This study investigated the growth performance and fat deposition in Woorimatdag2 (WMD2) breeder pullets in response to dietary levels of apparent metabolizable energy (AMEn). A total of 144 four-week-old WMD2 breeder pullets were weighed and equally divided into four dietary groups, each with four replications until 16 weeks of age. The dietary groups were fed diets formulated with AMEn levels; 2,600, 2,700, 2,800, and 2,900 kcal/kg. The results showed that birds fed 2,800 and 2,900 kcal/kg were higher (P<0.05) in body weight and weight gain compared to those fed 2,600 kcal/kg. Feed conversion ratio was significantly (P<0.05) improved in 2,900 kcal/kg-fed group compared to 2,600 and 2,700 kcal/kg-fed groups. The laying ages of first egg were earlier (P<0.05) in 2,600 and 2,700 kcal/kg-fed pullets than 2,900 kcal/kg-fed those. Meanwhile, the higher (P<0.05) fat (%) into both liver and abdomen was confirmed in groups fed 2,700, 2,800, and 2,900 kcal/kg compared to group fed 2,600 kcal/kg. Furthermore, blood concentrations of AST and ALT were significantly (P<0.05) increased in group fed 2,900 kcal/kg compared to groups fed 2,600, 2,700, and 2,800 kcal/kg. Accordingly, we suggest that 2,600 kcal AMEn/kg is appropriate for WMD2 breeders during growing phase, which may improve their productivity and health of those during laying phase.