및 뿌리 전분의 특성에 대해 고찰하였다. 고사리 신초의 출현양상은 6월 26일 이후 빠르게 증가하여 7월 하순의 고온기에 완만해지는 경향을 보였고 누적 신초수는 맹아수 80개/$3.3m^2$ 파종구에서 80.5개를 보여 파종량을 높일수록 유의하게 증가하였다. 고사리 신초의 상품기준을 포자엽이 전개되지 않은 신초로서 신초장 15~20cm 범위로 정하는 경우 출현시기별로는 9월에 상품율이 높았고 종근 파종량간에는 유의한 차이가 없었다. 종근 파종량에 따른 단위면적당 뿌리 생산량은 70개/$3.3m^2$ 파종구와 80개/$3.3m^2$ 파종구에서 $1498.1g/m^2$와 $1490.3g/m^2$로 유의하게 많았으나 전분 추출 수율은 8.6~12.9% 수준을 보였고 파종량간에는 차이가 없었다. 단위면적당 전분 생산량은 80개/$3.3m^2$ 파종구에서 $176.2g/m^2$로 가장 높은 생산성을 보였으며, 고사리 전분의 호화특성에서 최고점도는 465.3RVU로 쌀 전분의 218.6 RVU에 비해 월등히 높았고 호화시간은 쌀 전분 6.0분에 비해 3.7분으로 짧은 특성을 보였다. 고사리 전분의 무기물 함향은 가식부 100g당 인산 20mg, 칼륨 47mg 수준으로 밀가루에 비해 낮았으나 칼슘은 밀가루 33mg과 대등한 32mg을 보였고 특히 철분에서는 밀가루 4mg 수준에 비해 31mg으로 높은 함량을 보여 밀가루의 철분을 보충할 수 있는 전분으로 유망하였다.

• Korean Journal of Plant Resources
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• v.23 no.1
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• pp.38-46
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• 2010

Phenolic compound contents and scavenging activity on DPPH and ABTS radicals in twelve fern species were studied by analysing ultrasonification extraction of dried aerial parts and rhizomes using methanol solvent. Total polyphenol content ranged from 2.97 to 140.1 mg per 1 g dried sample and 0.80 to $49.58\;mg{\cdot}g^{-1}$ fresh sample. Highest polyphenol content was obtained with aerial part of Davallia mariesii and Polystichum lepidocaulon. Total flavonoid content of dried sample was $2.56{\sim}34.91\;mg{\cdot}g^{-1}$ and fresh sample $0.71{\sim}11.49\;mg{\cdot}g^{-1}$. Higher flavonoids were obtained with dried aerial part of Dryopteris crassirhizoma, but with fresh aerial part of P. lepidocaulon. In general, total polyphenol content was higher in rhizomes, except in case of total flavonoid. Scavenging effects on DPPH and ABTS radicals was higher with rhizome extracts compared to aerial parts. The rhizome of Polystichum polyblepharum showed highest effects on both radicals. However, the amount of fresh sample for $RC_{50}$ with consideration of water content and extraction yield. P. lepidocaulon aerial part was more advantageous than P. polyblepharum rhizome. All the fern extracts except for the Coniogramme japonica extract demonstrated superior scavenging effects on ABTS radicals, being similar activity of ascorbic acid and BHT.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.71-71
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• 2019

고사리(Ferns)는 고사리 속(Pteridium spp.)에 속하는 양치식물의 총칭으로서 우리나라에는 22과 70속 272종이 분포되어 있는 것으로 보고되었다. 고사리의 어린 순에는 가식부 100 g 당 칼슘 15.0mg, 칼륨 185.0mg 등이 함유되어 있으며 골다공증, 심혈관질환 등에 효과가 있고 식이섬유로 인해 변비 예방에도 유용한 것으로 알려져 있다. 고사리 뿌리에서 추출한 전분은 중국 및 일본 등지에서 면류나 제과용으로 이용되고 있어 고사리 재배의 부가가치를 향상시킬 수 있는 방안으로 기대되고 있다. 본 연구에서는 고사리 뿌리는 가정용믹서기로 분쇄하는 방법과 고무망치를 사용해 파쇄한 방법에서 식품첨가제로 이용되고 있는 sodium metabisulfite 등 3종의 산화방지제를 사용하여 추출한 전분의 이화학적 특성을 조사하였다. 고사리 전분의 추출 수율은 분쇄하는 방법에서 높은 경향이었고 추출용액 중에서는 sodium metabisulfite 4% 용액에서 분쇄 처리 9.5%와 파쇄 처리 8.3%로 가장 높은 수율을 보였고, ascorbic acid 침지에서는 5.1%와 5.5%로 낮은 수율을 나타냈다. 추출한 전분의 색도값에서는 파쇄하는 처리에서 L 값이 높아지고 a와 b 값은 낮아지는 경향이었고 sodium metabisulfite 4% 용액 침지에서 분쇄와 파쇄 두 방법에서 가장 높은 명도값을 보였다. 고사리 전분의 추출방법별 Ca 함량은 분쇄 처리에 파쇄 처리에서 높아지는 양상을 보였고 파쇄 후 ascorbic acid 4% 용액에서 추출하는 처리에서 0.23%로 가장 높았으며, Mg 함량도 분쇄 처리에 비해 파쇄 처리에서 경미하게 높은 양상을 보였다. 유효태 Fe 함량 또한 파쇄처리에서 높은 경향을 보였고 추출 용액중에서는 ascorbic acid 4% 용액이 0.12%의 높은 함량을 보였다. 고사리 전분의 추출방법별 호화특성에서 최고 점도는 증류수, ascorbic acid 및 sodium carbonate 추출에서는 파쇄하는 방법에서 높아지는 경향이었지만 sodium metabisulfite 추출에서는 감소하는 경향을 보였다. 강하점도는 모든 추출용액에서 분쇄 처리에 비해 파쇄 처리에서 현저하게 증가하는 양상을 보였고 치반점도는 sodium metabisulfite와 ascorbic acid 추출은 파쇄처리에서 83.9 RVU와 53.0RVU로 낮아지는 경향을 보인 반면 sodium carbonate와 증류수 추출에서는 증가하는 양상을 나타냈다. 호화시간은 분쇄 처리가 파쇄 처리에 비해 약간 늘어나는 경향이었고 추출용액 간에는 큰 차이가 없었으며 호화온도는 분쇄처리가 파쇄 처리에 비해 높아지는 결과를 나타냈으며 파쇄한 고사리 뿌리를 증류수를 이용해 추출한 전분에서 가장 낮은 $69.7^{\circ}C$의 호화온도를 보였다.

• Korean Journal of Environment and Ecology
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• v.35 no.1
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• pp.1-23
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• 2021

This study conducted a survey on flora at Mt. susdolbon(508m), Mt. seongjubong (625.3m), and Mt. sulibong(596.2m), located in Yanggu-gun, Gangwon-do. A total of 403 taxa, including 6 forms, 43 varieties, 4 subspecies, 350 species, 255 genera, and 84 families, were surveyed in the three mountain areas. 18 taxa from 5 families of ferns, 3 taxa from 1 family of gymnosperms, and the dicotyledonous plants as 302 taxa from 69 families of the dicotyledonous plants were identified. The remarkable plants included 11 taxa of the Korean endemic plants, such as Populus tomentiglandulosa, Salix koriyanagi, Cimicifuga heracleifolia, Aconitum pseudolaeve, Clematis trichotoma, Echinosophora koreensis, Vicia chosenensis, Salvia chanryoenica, and Hemerocallis hakuunensis. The rare plants designated by the Korea Forest Service were 4 taxa, including Echinosophora koreensis as endangered species (EC), Cimicifuga heracleifolia, Epimedium koreanum, and Lilium cernuum. The Least Concern species (LC) were 13 taxa, including Persicaria orientalis, Exochorda serratifolia, Viola diamantiaca, Lithospermum erythrorhizon, and Trigonotis icumae. The invasive alien plants were 28 taxa, including Persicaria orientalis, Rumex crispus, Phytolacca americana, Cerastium glomeratum, Chenopodium album, Chenopodium ficifolium, Amorpha fruticosa, Robinia pseudoacacia, Trifolium pratense, Trifolium repens, Oxalis dillenii, Euphorbia supina, and Oenothera biennis, accounting for 6.9 percent of the 403 taxa of the surveyed plants.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.66 no.4
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• pp.271-278
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• 2021

Azolla is a genus of small aquatic ferns native to Asia, Africa, and America. Azolla is potentially significant as a nitrogen source in agriculture, particularly in conjunction with rice (Oryza sativa L.) cultivation. In addition, Azolla may either control weeds or act as a weed depending on management; therefore, we aimed to determine the effect of Azolla incorporation on weed (e.g., Monochoria vaginalis, Rotala indica, Aeschynomene indica, and Cyperus amuricus) control and its other benefits during rice cultivation. The effects of Azolla on weed emergence were evaluated based on plot area coverage by the Azolla mat, which appears as a green sheet over water, and the rate of weed emergence in paddy field. Weed occurrence was investigated 40 days after Azolla inoculation. Soil samples from the experimental plots were collected following harvest in 2014-2015, and analyzed. Moreover, rice growth, yield, and grain quality were measured. Azolla suppressed weed growth by 72%. Azolla mat incorporation in rice fields suppressed weed growth by over 60%. In addition, Azolla promoted nitrogen fixation. Compared with nitrogen management using chemical fertilizers, Azolla incorporation showed the potential for various positive effects, including the improvement of soil fertility by increasing total nitrogen, and organic matter content. Rice grown with Azolla maintained its yield without additional herbicide treatment. Moreover, the palatability of cooked rice was better when grown with Azolla.

• Korean Journal of Environment and Ecology
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• v.36 no.1
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• pp.30-55
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• 2022

This study conducted a flora survey in the areas of Mt. Sambong (282m) and Mt. Jaung (263m), located in Paju-si, Gyeonggi-do. A total of 434 taxa, including 6 forms, 34 varieties, 11 subspecies, 383 species, 271 genera, and 94 families, were surveyed in the two mountain areas. The ferns were identified as 24 taxa from 10 families, the gymnosperms as 8 taxa from 2 families, the dicotyledonous plants as 322 taxa from 73 families, and the monocotyledonous plants as 80 taxa from 9 families. Five taxa, including Salix koriyanagi, Cimicifuga heracleifolia, Forsythia koreana, Paulownia coreana, and Hemerocallis hakuunensi, were identified as the Korean endemic plants. Eight taxa, including Cimicifuga heracleifolia, Tylophora floribunda, and Iris minutiaurea as Vulnerable species, andAristolochia contorta, Viola diamantiaca, Lithospermum erythrorhizon, Lilium distichum, andArisaema heterophyllumas Least Concern species, were designated as rare and endangered plants designated by the Korea Forest Service. The invasive alien plants were 40 taxa, including Rumex crispus, Phytolacca americana, Cerastium glomeratum, Silene armeria, Chenopodium album, Chenopodium ficifolium, Magnolia obovata, Potentilla supina, Amorpha fruticosa, Medicago sativa, Robinia pseudoacacia, Trifolium repens, Euphorbia supina, Hibiscus trionum, and Oenothera biennisaccounting for 9.2 percent of the 434 taxa of the surveyed plants. As this area is a mountainous area close to settlement facilities, it is believed that ecological preservation measures and measures of specialized plants should be implemented.

• Korean Journal of Environment and Ecology
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• v.37 no.1
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• pp.35-58
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• 2023

In this study, we conducted a flora survey at Mt.Bakdal (363m) and Mt. Myeongbong (245.3m) in Paju-si, Gyeonggi-do. A total of 405 taxa, including 2 forms, 32 varieties, 8 subspecies, 363 species, 261 genera, and 87 families, were surveyed in the two mountain areas. The ferns were identified as 12 taxa from 6 families, the gymnosperms as 6 taxa from 2 families, the dicotyledonous plants as 290 taxa from 70 families, and the monocotyledonous plants as 97 taxa from 9 families. The remarkable plants included 8 taxa of the Korean endemic plants, which were Populus tomentiglandulosa, Salix koriyanagi, Clematis brachyura, Viola seoulensis, Forsythia koreana, Paulownia coreana, Weigela subsessilis, and Hemerocallis hakuunensis, and 3 taxa of the rare and endangered plants as designated by the Korea Forest Service, including Tylophora floribunda as Vulnerable (VU) species, and Viola albida and Chionanthus retusus as Least Concern (LC) species. The invasive alien plants were 35 taxa, including Rumex crispus, Phytolacca americana, Cerastium glomeratum, Chenopodium album, Chenopodium ficifolium, Lepidium virginicum, Amorpha fruticosa, Robinia pseudoacacia, and Trifolium repens, accounting for 8.5 percent of the 405 taxa of the surveyed plants. We intend to further identify plants through the studies on unsurveyed areas in the western part of the DMZ, identify the native habitats of rare and specialty plants, and use them as basic data for managing ecosystem-disturbing plants.