• Korean Journal of Food Science and Technology
• /
• v.48 no.1
• /
• pp.48-53
• /
• 2016

The objective of this study was to evaluate the accuracy and efficiency of different methods used for the fermentable sugar assay in the production of Makgeolli sul-dut. In the initial stage of fermentation, Ipguk treatment produced a higher alcohol content compared to the Nuruk treatment. However, the alcohol content was not significantly different between the two starters at the final stage of fermentation. Acidity in the Ipguk treatment was higher than that of Nuruk throughout the fermentation period. After analyzing the fermentable sugars using dinitrosalicylic acid (DNS), Fehling's method, refractometer, glucose kit, and high performance liquid chromatography (HPLC), it was confirmed that the HPLC method was the most accurate for fermentable sugar quantification. In both types of starters, DNS and Fehling's methods showed results comparable to HPLC in terms of fermentable sugar content, while the glucose kit and refractometer analyses showed relatively large discrepancies, indicating that the Fehling's method could also be effective for the analysis of fermentable sugars in the manufacture of Makgeolli.

• Applied Biological Chemistry
• /
• v.34 no.2
• /
• pp.180-186
• /
• 1991

A water soluble antifungal antibiotic, Solumycin, was separated from the culture broth of Streptomyces sp. LAM-593, isolated from soil, by butanol extraction, alumina-, 1st and 2nd Sephadex LH-20 column chromatography. The substance was pale yellow crystal which gave a single spot at Rf value 0.24 with ethanol-ammonia water-water (8:1:1), 0.46 with butanol-ethanol-water (5:1:4), 0.84 with 50% methanol on silica gel TLC. It was dissolved well in water, methanol and acidic aq. butanol but not in ethanol, acetone, ethyl acetate, chloroform. acetic acid etc., and gave positive Fehling and Molish reaction. The UV spectrum in methanol showed absorption at 342, 361, 380, and 404 nm. The antibiotic was active against fungi such as Candide, Cryptococcus, Saccharomyces, Trichophyton and Trichosporon, but not to bacteria such as Bacillus, Escherichia and Staphylococcus.

• Steel and Composite Structures
• /
• v.23 no.6
• /
• pp.669-682
• /
• 2017

The use of ultra high performance concrete (UHPC) in composite columns offers numerous structural benefits, and has received recent research attention. However, the information regarding the behavior of steel tube confined concrete (STCC) columns employing UHPC has been extremely limited. Thus, this paper presents an overview of previous experimental studies on circular STCC columns with taking into account various concrete strengths to point out their distinctive features. The effect of the confinement factor and the diameter to thickness ratio on both strength and ductility in circular STCC columns employing UHPC was investigated. The applicability of current design codes such as EC4, AISC, AIJ and some available analytical models for concrete confined by steel tube was also validated by the comparison of ultimate loads between the prediction and the test results of Schneider (2006) and Xiong (2012). To predict the stress-strain curves for confined UHPC in circular STCC stub columns, a simplified model was proposed and verified by the comparison with experimental stress-strain curves.

• Korean Journal of Environmental Agriculture
• /
• v.16 no.1
• /
• pp.1-6
• /
• 1997

This study was carried out to identify the three antifungal substances isolated from the culture medium of Pseudomonas sp. A-183 which is antagonistic against Phytophthora capsici. The substance A and B showed positive reactions at the Molish test and Anthrone test, but negative one at the Fehling test, strongly suggesting that both substance A and B had nonreducing sugar frameworks. The substance C only exhibited the phenomenon of the UV induced fluorescence. From the qualitative analysis with the spectroscopic techniques such as UV, Mass, IR and NMR, the substance A and B were known to be composed to sugar and fatty acid, and showed a base peak of 171(m/e). It was identified that substance A was $(2-O-L-rhamnosyl-{\alpha}-L-rhamnosyl-{\beta}-hydroxydecanoyl-{\beta}-hydroxy$ decanoic acid) and the substance B was $({\alpha}-L-rhamnosyl-{\beta}-hydroxydecanoyl-{\beta}-hydroxy$ decanoic acid). The substance C was identified as a phenazine from the results of qualitative analysis with the spectroscopic techniques such as UV, Mass, IR and NMR.

• Journal of Korean Society of Forest Science
• /
• v.35 no.1
• /
• pp.24-32
• /
• 1977

According to the chlorous salt method, most of holocellulose whose lignin was removed, was obtained. In extracting xylan from holocellulose by the different densities of alkali, 5% KOH was extracted three times but still there remained part of xylan in it and another composite of hemicellulose and cellulose was obtained. The extraction of 10% and 20% KOH showed a desirable result. Rather than the ordinary method to use a large quantity of ethanol in the precipitation isolation of xylan, the method to use a small quantity of ethanol in adopting the dialysis with cellophane-membrane by condensing density to one tenth, made il possible to extract a high purity xylan in a high retrieving rate. In isolating glucomannan, the residue of 5% KOH extraction contained a large quantity of xylan, the residue of 10% and 24% KOH extraction, also showed the same result and the comparison between glucose and mannose was approximately 1 : 1. The purification of Fehling solution made it possible to obtain comparatively pure xylan but the process of oxidation dissolution was complicated and the retrieving rate was low. This was not a good method. The ethanol titration purified a high purity xylan in a high retrieving rate and was a very excellent purifying method, considering its simple and easy process. These two purifying methods, however, could not completely remove the residue of arabinose. This will be examined and reported later.

• Structural Engineering and Mechanics
• /
• v.63 no.3
• /
• pp.371-384
• /
• 2017

Ultra high performance concrete (UHPC) has recently been applied as an alternative to conventional concrete in construction due to its extremely high compressive and tensile strength, and enhanced durability. However, up to date, there has been insufficient information regarding the confinement behavior of UHPC columns. Therefore, this study aims to perform an assessment of axial stress-strain model for UHPC confined by circular steel tube stub columns. The equations for calculating the confined peak stress and its corresponding strain of confined concrete in existing models suggested by Johansson (2002), Sakino et al. (2004), Han et al. (2005), Hatzigeorgiou (2008) were modified based on the regression analysis of test results in Schneider (2006) in order to increase the prediction accuracy for the case of confined UHPC. Furthermore, a new axial stress-strain model for confined UHPC was developed. To examine the suitability of the modified models and the proposed model for confined UHPC, axial stress-strain curves derived from the proposed models were compared with those obtained from previous test results. After validating the proposed model, an extensive parametric study was undertaken to investigate the effects of diameter-to-thickness ratio, steel yield strength and concrete compressive strength on the complete axial stress-strain curves, the strength and strain enhancement of UHPC confined by circular steel tube stub columns.

• Steel and Composite Structures
• /
• v.29 no.1
• /
• pp.125-138
• /
• 2018

The research on the confinement behavior of ultra high performance concrete without and with the use of steel fibers (UHPC and UHPFRC) has been extremely limited. In previous studies, authors experimentally investigated the axially compressive behavior of circular steel tube confined concrete (STCC) short and intermediate columns with the employment of UHPC and UHPFRC. Under loading on only the concrete core, the confinement effect induced by the steel tube was shown to significantly enhance the utimate stress and its corresponding strain of the concrete core. Therefore, this paper develops a simplified stress - strain model for circular STCC columns using UHPC and UHPFRC with compressive strength ranging between 150 MPa and 200 MPa. Based on the regression analysis of previous test results, formulae for predicting peak confined stress and its corresponding strain are proposed. These proposed formulae are subsequently compared against some previous empirical formulae available in the literature to assess their accuracy. Finally, the simplified stress - strain model is verified by comparison with the test results.

• Structural Engineering and Mechanics
• /
• v.62 no.4
• /
• pp.417-430
• /
• 2017

Ultra high performance concrete (UHPC) has aroused interest around the world owing to superior mechanical and durability properties over conventional concrete. However, the application of UHPC in practice poses difficulties due to its inherent brittleness. UHPC filled in steel tube columns (UHPC-FSTCs) are capable of restricting the brittle failure of non-reinforced UHPC columns and forming a high performance member with enhancement of strength and ductility. Currently, research on UHPC-FSTCs remains very limited and there is relatively little information about the mechanical behavior of these columns. Therefore, this study presents a review of past experimental studies to have a deeper insight into the compressive behavior of UHPC-FSTCs under axial loading on entire section and on concrete core. Based on the test results obtained from Schneider (2006) and Xiong (2012), an analysis was conducted to investigate the influence of the confinement index (${\xi}$) and diameter to steel tube thickness ratio (D/t) on the strength and the ductility in short circular UHPC-FSTCs. Furthermore, the appropriateness of current design codes including EC4, AISC, AIJ and previous analytical models for estimating the ultimate loads of composite columns was also examined by the comparison between the predictions and the test results. Finally, simplified formulae for predicting the ultimate loads in two types of loading pattern were proposed and verified.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.14 no.1
• /
• pp.57-60
• /
• 1985

The inhibitory substance on the growth of mung bean seedling was amorphous crystal that had a single spot on silica gel thin layer chromatography under ultra-violet irradiation in dark room. The Rf value was 0.85 in n-butanol-acetic acid-formic acid-water(15 : 12 : 3 : 10) system, and 0.92 in n-butanol-acetic acid-water(4 : 1 : 5) system, respectively. Biochemical reaction of the inhibitor was negative to $FeCl_3$, pyrimidine, ninhydrine, folin, fehling, Salkowski and Ehrlich reaction, but the inhibitor had a blue fluorescence from irradiation of ultra-violet rays and had maximum absorption at 252nm on UV spectrum. On IR spectrum, the Peaks of the inhibitor appeared at $3300{\sim}3500,\;2900{\sim}3000,\;1600{\sim}1700,\;and\;1400cm^{(-1)}$. The strain tested was identified to be similar Streptomyces iuteogriseus by morphorogical and physiological characteristics.