• Journal of Applied Biological Chemistry
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• 제48권2호
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• pp.75-78
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• 2005

Halotolerant protease produced by Halomonas marisflava KCCM 10457 was partially purified through ammonium sulfate precipitation and Sephacryl S-200HR gel permeation chromatography. Optimal pH and temperature of protease were 11.0 and $45^{\circ}C$, respectively. Enzyme activity was inhibited by $Cu^{2+}$, $Hg^{2+}$, $Fe^{2+}$, and $Fe^{3+}$, and selectively inhibited by p-chloromercuribenzoic acid (PCMB), suggesting this enzyme is cysteine protease. The enzyme is halotolerant, because it retained 77% of original activity in presence of 3.33 M NaCl. The protease showed broad substrate specificity to various natural proteins; BSA, casein, egg albumin, gelatin, and hemoglobin.

• Asian-Australasian Journal of Animal Sciences
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• 제10권3호
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• pp.298-303
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• 1997

Sixteen holstein heifers were employed to study the effect of thermal stress on rectal temperature and mineral status of heifers around parturition during hot and cool weather. During the hot weather, rectal temperatures of the heifers measured at 08:30 and 15:30 h from 7 d prepartum to parturition were higher than those during the cool weather. Blood hematocrit and hemoglobin were not significantly different between the hot and cool weather, but at 2 and 1 wk prepartum, those during the hot weather were higher. Plasma Ca, inorganic P and Fe of the heifers during the hot weather were higher than those during the cool weather, but plasma Mg and Zn and serum alkaline phosphatase during the hot weather were lower. Colostral protein and Mg of heifers at parturition during the hot weather were lower than those during the cool weather, but colostral Ca, P, Fe and Zn were not affected by the weather.

• Nutrition Research and Practice
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• 제9권6호
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• pp.613-618
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• 2015

BACKGROUND/OBJECTIVES: Iron deficiency in early life is associated with developmental problems, which may persist until later in life. The question of whether iron repletion after developmental iron deficiency could restore iron homeostasis is not well characterized. In the present study, we investigated the changes of iron transporters after iron depletion during the gestational-neonatal period and iron repletion during the post-weaning period. MATERIALS/METHODS: Pregnant rats were provided iron-deficient (< 6 ppm Fe) or control (36 ppm Fe) diets from gestational day 2. At weaning, pups from iron-deficient dams were fed either iron-deficient (ID group) or control (IDR group) diets for 4 week. Pups from control dams were continued to be fed with the control diet throughout the study period (CON). RESULTS: Compared to the CON, ID rats had significantly lower hemoglobin and hematocrits in the blood and significantly lower tissue iron in the liver and spleen. Hepatic hepcidin and BMP6 mRNA levels were also strongly down-regulated in the ID group. Developmental iron deficiency significantly increased iron transporters divalent metal transporter 1 (DMT1) and ferroportin (FPN) in the duodenum, but decreased DMT1 in the liver. Dietary iron repletion restored the levels of hemoglobin and hematocrit to a normal range, but the tissue iron levels and hepatic hepcidin mRNA levels were significantly lower than those in the CON group. Both FPN and DMT1 protein levels in the liver and in the duodenum were not different between the IDR and the CON. By contrast, DMT1 in the spleen was significantly lower in the IDR, compared to the CON. The splenic FPN was also decreased in the IDR more than in the CON, although the difference did not reach statistical significance. CONCLUSIONS: Our findings demonstrate that iron transporter proteins in the duodenum, liver and spleen are differentially regulated during developmental iron deficiency. Also, post-weaning iron repletion efficiently restores iron transporters in the duodenum and the liver but not in the spleen, which suggests that early-life iron deficiency may cause long term abnormalities in iron recycling from the spleen.

• 대한지역사회영양학회지
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• 제2권1호
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• pp.23-32
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• 1997

To evaluate iron nutritional status of female college students, fasting blood samples were taken from 76 female students of Kangnung National University. Hemoglobin(Hb), hematocrit(Hct), serum iron(Fe), total iron binding capacity(TIBC) and serum ferritin concentrations were measured and transferrin saturation was calculated. Mean values for Hb, Hct, Fe, TIBC, TS and serum freeitin were 13.64$\pm$1.42g/dl, 40.99$\pm$4.31%, 103.0$\pm$33.3$\mu\textrm{g}$/이, 395.3$\pm$9.07$\mu\textrm{g}$/dl, 26.58$\pm$9.07$\%$and 26.76$\pm$17.5ng/ml, respectively. Prevalence of iron deficiency greatly varied by indices from 6.8% when judged by Hct to 26.0$\%$ by serum ferritin concentration. The Hb concentration was positively correlated with hematocrit (r=0.5402), serum iron(r=0.2819) and transferrin saturation(r=0.2777)(p<0.05). on the other hand, serum ferritin concentration showed significantly negative correlation with TIBC(r=-0.3196). Two-day dietary intake records were collected from subjects to estimate mean daily iron intake and bioavailability of dietary iron. Mean daily intake of iron was 13.15mg and heme iron intake was 0.83mg which was 6.4% of total iron intake. Total absorbable iron calculated by the method of Monsen was 1.27mg and bioavailability of dietary iron was 9.6%. In the light of high prevalence of iron deficiency based of serum ferritin concentration and low bioavailability of iron in the diet, guidelines about diet should be made to increase the content and bioavailability of iron in the diet if female college students.

• Preventive Nutrition and Food Science
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• 제8권4호
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• pp.406-411
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• 2003

The objective of this study was to investigate the effects of iron supplementation and nutrition education on the iron status and anemia of middle school girls in Ulsan city in Korea. The subjects were already diagnosed as having anemia (hemoglobin < 12 g/dL) or iron deficiency (ferritin < 12 ng/mL and/or transferrin sataturation < 14 %). Over a period of three months, one iron tablet (80 mg Fe as ferrous sulfate/day) was administered to the iron deficient subjects and two tablets (160 mg Fe as ferrous sulfate/day) were administered to the anemia subjects. Total calorie intake of subjects was 82.1 % of RDA. The iron intake of subjects was 91.3 % of RDA and the Ca intake was 78.8 % of RDA. The basal hemoglobin concentration of subjects averaged 12.8 1.2 g/dL, and this increased significantly (p < 0.001) to 13.2 0.9 g/dL after iron supplementation. The basal ferritin concentrations were 14.9 14.2 ng/mL and these significantly increased to 26.6 19.8 ng/mL (p < 0.001). The level of total iron binding protein (TIBC) significantly decreased from the initial 523.1 108.7 $\mu\textrm{g}$/dL to 462.2 90.2 $\mu\textrm{g}$/dL (p < 0.001) after iron supplementation. Anemia symptoms such as‘Being bruised easily’, ‘Inflamed inner mouth’, and ‘Pale face’ improved significantly after iron supplementation in the subjects. There was a negative correlation between their class & year ranking and serum iron level, transferrin saturation after nutritional education and iron supplementation. It was shown, therefore, that the higher the improvement of their anemia level after iron supplementation, the higher their academic performance. It was shown that there was some improvement of their dietary attitudes after nutritional education, and that their serum level related to anemia symptoms and iron nutrition was improved after iron supplementation.

• 한국식품영양과학회지
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• 제18권3호
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• pp.348-355
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• 1989

Alkaline protease 생성능이 강한 Aspergillus fumigatus 균주를 토양에서 분리하고, 생성효소를 정제하여 특성을 조사한 결과 최적 pH는 9.0, pH안정성은 $pH\;8.0{\sim}10.0$, 최적온도는 $50^{\circ}C$였으며, $50^{\circ}C$이하의 온도에서 안정하나 그 이상의 온도에서는 급격한 효소 불활성화를 보였고, 금속염 $Mn^{++},\;Cu^{++},\;Ba^{++},\;Mg^{++}$ 등에 의해서 활성이 다소 증대되나 $K^+,\;Fe^{+++},\;Ag^{++},\;Pb^{++},\;Na^+,\;Ca^{++},\;Hg^+,\;Zn^{++}$에 의해 저해를 받았다. 활성저해제인 EDTA, 2,4-DNP, ${\varepsilon}-amino$ caproic acid에는 큰 저해를 받지 않으나, PCMB에 많은 저해를 받는 것으로 미루어 활성 부위가 SH기인 cystein protease로 추정되었다. Km값은 $8.33{\times}10^{-4}mole/{\ell}$, Vmax는 $47.62{\mu}g/min$였으며, casein과 hemoglobin을 trypsin보다 더 잘 분해하고, casein을 hemoglobin보다 잘 분해하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제14권5호
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• pp.640-645
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• 2001

In order to evaluate the change in plasma and fecal mineral content of calves to 6 days of age, and to clarify the relationship between electrolyte status and fecal consistency in calves at 6 days of age, data were collected from 52 Holstein calves. Fecal DM and Na of calves decreased at 6 days of age, but fecal Ca, P, Mg, K, Fe and Zn increased. Plasma Ca, inorganic P, Mg, Na, and Fe as well as blood hematocrit and hemoglobin of calves decreased at 6 days of age, but plasma alkaline phosphatase increased. Colostral Ca, P, Mg, Fe and Zn decreased with time postpartum, but colostral Na and K were not affected. The high plasma Na and K of calves at 6 days of age were influenced by the values at birth. There were negative correlations between fecal DM and fecal Na, and fecal K, of calves at 6 days of age, negative correlations between fecal DM and plasma Na, with plasma K. Plasma and fecal Na as well as plasma and fecal K of calves at 6 days of age were increased by the occurrence of diarrhea. These results suggest that the electrolyte status of calves at 6 days of age is adversely affected by the occurrence of diarrhea, and high plasma K and Na of calves at 6 days of age may be partly due to the electolyte status of calves at birth.

• 대한수의학회지
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• 제39권4호
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• pp.679-688
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• 1999

The combined effects of iron and selenium status on glutathione peroxidase (GSHPx) activity, cytochrome P-450 activity, and lipid peroxidation in the liver and intestinal mucosa of rats were investigated. In experiment one, four experimental groups (+Se+Fe, -Se+Fe, +Se++Fe, -Se++Fe) were manipulated for 3 weeks with intramuscular administration of irondextran (++Fe) and/or normal diet (+Fe) and deionized water (-Se) and/or selenium-supplemented deionized water (+Se). In experiment two, 2% dietary carbonyl iron (instead of the parenteral administration) was fed for 3 weeks to rats. Body weight of rats was significantly decreased in both parenterally and orally iron-overloaded groups (p<0.01), regardless of Se supplement. Serum iron was significantly increased in parenterally iron-overloaded groups but it was marginally increased in orally iron-overloaded groups. There was no significant difference in hemoglobin content among experimental groups in either experiment one or two. Total iron in the small intestine, intestinal mucosa, and livers was significantly high in both parenterally and orally iron-overloaded rats, regardless of selenium status. In the liver and intestine, GSHPx activity was significantly higher in all selenium-supplemented groups, compared to Se-deficient groups (p<0.01) and lipid peroxidation was significantly enhanced in both parenterally and orally iron-overloaded groups, compared to iron-adequate groups. There was no significant difference in cytochrome P-450 activity in the livers between groups in both experiment one and two. These results indicated that GSHPx activity in liver and intestinal mucosa was depended on selenium status, regardless of iron status, and iron-overload enhances lipid peroxidation in liver and intestinal mucosa by increasing the tissue iron content.

• 대한지역사회영양학회지
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• 제7권3호
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• pp.316-326
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• 2002

This study was carried out on 178 bus drivers in the Masan area to investigate their health and nutritional status. THe nutritional status of the subjects was surveyed using a self-administered questionnaire, and blood analyses were conducted through annual health examinations in June, 1999. The results were as fellows: About 51% were classified as normal, 33% as health-concerned, and 17% as disease-suspected. The levels of total seam cholesterol were significantly higher in the over 20 year group (194.64 $\pm$ 41.42 mg/dl) than in the under 10 year group (175,00 $\pm$ 31.33 mg/dl) with respect to the length of their driving careers. Systolic blood pressure (SBP) was significantly higher in the 51 to 60 age group than in the other groups (p < 0.05) . The total seam cholesterol increased with increasing age (p < 0.001), and SBP increased with increasing Body Mass Index (BMI) (p < 0.001). Diastolic blood pressure (DBP), total cholesterol (p < 0.01), hemoglobin (p < 0.05) and glutamate-pyruvate-aminotransferase (GPT) (p < 0.05) were significantly higher in the BMI of over 20, than in the BMI under 20. The GPT was significantly higher in the BMI over 25 than in the BMI under 25. In The mean daily energy intake was 1912.7 kcal (76.5% of the RDA) ultra the protein intake was 74.3 g (106.2% or the RDA). Compared with the recommended allowance, the energy, calcium, thiamin, and riboflavin intakes were below, and the Fe, vitamin A, vitamin C intakes were above the Korean RDA. The average carbohydrate, protein, fat ratio of energy intake was 66 : 15 : 19, respectively. The length of their driving careers had a positive correlation with their ages and total serum cholesterol levels, and their ages had a positive correlation with their blood pressures, total seam cholesterol and hemoglobin levels and BMI. Their BMI had a positive correlation with their ages, blood pressures, total serum cholesterol, hemoglobin, GPT and GOT levels. The results of this study indicate that bus drivers needed regular exercise, and control of their body weights to prevent chronic diseases, and that their health status should be monitored as the length of their driving careers and ages increase.

• Asian-Australasian Journal of Animal Sciences
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• 제15권1호
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• pp.79-83
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• 2002

The growth and immune responses to endotoxin lipopolysaccharide (LPS) challenge ( $20{\mu}g/kg$) of piglets with and without a iron dextran injection (Fe, 200 mg/head) two days after birth are compared. Sixty-four newborn piglets from eight litters were allocated randomly to one of four treatments. The control received no iron dextran and only saline (Sal) injection on the second and fifteenth day after birth (Sal-Sal). The remaining three groups received Fe-Sal, Sal-LPS, Fe-LPS treatments respectively. On fifteen days of age, blood samples of piglets were taken at 0 h, 1 h, 2 h and 4 d after saline or LPS injection to determine immune functions and blood characteristics. The trial terminated when the pig reached 56 days and the average daily gain of piglets was then measured. Daily gain, serum immunoglobulin G (IgG) concentration and red blood cell counts did not vary significantly among the four groups at any measuring times. Serum tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) concentration increased sharply 1 h after LPS injection. However, iron injection did not change TNF-${\alpha}$ concentration responds to LPS injection. White blood cell counts of two LPS injection groups were significantly lowered 1 h following the injection. In contrast, serum lactoferrin concentration had increased significantly 1 and 2 h postinjection. Furthermore, iron injection produced no further effects on these two criteria. Iron injection increased the hemoglobin (Hb) concentration of piglets at any measuring time, and LPS injection lowered Hb concentration. In conclusion, a 200 mg/head of iron dextran injection on the second day after birth increased Hb concentration, had no detrimental effect on the immune responses and growth of piglets. Moreover, if creep feed (175 mg Fe/kg feed) is provided from d 7 after birth, the Fe-injection does not contribute to overall performance of piglets and may not be a necessity in practice.