• 식물분류학회지
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• 제42권2호
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• pp.167-170
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• 2012

희귀식물 분포연구의 일환으로 조사 과정 중 국내 분포가 명확하지 않고 극히 한정된 지역에 분포하는 양치식물 2종의 신분포지가 확인되었다. 함경도와 강원도에 분포하는 것으로 알려진 보태면마(Dryopteris coreano-montana)가 전남 장성군 입암산에서 확인되었으며, 제주도 남쪽에서 드물게 자라는 난대성 양치식물인 공작지네고사리(Dryopteris decipiens)가 전남 장흥군 천관산에서 발견되었다.

• 식물분류학회지
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• 제49권1호
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• pp.8-12
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• 2019

Although Myanmar is a biodiversity hotspot, little is known about its flora, particularly lycophytes and ferns, due to a lack of intensive inventories. In 2017, work on a total of 299 species of lycophytes and ferns found throughout the country, including 84 species from Natma Taung National Park in the Chin State, was published. However, a comparison of the flora of Myanmar with those of adjacent countries suggests that many more species are likely also to be distributed in this country. In the present study, two field surveys conducted in Natma Taung National Park during 2015 to make a checklist of lycophytes and ferns resulted in the discovery of three fern species not previously recorded in the flora of Myanmar. All occur in other countries in Southeast Asia as well as in other tropical areas. These species are Pteris bella (Pteridaceae), Dryopteris lepidopoda (Dryopteridaceae), and Loxogramme duclouxii (Polypodiaceae). The results will provide valuable information for the Myanmar fern checklist that is currently being prepared.

• 식물분류학회지
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• 제52권1호
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• pp.24-28
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• 2022

Pteris griffithii Hook., one of the rarest fern species on the Indian subcontinent, is reported from Bhutan for the first time. The identity of this species was confirmed through morphological determination at the National Herbarium (THIM) of the National Biodiversity Centre (NBC) of Bhutan. It was found only in one location, in Gyelpozhing in eastern Bhutan, at an elevation of 521 m a.s.l. on 10 January 2016. Given that a very limited study of this species was conducted, the knowledge baseline with regard to its distribution is poor. It is also reported that this species has not been found for several years. The species is also considered to be very rare or critically endangered in some countries; however, there are no assessments on the International Union for Conservation of Nature (IUCN) Red List for this particular species. This paper attempts to provide baseline information considering its rarity and data deficiency. This species is also reported from the adjacent neighboring Indian state of Arunachal Pradesh as very rare, and also from Myanmar; however, confirmation of its presence in China is not clear at this time. Therefore, considering its data deficient status, we attempt to document it scientifically to create a knowledgebase pertaining to this particular species. Concurrently, this species merits further research to understand its distribution patterns in Bhutan and any related anthropogenic threats.

• 한국식품영양과학회지
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• 제18권3호
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• pp.255-264
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• 1989

고사리의 ethanol extract를 독성실험한 결과는 mouse에 시료 10g/kg까지는 급성독$(LD_{50})$을 나타내지 않았다. Rat에 매일 시료40mg/kg, 400mg/kg, 1200mg/kg을 경구투여한 처음 7일간에는 체중, 해부소견, 혈액의 생화학적 검사에서 별이상이 없었다. 14일째 전시료투여군의 체중증가율은 대조군보다 평균 약 7% 감소를 보이고 혈청의 생화학적 측정치가 대부분 약간 증가하였다. 21일째 400mg/kg군과 1200mg/kg군의 체중증가율이 대조군보다 $6.7{\sim}5.7%$ 감소되고 1200mg/kg군 1필이 황색간증세를 보였다. 모든 시험군 GPT 및 GOT는 각각 $88{\sim}102u$와 $164{\sim}200u$로 증가하고 alkaline phosphatase도 상승하여 중독의 징후를 보였다. 28일째 400mg/kg군과 1200mg/kg군의 체중증가율이 대조군보다 감소되고 이들군에서 각각 $1{\sim}2$필이 황색간을 보였다. 1200mg/kg군 2필이 소장염을 보였다. 모든 시험군은 GPT $92{\sim}116u$와 GOT $56{\sim}215u$ 및 alkaline phosphatase $57{\sim}70u$로 증가 되었다.

• 생명과학회지
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• 제21권1호
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• pp.56-61
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• 2011

본 연구는 청나래고사리 성엽 추출물의 항산화물질의 함량과 항산화능을 향상시킬 수 있는 효과적인 추출방법을 개발하기 위하여 시행하였다. 동결건조한 청나래고사리 성엽의 분쇄시료 1 g을 메탄올, 80% 에탄올 및 물 등 3가지 용매와 섞은 후 6시간 동안 상온 침지, $60^{\circ}C$ 가열 및 200 rpm에서 교반하여 추출하거나 42 kHz의 초음파 수조에서 15, 30, 45분 동안 추출하였다. 추출물은 여과한 다음 가용성고형분, 총 폴리페놀 및 총 플라보노이드의 함량을 측정하였으며, 항산화활성은 DPPH와 ABTS radical 소거활성을 $RC_{50}$으로 환산하여 측정하였다. 80% 에탄올을 용매로 하여 30분 동안 초음파추출 하였을 때 가용성고형분(0.317 $g{\cdot}g^{-1}$ db), 총 폴리페놀(70.90 $mg{\cdot}g^{-1}$ db) 및 총 플라보노이드(41.53 $mg{\cdot}g^{-1}$ db)의 추출수율이 가장 우수하였으며, DPPH와 ABTS radical 소거활성 또한 가장 우수하였다(각 $RC_{50}$=0.14 $mg{\cdot}ml^{-1}$와 0.09 $mg{\cdot}ml^{-1}$). 상기의 연구결과에 따라 청나래고사리의 성엽은 천연항산화소재로 활용가능하며, 80% 에탄올을 용매로 15-30분 동안 초음파추출하는 것이 추출물의 항산화효과를 증가시키며 추출에 소요되는 시간을 줄일 수 있는 효과적인 추출방법으로 생각되었다.

• 생약학회지
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• 제16권3호
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• pp.155-159
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• 1985

Isomotiol $(fern-8-en-3{\beta}-ol)$ was isolated from the n-hexane fraction of Euphorbia supina Rafin. The structure of isomotiol was identified by IR, NMR, Mass spectra and compared with the authentic sample. Isomotiol was first isolated from the family of Euphorbiaceae.

• 한국자원식물학회지
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• 제32권6호
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• pp.735-742
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• 2019

This study investigated a suitable method that could be applied for Asian chain fern [Woodwardia japonica (L. f.) Sm.] to propagate gametophytes and promote sporophyte formation. The gametophytes used in all experiments were obtained from germinated spores in vitro and were subcultured at 8-week intervals. The most appropriate media for gametophyte propagation was identified by culturing 300 mg of gametophyte in Murashige and Skoog (MS) basal medium (1/8, 1/4, 1/2, 1, 2), and Knop medium for 8 weeks. As a result, fresh weight of the gametophyte was increased by 56.7-fold on MS medium. Moreover, antheridium formation as well as gametophyte growth was improved on MS medium, especially. To improve the sporophyte formation ex vitro, 1.0 g of gametophyte was ground with distilled water and spread on eight combinations onto four different culture mediums, such as bed soil, peat moss, perlite and decomposed granite. Then generation and growth of sporophytes were investigated after cultivation for 10 weeks. As a result of this experiment, peat moss had a promotive effect of sporophyte formation at single-use and mixed culture soils. In particular, a mixture of bed soil, peat moss and perlite in a 1:1:1 ratio (v/v/v) led to the accelerated formation (782.5 ea/pot) and the frond growth of sporophytes. This included increases in length and width of fronds. However, promotive effect of gametophyte growth and sporophyte formation was not found at single-use and treatment with high ratio of bed soil.

• The Plant Pathology Journal
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• 제22권1호
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• pp.51-62
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• 2006

Three strains of Cucumber mosaic virus (CMV) have been found to cause a lethal disease, referred to as fern leaf syndromes and mild mosaic symptoms in tomato (Lycopersicon esculentum Mill.) crops grown in Kuwait. CMV strains were detected and identified based on host range, symptomatology, serology, electron microscopy, and ribonucleic acid (RNA) electrophoresis in polyacrylamide gels. A high degree of viral genomic heterogeneity was detected among CMV strains isolated in Kuwait, with no apparent correlation to symptomatology in tomato host plants. Two different virus satellites of 'CMV associated RNA 5', designated CARNA 5, were detected in two virus strains that caused both lethal disease and mild symptoms, designated CMV-D1 and CMV-S1 respectively. CARNA5 was not detected in the third CMV strain that caused fern leaf syndromes designated CMV-F. All the three isolated strains were serologically indistinguishable from each other and may belong to one serotype according to Ouchterlony gel diffusion tests. These strains transmitted via aphids (Myzus persicae Sulz) in a non-persistent manner. Physical properties of the virus strains were very similar where thermal inactivation test showed that virus withstood heating for 10 min at $70^{/circ}$, dilution end point was $10^{-4}$, and the longevity in vitro at room temperature was less than 5 days for all virus strains. CMV-D1 and CMV-F were the most devastating diseases spreading in both greenhouse and field-grown tomato where aborted flower buds failed on fruit setting due to the viral infection. This is the first report to isolate three different strains of CMV in Kuwait.

• 한국지구과학회지
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• 제31권7호
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• pp.698-707
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• 2010

경기도 의왕시 포일동의 후기 플라이스토세 퇴적층에 대한 화분분석 결과, 초본류 및 양치식물로 이루어진 저층의 발달과 함께 침엽수-낙엽성 활엽수 혼효림이 분포했던 것으로 나타났다. 기후조건을 반영하는 식생조성의 변화가 인지되는데, 약 43,100년 전-41,900년 전 시기에는 오늘날보다 다소 추운 한랭한 온대 기후하에서 초지식생을 수반한 침엽수-낙엽성 활엽수 혼효림이 발달하였다. 약 41,900년 전-41,200년 전 동안에는 양치식물 저층의 발달과 함께 침엽수-낙엽성 활엽수 혼효림 내에서의 아한대성 침엽수의 감소와 온대성 낙엽 활엽수의 증가가 나타나는데 이는 기후조건의 온난화를 지시한다. 약 41,200년 전-39,700년 전 시기에는 기후 한랭화에 따른 아한대성 침엽수의 증가와 식생밀도의 감소가 나타난다.

• Journal of Applied Biological Chemistry
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• 제49권4호
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• pp.165-170
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• 2006

The antifungal activity of crude methanolic extract and fractions from Yucca smalliana Fern. leaves, roots and flowers were investigated in vitro against a panel of plant pathogenic fungi. The minimal inhibitory concentration(MIC) was determined by an agar dilution method. Preliminary liquid culture and agar plate assays showed that the growth of Fu sarium oxysporum, Phytophthora capsici, Rhizoctonia solani and Botrytis cinerea were inhibited by Y. smalliana extracts. The extracts from flowers and leaves showed antifungal activity of 64.0% and 34.0% against F. oxysporum, 66.0% and 62.0% against P. capsici, and 27.0% and 41.0% against B. cinerea, respectively. The methanolic extract from Y. smallina leaves in distilled water was fractionated using solvents of increasing polarity: hexane, ethyl acetate and butanol. These fractions had a broad spectrum of antifungal activity, found to reside entirely in the butanol and aqueous fraction. The aqueous fraction showed inhibition rate of 60.0, 67.8, 84.6 and 58.3% against F. oxysporum, R. solani, C. gloeosporioides, and B. cinerea, respectively, and the butganol fracgtion showed 36.0, 46.0, 66.1 and 58.3%, respectively. Phenolics(e.g. flavonoids, steroids and terpenoids) were observed in the thin layer profile of the different fractions. Leave extract showed a prominent antioxidant activity totally scavenging the free radical of DPPH at a concentration of 1 mg/ml.