• Asian-Australasian Journal of Animal Sciences
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• 제14권11호
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• pp.1634-1637
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• 2001

Allantoin and uric acid were estimated in milk to study the association between the levels of these purine derivatives and milk production per day under given feeding regimens. Keeping the stage of lactation, parity and initial milk yield in view thirty lactating buffaloes were randomly selected from early lactating group. All the animals were fed 30 kg green, 2 kg straw and 5 kg concentrate mixture on per animal/day basis at basal level up to 8 1 produce. 1 kg concentrate mixture, soaked cotton seed and boiled cotton seed was fed for every 2 I milk, respectively in Group I (control), Group II and Group III animals. Average milk Allantoin and Uric acid levels were $120{\pm}11.7g/ml$ and $4.03{\pm}0.63g/ml$, respectively in milk. Cotton seed feeding enhanced the milk production significantly (p<0.01) in comparison to concentrate mixture fed control group animals. A significant difference (p<0.01) in milk allantoin levels was found over the different feeding management at higher level of production group animals. Study also revealed a significant negative correlation between the milk allantoin and production per day r=-0.43 (p<0.05).

• Fisheries and Aquatic Sciences
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• 제13권2호
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• pp.118-126
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• 2010

A feeding trial was conducted to investigate the effects of different levels of dietary n-3 highly unsaturated fatty acids (HUFA) (1.1-5.6%) and vitamin E (70 and 400 mg/kg) on the growth and body composition of juvenile rockfish. Six isonitrogenous (45% crude protein) and isolipidic (17% crude lipid) diets were formulated to contain graded levels of n-3 HUFA and vitamin E. Diets 1, 2 and 3 consist of 400 mg vitamin E/kg diet with graded levels of 1.1, 3.0, and 5.6% n-3 HUFA, respectively. Graded levels of n-3 HUFA (1.1, 3.0, and 4.0%) were added in diets 4, 5 and 6, respectively, containing 70 mg vitamin E/kg diet each. At the end of feeding trial, growth performance of rockfish was affected by neither dietary n-3 HUFA nor vitamin E levels. Feed efficiency and hepatosomatic index were slightly decreased (P<0.05) with increment of dietary n-3 HUFA at each dietary vitamin E level. Dietary vitamin E and n-3 HUFA levels did not affect proximate composition and vitamin E concentration in the dorsal muscle of rockfish. Liver moisture and crude protein contents positively related to dietary n-3 HUFA levels. Liver lipid content and hematocrit value were significantly decreased (P<0.05) by increasing dietary n-3 HUFA levels. Eicosapentaenoic acid (20:5n-3; EPA) and docosahexaenoic acid (22:6n-3; DHA) concentrations in the dorsal muscle significantly correlated to dietary n-3 HUFA levels, except for fish fed the diet 6 containing 4% n-3 HUFA and 70 mg vitamin E/kg diet. EPA concentration in the dorsal muscle of fish fed the diet 6 was significantly lower than that of fish fed the diets 2, 3 and 5. The present findings suggest that feeding of diets containing excessive n-3 HUFA level with varying addition of vitamin E may alter fatty acid composition in the dorsal muscle, but do not affect growth of juvenile rockfish.

• Journal of Animal Science and Technology
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• 제48권2호
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• pp.247-254
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• 2006

본 연구는 보리의 급여수준이 한우수소의 성장과 육질에 미치는 효과를 조사하고자 실시되었다. 수송아지 20두(평균체중 99㎏)를 공시하여 한우수소의 육성비육 시 보리의 급여수준(육성기－비육전기－비육후기 보리 배합비; 0-0-40, FⅡ40; 0-0-60, FⅡ60; 0-20-40, F2040; 0-20-60, F2040)이 증체와 육질에 미치는 효과를 구명하였다. 시험기간 동안의 체중은 성장단계별 보리 급여수준 간 차이는 보이지 않았으나, FⅡ40구의 24개월령 체중이 591.5kg으로 606.2～614.6kg 내외의 다른 처리구에 비해 다소 감소된 경향이었다. 그러나 FⅡ60구는 비육후기에 보리 60% 첨가된 사료를 섭취하면서 증체가 개선되는 경향이 있었다(P<.19). 한우수소에 대한 보리위주 사료의 급여는 생체중, 도체중과 도체율, 정육률, 거래정육률 그리고 KPH 지방과 뼈의 비율 등의 도체특성에 있어 처리 간 차이는 없었으나, 보리를 비육전기에 20%, 비육후기에 60% 급여한 F2060구의 한우수소에서 정육률과 지방률이 각각 89.82, 20.28%로 다른 처리구에 비해 다소 높았으며, 이로 인해 거래정육률은 최대 5.5% 가량 낮게 나타났다. 근내지방도는 FⅡ40구 및 F2060구에서 각각 3.75, 3.60으로 다른 처리구에 비해 높은 편이었으며, 조직감 역시 FⅡ60구와 F2060구가 공히 1.6으로 다른 두 처리구에 비해 양호하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권6호
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• pp.855-860
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• 2005

This study was designed to test the effects of changes in energy and protein intake levels on the maternal milk amino acid concentrations over the short-term in lactating mares. Three lactating mares were enrolled for the study 7 weeks after parturition. A low-energy and low-protein diet (LEP) was administered during the first week of the study, followed by administration of a high-energy and high-protein diet (HEP), again for a week (day 1 to day 7), and milk was sampled thrice daily at intervals of 8 h during the study period. The mean amino acid concentrations in the maternal milk, except for those of proline, serine and valine, were significantly higher in the HEP feeding period than during the LEP feeding period (p<0.05). The sum of the concentrations of all the amino acids (TAA) in the maternal milk samples during the HEP and LEP feeding periods was 1,644.9${\pm}$26.9 and 1,542.3${\pm}$36.0 mg/100 g, respectively, the difference between the two was not significant. When the ratio of each amino acid concentration to the TAA in the maternal milk was analyzed, there were significant differences between the HEP and LEP feeding periods for all amino acids, except glycine, serine, alanine and histidine. It was found that the concentrations of glutamic acid+glutamine, serine, threonine, arginine and valine were significantly higher (p<0.05) on day 1 than on day 7 during the LEP feeding period, and there were no such differences during the HEP feeding period. In regard to the effects of changes in the energy and protein intake levels in lactating mares, no changes in milk amino acid concentrations were found following administration of HEP for a week, whereas 7 days of administration of LEP was associated with a decrease in the amino acid concentrations.

• 운동영양학회지
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• 제13권2호
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• pp.155-160
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• 2009

PURPOSE. The purpose of this study was to investigate the effects of fasting and high-fat diet feeding on uncoupling protein 3 (UCP3) mRNA levels, uncoupling the respiratory chain and producing heat, of skeletal muscle in rat. METHODS. Fasting experiment: Forty Male Sprague-Dawley rats (5 wk) were divided into non-fasting groups (CON) and fasting groups (FG) for 0 day, 0.5 day (12 hr), 1 day, 2 day and 3 day. The rats of CON were sacrificed at 0 and 3 day. High-fat diet experiment: Forty Male Sprague-Dawley rats (5 wk) were divided into low-fat diet groups (LF) and high-fat diet group feeding for 0 day, 0.5 day (12 hr), 1 day, 2 day and 3 day. The rats of LF were sacrificed at 0 and 3 day. Analysis: Analysis of UCP3 mRNA expression was used by Real-time PCR. RESULTS. UCP3 mRNA levels of FG group were increased according to time course for 2 days- fasting but decreased at 3 day-fasting. UCP3 mRNA of HF were increased during HF diet feeding for 2 day, and peaked at 1 day-HF feeding, but decreased 2 day and 3 day-HF feeding CONCLUSION. Therefore, it may be rational that UCP3 is up-regulation when a large amount of fatty acids influx occurs in skeletal muscles as well as might have a role for fine adjustments of energy expenditure.

• Asian-Australasian Journal of Animal Sciences
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• 제19권9호
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• pp.1291-1297
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• 2006

The potential of the spot urine sampling technique as an alternative to performing a total urine collection to predict the microbial nitrogen supply was evaluated in crossbred bulls. In a completely randomized design, 20 growing crossbred bulls were assigned four levels of feed intake (120, 100, 80 and 60% of voluntary dry matter intake) on diets comprised of wheat straw and concentrate mixture (50:50). After three months of experimental feeding, a metabolism trial was conducted for ten days, during which spot urine collections were performed every 6 h post feeding on days 9 and 10. The daily urinary excretion of allantoin (A) and purine derivatives (PD) decreased with the reduction in feed intake while creatinine (C) excretion remained similar in animals fed at different levels. The microbial nitrogen (MN) supply calculated from the PD excreted in total urine (35.08 to 72.08 g/d) was higher at increased levels of feed intake. PD concentration in spot urine samples had poor correlation with feed intake except at 12 h post feeding. A/C ratio and PD/C ratio in spot urine samples remained similar irrespective of sampling time and significantly (p<0.01) correlated with daily urinary PD excretion, digestible organic matter intake and dry matter (DM) intake. However, no significant differences were evident in these ratios among animals fed at levels 120, 100 and 80% of voluntary dry matter intake (VDMI) at different times post feeding. These results suggests that the spot urine sampling technique to predict the microbial protein supply is not suitable for detecting small differences in MN supply and hence, estimation of PD excreted in total urine (mmol/d) is necessary to assess precisely the MN supply in crossbred bulls.

• Asian-Australasian Journal of Animal Sciences
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• 제9권1호
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• pp.107-111
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• 1996

Studies on the effects of protein levels on the total sulphur amino acid (TSAA) requirements of chickens were conducted on the starter broilers by feeding four levels of crude protein(16, 18, 20 and 23%) at three levels of TSAA (0.83, 0.93 and 1.03%) and on the grower broilers by feeding three levels of crude protein (16, 18 and 20%) at three levels of TSAA(0.72, 0.79 and 0.86%). The metabolisable energy of the diets was maintained constant at 3,200 kcal/kg and the experiments were carried out for two growing periods: starter (0-3 wk) and grower (3-6 wk). The results showed that there were significant differences in body weight gain, feed intake and feed:gain ratio under different protein levels of the starters. Crude protein, ME and TSAA intake were significantly affected by increasing the CP levels. The TSAA requirement of the starter broilers is recommended at 0.93% and it is not influenced by different protein levels used in the experiment. For the grower period, body weight gain and feed:gain ratio improved significantly at higher protein diets. Birds fed higher protein diet consumed greater quantities of protein. Responses to TSAA supplementation for body weight gain, feed intake and feed:gain ratio were not significant. The present results showed that the TSAA level of 0.79 to 0.86% was required for grower diets and that the protein levels of the diet did not influence the TSAA requirement.

• Asian-Australasian Journal of Animal Sciences
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• 제8권1호
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• pp.51-58
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• 1995

To examine whether colostral growth factors are transferred to the general circulation, concentrations of plasma cortisol, insulin, prolactin, growth hormone, insulin-like growth factors(IGFs) -I and -II, IGF-binding proteins(IGFBPs) and total protein were measured in newborn calves fed colostrums, milk of milk replacer before and after feeding at 12 h intervals during the first two days after birth. Plasma protein concentrations increased with time after than in milk- or milk replacer-fed calves. The mean protein concentration was greater in colostrum-fed than in milk- or milk replacer-fed calves. Plasma cortisol levels transiently declined after each feeding regardless of the type of diet, while insulin levels tended to increase. Mean concentrations of these hormones did not differ between dietary groups, nor did they change with time after birth. Plasma concentrations of prolactin and growth hormone did not differ between dietary groups and also did not change with time after birth or after feeding. Concentrations of IGF-I and IGF-II transiently increased at the second feeding period, but these, as well as plasma IGFBP profiles, were not different between groups or before and after feeding. Results did not indicate significant transfer of colostral growth factors across the newborn ruminant small intestine.

• Asian-Australasian Journal of Animal Sciences
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• 제7권2호
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• pp.289-294
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• 1994

This report deals with the effect of levels of concentrate supplementary feeding (Nil, 0.25% BW and 0.75% BW) on the productivity of different genotypes (Thai native (TN), 25% Anglo-Nubian (AN), 50% AN and 75% AN) of female goats grazing newly established pasture in a tropical area. The major species of grass was Brachiaria mutica (33%) and of legumes was Centrosema pubescens (34%). There was no significant (p>0.05) difference between nil and 0.25% BW supplementary feeding for growth rate ($g/kg^{0.75}/d$). However, goats fed 0.75% BW supplementary feeding had significantly (p<0.01) higher growth rates than did other treatments. There was no significant (p>0.05) difference among genotypes and between age for growth rate. There was no interaction between feeding and genotype for growth rate.

• 한국가금학회지
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• 제22권4호
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• pp.203-211
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• 1995

In order to investigate the effect of feeding live yeast on growth performance, nutrients utilization, tibia mineral deposit and intestinal microorganism changes, a growth assay was conducted with 360 broiler chicks. Treatments were four levels of yeast as 0, 0.025, 0.05 and 0.1% in 1.83% tricalcium phosphate and two levels of yeast as 0 and 0.05% in 1.15% tricalcium phosphate. The crude protein content of live yeast was 45%, and 97% of it was in the pure protein form, with 46.6% of essential amino acids and 53.4% of non-essential amino acids. Growth performance was tended to increase by feeding the yeast but there was no significant difference(P>.05). The protein digestibility was increased as the feeding level of yeast increased. However, digestibilities of fat, fiber, calcium and phosphorus were not affected by the yeast. Ash and calcium content of tibia were increased as the level of yeast increased. Total number of E. coli in small intestine was significantly decreased(P<.05) in chicks fed yeast. Total number of Lactobaci1lus was significantly increased by the yeast feeding. The changes of microorganism in cecum had the same trend with the changes of microorganism in small intestine.