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Effects of Achyranthes Bidentata Polysaccharide on Growth Performance, Immunological, Adrenal, and Somatotropic Responses of Weaned Pigs Challenged with Escherichia coli Lipopolysaccharide

  • Guo, Guanglun;Liu, Yulan;Fan, Wei;Han, Jie;Hou, Yongqing;Yin, Yulong;Zhu, Huiling;Ding, Binying;Shi, Junxia;Lu, Jing;Wang, Huirong;Chao, Jin;Qu, Yonghua
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.8
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    • pp.1189-1195
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    • 2008
  • A study was conducted with 48 weaned barrows ($28{\pm}3d$, $8.45{\pm}0.14kg$) to determine the effect of Achyranthes bidentata polysaccharide (ABPS) supplementation on pig performance, immunological, adrenal and somatotropic responses following Escherichia coli lipopolysaccharide (LPS) challenge. The experiment was a $2{\times}2$ factorial design; the main factors included diet (supplementation with 0 or 500 mg/kg ABPS) and immunological challenge (LPS or saline). On d 14 and 21 of the trial, pigs were given an intraperitoneal injection with either $100{\mu}g/kg$ BW of LPS or an equivalent amount of sterile saline. Blood samples were obtained 3 h after injection for analysis of tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$), prostaglandin $E_2$ ($PGE_2$), cortisol, growth hormone (GH), insulin-like growth factor (IGF)-I and immunoglobulin G (IgG). On d 2 after LPS challenge, peripheral blood lymphocyte proliferation (PBLP) was measured. LPS administration decreased average daily feed intake (ADFI) (p<0.05), had a tendency to decrease average daily gain (ADG) (p<0.10) during both the first and second challenge periods and increased (p<0.05) feed:gain ratio only during the first challenge period. ABPS tended to improve ADG (p<0.10) during the first challenge period, and improved ADG (p<0.05) and tended to improve ADFI (p<0.10) during the second challenge period. ABPS did not affect feed:gain ratio. An interaction (p<0.05) between LPS challenge and diet was observed for the plasma concentrations of TNF-${\alpha}$, $PGE_2$ and cortisol after both LPS challenges such that, among LPS-treated pigs, pigs fed the ABPS diet were lower for these indices than those receiving the control diet. In contrast, pigs fed the ABPS diet had higher IGF-I (p<0.05) compared with those fed the control diet. No effect of diet, LPS challenge or both on GH and IgG was observed after both LPS administrations. LPS challenge increased PBLP when these cells were incubated with $8{\mu}g/ml$ of LPS during both the challenge periods, and did likewise when incubated with $8{\mu}g/ml$ of concanavalin A only after the first challenge. ABPS had no effect on PBLP. These data demonstrate that ABPS alters the release of pro-inflammatory cytokines following an immunological challenge, which might enable pigs to achieve better performance.

Effects of Feed Additive as an Alternative for Antibiotics on Growth Performance and Feed Cost in Growing-finishing Pigs (항생제 대체제로서 첨가제가 육성 및 비육돈의 성장능력과 사료비에 미치는 영향)

  • Kim, Jong-Duk;Abuel, Sherwin J.;Shim, Keum-Seob
    • Korean Journal of Organic Agriculture
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    • v.18 no.2
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    • pp.233-244
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    • 2010
  • This experiment was conducted to evaluate two different types of feed additive as an alternative for antibiotics on growth performance and feed cost in growing and finishing pigs. One additive is an herb extract, with Fenugreek (Trigonella foenum graecum) as the main component, while the other feed additive contains aminolevulinic acid (ALA). In the first experiment, 128 grower pigs were allotted to 4 different treatment groups and replicated 4 times with 8 pigs per replicate; the trial lasted for 28 days. The positive control group (PC) which is the control group supplemented with antibiotics was significantly higher (p<0.05) in growth rate (580.6 g/d) followed by the ALA group (532.0 g/d), there was no significant differences in terms of feed intake and feed efficiency. There were marginal reductions in feed costs measured as feed cost per head in ALA and HE added diet. However, the feed cost per weight gain of ALA treatment was higher than the control group (PC) supplemented with antibiotics. In the second experiment, 80 finisher pigs were allotted to 4 treatment groups and replicated 4 times with 5 pigs per replicate; the trial lasted for 70 days. The treatment group supplemented with an herb extract (HE) had a significantly higher (p<0.05) feed intake (2,415.8 g/d) compared to the other treatment groups, but there was no significant differences in terms of growth rate and feed efficiency. Feed cost per head in HE and ALA treatments were higher than PC treatment, and feed cost per weight gain of HE was higher than PC treat (p<0.05). The results from these experiments suggests that these two types of feed additives can both be used as an alternative for antibiotics without having a negative effect on the performance of the animals. And aminolevulinic acid was good in performance and production cost of grower and finisher pigs.

Economic Evaluation of The Newly Developed Fish Meal Analog (BAIFA-M) (양어사료의 어분대체품 개발의 경제성 분석)

  • 김기수;배승철;최재영;김우경
    • The Journal of Fisheries Business Administration
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    • v.29 no.1
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    • pp.121-137
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    • 1998
  • This study was conducted to examine the economic evaluation of the newly developed fish meal analog (BAIFA-M) in Korean rockfish feed. A raw fish feed (MP) and two commercially formulated diets (EP) were employed to compare weight gain (WG), feed conversion ratio (FCR) and survival rate (SR) from the sea cage culture system. By using the economic model in the practical sea cage culture system, feed cost, production cost and gross profit per kg production, rate of profit to gross revenue (RPGR), and internal rate of return (IRR) were calculated based upon the results of the experiment and the information of the private aquaculture farm (Young Chang) in Tong young, Korean. IRR criteria is one of the popular economic feasibility analysis methods applicable far aquaculture industry. This is an economic evaluation method to compare the given interest rate or the discount rate with IRR which can be calculated by the difference between the present value of the benefit stream and of the cost stream. The benefits of using EP on WG, FCR, SR, and production cost will be emphasized in this study. Fish averaging 20$\pm$3.6g (Mean$\pm$SD) were randomly distributed in each small cage (6m$\times$6m) as groups of 2,000 fish. By using 3 large size cages (12m$\times$12m), 12 small cages were constructed, and only 9 small cages were employed for three replicates of each diet treatment. To compare with MP diet, two sinking EP diets were designed by our laboratory and produced by the local feed company who wanted to promote these EP diets for the mass cage culture of Korean rockfish in the future. Two EP diets contain white fish meal and/or BAIFA-M as the main animal protein sources : WFM diet, maximum 43% of white fish meal : BAIEA- M diet, 30% of white fish meal nab replaced by BAIFA- M from WFM diet. Results are summarized in Table 1. Fish fed MP diet showed significantly lower SR than does fish fed two EP diets(P<0.05). However, there Were no significant difference on FCR among fish fed three practical diets. Table 1. Average feed conversion ratio (FCR), accumulative average survival rate (SR) and economic evaluation data far three practical diets. As we expected, BAIFA-M diet is more economical than WFM diet as well as MP diet. Feed cost and production cost per kg production from BAIEA - M diet were lower than those from WFM and MP diets. Moreover, gross profit per Kg production, RPGR and IRR from BAIFA- M diet were higher than those from WFM and MP diets. This economic evaluation study clearly indicated that MP diet should be replaced by the commerical formulated EP diets as soon as possible in the near future because MP diet is not economical in the practical sea cage culture system.

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The effect of intermittent feeding and cold water on performance and carcass traits of broilers reared under daily heat stress

  • Erensoy, Kadir;Noubandiguim, Moise;Sarica, Musa;Aslan, Resul
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.12
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    • pp.2031-2038
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    • 2020
  • Objective: This study aimed to determine the effect of intermittent feeding and cold water on performance and carcass traits in broiler chickens between 4 to 6 wk of age exposed to daily high temperature. Methods: Broilers were assigned to four treatment groups according to a 2×2 factorial design between 22 to 42 d of age (80 broilers per treatment, 4 replications). Broilers were divided into two main groups as feeding type (ad-libitum [AL] and intermittent [IF] for 6 h daily) and sub-groups as water temperature (normal [NW], 24.9℃ and cold [CW], 16.4℃). Heat treatment was applied between 11.00 to 17.00 h daily between 22 to 42 d of age. Results: Live weight at 6th wk was not affected by feeding type and water temperature, but the live weight was significantly higher in IF chickens at the 5th wk (p<0.05). Average weekly gain of IF broiler chickens were higher compared to AL group at 4, 5, and 6 wk of age (p< 0.05). Although feeding type did not affect feed intake in 4 and 5th wk, feed intake was higher in IF chickens at 6th wk (p<0.01). In addition, feeding type and water temperature did not affect feed conversion ratio and interactions were not significant (p>0.05). Water temperature had no significant effect on heart, liver, gizzard, and abdominal fat percentages (p>0.05). Conclusion: It is concluded that IF increased the average weekly gain in chickens reared under daily heat stress for 6 h between 22 to 42 d of age. IF in hot environmental conditions slightly increased performance without adversely affecting health, welfare, and physiological traits, whereas CW implementation had no significant effect on performance. It can also be said that IF suppresses a sudden increase in body temperature depending on age and live weight.

Fish Diseases and Their Control in High Density Culture of Eel (고밀도 뱀장어 양식수조의 질병대책)

  • CHUN Seh Kyu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.16 no.2
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    • pp.103-110
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    • 1983
  • The main purpose of the present study is to evaluate a revolving plate type biofilter system for mass culture of eel(Anguilla japonica) based on the experimental rearing for 120 days (Oct. 1982-Feb. 1983). Water quality changes, growth efficiency of fish and fish disease treatment were critically evaluated. A revolving plate type biofilter system was designed(Fig. 1). The system consisted of a glass tank (150 l), a revolving plate biofilter and a settling tank(150 l). The biofilter consisted of 60 submerged quadriangular plates ($28{\times}37$ cm) and 30 revolving plates (32 cm diameter) for a total of 19.0 $m^2$ of surface area. The revolving plates were made to rotate 10 time per minute, The total water volume of the rearing system were 300 l, and everyday 1/3 of the total water volume were changed with freshly prepared water. In the rearing system a total of 2 kg of eel (1,500 individuals, mean weight:1.3 g) were reared fed on the pellet feed and the dough feed. The growth efficiency were much better for the pellet feed (FC: 1.79) compared to the dough feed (FC: 3.56). During the experimental rearing water quality control was satisfactory. Total ammonia concentrations were 0.38-0.59 ppm and nitrite concentration were 0.83-1.19 ppm. On the other hand alkalinity decreased from 176ppm just after the water change to 17ppm just before the water change. The low alkaline condition was compensated by the regular change of water. Epidemics of parasitic gill-flocks (Pseudodactvlogylus sp.) was observed, and they were easily eliminated by the treatment of DDVP (1.0 ppm). Trichodina sp. and Costia sp. were observed, and they were also controlled by the treatment of potassium permanganate (4.0 ppm).

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갈조류의 영양적 특성과 가금에서 기능성 물질로서의 갈조류 추출물의 이용

  • 심재민;안병기;강창원
    • Proceedings of the Korea Society of Poultry Science Conference
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    • 2003.11a
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    • pp.38-52
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    • 2003
  • Seaweeds are large algae that grow in a saltwater or marine environment. There are over 400 species of seaweeds in the bay of Korea. Most seaweeds are divided into three groups according to their color : the green seaweeds(division Chlorophyta), the brown(Phanphyta) and the red(Rhodophyta), Seaweeds have been harvested for use as food, fertilizer, animal feed and medicines for thousands years. In the past decade, there has been increasing interest in using seaweeds for animal feedstuffs and feed supplements as their health qualities are becoming better known. In this review some perspectives on nutritional values and the role of functionality ingredients of various seaweeds will be also discussed. Ecklonia cava kjellman(EC), a perennial seaweed, is one of the main components of the marine forests along the East and South coasts of Korea. We conducted three experiments to evaluate the nutritional values of EC and the crude lectin extracted residues from EC(LEEC) and to investigate their dietary effects on productivity and immunocompetence in poultry. The crude protein content of EC was relatively low(about 10 %) and its amino acids were poorly available. But, the results of our study suggested that EC can be used into broiler feed up to 3 % without any adverse effect, if its nutritional values are well evaluated prior to use. Similar results of dietary LECC up to 1.0 % level on egg production and egg quality have been obtained in layers. The concentrations of serum TNF-${\alpha}$ after Salmonella challenge were rapidly increased in accordance with the level of LEEC. These results indicated that LEEC can be used as a feed additives for enhancing of immunocompetence in poultry.

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Characterization of a Thermostable Lichenase from Bacillus subtilis B110 and Its Effects on β-Glucan Hydrolysis

  • Huang, Zhen;Ni, Guorong;Wang, Fei;Zhao, Xiaoyan;Chen, Yunda;Zhang, Lixia;Qu, Mingren
    • Journal of Microbiology and Biotechnology
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    • v.32 no.4
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    • pp.484-492
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    • 2022
  • Lichenase is an enzyme mainly implicated in the degradation of polysaccharides in the cell walls of grains. Emerging evidence shows that a highly efficient expression of a thermostable recombinant lichenase holds considerable promise for application in the beer-brewing and animal feed industries. Herein, we cloned a lichenase gene (CelA203) from Bacillus subtilis B110 and expressed it in E. coli. This gene contains an ORF of 729 bp, encoding a protein with 242 amino acids and a calculated molecular mass of 27.3 kDa. According to the zymogram results, purified CelA203 existed in two forms, a monomer, and a tetramer, but only the tetramer had potent enzymatic activity. CelA203 remained stable over a broad pH and temperature range and retained 40% activity at 70℃ for 1 h. The Km and Vmax of CelA203 towards barley β-glucan and lichenan were 3.98 mg/ml, 1017.17 U/mg, and 2.78 mg/ml, 198.24 U/mg, respectively. Furthermore, trisaccharide and tetrasaccharide were the main products obtained from CelA203-mediated hydrolysis of deactivated oat bran. These findings demonstrate a promising role for CelA203 in the production of oligosaccharides in animal feed and brewing industries.

Surface roughness prediction with a full factorial design in turning (완전요인계획에 의한 선삭가공시 표면거칠기 예측)

  • Yang, Seung-Han;Lee, Young-Moon;Bae, Byong-Jung
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.1 no.1
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    • pp.133-140
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    • 2002
  • The object of this paper is to predict the surface roughness using the experiment equation of surface roughness, which is developed with a full factorial design in turning. $3^3$ full factorial design has been used to study main and interaction effects of main cutting parameters such as cutting speed, feed rate, and depth of cut, on surface roughness. For prediction of surface roughness, the arithmetic average (Ra) is used, and stepwise regression has been used to check the significance of all effects of cutting parameters. Using the result of these, the experimental equation of surface roughness, which consists of significant effects of cutting parameters, has been developed. The coefficient of determination of this equation is 0.9908. And the prediction ability of this equation was verified by additional experiments. The result of that, the coefficient of determination is 0.9718.

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A Study on ZVT Boost Converter Using a ZCS Auxiliary Circuit (ZCS 보조회로를 이용한 ZVT Boost 컨버터에 관한 연구)

  • Ryu D.K.;Lee W.S.;Choi T.Y.;Seo M.S.;Won C,Y.;Kim Y.R.
    • Proceedings of the KIPE Conference
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    • 2001.12a
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    • pp.129-132
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    • 2001
  • Recently, a ZVT boost converter is embedded in a power factor correction system. The control circuit of the converter assures soft-switching for all the MOSFETs and load regulation. The PFC system contains additional control circuits which assure the input voltage in a sinusoidal form and feed-forward line voltage regulation. In this paper, a soft switching boost converter with zero-voltage transition(ZVT) main switch using zero-current switching(ZCS) auxiliary switch is proposed. Operating intervals of the converter are persented and analyzed. The proposed results show that the main switch maintains UT while auxiliary switch retains ZCS for the complete specified line and load conditions.

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Chip Formation of Ceramic Tools in Metal Cutting (절삭가공에서 세라믹 공구의 칩형상에 관한 연구)

  • 노상래;안상욱
    • Journal of the Korean Ceramic Society
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    • v.31 no.11
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    • pp.1355-1361
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    • 1994
  • With the availability of ceramics (Al2O3, Al2O3-TiC), it is possible to machine very hard steel at different cutting conditions. When hardened steel STD 11 is turned using ceramic tools, chip formation is observed conical-herical and arc chips with a cyclic saw toothed type. The main cause of saw toothed chip formation is observed conical-herical and arc chips with a cyclic saw toothed type. The main cause of saw toothed chip formation is found to be periodic gross shear fracture extending from the free surface of the chip toward the tool tip. In regard to chip control, ceramic Al2O3 is superior to the other cutting tools. The roughness of machined surface was getting worse with increasing of cutting speed and feed.

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