• 한국산학기술학회논문지
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• 제19권12호
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• pp.836-846
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• 2018

본 연구는 졸업생의 교육목표 달성도, 비판적 사고 성향과 임상수행능력 정도를 파악하고, 교육목표 달성도와 비판적 사고 성향, 임상수행능력 간의 상관관계와 교육목표 달성도에 영향하는 요인을 확인하고자 시행하였다. 연구대상자는 간호학과를 졸업 한 후 병원에서 근무하는 간호사로서 대학 졸업 후 5년 이내 졸업생 82명과 그 졸업생을 지도하는 간호관리자 68명이였다. 자료는 2014년 1월부터 3월까지 수집하였다. 수집한 자료는 SPSS/WIN 23.0 프로그램을 이용하여 기술통계, t-test, ANOVA, pearson correlation과 multiple regression으로 분석하였다. 연구 결과 졸업생의 교육목표 달성도는 평균 $3.60{\pm}0.60$점/6점, 비판적 사고 성향은 평균 $3.46{\pm}0.28$점/5점, 임상수행능력은 평균 $4.17{\pm}0.56$점/6점이었다. 교육목표 달성도는 비판적 사고 성향(r=0.52, p<.001), 임상수행능력(r=0.52, p<.001)과 양의 상관관계를 보였다. 교육목표 달성도에 영향을 미치는 요인은 비판적 사고 성향(${\beta}=.30$, p=.018)과 임상수행능력(${\beta}=.26$, p=.029)이었고, 회귀분석 모형의 설명력은 34.25%이었다(F=6.65, p<.001). 간호단위 관리자가 평가한 졸업생들의 교육목표 달성도와 임상수행능력이 졸업생들이 자가 평가한 것보다 높았다(p<.001). 이러한 결과는 앞으로 교육목표 재설정과 교육과정 개선에 반영될 것이며, 교육목표 달성도를 높이기 위한 전략을 수립하는 데 기초자료로 활용할 수 있을 것이다.

• 한국산학기술학회논문지
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• 제20권7호
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• pp.145-150
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• 2019

본 연구는 동물복지 인증기준의 개선을 위해 농가 현장조사를 실시하였으며, 더불어 국내 농가환경에 적합한 동물복지 수준평가 방법을 개발하고자 일부 인증 농가를 대상으로 동물복지 수준의 기초자료를 확보하고자 조사를 실시하였다. 연구조사의 대상은 동물복지 산란계 인증농가 10개소를 선정하였으며, 산란계 인증농가 사육시설 조사는 사육형태, 사육두수, 사료와 음수 공간, 사육밀도, 횃대의 길이 및 모양을 수집하였다. 동물복지 수준 평가는 모래목욕의 유무, 산란상 사용, 환경풍부화물 제공, 방사장 제공, 깃털손상, 쪼기에 의한 상처 유무를 조사였다. 연구결과는 동물복지 산란계 인증농가들의 대부분이 인증기준을 준수하고 있었다. 일부 농가에서 과도한 인증기준을 지적하였는데 실제 농가내부에 급이기, 급수기 및 횃대의 시설로 인해 닭들이 움직일 수 있는 공간이 제한적인 것으로 판단되었다. 모든 농가들에서 산란상을 제공하고 있었으나 방란율이 20%이상인 농가도 있어 산란상 활용에 문제가 있음을 확인하였다. 횃대의 경우 길이는 충족하고 있었으나 10개 농가 중 3개 농가에서만 둥근형태의 횃대를 사용하고 있었다. 모든 농가에서 깔짚을 제공하고 있었으나 보충 또는 교체가 이루어지지 않아 바닥이 단단해져 닭들이 모래목욕행동을 보이지 않는 농가도 2개소나 되었다. 환경풍부화물의 경우 사료 외에 풀사료 등을 제공하는 농가는 4개소로 조사되었다. 대부분의 조사농가에서 깃털손상이 확인되었는데 이것은 조사농가 모두 유정란을 생산하고 있어 수탉에 의한 깃털손상일 것으로 판단된다. 본 연구에서 10개소의 농가만 조사하였기 때문에 전체 인증농가를 대표할 수는 없으나 일부항목에서 조사농가들 사이에서도 큰 차이를 보이고 있어 동물복지 수준평가에 면밀한 분석이 이루어져야 할 것으로 판단된다.

• Journal of Animal Science and Technology
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• 제64권1호
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• pp.166-182
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• 2022

Deer antler velvet is widely used in traditional medicine for its anti-aging, antioxidant, and immunity-enhancing effects. However, few studies have reported on the discovery of probiotic strains for deer antler fermentation to increase functional ingredient absorption. This study evaluated the ability of probiotic lactic acid bacteria to enhance the concentrations of bioactive molecules (e.g., sialic acid and gamma-aminobutyric acid [GABA]) in extracts of deer antler velvet. Seventeen strains of Lactobacillus spp. that were isolated from kimchi and infant feces, including L. sakei, L. rhamnosus, L. brevis, and L. plantarum, and those that improved the life span of Caenorhabditis elegans were selected for evaluation. Of the 17 strains, 2 (L. rhamnosus LFR20-004 and L. sakei LFR20-007) were selected based on data showing that these strains increased both the sialic acid and GABA contents of deer antler extract after fermentation for 2 d and significantly improved the life span of C. elegans. Co-fermentation with both strains further increased the concentrations of sialic acid, GABA, and metabolites such as short-chain fatty acids and amino acids. We evaluated the biological effects of the fermented antler velvet (FAV) on the antibacterial immune response in C. elegans by assessing worm survival after pathogen infection. The survival of the C. elegans conditioned with FAV for 24h was significantly higher compared with that of the control worm group fed only normal feed (non-pathogenic E. coli OP50) exposed to E. coli O157:H7, Salmonella typhi, and Listeria monocytogenes. To evaluate the protective effects of FAV on immune response, cyclophosphamide (Cy), an immune-suppressing agent was treated to in vitro and in vivo. We found that FAV significantly restored viability of mice splenocytes and immune promoting-related cytokines (interleukin [IL]-6, IL-10, inducible nitric oxide synthase [iNOS], interferon [IFN]-γ, and tumor necrosis factor [TNF]-α) were activated compared to non-fermented deer antlers. This finding indicated the protective effect of FAV against Cy-induced cell death and immunosuppressed mice. Taken together, our study suggests that immune-promoting antler velvet can be produced through fermentation using L. rhamnosus LFR20-004 and L. sakei LFR20-007.

• 대한본초학회지
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• 제37권6호
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• pp.45-52
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• 2022

Objective : In this study, we conducted to analysis the patents about Korean medicine based treatment technology for pet. Methods : We collected the Korean, Japan, USA, China, EU and PCT patent for the Korean medicine-based treatment technology for pet using WIPSON data base registered by October 4, 2020 were selected. Results : Patents for the development of pet Korean medicine-based treatment technology have been constantly applied since the mid-2000s, starting in 1990, with a total of 84 applications. The number of patent applications filed in Korea was the largest, after Japan and China. The detailed composition of the applications reported until now is pharmaceutical compositions for the treatment of pet were the most applied with 39%(33 cases), followed by feed used as stocks for pet at 36%(30 cases). According to the patent application rate by disease, most of applications rate composed to be associated with immunity in 2009 to 2013 with 40% of applications. In 2014 to 2018 43% of patent applications are associated with gastrointestinal/digestive system. In this regard, multiple applications were filed in the order of immunity, digestive system, obesity and urinary system. Conclusion : This study analyzed the trend of 84 patents about Korean medicine-based treatment technology for pet to date to establish the basis for future research and development. There were many applications related to immunity from 2009 to 2013, and since then, related to digestive disease from 2014 to 2018.

• Journal of Animal Science and Technology
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• 제64권3호
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• pp.574-587
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• 2022

This study was performed to development the alternative farrowing pen (AFP) and to investigate performance and behavior of lactating sows and their litter. A total of 64 multiparous sows were randomly divided into two groups and were allocated to farrowing crates (FCs) and AFPs. The AFPs contained a crate and support bars that could be folded to provide the sows with extra space on day 5 postpartum. Behavior was recorded by charge-coupled device cameras and digital video recorders, and the data were scanned every 2 min to obtain an instantaneous behavioral sample. Farrowing systems did not affect feed intake, back-fat thickness, litter size and piglet weight at birth and weaning (p > 0.05). In addition, there were no differences in the number of crushed piglets between the two farrowing systems (p > 0.05). However, the weaning-to-estrus interval was shorter in the sows of the AFPs than in thous of the FCs (p < 0.05). The sows spent most of their time lying down during the lactating period, at about 80% lateral recumbency and 10%-15% ventral recumbency. The only significant differences were in the feeding and drinking behavior between sows in the two farrowing systems (p < 0.05). The FC sows displayed more feeding and drinking behavior than the AFP sows, especially in the late lactating period (p < 0.05). Piglets in the FCs tended to spend more time walking than piglets in the AFPs (p < 0.05), whereas there were no differences in suckling and lying behavior between piglets in the two farrowing systems (p > 0.05). It is concluded that the AFPs with temporary crating until day 4 postpartum did not negatively affect performance and crushed piglet compared with the FCs. It also may improve animal welfare by allowing sows to move and turn around during the lactating period. Further research is needed to find suitable housing designs to enhance productivity and animal welfare.

• Animal Bioscience
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• 제36권8호
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• pp.1293-1303
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• 2023

Objective: Inflammation and pain management in postpartum hyperprolific sows is currently an important animal welfare issue in the swine industry. The present study investigates effects of ketoprofen treatment on the incidence of post-parturient disorders, feed intake, colostrum yield, piglet colostrum intake, colostrum immunoglobulin G (IgG) and piglet mortality rate during the first 3 days of postnatal life. Methods: In total, 61 Danish Landrace×Yorkshire crossbred sows and their offspring (n = 833) were included in the experiment. The sows were randomly distributed into two groups: i) control (n = 31), sows were treated with tolfenamic acid 2 mg per kg for 2 days postpartum; ii) ketoprofen (n = 30), sows were treated with ketoprofen 3 mg per kg for 2 days postpartum. The farrowing process of the sows was monitored for 24 h daily, and data associated with farrowing were collected. Piglet colostrum intake, sow colostrum yield and colostrum IgG were determined. Results: During the first 3 days postpartum, the incidence of sows that had fever did not differ between control and ketoprofen groups (51.6% and 56.7%, respectively, p = 0.692). Piglet colostrum intake did not differ between control and ketoprofen groups (p = 0.736). However, the proportions of piglets that had inadequate colostrum intake were 71.3%, 22.6%, and 5.4% in those with birth weights of <1.0 kg, 1.0 to 1.29 kg, and ≥1.30 kg, respectively (p<0.001). The piglet mortality rate did not differ between control and ketoprofen groups (p = 0.808). Conclusion: Administration of ketoprofen in postpartum sows for 2 days can control the evidence of post-parturient disorders in sows as effectively as the use of tolfenamic acid. No deleterious effect of ketoprofen was detected on sow colostrum yield, piglet colostrum intake and piglet mortality. Therefore, ketoprofen can be recommended as an alternative anti-inflammatory drug used in postpartum sows.

• 환경생물
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• 제39권4호
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• pp.445-451
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• 2021

Agrobacterium tumefaciens strain CP4 유래 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) 유전자를 포함하는 유전자변형생물체(Living modified organism, LMO)가 개발되었다. 이 같은 LMO는 국내 승인되어 사료용, 식품용, 가공용으로 이용 중이다. 간이면역 검사키트 개발을 위해서는 고효율의 단클론 항체 개발이 필수적이다. 본 연구에서는 대장균 BL21 (DE3)에서 재조합 CP4 EPSPS 단백질을 정제하였으며 SDS-PAGE와 MALDI-TOF MS 분석으로 단백질 특성을 분석하였다. 단클론 항체 제작은 (주)앱클론의 SOP 매뉴얼에 따라 진행하였다. 본 연구 결과 5개의 단클론 항체 클론(2F2, 4B9, 6C11, 10A9, 10G9)를 확보하였다. 5종의 단클론 항체의 효율과 특이도 검정을 위해서 LM 면화 추출액을 이용한 western blotting 분석을 실시하였다. 모든 단클론 항체는 CP4 EPSPS를 함유하는 MON1445와 MON88913을 특이적으로 검출하였으며 비변형 면화 및 타종의 LM 면화에서는 검출되지 않았다. 이러한 결과들을 바탕으로 CP4 EPSPS 단클론 항체는 LMO에 함유된 CP4 EPSPS 단백질을 타겟으로 항체 기반 검출법 개발에 활용될 것으로 사료된다.

• Journal of Animal Science and Technology
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• 제65권5호
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• pp.951-970
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• 2023

This study utilized Italian ryegrass silage (IRGS) - based total mixed ration (TMR) as feedstuff and evaluated its effects on rumen fermentation, growth performance, blood parameters, and bacterial community in growing Hanwoo heifers. Twenty-seven Hanwoo heifers (body weight [BW], 225.11 ± 10.57 kg) were randomly allocated to three experimental diets. Heifers were fed 1 of 3 treatments as follows: TMR with oat, timothy, and alfalfa hay (CON), TMR with 19% of IRGS (L-IRGS), and TMR with 36% of IRGS (H-IRGS). Feeding high levels of IRGS (H-IRGS) and CON TMR to heifers resulted in a greater molar proportion of propionate in the rumen. The impact of different TMR diets on the BW, average daily gain, dry matter intake, and feed conversion ratio of Hanwoo heifers during the growing period did not differ (p > 0.05). Furthermore, the blood metabolites, total protein, albumin, aspartate aminotransferase, glucose, and total cholesterol of the heifers were not affected by the different TMR diets (p > 0.05). In terms of rumen bacterial community composition, 264 operational taxonomic units (OTUs) were observed across the three TMR diets with 240, 239, and 220 OTUs in CON, L-IRGS, and H-IRGS, respectively. IRGS-based diets increased the relative abundances of genera belonging to phylum Bacteroidetes but decreased the abundances of genus belonging to phylum Firmicutes compared with the control. Data showed that Bacteroidetes was the most dominant phylum, while Prevotella ruminicola was the dominant species across the three TMR groups. The relative abundance of Ruminococcus bromii in the rumen increased in heifers fed with high inclusion of IRGS in the TMR (H-IRGS TMR). The relative abundance of R. bromii in the rumen significantly increased when heifers were fed H-IRGS TMR while P. ruminicola increased in both L-IRGS and H-IRGS TMR groups. Results from the current study demonstrate that the inclusion of IRGS in the TMR is comparable with the TMR containing high-quality forage (CON). Thus, a high level of IRGS can be used as a replacement forage ingredient in TMR feeding and had a beneficial effect of possibly modulating the rumen bacterial community toward mainly propionate-producing microorganisms.

• Animal Bioscience
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• 제37권3호
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• pp.500-508
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• 2024

Objective: Due to current selection practices for increased egg production and peak persistency, the production profile, age at maturity, and body weight criteria for commercial layers are constantly changing. Body weight and age at the time of photostimulation will thus always be the factors that need to be adequately addressed among various production systems. The current study was carried out to determine the effects of pullets' body weight (low, medium, and heavy) on their performance, welfare, physiological response, and hormonal profile. Methods: With regard to live weight, 150 16-week-old pullets were divided into three groups using a completely randomized design (CRD) and held until the 50th week. One-way analysis of variance was used to evaluate the data under the CRD, and the least significant difference test was used to distinguish between treatment means. Results: In comparison to the medium and light birds, the heavy birds had higher body weight at maturity, an earlier age at maturity, and higher egg weight, eggshell weight, eggshell thickness, egg yolk index, breaking strength, egg surface area, egg shape index, egg volume, and hormonal profile except corticosterone. However, the medium and light birds had lower feed consumption rates per dozen eggs and per kilogram of egg mass than the heavy birds. Light birds showed greater body weight gain, egg production, and egg specific gravity than the other categories. At 20 weeks of age, physiological response, welfare aspects, and catalase were non-significant; however, at 50 weeks of age, all these factors-aside from catalase-were extremely significant. Conclusion: The findings of this study indicate that layers can function at lower body weights during photostimulation; hence, dietary regimens that result in lighter pullets may be preferable. Additionally, the welfare of the birds was not compromised by the lighter weight group.

• 한국습지학회지
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• 제26권3호
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• pp.219-226
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• 2024

생태계를 보호하고 생태계적 가치를 증진하기 위해 생태계서비스지불제(Payment for Ecosystem Services, PES)가 강조되고 있다. 그러나 PES 시행과 정책의 효과적인 실행을 위해 필요한 생태계 모니터링과 연구는 부족한 상황이다. 이에 본 연구는 장항습지에서 시행 중인 PES의 효과를 분석하여 서식지 관리 방안을 제시하였으며, 이때 이동성 조류 모니터링과 주요 종의 먹이원을 분석하였다. 장항습지에서 관찰된 우점 조류로는 괭이갈매기, 청둥오리, 쇠기러기 등이 있으며, 이들의 주요 먹이원은 참새귀리, 개보리 등의 벼과 식물과 속속이풀 등 십자화과 식물로 분석되었다. 또한 PES를 통해 장항습지 내에서 볍씨 공급이 이루어지고 있으며, 재두루미, 큰기러기, 쇠기러기 등의 조류가 이를 이용하고 있다는 결과가 관찰되었다. 장항습지의 지속 가능한 관리를 위해 먹이 공급 시스템 개선과 재두루미 및 기러기류(큰기러기, 쇠기러기)에 대한 분산 공급 방안을 모색해야 한다. 이때, 농업 및 개발 지역과의 경계를 관리하여 생태적 연결성을 강화해야 한다. 본 연구에서는 이동성 조류의 서식지로서 장항 습지의 생태적 중요성을 재확인하였다. 또한, 이들 결과는 향후 정책 수립과 지속 가능한 보전을 위한 기초 자료로 활용될 수 있는 의의를 갖는다.