• 한국식품과학회지
• /
• 제29권2호
• /
• pp.337-342
• /
• 1997

벌꿀발효주인 mead 와 melomel의 제조방법을 비교하기 위하여, 천연벌꿀을 희석하여 제조한 mead와 벌꿀로 삼투압추출법에 의해 얻은 매실쥬스를 희석하여 제조한 매실 melomel의 발효 중 알코올함량, 환원당, 가용성고형분, pH 및 총산도의 변화를 조사하였다. 전반적으로 발효 속도는 mead보다 melomel이 훨씬 빨랐다. 환원당과 가용성 고형분(Brix)은 발효시작 후 16일까지 점차적으로 감소하였으며, 이 기간 동안에 알코올함량은 점차적으로 증가하였다. mead의 경우는 초기 5일까지는 환원 당량의 감소속도와 알코올 생성속도가 다소 지연되는 양상을 나타냈다. 발효 21일 후에는 mead의 알코올함량은 7.6%에 이르렀으며, 매실 melomel은 12.4%에 도달하였다. pH와 총산도는 발효 초기의 변화 외에는 전 발효기간 중 큰 변화를 보이지 않았다. 발효가 끝난 mead와 매실 melomel은 미세여과를 통하여 광투과도가 99.4%인 밝고 투명한 제품을 얻을 수 있었다. 결론적으로 벌꿀을 사용하여 삼투압법으로 추출한 과실을 사용하여 벌꿀발효주를 만드는 melomel의 제조법이 단순히 벌꿀만을 사용하는 mead의 제조법에 비해 장점을 갖음을 알 수 있었다.

• Applied Biological Chemistry
• /
• 제27권3호
• /
• pp.139-145
• /
• 1984

수박은 소비자가 즐겨 먹는 신선한 과일로서 저장성이 낮아 손실향이 많을 뿐만 아니라 여름 한철만 소비자에게 공급할 수 있는 단점이 있어 이의 저장성 및 이용성을 높이고자 수박쥬스의 알콜발효를 연구하였다. 수박쥬스속에는 3.2%의 환원당이 함유되어 있었으며, Saccharomyces cerevisiae, Kluyveromyces fragilis와 Saccharomyces cerevisiae var. ellipsoideus 세균주를 사용하여 pH, 온도, 당 농도가 알콜발효에 미치는 영향을 조사한 결과, 수박쥬스에 glucose를 12% 첨가하고 pH 5.73 및 온도 $27^{\circ}C$에서 Saccharomyces cerevisiae var. ellipsoideus를 2% 접종시켰을 때, 발효 8일째 최대치인 5.3%의 알콜을 생성함을 알 수 있었다. 또한, 알콜 발효에 있어서 nitrogen sources와 여러 무기염류가 발효에 약간의 영향을 미친다는 사실을 알게 되었으며, 살균방법에 있어서는 $SO_2$ 처리$(K_2S_2O_5\;110ppm)$ 보다 고온 살균($121^{\circ}C$, 15분)하였을때 살균효과가 높을 뿐만 아니라 알콜 생성량도 높았다.

• 한국미생물·생명공학회지
• /
• 제15권6호
• /
• pp.408-413
• /
• 1987

토양으로부터 생 쌀보리 분해 효소 생산능이 있는 170주의 곰팡이와 4주의 방선균을 분리하여 이중 생 쌀보리 분해 효소 생산능이 가장 높은 No. 281 균주를 선정하여 동정한 결과 Rhizopus sp.로 밝혀졌으며, 밀기울을 기본 배지로 한 효소 생산 조건을 검토한 결과 효소 생산 최적 온도는 3$0^{\circ}C$, pH는 4.5-5.0 이고 배양 시간 5일 후에 최고의 역가를 보였다. 생쌀보리를 이용한 무증자 알코올 발효 실험에서는 분리한 Rhizopus sp. No. 281의 효소를 이용했을 때가 시판 효소보다도 에탄올 생성이 2% 증가된 결과를 나타내었다.

• 한국식생활문화학회지
• /
• 제11권4호
• /
• pp.497-506
• /
• 1996

Fermentation characteristics and quality changes of the typical Korean traditional alcoholic beverages (Kwahaju, Sokokju, Baikhaju, Samhaeju and Hosanchun) were investigated during fermentation and after aging, respectively. They were prepared by their own brewing methods with respect to the same contents of rice and Nuruk (Korean-style bran koji). The consumed total sugar content of the alcoholic beverages (Sokokju, Baikhaju, Samhaeju and Hosanchun) which were brewed with the multiple input steps of seed mash and raw material (ABMIS) was higher than that of the alcoholic beverage (Kwahaju) which was brewed with the single input step (ABSIS). The pH values of all the beverages were similar and were ranged from 3.2 to 3.6. The acidity of ABMIS were lower than that of ABSIS, but alcohol concentration of ABMIS were higher. The fermentation period of the alcoholic beverages with triple input steps was the longest among them. The inner temperature and microbial growth in all the beverages increased at each of input steps of the raw materials. Fusel oil content of ABMIS was lower than that of ABSIS. Major components of the fusel oil were iso-amyl alcohol, iso-butanol, n-propanol, ethyl acetate and acetaldehyde, and its minor components were methanol and n-butanol. The results on quality changes of the alcoholic beverages after aging showed increase of alcohol in ABMIS, but decrease of sugar and acidity. Fusel oil contents of Kwahaju and Samhaeju decreased to be ranged from 3.1% to 13.9% after aging, but those of Sokokju, Baikhaju and Hosanchun increased to be ranged from 10.9% to 23.0%. Sensory evaluation of ABMIS showed better scores than that of ABSIS. It was suggested that the brewing method with multiple input steps of seed mash and raw material was one of the methods to increase fermentation efficiency for brewing the Korean traditional alcoholic beverages.

• 한국식생활문화학회지
• /
• 제26권2호
• /
• pp.178-183
• /
• 2011

The objective of this study was to determine the quality and sensory characteristics of ginpi wine using different levels of ginpi at each fermentation step. In the first mash with or without ginpi, lactic acid bacteria and yeast counts were maximum between days 2 and 3 of fermentation. The reducing sugar content increased after 2 days of fermentation of the first mash. Brix content increased rapidly after 1 day of fermentation, but pH decreased rapidly. After 3 days of fermentation of the second mash, 0, 30, 60, or 90 g of ginpi were added, and the yeast counts were 8.89, 8.72, 8.81, and 8.88 \log CFU/mL, respectively but then continually decreased. After 3 days of ginpi wine fermentation of the second mash, the addition of 0, 30, 60, 90 g ginpi resulted in alcohol content of 11.40, 8.90, 9.40, and 8.95%, respectively, and after 3 days of fermentation, alcohol content increased slightly. The results of a sensory evaluation showed that overall acceptability of ginpi wine was not different with different levels of added ginpi. However, the flavor of the 90 g ginpi wine had the highest acceptability.

• 한국산업융합학회 논문집
• /
• 제25권4_2호
• /
• pp.595-602
• /
• 2022

This study was conducted to investigate the characteristics of apple fermentation when different types of salt were used during apple fermentation, and when salt was added or not. The pH decreased in all experimental groups during the fermentation process, and the final pH was 4.72-3.31. The sugar content was 17.8-18.5 °Brix, the lowest in the sea salt (FAS), and the highest in the control group (FA). The acidity was measured as 0.48-0.56%, which was high in the control group (FA), and was 0.83-1.06% on the 7th day of final fermentation. The alcohol content was generated from the 5th day of fermentation, and the control group (FA) showed a distribution of 39.23-29.4%, and the alcohol content was higher in the control group than in the experimental group, As for the total polyphenol content and total DPPH content, it was confirmed that the purified salt (3.11 mg GAE/mL) showed the highest total polyphenol content than the control (2.25 mg GAE/mL). When salt was added, the fermentation progress was excellent, and when the salt concentration was high, it was confirmed that the possibility of apple fermentation was high.

• KSBB Journal
• /
• 제23권3호
• /
• pp.213-218
• /
• 2008

일반적으로 약주 생산 공정에서 고정화 담체를 이용하는 연속식 반응기에서는 회분식 반응기와 비교하여 생성되는 알코을 생성량 못지않게 연속식 반응기에서 연속적으로 생성되고 있음을 알 수 있었다. 고정화 담체를 이용한 연속식 반응기는 약주의 품질에 긍정적인 효과를 보이는 것으로 나타났으며 담금 공정에서의 처리효과를 분석한 결과는 다음과 같다. 1) 회분식 배양에서 에탄올의 생성량은 1차 담금 1일 째부터 12%이상 증가하는 것으로 나타났으며 연속배양은 2차 담금에 효모를 주입하지 않아도 회분식 배양에서와 같은 알코올 도수를 가지면서 지속적으로 얻어졌다. 또한 pH는 4.4에서 3.8로 감소하는 패턴을 나타냈으며 산소농도는 1 ppm정도 차이가 나타났으나 이는 연속적으로 기질이 반응기로 주입되기 때문에 증가된 것이다. 2) 폴리플로필렌에 폴리스티렌을 샌드위치식으로 접합한 고정화 미생물담체는 연속배양에서 효모 주입 없이도 회분식 배양과 비교해보았을 경우에 당화과정과 알코올 생성과정 등이 서로 상반되는 패턴을 갖으면서 생물학적인 반응이 정상적으로 진행됨을 증명하였다. 3) 연속배양의 실험결론을 토대로 1차 담금에서 당의 생성기질을 이용하였을 경우, 10톤/일 생산하는 약주생산 공장은 1년간 효모 투입비용을 환산해 본 결과 1억원 이상의 투입비용이 감소하는 것으로 나타났다.

• 한국미생물·생명공학회지
• /
• 제37권4호
• /
• pp.413-415
• /
• 2009

To develop a new traditional rice wine by using Gardenia jasminoides, various fermentation methods such as without cooking, with cooking and with starter seed methods were studied. The condition of alcohol fermentation was investigated by addition of 1% Gardenia jasminoides into mash. Among the fermented methods, the fermentation with starter seed was the best as the alcohol was 19%. The acceptability of the Gardenia jasminoides rice wine with different methods were compared. The starter seed method which was prepared by adding 1% Gardenia jasminoides into mash showed the best acceptability in the sensory evaluation test and color test.

• Journal of Ginseng Research
• /
• 제3권2호
• /
• pp.95-104
• /
• 1979

The effects of ginseng extracts on carbon dioxide generation, alcohol fermentation, and yeast cell production by Saccharomyces cerevisiae were investigated. The results are as follows. 1) In the process of fermentation, CO2 generation by yeast is faster in ginseng extracts media of 0.3%, 0. l% than in control. As the concentration of the extracts increases by 0.7% and 1.5%, CO2 generation is decreased. Among all these concentrations, CO2 generation is fastest in 0.3% of the extracts. 2) In the process of fermentation, the production of alcohol is larger in the order of 0.3%, 0.7% and 0.1% than in the control and least in 1.5%. 3) The number of yeast cell rapidly increased from 12 hours to 18 hours after cultivation and conspicuously increased in the order of 0.3%, 0.7%, 0.1%, control and 1.5%. 4) Dried yeast cell weight increased more in all the above concentration than control and among these it increased visibly in 0.3% of the extracts.

• Applied Biological Chemistry
• /
• 제27권1호
• /
• pp.52-54
• /
• 1984

Ethanol fermentation from the chemically gelatinized starchy material was examined. The critical concentration of sodium hydroxide solution for gelatinization was dependent on the species of starch; 0.4M for potato and 0.6M for tapioca at room temperature. For alcohol fermention the starchy material was gelatinized by addition of sodium hydroxide solution, neutralized by sulfuric acid, and then yeast was added. The amount of $CO_2$ evolved during ethanol fermentation indicates that non-fermentable material was not produced from the starch by chemical gelatinization. In ethanol fermentation of potato and tapioca starch no significant difference was observed between the thermal and the chemical gelatinization.