• Asian-Australasian Journal of Animal Sciences
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• 제27권5호
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• pp.706-716
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• 2014

A new strategy of co-inoculating Bacillus subtilis MA139 with Streptococcus thermophilus and Saccharomyces cerevisiae was used to produce fermented soybean meal (FSBM). Three experiments were conducted to determine the concentration of digestible energy (DE) and metabolizable energy (ME) (Exp. 1), apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of amino acids (AA) (Exp. 2), and feeding value (Exp. 3) of FSBM produced by this new strategy (NFSB) compared with soybean meal (SBM) and conventionally available FSBM (Suprotein). In Exp. 1, twenty-four barrows (initial body weight [BW] of $32.2{\pm}1.7kg$) were randomly allotted to 1 of 4 diets with 6 replicates per diet. A corn basal diet and 3 diets based on a mixture of corn and 1 of 3 soybean products listed above were formulated and the DE and ME contents were determined by the difference method. The results showed that there were no differences in DE and ME between SBM and either FSBM product (p>0.05). In Exp. 2, eight barrows (initial BW of $26.8{\pm}1.5kg$) were fitted with ileal T-cannulaes and used in a replicated $4{\times}4$ Latin square design. Three corn-starch-based diets were formulated using each of the 3 soybean products as the sole source of AA. A nitrogen-free diet was also formulated to measure endogenous losses of AA. The results showed that the SID of all AA except arginine and histidine was similar for NFSB and SBM (p>0.05), but Suprotein had greater (p<0.05) SID of most AA except lysine, aspartate, glycine and proline than NFSB. In Exp. 3, a total of 144 piglets (initial BW of $8.8{\pm}1.2$ kg) were blocked by weight and fed 1 of 4 diets including a control diet with 24% SBM as well as diets containing 6% and 12% NFSB or 12% Suprotein added at the expense of SBM. During d 15 to 28, replacing SBM with 6% NFSB significantly improved average daily gain (ADG) and average daily feed intake (ADFI) (p<0.05) for nursery piglets. During the overall experiment, ADG of piglets fed diets containing 6% NFSB was significantly greater (p<0.05) than that of piglets fed SBM. In conclusion, fermentation with the new strategy did not affect the energy content or the AID and the SID of AA in SBM. However, inclusion of 6% NFSB in diets fed to nursery piglets improved performance after weaning likely as a result of better nutritional status and reduced immunological challenge.

• 한국식품저장유통학회지
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• 제24권1호
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• pp.145-152
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• 2017

본 연구에서는 전통 재래식 콩된장의 제조공정을 과학적으로 해석하기 위한 연구의 일환으로 생메주의 건조 및 발효과정에 있어서 수분함량과 단백질 분해에 의한 아미노산 생성과정을 근적외 분광법으로 쉽게 판단 할 수 있는지 그 가능성을 조사하였다. 생메주를 일정한 크기($3{\times}4{\times}3cm$)로 잘라서 수분이 30%가 되도록 $25^{\circ}C$ 항온기에 3주간 발효시킨 모델계 발효메주를 만들어 가시광-근적외 스펙트럼을 측정하여 해석하였다. 발효가 진행됨에 따라 수분이 감소되면서 수분과 관련이 있는 1,430 nm대의 흡광도가 감소 및 시프트 되는 것이 관찰되었고, 발효기간이 길어질수록 아미노산과 관련이 있는 2,050 nm대의 흡광도가 증가하는 것을 관찰 할 수 있었다. 전통 재래식 발효메주를 속부분과 겉부분으로 분리하여 1,000-2,250 nm에서의 가시광 스펙트럼과 근적외 스펙트럼 데이터로 판별분석한 결과, 겉부분과 속부분이 잘 판별되었고, 가시광 보다는 근적외 영역에서 잘 구분되었다. 이들 전통메주중의 아미노산 농도를 비파괴 측정하기 위해 PLSR한 결과 $R^2$은 0.9 이상이며, RMSECv 0.23%로 나타났으며, 전통메주중의 수분함량도 $R^2$ 0.81, RMSECv 0.83%이어서 근적외 분광법으로 전통메주의 품질을 비파괴 측정 가능할 것으로 판단되었다.

• 한국식품저장유통학회지
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• 제23권6호
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• pp.811-818
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• 2016

본 연구는 장류용 주요 콩 품종별, 유용발효미생물인 Bacillus subtilis HJ18-9 첨가 유무에 따른 청국장의 이화학적인 특징과 맛에 관여하는 아미노산 및 유기산 구성을 비교하고자 하였다. 원료콩 으로는 대원, 대풍, 새단백, 태광을 사용하였다. 원료콩(찐콩), starter를 첨가하지 않은 전통방법으로 제조한 청국장(청국장 c)과 starter를 첨가하여 제조한 청국장(청국장 t)의 수분함량은 62.45~67.12, 63.28~67.14, 64.50~66.87%이었으며, 아미노태 질소는 6.53~24.25, 27.63~122.09, 37.29~133.48 mg%, 암모니아태 질소는 26.92~47.95, 45.45~156.36, 28.02~121.13 mg%로 나타났다. 유리아미노산 함량은 원료콩이 청국장보다 감칠맛을 내는 glutamic acid, aspartic acid이 더 많은 반면, 쓴맛을 내는 valine, methionine, isoleucine, leucine, phenylalanine은 starter 첨가 유무에 관계없이 청국장이 원료콩보다 더 많은 양이 검출되었다. 유기산은 원료 콩에서 청국장으로 발효되면서 oxalic acid와 citric acid의 경우 그 함량이 감소하였고, malic acid, lactic acid, acetic acid, succinic acid는 그 함량이 증가하였다. SDS-page 확인 결과 원료콩에서는 넓은 분포로 band가 보였지만 청국장의 경우 분자량이 큰 단백질이 사라지고 작은 분자량이 증가하는 것을 확인할 수 있었다. 총 균수는 원료콩, 청국장 c 및 청국장 t의 경우, 각각 3.80~5.67, 8.14~8.85, 7.48~8.46 log CFU/mL의 범위로 나타났다. 이상의 결과로 콩품종에 따라 발효정도에 차이를 보이는 것과, starter첨가에 따른 품종별 발효양상이 다른 것을 확인하게 되었다. 특히, starter첨가 시, 다른 품종에 비해 새단백 청국장의 감칠맛과 연관된 glutamic acid와 aspartic acid의 함량이 증가하는 것으로 보아 주로 두부제조용으로 이용되었던 새단백의 용도다양화가 가능 할 것으로 판단된다. 본 내용은 콩품종 및 starter 첨가에 따른 청국장의 이화학적인 특징과 맛에 관여하는 아미노산 및 유기산 구성을 비교하여 청국장의 품질을 향상시키는데 필요한 기초자료로써 향후, 기능성 콩발효식품 및 콩품종 이용다양화에 활용 될 수 있을 것으로 기대된다.

• Journal of Applied Biological Chemistry
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• 제48권3호
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• pp.138-143
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• 2005

Antibacterial, free radical scavenging, proliferative, and cytotoxicity effects of Doenjang extracts were examined. All samples except water extract showed strong antibacterial activity against oral bacteria, Streptococcus. pyogenes, S. mutants, S. sanguinis, S. sorbrinus, S. criceti, S. antinosus, S. gordonii, and Porphyromonas. gingivalis (MIC and MBC values: 0.08-1.25 and 0.16-2.50 mg/ml, respectively). DPPH method showed ethanol and ethyl acetate extracts are effective inhibitors of oral bacteria. Based on MTT assay, 24 h exposure to 0.31 mg/ml of all extracts, excepted water extract, resulted in strong cytotoxicity on KB cells. All extracts strongly inhibited human gingival fibroblast viability.

• Mycobiology
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• 제40권4호
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• pp.258-262
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• 2012

cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces $H_2O_2$ over 7.0 nmol/min/mL using methyl glyoxal as a substrate. Use of rGLX as a generator of $H_2O_2$ improved the coupled reaction with recombinant manganese peroxidase resulting in decolorization of malachite green up to $150{\mu}M$ within 90 min.

• 한국식생활문화학회지
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• 제10권5호
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• pp.419-425
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• 1995

General views of 1,436 housewives respondents through the country on commercial kochujang (fermented hot pepper-soybean paste) were surveyed by questionnaires. The 65.0% of total respondents had experience of purchasing commercial kochujang on the market, mostly super market (43.0%) of respondents, and the respondents who has job and live in apartment purchased the commercial products more often than the others (80.5%). The reason for purchasing commercial products were convenience (36.1%) and short of time (13.0%). The critera for purchasing commercial product were previous experience (40.1%) and well known trademark (20.6%) and not for purchasing were concerning of noxiousness (29.2%) and inferior taste (25.0%). Respondents prefered glass packing (44.3%) and plastic bottle (20.2%) of 500 g pack for single usage (42.3%). The most important standards for kochujang taste were pungency (59.1%) and savory taste (28.6%). The problems indicated to commercial products were inferior taste (32.2%), especially too sweet (52.6%), and safety (20.6%).

• 한국독성학회:학술대회논문집
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• 한국독성학회 2001년도 International Symposium on Dietary and Medicinal Antimutgens and Anticarcinogens
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• pp.15-16
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• 2001

GCP is a novel functional food obtained from the extracts of soybean isoflavone fermented with basidiomycetes mycelia. The enzyme reaction makes GCP containing high concentration of isoflavone aglycons, particularly for genistein and high content of polysaccharides. GCP exerts its anti-tumor activities by anti-angiogenesis and induction of apoptosis in cancer tissues and by enhancement of immune responses in immune system.(omitted)

• 한국식품영양과학회지
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• 제28권2호
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• pp.458-463
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• 1999

Methanol extract and its fraction of traditional fermented soybean paste(doenjang) from Soonchang area were studied for growth suppression on the various tumor cells and suppression components, by using HPLC and GC were analysed. Hexane fraction of methanol extract was indicated 79%, 76%, 67%, 66%, 78% of growth suppression on L1210, P338D1, HepG2, WiDr and SNU 1 tumor cells, respectively. Ethylacetate fraction of methanol extract also showed 81%, 75%, 75%, 76% and 82% of growth suppression on the same tumor cells, respectively. Peak 8 obtained from HPLC of ethylacetate fraction indicated 81%, 77%, 77%, 75% and 79% of growth suppression on the same tumor cells and identified as a genistein, by comparing with standard one by HPLC analysis. Hexane fraction of methanol extract contained oleic acid, linoleic acid and palmitic acid.

• Food Science and Biotechnology
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• 제15권5호
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• pp.814-816
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• 2006

Monacolin K (MK) was produced on soybean using Monascus ruber GM011 by a two-stage-fermentation process. The optimal temperature was identified as $28^{\circ}C$. Higher yield was obtained by multiple-level-temperature cultivation than by single-level-temperature cultivation. The highest yield of total MK, 4.810 mg/g dry soybean product, was attained after 30 days of solid-state fermentation. No citrinin could be detected in the fermented soybean.

• 환경위생공학
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• 제1권1호
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• pp.31-40
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• 1986

It was observed by electron microscope (transmission electron microscope, Scanning electron microscope) as a study on microstructure of soybean after r-ray irradiation with the intensity of 5KGY, 7KGY, 10KGY and 15KGY, fermented with the named Bacillus subtilis SCF, which newly separated and identified. According to the progress fermentation, changes of soybean microstructure have been increased, especially irradiated soybeans more increased than non-irradiated them. Observation of microstructure by electron microscope showed that each protein body became more. expanded in the dimension and decomposed, spherosome around the protein body in unit area dispersed and dwindled in the numbers of it. As the fermentation on progress, changes of soybean microstructure were suitable on fermentation period of 7KGY soybean, 48-72hrs fermentation.