• 융합정보논문지
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• 제11권11호
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• pp.239-247
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• 2021

본 논문은 대체식품 및 화장품 원료로 사용되는 미강을 발효를 통해 기능성과 경쟁력을 증가시키는 것을 목표로 하였다. 미강 추출물과 누룩에서 분리된 Saccharomycopsis fibuligera A8로 발효된 미강 추출물을 이용해 세포독성과 항염효과를 확인하였다. 세포 독성의 경우 미강 추출물의 경우 100 ㎍/mL에서 세포독성을 보였으나 발효 미강 추출물의 경우에는 세포독성을 보이지 않았다. 한편 nitric oxide, IL-6, TNF-α, IL-1β와 같은 염증 지표를 통해 항염효과를 확인한 결과, 미강 추출물은 nitric oxide, TNF-α만 100 ㎍/mL이상의 농도에서 항염효과를 보였다. 발효 미강 추출물의 경우에는 각각 25 ㎍/mL, 25 ㎍/mL, 50 ㎍/mL, 50 ㎍/mL의 농도에서 항염효과를 보여 더 낮은 농도로 항염효과를 나타내는 것을 확인하였다. 한편 S. fibuligera A8 사균체의 세포 독성 및 항염 효과를 실험한 결과 발효 산물에서 사균체 역시 유효하게 작용하는 것을 알 수 있었다.

• 한국식품영양과학회지
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• 제31권6호
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• pp.1097-1101
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• 2002

복령 균사체의 항산화성 및 아질산염 소거작용을 알아보고자 복령균사체와 복령쌀을 열수와 에탄올로 추출하여 fish oil에 대한 지방산화도를 측정한 결과 대조구에 비하여 복령균사체 및 복령쌀 첨가구가 낮은 TBARS값을 나타내었다. 복령균사체 및 복령쌀 추출물은 합성항산화제인 BHA보다는 약간 높은 TBARS값을 나타내었으나, 저장 24시간째 BHA첨가구0.22MDA ppm에 비하여 복령균사체의 열수와 에탄올 추출물이 각각 0.43 MDA ppm,0.3,3 tfl)A ppm을 나타내어 BHA 첨가구와 유사한 값을 나타내었나. 복령균사체 및 쌀의 Fe$^{2+}$, Cu$^{2+}$ 대한 금속이온 봉쇄작용은 복령 쌀보다는 복령균사체에서 높은 활성이 나타났다. 전자공여능은 복령쌀에서는 25% 미만인 반면 복령균사체에서는 열수와 에탄을 추출물에서 각각 76.8, 79.9%의 높은 활성을 나타내었다. SOD유사활성은 모든 처리구에 서 25% 미만의 낮은 활성을 나타내었으며, BHA에서는SOD유사찰성이 나타나지 않았다. 아질산염의 소거작용은 열수추출보다는 에탄을 추출물에서 높게 나타났다. 복령균사체 에탄을 추출물이 가장 높은 아질산염 소거능을 나타내었다.

• Preventive Nutrition and Food Science
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• 제16권2호
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• pp.142-149
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• 2011

The purpose of this study was to elucidate the physicochemical and microbiological properties of Korean traditional rice wine, Makgeolli, supplemented with black garlic extract during fermentation. Black garlic extract was diluted with distilled water to produce 0.5% and 1.0% black garlic solutions. Those solutions were then used to make rice solutions which included 2 kg rice, 40 g Nuruk (a fermentation starter), and 14 g yeast. After being mixed, the rice solutions were fermented for 7 days in a water bath at $28^{\circ}C$. The alcohol contents of the control, 0.5% and 1.0% black garlic Makgeolli were 16.9, 16.0, and 16.2%, respectively. Total acidity, total soluble solids, and color increased throughout the fermentation process. There was an increase of microorganisms throughout the fermentation period in all the samples. Glucose was the highest free sugar, and succinic acid was the highest organic acid detected in all the samples. Thirty nine volatile compounds were detected in black garlic Makgeolli.

• 대한수의학회지
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• 제55권4호
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• pp.233-240
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• 2015

Although the effects of the rice bran have recently been investigated, there is no information regarding platelet physiology available. However, it is well known that fermented natural plants have a beneficial effect on cardiovascular diseases. Therefore, this study was conducted to investigate whether fermented rice bran extract (FRBE) with several plants (Artemisia princeps, Angelica Gigantis Radix, Cnidium officinale, and Camellia sinensis) affected agonist-induced platelet aggregation, and if so, what the underlying mechanism of its activity was. We performed several experiments, including in vitro platelet aggregation, intracellular calcium concentration and adenosine triphosphate release. In addition, the activation of integrin ${\alpha}_{II}b{\beta}3$ was determined using fibrinogen binding. Thrombus formation was also evaluated in vivo using an arterio-venous shunt model. The FRBE inhibited collagen-induced platelet aggregation in a concentration-dependent manner. FRBE significantly and dose dependently attenuated thrombus formation using rat arterio-venous shunt. FRBE suppressed the intracellular calcium mobilization in collagen-stimulated platelets. We also found that FRBE inhibited extracellular stimuli-responsive kinase 1/2, p38-mitogen-activated protein kinases and c-Jun N-terminal kinase phosphorylation. These results suggested that FRBE inhibited collagen-induced platelet aggregation, which was mediated by modulation of downstream signaling molecules. In conclusion, FRBE could be developed as a functional food against aberrant platelet activation-related cardiovascular diseases.

• Food Science and Biotechnology
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• 제15권2호
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• pp.238-243
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• 2006

The methanol extract of waxy brown rice fermented with Agrocybe cylindracea was prepared. The extract was then freeze dried and fed to rats at the level of 0.5, 1.0, 2.0 g/kg body weight for 14 days, followed by the treatment with carbon tetrachloride for three consecutive days to induce hepatotoxicity. After sacrificing the rats, the enzyme activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), and ${\gamma}$-glutamyl transpeptidase (${\gamma}$-GTP) in serum was determined. Biochemical analysis on serum for albumin, total protein, triglyceride, and total as well as HDL-cholesterol were carried out along with a histopathological study of liver tissues. Based on these data, we suggest that the waxy brown rice cultured with A. cylindracea may exert hepatoprotective activity against hepatotoxicity caused by chemicals such as carbon tetrachloride.

• 한국식품과학회지
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• 제38권6호
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• pp.779-784
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• 2006

기능성 강화를 목적으로 키토산 0.2%, 송이버섯 1.0%, 석류분말 1.2%, 산사추출분말 1.0%, 실크분말 1.2%, 은행잎추출분말 0.01%, 동아분말 1.2%, 마늘유 0.2%, 홍국분말 1.2%를 각각 첨가하여 고추장을 제조하였다. 각각의 소재가 고추장의 숙성에 미치는 영향을 조사한 결과 45일간의 숙성 기간별 pH, 산도, 포르몰태 질소함량, 색도의 변화는 소재 첨가구와 control 간에 큰 차이가 없었다. 항산화활성은 0.2%의 마늘유 첨가구가 control에 비해 약 2배 증가하였고 ACE저해활성은 1.2%의 실크분말 또는 0.01%의 은행추출분말 첨가 시 control에 비해 약 2배의 효과가 있었다.

• 생명과학회지
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• 제30권4호
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• pp.352-358
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• 2020

홍국은 한국에서 오랫동안 음식과 전통의학 소재로 사용되어 왔다. Monascus sp.의 생장과 2차 대사과정을 통해 Monascus 색소, monacolins, γ-aminobutyric acid 등을 생산할 수 있다. Monascus 종의 대사산물인 monacolin K, γ-aminobutyric acid과 dimerumic acid는 특정 항산화 효과로 인해 콜레스테롤과 혈압을 낮춘다고 알려져 있다. 이 연구는 Monascus sp.로 발효된 홍국쌀 에탄올 추출물의 항염증 활성을 조사하였다. 홍국 에탄올 추출물의 총 폴리페놀 함량을 측정하였고, 항염증 효과는 LPS가 유도된 Raw 264.7 cell에서 NO 생성, iNOS 및 사이클로옥시게나아제-2(cyclooxygenase-2 (COX-2)) 단백질 활성을 측정하였다. 총 폴리페놀 함량은 발효되지 않은 일반쌀보다 높은 함량을 나타내었다. NO 생성 저해활성 평가는 400 ㎍/ml 농도에서 LPS를 처리하지 않은 음성대조군의 NO 생성량과 유사한 결과를 나타내었다. iNOS 및 COX-2 단백질 발현 저해활성은 400 및 800 ㎍/ml에서 iNOS의 발현을 상당부분 억제하였다. 홍국 에탄올 추출물은 높은 항염증 효과를 확인하였으며, 기능성화장품 및 항염증소재로서 활용 가능할 것으로 사료된다.

• 한국의류산업학회지
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• 제15권4호
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• pp.635-641
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• 2013

This study examined the dye-properties of natural fabrics dyed with Coptis chinensis and Curcuma longa root fermented with fungi. The optimum culture conditions for the fermentation of microorganisms, the relationship between natural dye color and fermentation conditions were investigated. Two different medical herbs (ground to 80-100 mesh in size) were used as a natural dyeing source. Phellinus linteus (P. linteus), which can grow in different media, such as Agarmedium (only agar containing medium), maltose extract agar (MA) and potato dextrose extract agar (PDA) culture media, were isolated from the medium. P. linteus was confirmed to be the optimum microorganism for the fermentation of Coptis chinensis and Curcuma longa, and the MA medium was confirmed to be the best for culturing. When using the microorganism as the fermenting agent, $32^{\circ}C$ was found to be the optimum fermenting temperature for both natural colorants. Regarding the dyeing property of the fermented natural dye, silk was dyed quite darkly in an appearance by naked eye estimation and the K/S value in the color strength of silk reached a high level of 16 after the fermenting process. The washing fastness of dyed silk after treatment washing was reduced from 4 to under4 and indicates that dyed silk with fermented plant was not unsubstantial. The light fastness was 1 to 2, showing intended to maintain due to the fermentation process.

• Journal of Applied Biological Chemistry
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• 제59권2호
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• pp.149-154
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• 2016

An endophytic bacterial strain was isolated from kimchi, a Korean traditional fermented Brassica campestris and identified as Bacillus subtilis MJMP2 based on the 16S rRNA sequence. This strain showed strong antagonistic activity against Xanthomonas oryzae pv. oryzae (Xoo) KACC10331, the pathogen of bacterial rice blight disease, as well as activity against some other rice phytopathogenic fungi. The active compound was purified through size-exclusion chromatography and preparative High-performance liquid chromatography. The molecular weight was determined as m/z 1043 by mass spectroscopy, which is identical to that of iturin A. Furthermore, a crude extract from the culture supernatant of Bacillus subtilis MJMP2 showed inhibitory activity against rice blight disease in both a rice leaf explant assay and a pot assay. The crude extract also enhanced the length of roots of Arabidopsis thaliana. These results suggest that the strain Bacillus subtilis MJMP2 could be used as a biological agent to control rice blight disease.

• 동의생리병리학회지
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• 제30권5호
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• pp.301-307
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• 2016

The purpose of this study is to research the whitening effects of fermentation extract made from the Rice bran and Dendropanax(FRD) Fermentation conditions were as follows; 1) Dendropanaxand and Rice bran were blended in a ratio of 1 to 1, 2) a weight of sugar was 10% of the total weight, 3) an amount of enzyme was 0.1%, and 4) a temperature was 20℃. It has been fermented for 90 days. In order to observe the whitening effects of FRD, the author measured the cell viability and the inhibition rate of the melanin biosynthesis, the activity of tyrosinase and SOD (superoxide dismutase) in malignant melanoma, B16F10 cells. As a result, FRD significantly inhibited the cell viability of B16F10 in more than 500 ㎍/㎖. FRD significantly suppressed the generation of melanin, and that induced by α-melanocyte stimulating hormone (α-MSH) in more than 1,000 ㎍/㎖. FRD significantly decreased the activity of tyrosinase and that induced by α-melanocyte stimulating hormone (α-MSH) in more than 500 ㎍/㎖. FRD did not changed the activity of SOD in dose dependent manner. Therefore, the author considered that the fermentation extract made from a Rice bran and Dendropanax will be able to produce high value-added products, if used as a commercial. Therefore, the author considered that the fermentation extract made from a Rice bran and Dendropanax will be able to produce high value-added products, if used as a commercial.