• Environmental Analysis Health and Toxicology
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• 제5권1_2호
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• pp.37-44
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• 1990

Synthetic detergents and surfactants are in widespread usage as household and industrial detergents. Potential human toxic hazard arises following percutaneous absorption or oral ingestion of solution residues from kitchen and feeding utensils, fruits, and vegetables and contaminated water supplies. A toxicological investigations was performed with the synthetic detergents and surfactants [linear alkyl benzene sulfonate (LAS), ${\alpha}$-olefin sulfonate (AOS), sodium lauryl sulfonate (SLS), sodium lauryl ester sulfonate (SLES)］, In acute toxicity, agents were administered subcutaneously into ICR mice. In acute study, after lowering of spontaneous motility, respiratory failure, death appeared, vomitting was often associated with salivation and or retching. No sex difference was observed in LD$\sub$50/ of mice. In subacute toxicity, agents were administered orally into SD rats. Body weight increase was suppressed and there was no adverse effect on food and water consumption. The weight of organs were not changed by agents as compared with control group. No specific change was observed in biochemical and hematologicalor data.

• 농약과학회지
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• 제17권1호
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• pp.33-40
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• 2013

온실가루이와 담배가루이의 살충과 행동에 미치는 pyrifluquinazon의 효과를 조사하였다. Pyrifluquinazon은 가루이류의 성충에 대해 50 ppm부터 12.5 ppm까지 90% 이상의 살충활성을 나타내었으나, 알과 약충에는 효과가 없었다. 뿌리와 잎을 통한 침투이행효과는 없었으나, 잔효성 검정에서 처리 후 5일차까지 90% 이상의 살충활성을 나타내었고, 90% 이상의 방제효과를 나타내었다. 접촉독성과 섭식독성에 의한 가루이류의 행동과 발현속도를 조사한 결과, 접촉 1시간 이후부터 가루이류는 바닥에 떨어져 심한 날개경련과 마비를 일으키는 증상을 나타내었으며, 섭식 3시간 이후부터 유사한 증상을 나타내었다. 한편, 선택시험에서 가루이류는 방사 1시간 이후부터 기피행동을 나타내었다. 따라서 pyrifluquinazon는 온실가루이와 담배가루이에 속효적으로 작용하고 날개 경련과 마비증상을 유발하여 식물체로부터 바닥으로 떨어져 아사하는 효과를 나타내지만, 직접적인 섭식억제효과는 없는 것으로 여겨졌다.

• Nutrition Research and Practice
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• 제7권2호
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• pp.109-114
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• 2013

We compared the preventive capacity of high intakes of vitamin C (VC) and vitamin E (VE) on oxidative stress and liver toxicity in rats fed a low-fat ethanol diet. Thirty-two Wistar rats received the low fat (10% of total calories) Lieber-DeCarli liquid diet as follows: either ethanol alone (Alc group, 36% of total calories) or ethanol in combination with VC (Alc + VC group, 40 mg VC/100 g body weight) or VE (Alc + VE group, 0.8 mg VE/100 g body weight). Control rats were pair-fed a liquid diet with the Alc group. Ethanol administration induced a modest increase in alanine aminotransferase (ALT), aspartate aminotransferase (AST), conjugated dienes (CD), and triglycerides but decreased total radical-trapping antioxidant potential (TRAP) in plasma. VE supplementation to alcohol-fed rats restored the plasma levels of AST, CD, and TRAP to control levels. However, VC supplementation did not significantly influence plasma ALT, AST, or CD. In addition, a significant increase in plasma aminothiols such as homocysteine and cysteine was observed in the Alc group, but cysteinylglycine and glutathione (GSH) did not change by ethanol feeding. Supplementing alcohol-fed rats with VC increased plasma GSH and hepatic S-adenosylmethionine, but plasma levels of aminothiols, except GSH, were not influenced by either VC or VE supplementation in ethanol-fed rats. These results indicate that a low-fat ethanol diet induces oxidative stress and consequent liver toxicity similar to a high-fat ethanol diet and that VE supplementation has a protective effect on ethanol-induced oxidative stress and liver toxicity.

• 한국미생물·생명공학회지
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• 제18권1호
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• pp.18-25
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• 1990

우리나라에서 생산되고 있는 패류 중 비교적 PSP의 검출률과 독소함량이 높은 것으로 알려진 진주담치를 시료로 하여 PSP로 인한 식중독사고의 예방과 독화된 패류의 활용방안을 모색하기 위하여 독화된 진주담치의 여러가지 처리조건 즉, 동결과 해동, 가열, 향신료 첨가 등이 독력에 미치는 영향과 통조림 제조공정 중의 독력변화에 관하여 실험한 결과를 요약하면 다음과 같다. 1. PSP는 동결과 해동에 의하여 전혀 영향을 받지 않을 뿐만 아니라 마늘과 생강액즙의 첨가에 의해서도 영향을 받지 않는다. 2. PSP를 가열하였을 때 $70^{\circ}C$ 이하에서는 60분간의 가열에 의해서도 거의 독력변화가 없었으나, $80^{\circ}C$ 이상에서는 온도의 상승에 따라 독력의 감소율도 증가하였다. 3. 독화된 진주담치를 통조림 제조하였을 때 살균공정에서 독력의 감소가 심하여 최초 독력의 90이상이 감소하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제12권4호
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• pp.642-650
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• 1999

Options available for utilization of animal wastes include sources of plant nutrients, feed ingredients for farm animals, substrate for methane generation, and substrate for microbial and insect protein synthesis. The wastes have the most economic value for use as animal feed. Performance of animals fed diets containing animal wastes is similar to that of animals fed conventional diets. Processing of animal wastes to be used as animal feed is necessary for destruction of pathogens, improvement of handling and storage characteristics, and maintenance or enhancement of palatability. Feeding of animal waste has not adversely affected the quality and taste of animal products. In the USA copper toxicity has been reported in sheep fed high-copper poultry litter, but this is not a serious problem with cattle. Potential pathogenic microorganisms in animal wastes are destroyed by processing such as heat treatment, ensiling and deep stacking. Incidents of botulism, caused by Clostridium botulinum, have been reported in cattle in some countries, and this problem was caused by the presence of poultry carcasses in litter. This problem has not occurred in the USA. With appropriate withdrawal, heavy metal, pesticide or medicinal drug accumulation in edible tissues of animals fed animal wastes is not a problem. Feeding of animal wastes is regulated by individual states in the USA. The practice is regulated in Canada, also. With good management, animal wastes can be used safely as animal feed.

• 한국환경보건학회:학술대회논문집
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• 한국환경보건학회 2005년도 국제학술대회
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• pp.73-83
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• 2005

Higher methylmercury (MeHg) accumulation and susceptibility to toxicity in the fetus than in the mother at parturition is well known. However, the difference in MeHg exposure to fetus and offspring throughout gestation and suckling is not well established. In the human, the effects of MeHg exposure on pregnant and breast-feeding women remain an important issue for elucidation, especially those of continuous uptake in high-fish-consumption populations. The purpose of this paper was to evaluate the difference in MeHg exposure to fetus and offspring throughout gestation and lactation using our recent animal and human studies data. In the animal study, adult female rats were given a diet containing 5 ${\mu}$g/g Hg (as MeHg) for 8 weeks. Then they were mated and subsequently given the same diet throughout gestation and suckling. On embryonic days 18, 20, 22 and at parturition, the concentrations of Hg in the brains of fetus were approximately 1.5-2.0 times higher than those in the mothers. However, during the suckling period Hg concentrations in the brain rapidly declined to about 1/10 of that during late pregnancy. Hg concentrations in blood also decreased rapidly after birth. In human study, Hg concentrations in red blood cells (RBCs-Hg) in 16 pairs of maternal and umbilical cord blood samples were compared at birth and 3 months of age after parturition. RBCs-Hg concentration in the umbilical cords was about 1.6 times higher than those in the mothers at parturition. However, all the infants showed declines in Hg concentrations throughout the breast-feeding period. The Hg concentration in RBCs-Hg at 3 months of age was about half that at birth. Both the animal and human studies indicated that MeHg exposure to the fetus might be especially high but it dramatically decreases during the suckling period. Therefore, close attention should be paid to the gestation rather than the breast-feeding period to avoid the risk of MeHg to human infants.

• Clinical and Experimental Pediatrics
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• 제49권1호
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• pp.6-13
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• 2006

Jaundice is one of the most common gastrointestinal conditions found in neonatal period, and most jaundice is benign. But because of the possibility of bilirubin toxicity, every newborn infants must be examined to identify the development of severe hyperbilirubinemia. To prevent the development of severe hyperbilirubinemia, promote and support successful breast-feeding, perform a systemic assessment before discharge for the risk of severe hyperbilirubinemia, provide early and close follow-up program, and treat with phototherapy or exchange transfusion or other therapeutic modalities, if indicated, are recommended.

• 한국식품영양과학회지
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• 제30권6호
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• pp.1246-1252
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• 2001

백김치의 생체내 Am독성억제효과를 확인하기 위하여 Am 식이에 10% 백김치(동결 건조분말) 첨가사료로 사육한 흰쥐 의 성장회복능을 실험한 결과를 요약하면 다음과 같다. Am 식이군의 섭취량이 양적으로 다른 군에 비해 적었으며, 기본 식이군 생배추식이군, 백김치식이군은 거의 같은 섭취량을 보였다. 기본식이군의 체중이 사육종료일에 289.4 g에 달하는데 비해 Am식이군은 229.8 g으로 기본식이군의 79.4%에 불과해 체중증가 현상이 크게 저조하였다. 그러나 백김치를 첨가한 식이군의 경우는 설사증상이 없어지고 체중도 기본 식이군의 경우와 비슷한 수준까지 회복상태를 보여 백김치의 Am에 대한 독성억제효과를 나타내었다. 변의 총배설량은 백김치식이군(1737.99 g/3 weeks)이 기본식이군의 약 15배, 생배추식이군의 약 1.6배로 백김치가 변부피를 증가시키는 데 가장 효과적인 것으로 확인되었다. 대장과 맹장의 무게는 생배추 및 백김치식이군에서 유의적 (P<0.05)인 증가를 나타내었다. 또한 신장의 무게는 Am식이군에서 유의적인 감소를 나타내어 Am의 독성이 신장과 깊은 상관성이 있음을 시사하고 있었다. Am식이군의 단백질(50.26%) 및 지질소화율 (58.37%)이 백김치식이군의 단백질(77.30%)및 지질소화율 (92.12%)에 비하여 매우 낮았는데, 이것은 이들 영양소의 낮은 소화율이 횐쥐의 체중저하에 직접적인 영향을 미친 것으로 생각되었다. 백김치의 수용성 및 불용성식이섬유는 소화관을 통과한 형태 변화가 뚜렷하게 관찰되었다. 변의 배설 형태는 백김치식이군의 변이 가장 부피가 크고 일정한 크기를 보였다.

• 한국해양바이오학회지
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• 제11권2호
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• pp.52-61
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• 2019

Over the past few decades, microalgae-based biotechnology conjugated with innovative CRISPR/Cas9-mediated genetic engineering has been attracted much attention for the cost-effective and eco-friendly value-added compounds production. However, the discharge of reproducible living modified organism (LMO) into environmental condition potentially causes serious problem in aquatic environment, and thus it is essential to assess potential environmental risk for human health. Accordingly, in this study, we monitored discharged genetically modified microalgae (GMM) near the research complex which is located in Daejeon, South Korea. After testing samples obtained from 6 points of near streams, several green-colored microalgal colonies were detected under hygromicin-containing agar plate. By identification of selection marker genes, the GMM was not detected from all the samples. For the lab-scale environmental risk assessment of GMM, acute toxicity test using rotifer Brachionus calcyflorus was performed by feeding GMM. After feeding, there was no significant difference in mortality between WT and transformant Chlamydomonas reinhardtii. According to further analysis of horizontal transfer of green fluorescence protein (GFP)-coding gene after 24 h of incubation in synthetic freshwater, we concluded that the GFP-expressed gene not transferred into predator. However, further risk assessments and construction of standard methods including prolonged toxicity test are required for the accurate ecological risk assessment.

• 농업과학연구
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• 제43권2호
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• pp.205-214
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• 2016

A drought-tolerant transgenic rice (Agb0103) was developed using a pepper methionine sulfoxide reductase (CaMsrB2) under the control of rice Rab21 promoter with a selection marker, the phosphinothricin acetyltransferase (PAT) gene. Commercialization of genetically modified (GM) crops will require the evaluation of risks associated with the release of GM crops. With the potential problems associated to GM crops safety testing, the investigation of their effects on non-target organisms is necessary for environmental risk research. This study was carried out to assess acute toxicity of a GM crop using the water flea (Daphnia magna) for non-target organism risk evaluation. The effect of acute toxicity on Daphnia magna of Agb0103 rice and a non-GM rice, Ilmibyeo, were investigated at different concentrations (0, 625, 1,250, 2,500, 5,000, and 10,000 mg/L). The Agb0103 rice used for the test was confirmed to express the CaMsrB2/PAT gene by the PCR and ELISA. Daphnia magna feeding tests showed no significant differences in cumulative immobility or abnormal response with either Agb0103 rice or non-GM rice. The 48hr-EC50 values showed no difference between Agb0103 rice (2243 mg/L) and non-GM rice (2694 mg/L). These results suggest that there is no significant difference in toxicity to Daphnia magna between Agb0103 rice and its non-GM counterpart.