• 농업과학연구
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• 제48권3호
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• pp.447-454
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• 2021

This study was conducted to evaluate the effects of different copper sources (inorganic and organic) on the growth performance, fecal copper excretion, intestinal morphology, and health in growing pigs. A total of 40 growing pigs (30.22 ± 1.92 kg) were randomly assigned to 5 dietary treatments: a basal control diet (CON), 4 experimental diets supplemented with either copper sulfate (CuSO₄), Cu-glycine complex (CuGly), Cu-amino acid complex (CuAA), or Cu-hydroxy-4-methylthio butanoate chelate complex (CuHMB) at 100 ppm, respectively. At the end of the study (28 days), fecal and blood samples were collected, and the pigs were slaughtered to determine the intestinal morphology. During the 28 days of the experimental period, pigs fed the inorganic and organic copper showed a higher average daily gain (p < 0.01) and gain feed ratio (p < 0.01). There were no differences in mineral concentrations of the serum; however, the copper concentration of the feces was lower (p < 0.01) in the CuAA and CuHMB groups. The intestinal morphology and blood profiles did not significantly differ between the groups. In conclusion, the organic copper sources (CuAA and CuHMB) can be used as a growth promoter to replace the CuSO₄ without any negative effects on health in growing pigs and to reduce fecal copper excretion.

• Journal of Nutrition and Health
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• 제30권3호
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• pp.229-243
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• 1997

This study was performed to investigate the effects of dietary fibers from tangerine peels on lipid and cadmium metabolism. And effects were compared with those of commercial dietary fibers($\alpha$-cellulose, citrus pectin). Sixty male rats of Sprague-Dawley strain weighing 186.7$\pm$2.6g were blocked into 12 groups according to body weight, and were raised for 2 weeks. Cadmium chloride was given at the levels of 0 of 400 ppm in diet. Various dietary fibers were given at the level of 0 or 4%(w/w) of diet. The results are summarized as follow. In lipid metabolism, insoluble fibers[insoluble dietary fibers from tangerine peels(IDE), $\alpha$-celluolse] increased fecal excretion of lipids by inhcreasing feces weight, and decreased the concentrations of serum triglyceride and liver lipids. Soluble dietary fibers from tangerine peels(SDF) decreased the concentrations of serum cholesterol and liver lipids by increasing fecal lipids, too. In cadmium metabolism, soluble fivers(SDF, pectin) inhibited Cd absorption by increasing fecal Cd excretion and decreased Cd concentrations of intestion, liver and kidney. In conclusion, among the extracted fibers, SDF were more effective on lipid and Cd lowering activity and IDF had effect of increasing fecal lipid excretion. This result is useful to reduce food waste and utilize waste products.

• 한국가금학회지
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• 제21권2호
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• pp.133-138
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• 1994

A study of concurrent bioassay for protein quality and energy level in protein sources was rnade by determining urinary nitrogenous compounds in excreta. The carry over effect of previous feeding was eliminated by 48 h of feeding the experimental diets prior to the determination of for protein digestibility and utilizability, and energy digestibility and metabolizability at 24 h interval during 3 days. Then, protein qualities and energy levels for soybean meal, rapeseed meal and fish meal were calculated by a substitution method. Apparent protein utilization (NB/NI) was affected by the increased fecal nitrogen excretion in soybean meal and by the increased urinary nitrogen excretion in rapeseed meal and fish meal. The apparent metabolizability of energy (ME/GE) was affected by the fecal energy excretion in soybean meal and rapeseed meal and by urinary energy excretion in fishmeal. The results indicated that the concurrent bioassay of protein quality and energy levels in ingredients appears to be applicable to chickens of other age, sex, breeds and environmental conditions.

• 한국식품영양과학회지
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• 제31권5호
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• pp.819-825
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• 2002

본 연구는 메탄올과 물에서 각각 추출한 다시마 추출물을 급여한 흰쥐에게 당뇨를 유도하고 다시마 추출물의 섭취가 당뇨병의 혈관계 합병증의 유발과 관련되는 체내 지질대사의 변화에 미치는 영향을 조사하고자 하였다. 흰쥐에게 다시마의 메탄올추출물(2%, w/w)과 물추출물(4%, w/w)을 식이에 첨가하여 4주간 급여하고, streptozotocin(STZ, 45 mg/kg BW)으로 당뇨를 유도한 후 혈당, 혈중 콜레스테롤과 중성지질, 변의 수분 함량과 중량, 변의 스테로이드 및 담즙산 함량을 측정하였다. 당뇨 유발로 인해 흰쥐의 체중 감소가 현저하였으며 식 이에 따른 유의적인 차이는 나타나지 않았지만 메탄올추출물 급여군보다 물추출물 급여군에서 당뇨에 의한 체중감소가 적은 것으로 나타났다. 식이에 따른 식이효율의 차이는 나타나지 않았으나 식이 섭취량은 물추출물 급여군이 유의적으로 낮은 경향이었다. 혈당 변화는 당뇨를 유도하고 다시마추출물을 급여하지 않은 대조군에 비해 다시마추출물 급여군에서 유의적으로 감소하였으나 추출물의 종류에 따른 차이는 나타내지 않았다. 중성지방 함량은 대조군에 비해 다시마추출물을 급여한 군에서 중성 지질 함량이 유의적으로 감소하는 경향을 나타내었으나 추출물간의 차이는 관찰되지 않았다. 인지질 함량은 다시마추출물 급여군에 비해 대조군에서 약간 높았으나 메탄올추출물과 물추출물간의 차이는 관찰되지 않았다. 혈장에서의 총 콜레스테롤 함량은 실험군간의 차이를 보이지 않았다 HDL-콜레스테롤 함량은 실험군간의 유의적인 차이는 없으나 대조군과 메탄올추출물 급여군에 비해 물추출물 급여군에서 그 함량이 높았다 분변의 건조중량은 다시마의 물추출물 급여군에서 유의적으로 증가하였다. 분변 중의 수분 함량은 유의적이지는 않지만 대조군이나 메탄올추출물 급여군보다 물추출물 급여군에서 증가하는 경향이었다. 변으로 배설되는 콜레스테롤 함량은 메탄올과 물에서 각각 추출한 다시마 추출물 급여군이 대조군에 비해 유의적으로 높았으며, 특히 물추출물 급여군에서 가장 많이 배설되었다. 하루에 변으로 배설되는 coprostanol함량은 다시마의 물추출물 급여군에서 유의적으로 증가되었다 변으로 배설되는 담즙산의 함량은 메탄올과 물추출물 급여군에서 대조군에 비해 유의적으로 높았다 이상과 같은 결과를 통해서 볼 때 다시마추출물 섭취는 콜레스테롤과 같은 중성스테로이드의 배설을 촉진시킴으로써 당뇨로 인해 발생하기 쉬운 합병증인 동맥 경화증의 예방적 효과에 기여할 수 있으리라 사료된다.

• 한국응용과학기술학회지
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• 제3권1호
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• pp.49-55
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• 1986

The purpose of the study was to find an effect of Medium Chain Triglycerids (MCT) diet on cholesterol metabolism in rat. Sprague-Dawley rats were fed two different diets containing MCT(trioctanoate) and corn oil respectively. After feeding to each group for four weeks, the levels of serum and liver cholesterol, the excretion rates of fecal and biliary steroids, and also bile acid composition were investigated. The results obtained from the study are as follows : (1) The average body weight gain in MCT group was almost same as that in the corn oil group. (2) The concentration of serum cholesterol in MCT group was lower than that in the corn oil group. Therefore it is confirmed that the cholesterol lowing action of MCT diet was practically high. (3) The concentrations of liver cholesterol and Triglyceride in MCT group were almost same as that in the corn oil group. Therefore it is thought that the level of liver lipids was not influenced by the difference of diet in this study. (4) The excretion rate of fecal neutral steroid in MCT group was significantly lower than that in the corn oil group, while the rate of fecal bile acid excretion was about same in both MCT and corn oil group. (5) The composition rates of fecal bile acid such as cholic acid, chenodeoxycholic acid and deoxycholic acid, a secondary acid of cholic acid, in MCT group were significantly lower than that in the corn oil group. (6) The excretion rates of biliary cholesterol and bile acid in MCT group were significantly higher than that in the corn oil group, while the composition rates of biliary bile acid such as chenoddeoxycholic acid and deoxycholic acid in MCT group were significantly higher than that in the corn oil group.

• Journal of Nutrition and Health
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• 제26권1호
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• pp.13-20
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• 1993

To investigate the effects mustard leaf(Brassica Juncea) on Cholesterol metabolism, male Sprague Dawley rate were fed semipurified diets containing 2% or 4% mustard leaf with or without cholesterol for 5 weeks. Plasma cholesterol content decreased significantly by feeding 4% mustard leaf with of without cholesterol for 5 weeks. Plasma cholesterol content decreased significantly by feeding 4% mustard leaf in rats fed 1% cholesterol in the diet. In addition, HDL-cholesterol increased slightly by the feeding of mustard leaf, resulting in a significant increase in the HDL-cholesterol/total cholesterol ratio and a reduction of atherosclerotic index. However, levels of plasma lipids were not influenced by mustared leaf in rats fed cholesterol-free diet. The contents of all classes of lipid in liver increased by dietary cholesterol. Of the liver lipids, triglyceride and cholesterol ester were accumulated most, showing a fatty liver synodrome. Supplementation of mustard leaf to cholesterol-containing diet resulted in a slight decrease in neutral lipid contents of liver. Fecal cholesterol excretion was higher by more than 2.7 and 3.3-fold in rats fed 2 and 4% mustard leaf than in control rats fed cholesterol. Similar trends were found in fecal bile salt excretion; rats fed and 4% mustard leaf excreted more bile salts by more than 1.5 and 2% than those fed control diet containing cholesterol. In summary, mustard leaf may have an antiatherogenci effect of reducing plasma cholesterol level and increasing HDL-cholesterol level. The plasma cholesterol lowering effect of mustard leaf is suggested to be due, at least in part, to increase in fecal excretion of cholesterol and bile acids.

• 대한지역사회영양학회지
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• 제2권4호
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• pp.523-529
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• 1997

This study was conducted to obtain accurate data on the intake, digestibility and nitrogen balance of protein in Korean college women. Subjects were 8 female college students, aged from 21 to 23, and maintained their menu and life patterns regular during a 4- week study. The same amount of diet that the subjects had consumed, and feces and urine were collected and measured to extract their nitrogen content by Kjeldahl method. From this data, apparent digestibility and the body nitrogen balance were estimated by determing daily protein intake and excretion. The daily protein intake was 56.9$\pm$1.4g and daily fecal protein loss was 6.3$\pm$0.2g. The apparent digestibility of protein was 89.6$\pm$0.7$\%$. The daily nitrogen intake measured by Kjeldahl method was 9.43$\pm$0.2g. The urinary nitrogen excretion was 7.64$\pm$0.23g and fecal nitrogen excretion was 1.02$\pm$0.03g. The nitrogen balance indicated a positive balance of 0.45$\pm$0.18g. (Korean J Community Nutrition 2(4) : 523-529, 1997)

• Journal of Nutrition and Health
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• 제37권5호
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• pp.352-363
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• 2004

Lipid-lowering effects of the inulin have been demonstrated in animal, yet attempts to reproduce similar effects in humans have generated conflicting results. In this study, the lipid-lowering potential of inulin and especially its effect on bile acid and neutral sterol excretion were investigated in Korean postmenopausal women. Nineteen postmenopausal women were randomly divided into two groups in a double-blind parallel design and consumed one of two supplements for 12 weeks; placebo of 8g maltodextrins/sucrose mixture (placebo group) or 8g inulin (inulin group). There were no significant changes in body weight during the supplementation period in either inulin or placebo group. Dietary consumption of animal fat in both group tended to decrease after 12 weeks of experiment. Intake of cholesterol was lower in placebo group, whereas the decrease of cholesterol intake in inulin group did not reach statistical significance after 12 weeks. The levels of serum total cholesterol (TC) and LDL-cholesterol (LDL-C) were significantly decreased in both placebo (p＜0.05) and inulin group (p＜0.01) after supplementation for 12 weeks compared with the baseline. The levels of serum triglyceride (TG) and HDL-cholesterol (HDL-C) were not significantly affected by inulin supplements, but atherogenic index (AI) and LDL-C/HDL-C ratio (LHR) as a predictor for coronary heart disease were improved (p＜0.01) significantly after inulin supplementation. Therefore, inulin supplement may decrease the risk of cardiovascular disease via improving blood cholesterol level. Fecal weight and pH were not changed after 12 weeks of supplementation. There were no statistically significant changes for the fecal short-chain fatty acids (SCFAs). In inulin group, fecal deoxycholic acid (DCA) was significantly lowered compared with the baseline (p＜0.05) whereas other bile acids were not changed. During the 12 weeks of intervention, no differences were found in fecal excretion of neutral sterol in the two groups. In summary, dietary inulin decreases serum TC, LDL-C, AI, LHR and lowers excretion of fecal DCA in the Korean postmenopausal women. These results support the use of inulin for reducing risk factors for hyperlipidemic postmenopausal women. However, the exact mechanism (s) responsible for the blood lipid lowering action of inulin including altered fecal bile acid remain to be elucidated.

• Journal of Applied Biological Chemistry
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• 제44권3호
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• pp.131-134
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• 2001

Effects of water-soluble extract from roasted-chicory root on the cholesterol metabolism in rats fed cholesterol diet were investigated. Sprague-Dawley rats received a hypercholesterolemic diets without (control group) or with 5.0% water-soluble extract from roasted chicory root for 2 weeks. Roasted chicory extract group showed significantly higher body weight gain and food intake compared with the control group. The concentrations of total cholesterol and LDL+VLDL cholesterol in serum were significantly lower in rats fed roasted chicory extract diet. However, HDL-cholesterol concentration, and atherogenic index were not significantly affected by the dietary roasted chicory extract. Fecal net weight, fecal cholesterol, and bile acid excretion were significantly higher in the chicory extract group. The results suggest that the hypocholesterolemic effect in rats fed roasted chicory extract may be caused by an alteration in the absorption of cholesterol by an increase in the fecal excretion of cholesterol and bile acid.

• 한국식품영양과학회지
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• 제24권6호
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• pp.978-983
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• 1995

The effect of sulfur-containing amino acids on lipid metabolism was studied in rats fed casein as a protein source. Plasma cholesterol, HDL-cholesterol and atherosclerotic index decreased in the cysteine group compared to the methionine group. Plasma triglyceride and phospholipid level were not affected by the supplementation of the sulfur-containing amino acids. The levels of cholesterol and triglyceride in liver decreased by both methionine and cysteine. Cysteine increased the fecal excretion of coprostanol, total neutral steroid and bile acid. The results suggest that plasma cholesterol level is affected by dietary ratio of cysteine/methionine and that the hypocholesterolemic effects of cysteine is, at least in part, through reducing cholesterol absorption from small intestine and through enhancing fecal excretion of bile acids.