• 한국수산과학회지
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• 제42권5호
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• pp.442-448
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• 2009

Bacteriological examination of a 12,700 ha area within Jaranman Saryangdo area, located in Jaran bay and Saryangdo island, was conducted with 35 fixed (designated areas and adjacent areas) seawater sampling stations between January 2006 and December 2008. According to results, the geometric mean range and the estimated 90th percentile range of fecal coliform counts in sea water samples collected in the designated area were <1.8-2.4 and <1.8-8.6 MPN/100mL, respectively. The estimated 90th percentile range of fecal coliform counts in sea water samples collected from 6 sampling stations in the adjacent areas were 6.1-34.6 MPN/100mL. Based on these results, bacteriological water quality of the designated areas in the Jaranman. Saryangdo area meet the NSSP (National Shellfish Sanitation Program) guidelines for approved area. The bacteriological sea water quality in Jaranman.Saryangdo area has been shown to be favorable at all investigated sampling stations except for July and August which coincided with heavy rainfall. In fact, the bacteriological water quality was not affected if rainfall was less than 30 mm rainfall. However, the degree of bacteriological contamination increased rapidly and the water quality exceeded approved NSSP guidelines set for rainfall above 60 mm.

• Parasites, Hosts and Diseases
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• 제32권1호
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• pp.27-34
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• 1994

지난 1984년의 제1차 보고에 이어 1984년부터 1992년까지 9년간 서울 백병원 외래 및 입원환자 중 기생충검사를 의뢰한 52.552명에 대하여 포르말린-에테르 집란법 및 직접 도말법으로 대변검사를 시행하였던 바 그 결과는 다음과 같았다. 총 윤충류(13종) 충란 및 원충류(5종) 포낭 양성률은 각각 평균 6.5% 및 2.5%이었다. 윤충류 종별 양성률(양성자수)은 간흡충 3.2% (1.667). 편충 2.1%(1 089) 요꼬가와흡충 1 2%(613), 회충 0.2%(100) 동양모양선충 0.06%(34). 유무구조충 0.05%(28). 왜소조충 0.03%(18). 구충 0.03%(17). 극구흡충류 0.03%(15), 폐흠충 0.02%(12). 요충 0.02%(10) 분선충 0.01%(6), 광절열두조충 0.004% (2)이었다. 원충 별로는 대장아메바 1.1%(588). 왜소아메바 0.8%(402), 람블편모충 0.3% (173), 이질아메바 0.3%(164). 장트리코모나스 0.004%(2)로 나타났다. 조사년도별로 볼 때 회 충 및 편충 등 토양매개성 윤충은 뚜렷한 감소를 보이는 반면 간흡충을 비롯한 패류 매개성 윤충과 장내 원충류는 양성률의 감소를 인정할 수 없었다.

• 한국임상수의학회지
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• 제27권1호
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• pp.1-5
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• 2010

미포자충인 Enterocytozoon bieneusi는 최근에 AIDS 환자에 기회감염 되는 병원체로 부각되었다. 이 병원체는 여러 동물에서 발견되고 있으며 인수공통 기생충으로서 특별한 관심의 대상이 되고 있다. 충남, 대전, 전북 지역의 도축장으로부터 특별한 증상을 보이지 않은 건강한 한우 총 1,729 마리의 직장변을 취하여 E. bieneusi의 감염율을 조사하였다. 조사방법은 Trichrome 염색 변법과 중합효소연쇄반응(PCR)을 이용한 분자생물학적 기법을 적용하였다. Trichrome 염색변법을 적용한 광학현미경 관찰에서 194 (11.28%) 개체에서 미포자충이 관찰되었으며, 특히 3월에 가장 높은 감염율을 보였다. 한편 분자생물학적 기법을 적용한 시험에서는 79 (4.59%) 개체에서 양성 반응을 보였으며, 이 역시 3월에 가장 높은 감염율을 나타내었다.

• Journal of Microbiology and Biotechnology
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• 제27권11호
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• pp.1971-1982
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• 2017

Piliated Lactobacillus rhamnosus (pLR) strains possess higher adherent capacity than non-piliated strains. The objective of this study was to isolate and characterize probiotic pLR strains in human fecal samples. To this end, mouse polyclonal antiserum (anti-SpaA) against the recombinant pilus protein (SpaA) of L. rhamnosus strain GG (LGG) was prepared and tested for its reactivity and specificity. With the anti-SpaA, a method combining the de Man, Rogosa, and Sharpe (MRS) agar plating separation and colony immunoblotting (CIB) was developed to isolate pLR from 124 human fecal samples. The genetic and phenotypic characteristics of the resultant pLR isolates were compared by randomly amplified polymorphic DNA (RAPD) fingerprinting, and examination of adhesion to Caco-2 cells, hydrophobicity, autoaggregation, and in vitro gastrointestinal tolerance. Anti-SpaA specifically reacted with three pLR strains of 25 test strains, as assessed by western blotting, immunofluorescence flow cytometry, and immunoelectron microscopy (IEM) assays. The optimized MRS agar separation plus anti-SpaA-based CIB procedure could quantitatively detect $2.5{\times}10^3CFU/ml$ of pLR colonies spiked in $10^6CFU/ml$ of background bacteria. Eight pLR strains were identified in 124 human fecal samples, and were confirmed by 16S RNA gene sequencing and IEM identification. RAPD fingerprinting of the pLR strains revealed seven different patterns, of which only two isolates from infants showed the same RAPD profiles with LGG. Strain PLR06 was obtained with high adhesion and autoaggregation activities, hydrophobicity, and gastrointestinal tolerance. Anti-SpaA-based CIB is a rapid and inexpensive method for the preliminary screening of novel adherent L. rhamnosus strains for commercial purposes.

• Journal of Trauma and Injury
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• 제29권4호
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• pp.201-203
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• 2016

Rectal injury is seen in 1-2% of all pelvic fractures, and lower urinary tract injury occurs in up to 7%. These injuries are rare, but if missed, can lead to a severe septic response. Rectal injury may be suspected by the presence of gross blood on digital rectal examination. However, this classic sign is not always present on physical examination. If an Antero-Posterior Compression type pelvic fracture is seen, we should consider the possibility of rectal and lower urinary tract injury. It is important to define the anatomic location of the rectal injury as it relates to the peritoneal reflection. Trauma to the intraperitoneal rectum should be managed as a colonic injury. Extraperitoneal rectal injury should be managed with fecal diversion regardless of primary repair. We present the case of a 46-year-old man who was referred to our hospital following a major trauma to the pelvis in a pedestrian accident.

• 한국임상수의학회지
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• 제28권3호
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• pp.307-309
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• 2011

1년령 암컷 요크셔테리어가 설사와 활력저하로 충북대학교 동물의료센터에 내원하였다. 혈액, 혈청화학 및 방사선 검사 결과로 환자는 복막염을 동반한 자궁축농증으로 진단하였다. 설사 원인 진단을 위한 분변 검사에서는 다수의 효모 증식이 관찰되었으며, 진균 배양 및 분자생물학적 동정으로 C. parapsilosis를 최종적으로 확인하였다. 이 보고는 개에서 C. albicans외에 C. parapsilosis도 장관 질환을 야기할 수 있음을 보여준다.

• Parasites, Hosts and Diseases
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• 제57권3호
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• pp.243-248
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• 2019

The purpose of this study was 2-fold: 1) to investigate the prevalence of gastrointestinal parasite infection in cats reared in Daegu, Republic of Korea and 2) to assess the efficacy and safety of a topical emodepside/praziquantel formulation for cats with parasitic infections. The gastrointestinal parasite infections were examined microscopically using the flotation method. Of 407 cats, 162 (39.8%) were infected by at least one gastrointestinal parasite, including Toxocara cati (63.0%), Toxascaris leonina (31.5%), Taenia taeniaeformis (3.7%), and Cystoisospora felis (1.9%). None of the infected animals had multiple infections. When the data were analyzed according to sex, age, and type of cat, stray cats showed statistically higher prevalence than companion cats (P<0.05). On the 5th day after treatment, no parasitic eggs were detected using microscopic examination. In addition, no adverse effects, such as abnormal behaviors and clinical symptoms, were observed in the cats treated with the drug. These results quantify the prevalence of gastrointestinal parasites in cats in Daegu, Republic of Korea, and show that topical emodepside/praziquantel is a safe and effective choice for treating the parasitic infections in cats.

• Asian Pacific Journal of Cancer Prevention
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• 제14권3호
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• pp.1969-1974
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• 2013

The objective of this study was to determine the knowledge and associated factors regarding colorectal cancer screening among university students in Malaysia. The questionnaire consisted of three parts: socio-demographic characteristics, lifestyle practice and knowledge of colorectal screening. A cross-sectional study was conducted among 300 students ($21.3{\pm}1.4$ years old). The majority of the participants were Malay with a monthly family income of less than 5,000 Ringgit Malaysia (equal to 1,700 USD) (67.0% and 76.0%, respectively). Regarding their lifestyle practices, the majority were non-smokers and had never consumed alcohol (83.7%, and 88.0%, respectively). The majority of the participants had no knowledge of digital rectal examination, colonoscopy, barium enema and fecal occult blood screening (63.3%, 60.7%, 74.0% and 62.3%, respectively). Univariate and multivariate analysis revealed that their age and the discipline which the students were studying significantly influenced their level of knowledge about colorectal screening. The present study results indicate that education campaigns about colorectal cancer should be promoted.

• Biomolecules & Therapeutics
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• 제1권1호
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• pp.109-120
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• 1993

Mechanisms for the hypocholesterolemic effetcs of $\beta$-glucan remain unclear. Rats were divided into 3 groups; normal control group, atherogenic group(oral administration of cholesterol 40 mg/kg/day and vitamin $D_2$320,000 IU/kg/day),${\beta}$-glucan treatment group(oral administration of atherogenic treatment and ${\beta}$-glucan 0.135 g/kg/day). The ${\beta}$-glucan treatment group showed moderate increases of serum lipids concentration compared with atherogenic group. In histopathological examination, aortas showed no critical lesions. The total fecal neutral sterols and bile acids excreted for 6 days was increased compared with both normal and atherogenic group. These result\ulcorner suggest that mechanisms for the hypocholesterolemic effect of ${\beta}$-glucan on rats were due to the inhibition of cholesterol absorption in the intestinal lumen and acceleration of cholesterol catabolism in the liver.

• 한국동물위생학회지
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• 제18권2호
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• pp.152-157
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• 1995

A total of 1050 layer and broiler chickens from 63 flocks of 21 poultry farms in South-Kyeonggi area, aged from 1 to 18 weeks, were investigated for the prevalence of Cryptosporidium baileyi infection from May 1992 to March 1993. The results have shown that 44 chickens(4.19%) were infected with C.(baileyi during the period of investigation) Fecal samples were treated by using Sheather's flotating technique and were examined under the light and phase contrast microscopy and then stained by Kinyoun method. Cryptosporidium oocysts were found in 35 out of 650 broilers(5.4%) and 9 out of 400 layer chickens(2.3%) aged mainly from 2 to 12 weeks. The regional infection rate were 4.7% in Pyeong-taek, 5.1% in An-sung and 2.3% in Yong-in, respectively. The average size of isolated oocyst was about $5.23{\times}4.92{\mu}m$ and the oocysts were orally inoculated into 2-day-old SPF chickens for the histological examination of oocyst in bursa of Fabricius. The study has concluded that C. baileyi infects chickens and oocysts were isolated in South-Kyeonggi area.