• 한국작물학회지
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• 제46권4호
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• pp.303-308
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• 2001

To investigate the partitioning of newly absorbed N derived from NO$_3$- and NH$_4$$^{+}$, 6 mM $K^{15}$ NO$_3$ or 3 mM ($^{15}$ NH$_4$)$_2$ was fed continuously in Italian ryegrass (Lolium multiflrum L.) for 7 days. Nitrogen metabolites (nitrate, amino acid, soluble- and insoluble protein) were analyzed at the end of $^{15}$ N feeding. Dry weight in shoot, stubble and root was not significantly different between NO$_3$$^{[-10]}$ and NH$_4$$^{+}$ feeding. Total nitrogen content in all three organs was significantly higher in NH$_4$$^{+}$ than NO$_3$$^{[-10]}$ feeding. Sum on N content in reduced N fractions (amino acids + proteins) in shoot, stubble and roots in NH$_4$$^{+}$ feeding increased by 13.3, 12.5 and 35.4 %, respectively, compared to NO$_3$$^{[-10]}$ feeding. The Relative Specific Activity (RSA, percentage of newly absorbed $^{15}$ N relative to total N in a sample) values of amino acids and insoluble proteins were significantly higher in NH$_4$$^{+}$ feeding. Total amount of newly absorbed $^{15}$ N in NO$_3$$^{[-10]}$ and NO$_3$$^{[-10]}$ feeding was 52.3 and 69.5 mg/plant on dry matter basis, respectively. In both NH$_4$$^{+}$ and NO$_3$$^{[-10]}$ grown plants, most of the N was allocated to the shoot, 67.5% in NH$_4$$^{+}$ feeding and 58.8% NO$_3$$^{[-10]}$ feeding, respectively. The $^{15}$ N amount incorporated in the reduced N compounds (amino acids and proteins) in NH$_4$$^{+}$ grown plants significantly increased by 74.8% compared to NO$_3$$^{[-10]}$ grown plants. The increase of the $^{15}$ N amount assimilated to amino acids in NH$_4$$^{+}$ grown plants was remarkably higher in roots as more than 7.25 times compared to NO$_3$$^{[-10]}$ feeding. These results indicated that Italian ryegrass was much efficiently utilized NH$_4$$^{+}$-N for the synthesis of reduced N compounds.reduced N compounds.

• 한국수산과학회지
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• 제35권2호
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• pp.196-200
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• 2002

우리나라 해산어 양식의 주요 대상 어종인 넙치의 섭식 촉진 물질을 확인하기 위하여 몇 가지 아미노산, 핵산관련물질, 염기성 물질 및 산가수분해물의 섭식촉진 효과를 수조 실험을 통해 검토하였다. Glycine, IMP, GMP 및 betaine은 다소의 섭식 촉진 효과가 인정되었으며 천연 단백질 자원의 산 가수분해물인 KH가 가장 우수한 섭식촉진 효과를 보였다. KH는 사료 중량에 대하여 $1.05\%$ (w/w)까지 섭식촉진 효과가 증가하였으나 그 이상의 농도에서는 효과가 거의 일정하였다. 산 가수분해물의 pH는 섭식촉진에 영향을 미치지 않았고, 물질의 흔합에 따른 상승효과는 KH와 glycine의 조합이 가장 우수하였다. 대조군 사료는 총 급이량의 $58.2\%$를 섭취한 반면 섭식촉진 사료는 $83.3\%$를 섭취하여 섭식촉진물질의 첨가는 섭식량의 증가 뿐 아니라 사료 효율의 개선에 현저히 기여하였다. 그리고 섭식촉진 물질의 조합에 따른 단가 상승은 기본사료 가격의 $0.05\%$ 이내에 불과하여 경제성이 충분하며 실용화가 가능한 것으로 예상하였다. 한편 본 연구 결과의 적용을 확대하기 위해서는 장차 넙치 성어에 대한 연구도 필요할 것으로 보인다.

• Journal of Animal Science and Technology
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• 제66권1호
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• pp.135-144
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• 2024

The objective of this study was to investigate the influence of feeding frequency on a sow's reproductive performance and stress response during gestation. A total of twenty multiparous sows (Yorkshire × Landrace) were used in a completely randomized design based on their parity, body weight (BW), and backfat thickness (BFT), and the sows were allotted to two different feeding systems: 1) once daily feeding (OF) and 2) twice daily feeding (TF) in corn-soybean meal based diets. The gestation diet was formulated to contain 3,265 kcal of metabolizable energy (ME) / kg, 12.90% of crude protein (CP), and 0.75 % of total lysine. The lactation diet was formulated to contain 3,265 kcal of ME / kg, 16.80% of CP, and 1.08% of total lysine and provided ad libitum during lactation. In gestation, sow BFT and BF changes were not affected by feeding frequency, but higher BW and BW gain from day 35 to 90 and day 35 to 110 were observed in OF sow (p < 0.10). In lactation, feeding frequency did not influence on BW, BW gain, BFT, BF changes, average daily feed intake, and wean-to-estrus interval. Also, there were no differences in litter size, litter weight and piglet weight in lactating sows. OF sows had higher (p < 0.05; p < 0.10) protein, solid-not-fat, and total solid concentrations in colostrum compared to TF sows, while OF sows had a lower (p < 0.05) lactose concentration in colostrum compared to TF sows. Sows in OF showed significantly lower average daily water consumption (ADWC) from day 35 to 110 of gestation (p < 0.05). While there were no significant differences in stereotypic behaviors and salivary cortisol levels during gestation between treatments, the OF sows showed less time spending on the activity at day 105 (p < 0.05). In conclusion, reduced feeding frequency increased BW gain during gestation, decreased activation time, and changed the colostrum composition. This information may contribute to the understanding of the physiological and behavioral change of gestating sows by manipulating feeding frequency.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.194-197
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• 2000

The color of textile-wastewater hindered spectrometric measurements of $H_2O_2$ and enzyme activity during enzymatic decoloring. By using ABTS, we developed a new method for measuring peroxidase activity and $H_2O_2$ concentration. The ratio of enzyme and $H_2O_2$ was optimized as 1:150 by investigating the effects of $H_2O_2$ on enzymatic decoloring. Pulse feeding of $H_2O_2$, upon depletion, significantly increased the decoloring of Congo Red.

• Animal cells and systems
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• 제8권4호
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• pp.329-333
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• 2004

Japanese flounder (Paralichthys olivaceus) head kidney (HK) leukocytes were incubated with $10^3$ to $10^{-3}$ ng levamisole/ml for 4, 24 or 48 h and then assayed for their natural cytotoxic activity against xenogeneic tumor cells. This activity was slightly increased after 24 h of incubation. In a second experiment, fish were fed 0, 75, 150 or 300 mg levamisole/kg diet for 10 consecutive days. The fish were then fed a commercial non-supplemented diet and sampled 0, 1, 2, 3, 4 or 6 weeks post-administration of levamisole. The cytotoxic activity was found to be increased with increasing levamisole dose and remained greatly enhanced until the end of the experiment. In conclusion, levamisole enhanced flounder natural cytotoxic cell activity both in vitro and in vivo and had a great lasting action when administered by feeding.

• 한국수산과학회지
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• 제40권5호
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• pp.300-307
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• 2007

This study investigated the effects of dietary supplementation with hot water extracts (HE) of selfheal (Prunella vulgaris) and Lactobacillus rhamnosus culture fluid (LF) on the growth and non-specific immune responses of juvenile olive flounder. A total of 270 fish ($5.07{\pm}0.01g$, average $weight{\pm}SD$) were divided randomly into nine groups, and three groups were fed one of three isonitrogenous (51% crude protein) and isocaloric (17.6 MJ/kg) diets. The diets contained no supplement, 50 mL hot water extract, or 50 mL L. rhamnosus fluid (designated as diets CON, HE and LF, respectively). During the 8-week feeding trial, the growth, feed utilization and survival of the fish were not significantly affected by the experimental diets. There were no significant differences in the hematocrit and hemoglobin of fish fed each of the experimental diets. However, the serum alanine aminotransferase and aspartate aminotransferase activity were lower with the dietary supplements containing HE and LF. The alanine aminotransferase activity of fish fed the HE diet was significantly lower than that of fish fed the control diet. The hematocrit, hemoglobin, and nitroblue tetrazolium (NBT) activity were higher 3 hr after feeding than 24 hr afterwards. The NBT activity of fish fed the HE and LF diets were significantly higher than that of fish fed the control diet 3 hr after feeding. The findings suggest that dietary supplementation with HE and LF could enhance the nonspecific immune responses of juvenile olive flounder.

• 한국응용곤충학회:학술대회논문집
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• 한국응용곤충학회 2005년도 춘계 학술발표회
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• pp.173-173
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• 2005

• 한국응용곤충학회지
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• 제32권3호
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• pp.354-371
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• 1993

컴퓨터와 자동감지장치를 이용하여 먹바퀴 성충 암컷의 행동을 사육상에서 10~15일간 연속 관찰하였다. 대체적으로 12L~12D의 광조건하에서 각 미소서식처에서의 출현율과 활동성에는 개체의 변이가 컸고 일주기성이 낮게 나타났다. 먹이, 물 타개체가 공급되었을 경우 휴식처, 타개체, 먹이 및 물이 있는 것에서 각각 하루 평균 20.42, 11.50, 6.31, 2.66% 출현했다. 먹이, 물 타개체를 공급하지 않았을 경우 휴식처에서 하루중 20.29% 출현하였다. 출현 시간당 방문 횟수는 다른 미소서식처에서보다 먹이 먹는 곳과 물 마시는 곳에서 많았고 먹바퀴의 활동성은 먹이, 물, 타개체를 공급했을 때가 공급하지 않았을 때 보다 높았다. 미소서식처 출현에 대한 Principal Component Analysis (PCA) 결과 각 개체들의 출현 양상은 다양하게 나타났으나 (Q모드), 각 시간대는 명, 암기로 유형화 되어 나타났다(R모드). 먹이, 물, 타개체 공급시 휴식처에서의 출현이 먹이를 먹는 곳과 물을 마시는 곳에서의 출현보다 명, 암기의 유형성이 강하게 나타났다. 각 미소서식처에서의 출현율과 활동을 종합적으로 고려하여 PCA한 결과 먹바퀴의 활동은 먹이 및 타개체가 있는 것의 출현율과 관련 되었다.

• Animal Bioscience
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• 제36권1호
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• pp.75-83
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• 2023

Objective: The objective of this study was to describe a methodological procedure to quantify the heat production (HP) partitioning in basal metabolism or fasting heat production (FHP), heat production due to physical activity (HPA), and the thermic effect of feeding (TEF) in roosters. Methods: Eighteen 54-wk-old Hy Line Brown roosters (2.916±0.15 kg) were allocated in an open-circuit chamber of respirometry for O₂ consumption (VO₂), CO₂ production (VCO₂), and physical activity (PA) measurements, under environmental comfort conditions, following the protocol: adaptation (3 d), ad libitum feeding (1 d), and fasting conditions (1 d). The Brouwer equation was used to calculate the HP from VO₂ and VCO₂. The plateau-FHP (parameter L) was estimated through the broken line model: HP = U×(R-t)×I+L; I = 1 if t<R or I = 0 if t>R; Where the broken-point (R) was assigned as the time (t) that defined the difference between a short and long fasting period, I is conditional, and U is the decreasing rate after the feed was withdrawn. The HP components description was characterized by three events: ad libitum feeding and short and long fasting periods. Linear regression was adjusted between physical activity (PA) and HP to determine the HPA and to estimate the standardized FHP (st-FHP) as the intercept of PA = 0. Results: The time when plateau-FHP was reached at 11.7 h after withdrawal feed, with a mean value of 386 kJ/kg^0.75/d, differing in 32 kJ from st-FHP (354 kJ/kg^0.75/d). The slope of HP per unit of PA was 4.52 kJ/mV. The total HP in roosters partitioned into the st-FHP, termal effect of feeding (TEF), and HPA was 56.6%, 25.7%, and 17.7%, respectively. Conclusion: The FHP represents the largest fraction of energy expenditure in roosters, followed by the TEF. Furthermore, the PA increased the variation of HP measurements.

• 재활치료과학
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• 제11권3호
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• pp.51-64
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• 2022

목적 : 본 연구의 목적은 뇌성마비 섭식장애 평가의 영역을 수립함과 동시에 뇌성마비 섭식장애 평가 시 범용적으로 사용할 수 있는 평가 항목을 개발하고자 한다. 연구방법 : ICF 분류기준에 근거하여 섭식장애 중재에 포함되어야 하는 평가 및 치료 영역 등을 문헌 고찰하여 평가영역을 종합하고, 전문가의 검증을 거쳐 델파이 조사지 초안을 제작하였다. 그 후 20명의 전문가를 대상으로 1차 및 2차 델파이 연구가 진행되었다. 결과 : 뇌성마비의 섭식 영역을 6개 영역으로 범주화하고, 항목을 구성하였다. 전문가 자문을 통하여 평가 영역 및 항목들을 체계화하였으며, 전문가 자문을 거친 평가 영역과 항목들은 1차, 2차 델파이 조사를 통하여 6개 영역, 9개의 중분류, 32개의 소분류, 76개의 항목으로 최종 확인되었다. 결론 : 본 연구에서는 특정 영역을 평가하는 항목 구성으로 된 기존의 평가도구에서 벗어나 평가 영역 확장 및 평가 항목을 체계적으로 구성하였다는데 의미가 있다고 할 수 있다. 따라서 본 연구결과를 바탕으로 섭식장애를 가진 뇌성마비 아동의 치료적 중재 활동 시에 아동의 현재 섭식 기능 및 활동 수준을 확인하고자 하는 작업치료사에게 유용하게 사용될 것으로 판단된다.